Mutagenetix > Incidental Mutation

Incidental Mutation 'R5677:Mink1'

442824

Institutional Source

Beutler Lab

Gene Symbol

Mink1

Ensembl Gene

ENSMUSG00000020827

Gene Name

misshapen-like kinase 1 (zebrafish)

Synonyms

Misshapen/NIKs-related kinase, Map4k6, Ysk2, MINK

MMRRC Submission

043316-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5677 (G1)

Quality Score

182

Status

Not validated

Chromosome

Chromosomal Location

70453707-70505309 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 70495991 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Leucine at position 75 (R75L)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072237] [ENSMUST00000072873] [ENSMUST00000079244] [ENSMUST00000102558] [ENSMUST00000102559]

AlphaFold

Q9JM52

Predicted Effect

possibly damaging
Transcript: ENSMUST00000072237
AA Change: R397L

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000072091
Gene: ENSMUSG00000020827
AA Change: R397L

Domain	Start	End	E-Value	Type
S_TKc	25	289	1.86e-91	SMART
low complexity region	307	338	N/A	INTRINSIC
coiled coil region	351	496	N/A	INTRINSIC
low complexity region	557	569	N/A	INTRINSIC
low complexity region	620	633	N/A	INTRINSIC
low complexity region	646	659	N/A	INTRINSIC
low complexity region	719	738	N/A	INTRINSIC
low complexity region	837	874	N/A	INTRINSIC
CNH	1026	1324	1.58e-113	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000072873
AA Change: R397L

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000072649
Gene: ENSMUSG00000020827
AA Change: R397L

Domain	Start	End	E-Value	Type
S_TKc	25	289	1.86e-91	SMART
low complexity region	307	338	N/A	INTRINSIC
coiled coil region	351	496	N/A	INTRINSIC
low complexity region	557	569	N/A	INTRINSIC
low complexity region	620	633	N/A	INTRINSIC
low complexity region	646	659	N/A	INTRINSIC
low complexity region	719	738	N/A	INTRINSIC
low complexity region	829	853	N/A	INTRINSIC
CNH	1019	1317	1.58e-113	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000079244
AA Change: R394L

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000078234
Gene: ENSMUSG00000020827
AA Change: R394L

Domain	Start	End	E-Value	Type
S_TKc	25	289	1.86e-91	SMART
low complexity region	314	338	N/A	INTRINSIC
coiled coil region	348	493	N/A	INTRINSIC
low complexity region	554	566	N/A	INTRINSIC
low complexity region	617	630	N/A	INTRINSIC
low complexity region	643	656	N/A	INTRINSIC
low complexity region	716	735	N/A	INTRINSIC
low complexity region	826	850	N/A	INTRINSIC
CNH	1016	1314	1.58e-113	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000102558
AA Change: R397L

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000099618
Gene: ENSMUSG00000020827
AA Change: R397L

Domain	Start	End	E-Value	Type
S_TKc	25	289	1.86e-91	SMART
low complexity region	307	338	N/A	INTRINSIC
coiled coil region	351	496	N/A	INTRINSIC
low complexity region	557	569	N/A	INTRINSIC
low complexity region	620	633	N/A	INTRINSIC
low complexity region	646	659	N/A	INTRINSIC
low complexity region	792	816	N/A	INTRINSIC
CNH	982	1280	1.58e-113	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000102559
AA Change: R397L

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000099619
Gene: ENSMUSG00000020827
AA Change: R397L

Domain	Start	End	E-Value	Type
S_TKc	25	289	1.86e-91	SMART
low complexity region	307	338	N/A	INTRINSIC
coiled coil region	351	496	N/A	INTRINSIC
low complexity region	557	569	N/A	INTRINSIC
low complexity region	620	633	N/A	INTRINSIC
low complexity region	646	659	N/A	INTRINSIC
low complexity region	800	824	N/A	INTRINSIC
CNH	990	1288	1.58e-113	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132208

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133310

Predicted Effect

unknown
Transcript: ENSMUST00000149845
AA Change: R67L

Predicted Effect

probably benign
Transcript: ENSMUST00000136663
AA Change: R250L

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000117959
Gene: ENSMUSG00000020827
AA Change: R250L

Domain	Start	End	E-Value	Type
Pfam:Pkinase_Tyr	1	140	2.3e-22	PFAM
Pfam:Pkinase	1	143	1.6e-30	PFAM
low complexity region	161	192	N/A	INTRINSIC
coiled coil region	204	349	N/A	INTRINSIC
low complexity region	411	423	N/A	INTRINSIC
low complexity region	474	487	N/A	INTRINSIC
low complexity region	500	513	N/A	INTRINSIC
low complexity region	573	592	N/A	INTRINSIC
low complexity region	691	728	N/A	INTRINSIC
CNH	880	1178	1.58e-113	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000178764
AA Change: R75L

