Mutagenetix > Incidental Mutation

Incidental Mutation 'R5671:Ifna6'

442609

Institutional Source

Beutler Lab

Gene Symbol

Ifna6

Ensembl Gene

ENSMUSG00000101252

Gene Name

interferon alpha 6

Synonyms

Ifa6, Ifna8

MMRRC Submission

043314-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R5671 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

88745653-88746222 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 88745906 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 85 (Q85L)

Ref Sequence

ENSEMBL: ENSMUSP00000100777 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105146]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000105146
AA Change: Q85L

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000100777
Gene: ENSMUSG00000101252
AA Change: Q85L

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	5.8e-71	SMART

Meta Mutation Damage Score

0.5324

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

100% (57/57)

MGI Phenotype

PHENOTYPE: Mice heterozygous for reporter allele are viable and fertile, and show normal production of interferon-alpha in response to viral infection. [provided by MGI curators]

Allele List at MGI

All alleles(1) : Targeted(1)

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
Ak2	T	A	4: 128,902,040 (GRCm39)	F238I	probably damaging	Het
Akap8l	T	C	17: 32,557,266 (GRCm39)	Y115C	probably damaging	Het
Alms1	T	A	6: 85,606,190 (GRCm39)	N2613K	possibly damaging	Het
Antxr1	C	T	6: 87,194,255 (GRCm39)		probably null	Het
Ap3b1	A	T	13: 94,664,765 (GRCm39)	R901S	unknown	Het
Arhgap45	A	G	10: 79,861,310 (GRCm39)	E491G	probably damaging	Het
Capn11	C	T	17: 45,950,600 (GRCm39)	R293Q	possibly damaging	Het
Cdx1	C	T	18: 61,152,971 (GRCm39)	V212I	probably benign	Het
Clca4b	G	A	3: 144,627,624 (GRCm39)	T449I	probably benign	Het
Clec14a	T	C	12: 58,314,612 (GRCm39)	I337V	probably benign	Het
Clip4	A	C	17: 72,096,878 (GRCm39)	M1L	probably damaging	Het
Exoc3l4	T	C	12: 111,389,851 (GRCm39)	I142T	probably damaging	Het
Flnb	T	A	14: 7,890,843 (GRCm38)	I575N	probably benign	Het
Gad1-ps	A	T	10: 99,280,395 (GRCm39)		noncoding transcript	Het
Golga7	C	T	8: 23,740,360 (GRCm39)	A57T	probably damaging	Het
Gpa33	A	G	1: 165,974,360 (GRCm39)	T66A	possibly damaging	Het
Gpr141b	A	G	13: 19,913,465 (GRCm39)		noncoding transcript	Het
Gpr45	A	G	1: 43,072,218 (GRCm39)	Y287C	probably damaging	Het
H2-Eb1	C	A	17: 34,533,229 (GRCm39)	Y150*	probably null	Het
Hsd17b8	T	C	17: 34,245,435 (GRCm39)	D233G	probably null	Het
Igkv9-120	G	A	6: 68,027,257 (GRCm39)	W57*	probably null	Het
Ivns1abp	G	T	1: 151,229,760 (GRCm39)	L149F	probably benign	Het
Kank4	A	G	4: 98,653,698 (GRCm39)		probably null	Het
Lama2	A	T	10: 27,066,540 (GRCm39)	C1114S	probably damaging	Het
Lmbr1l	A	C	15: 98,805,489 (GRCm39)	D337E	possibly damaging	Het
Ly75	T	C	2: 60,138,655 (GRCm39)	D1404G	probably damaging	Het
Muc4	A	G	16: 32,575,011 (GRCm39)	T1199A	probably benign	Het
Nalcn	A	G	14: 123,532,818 (GRCm39)	I1314T	probably damaging	Het
Nhlrc1	A	G	13: 47,167,193 (GRCm39)	F355L	probably benign	Het
Or2y16	T	C	11: 49,335,140 (GRCm39)	V154A	probably benign	Het
Or4k39	C	T	2: 111,238,818 (GRCm39)		noncoding transcript	Het
Or5b21	A	T	19: 12,839,171 (GRCm39)	T11S	probably benign	Het
Pcsk1	A	G	13: 75,246,026 (GRCm39)	T135A	possibly damaging	Het
Ptpru	T	A	4: 131,547,501 (GRCm39)	Y112F	possibly damaging	Het
Rbbp8	A	G	18: 11,875,699 (GRCm39)	S871G	probably benign	Het
Rhoj	A	T	12: 75,440,743 (GRCm39)	I135F	probably damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Semp2l2b	T	A	10: 21,942,742 (GRCm39)	T413S	possibly damaging	Het
Ska1	G	T	18: 74,330,067 (GRCm39)	D224E	probably damaging	Het
Slc11a2	A	G	15: 100,301,169 (GRCm39)	Y295H	probably damaging	Het
Slc22a28	A	T	19: 8,108,795 (GRCm39)	C116S	probably damaging	Het
Synpo	T	C	18: 60,729,022 (GRCm39)	D1060G	probably damaging	Het
Tectb	T	C	19: 55,181,059 (GRCm39)	S310P	probably benign	Het
Tmc6	A	G	11: 117,666,441 (GRCm39)	S288P	possibly damaging	Het
Tpo	A	G	12: 30,169,490 (GRCm39)	S82P	probably benign	Het
Ttyh3	T	A	5: 140,617,307 (GRCm39)	M321L	probably benign	Het
Usp8	A	G	2: 126,584,345 (GRCm39)	D518G	probably benign	Het
Vmn2r11	A	G	5: 109,202,772 (GRCm39)	W102R	probably benign	Het
Vps13a	G	A	19: 16,692,464 (GRCm39)	H817Y	probably benign	Het
Vps51	A	G	19: 6,118,224 (GRCm39)	V757A	probably benign	Het
Washc4	T	C	10: 83,405,892 (GRCm39)	S463P	probably damaging	Het
Zfp423	T	C	8: 88,508,955 (GRCm39)	N442S	probably damaging	Het

Other mutations in Ifna6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03403:Ifna6	APN	4	88,745,695 (GRCm39)	missense	possibly damaging	0.69
R0336:Ifna6	UTSW	4	88,746,178 (GRCm39)	missense	probably damaging	0.97
R0521:Ifna6	UTSW	4	88,745,887 (GRCm39)	missense	probably benign	0.30
R2863:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R2863:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R2865:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R2865:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R4171:Ifna6	UTSW	4	88,746,038 (GRCm39)	missense	probably benign	0.06
R4534:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R4534:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R4992:Ifna6	UTSW	4	88,745,777 (GRCm39)	missense	probably benign	0.00
R5667:Ifna6	UTSW	4	88,745,906 (GRCm39)	missense	probably damaging	1.00
R5677:Ifna6	UTSW	4	88,745,956 (GRCm39)	missense	probably benign	0.00
R6487:Ifna6	UTSW	4	88,745,743 (GRCm39)	missense	probably damaging	1.00
R6751:Ifna6	UTSW	4	88,745,987 (GRCm39)	missense	probably damaging	1.00
R7424:Ifna6	UTSW	4	88,746,044 (GRCm39)	missense	possibly damaging	0.53
R7711:Ifna6	UTSW	4	88,745,971 (GRCm39)	missense	probably benign	0.06
R8850:Ifna6	UTSW	4	88,746,222 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- TACTGGTCAACCTGCTCTCTAG -3'
(R):5'- TGTGGAAGTATGTCCTCACAGC -3'

Sequencing Primer
(F):5'- AACCTGCTCTCTAGGATGTGAC -3'
(R):5'- GTATGTCCTCACAGCCAGCAG -3'

Posted On

2016-11-09