PolyPhen 2 Score 0.663 (Sensitivity: 0.86; Specificity: 0.91)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153503

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152857

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142650

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a serine/threonine kinase belonging to the germinal center kinase (GCK) family. The protein is structurally similar to the kinases that are related to NIK and may belong to a distinct subfamily of NIK-related kinases within the GCK family. Studies of the mouse homolog indicate an up-regulation of expression in the course of postnatal mouse cerebral development and activation of the cJun N-terminal kinase (JNK) and the p38 pathways. [provided by RefSeq, Mar 2016]

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057J18Rik	C	T	10: 28,862,225 (GRCm39)	V22I	probably benign	Het
Abca8a	A	T	11: 109,929,225 (GRCm39)	V1296D	possibly damaging	Het
Abca8b	C	T	11: 109,831,687 (GRCm39)	S1328N	probably damaging	Het
Adcy1	G	A	11: 7,111,914 (GRCm39)	M926I	probably damaging	Het
Aff4	G	T	11: 53,291,102 (GRCm39)	M687I	possibly damaging	Het
Agbl2	T	A	2: 90,638,322 (GRCm39)	Y636N	possibly damaging	Het
Agtr1a	A	T	13: 30,565,567 (GRCm39)	I211F	probably damaging	Het
Alkbh8	T	G	9: 3,385,147 (GRCm39)	S480A	possibly damaging	Het
Ankrd13b	A	T	11: 77,368,370 (GRCm39)	V84E	probably damaging	Het
Ap3b1	C	T	13: 94,664,704 (GRCm39)	T881I	unknown	Het
Apbb1	A	C	7: 105,208,453 (GRCm39)	D617E	probably damaging	Het
Apobec4	A	G	1: 152,633,033 (GRCm39)	R354G	probably benign	Het
Brdt	T	G	5: 107,496,483 (GRCm39)	C198W	possibly damaging	Het
Cacna1b	G	T	2: 24,569,370 (GRCm39)	H851Q	possibly damaging	Het
Car2	G	T	3: 14,963,115 (GRCm39)	V217F	possibly damaging	Het
Ccdc24	A	C	4: 117,727,077 (GRCm39)		probably benign	Het
Chodl	C	A	16: 78,738,203 (GRCm39)	A57E	probably damaging	Het
Clgn	G	T	8: 84,136,167 (GRCm39)	C185F	probably damaging	Het
Cltc	G	T	11: 86,596,068 (GRCm39)	N1223K	probably damaging	Het
Cnot10	T	C	9: 114,458,161 (GRCm39)	N115S	probably damaging	Het
Col12a1	T	C	9: 79,606,603 (GRCm39)	R607G	probably damaging	Het
Cpd	A	G	11: 76,690,651 (GRCm39)	V835A	probably benign	Het
Cplane2	C	T	4: 140,947,177 (GRCm39)	P186L	probably benign	Het
Csmd3	G	C	15: 48,485,447 (GRCm39)	L153V	probably damaging	Het
Ctr9	A	G	7: 110,643,209 (GRCm39)	H527R	probably benign	Het
Cwc22	G	A	2: 77,759,787 (GRCm39)	R87W	probably damaging	Het
D930020B18Rik	T	A	10: 121,505,106 (GRCm39)	N107K	probably benign	Het
Dgkg	C	A	16: 22,388,921 (GRCm39)	V418L	probably benign	Het
Dhx40	A	G	11: 86,691,789 (GRCm39)		probably null	Het
Diaph1	A	T	18: 37,989,004 (GRCm39)	M910K	probably damaging	Het
Diras2	T	A	13: 52,661,711 (GRCm39)	M199L	possibly damaging	Het
Dnah8	G	A	17: 30,967,542 (GRCm39)	D2585N	probably benign	Het
Dock5	T	A	14: 68,015,052 (GRCm39)	Q1302H	probably benign	Het
Dync1i2	T	C	2: 71,058,967 (GRCm39)	S90P	probably benign	Het
E2f8	A	T	7: 48,516,943 (GRCm39)	V812E	probably damaging	Het
Egfem1	C	A	3: 29,744,323 (GRCm39)	Q521K	probably damaging	Het
Fbxl18	A	T	5: 142,864,475 (GRCm39)	C699*	probably null	Het
Fgf3	C	T	7: 144,392,520 (GRCm39)	R26*	probably null	Het
Fpr-rs7	T	G	17: 20,334,365 (GRCm39)	I42L	probably benign	Het
Gm3159	T	A	14: 4,398,582 (GRCm38)	M91K	probably damaging	Het
Gprin3	A	G	6: 59,330,877 (GRCm39)	S477P	possibly damaging	Het
Grm8	A	T	6: 27,761,203 (GRCm39)		probably null	Het
Hepacam2	A	T	6: 3,466,142 (GRCm39)	D420E	probably damaging	Het
Hmcn1	A	T	1: 150,485,529 (GRCm39)	W4358R	probably benign	Het
Ifna6	G	A	4: 88,745,956 (GRCm39)	A102T	probably benign	Het
Ighv2-2	T	C	12: 113,552,142 (GRCm39)	Q32R	probably benign	Het
Igkv1-131	T	A	6: 67,743,242 (GRCm39)	Q47L	possibly damaging	Het
Il16	T	C	7: 83,323,761 (GRCm39)	E263G	probably damaging	Het
Kansl1	A	T	11: 104,225,974 (GRCm39)	C981S	probably benign	Het
Lrp1	A	T	10: 127,410,298 (GRCm39)	F1483I	probably damaging	Het
Ltf	T	C	9: 110,849,980 (GRCm39)	M1T	probably null	Het
Ly75	C	T	2: 60,129,426 (GRCm39)	R1653H	probably benign	Het
Macrod2	T	C	2: 142,018,587 (GRCm39)	F240S	probably damaging	Het
Man1c1	T	C	4: 134,296,371 (GRCm39)	E433G	probably damaging	Het
Mansc4	A	T	6: 146,983,047 (GRCm39)	M130K	probably benign	Het
Mccc1	C	T	3: 36,044,197 (GRCm39)		probably null	Het
Mst1	A	G	9: 107,958,485 (GRCm39)	D65G	probably damaging	Het
Myo9b	C	T	8: 71,796,330 (GRCm39)	A857V	probably damaging	Het
Ndufa4	A	G	6: 11,900,574 (GRCm39)	V70A	probably benign	Het
Npat	T	A	9: 53,466,400 (GRCm39)	S230T	probably benign	Het
Nr1d1	A	G	11: 98,662,134 (GRCm39)	Y167H	probably damaging	Het
Oca2	A	G	7: 56,064,210 (GRCm39)	D735G	probably damaging	Het
Odr4	G	A	1: 150,250,588 (GRCm39)	L319F	probably damaging	Het
Or2a7	T	C	6: 43,151,265 (GRCm39)	V115A	probably benign	Het
Or2b2	C	T	13: 21,887,393 (GRCm39)	T74I	probably benign	Het
Or2y13	A	G	11: 49,414,771 (GRCm39)	T74A	probably damaging	Het
Or7g20	T	A	9: 18,946,854 (GRCm39)	I145N	possibly damaging	Het
Otop1	A	G	5: 38,457,507 (GRCm39)	Y422C	probably damaging	Het
Pde4dip	G	A	3: 97,748,964 (GRCm39)	R126*	probably null	Het
Pdp2	C	T	8: 105,321,320 (GRCm39)	P390S	probably damaging	Het
Pds5b	A	T	5: 150,639,926 (GRCm39)	T14S	possibly damaging	Het
Pfkp	T	C	13: 6,638,631 (GRCm39)	E580G	probably damaging	Het
Piezo2	A	T	18: 63,250,767 (GRCm39)	L212Q	possibly damaging	Het
Piezo2	G	C	18: 63,250,768 (GRCm39)	L444V	probably benign	Het
Pla2g4d	T	A	2: 120,109,429 (GRCm39)	T207S	possibly damaging	Het
Plk2	C	A	13: 110,535,591 (GRCm39)	T471K	possibly damaging	Het
Potefam1	T	C	2: 111,041,910 (GRCm39)	T342A	probably benign	Het
Potegl	T	A	2: 23,102,730 (GRCm39)	L156Q	probably damaging	Het
Ppp1r3g	G	A	13: 36,153,245 (GRCm39)	E222K	probably damaging	Het
Pramel28	A	T	4: 143,691,708 (GRCm39)	D338E	possibly damaging	Het
Prkcg	A	G	7: 3,371,974 (GRCm39)	D480G	probably damaging	Het
Pxdc1	T	A	13: 34,836,178 (GRCm39)	T81S	probably benign	Het
Rnf150	A	T	8: 83,730,228 (GRCm39)	K253*	probably null	Het
Sae1	T	C	7: 16,104,387 (GRCm39)		probably null	Het
Scin	C	T	12: 40,113,258 (GRCm39)	D538N	probably damaging	Het
Serpinb3c	C	A	1: 107,199,533 (GRCm39)	K329N	probably damaging	Het
Sgo2b	T	A	8: 64,380,008 (GRCm39)	K941N	possibly damaging	Het
Six1	C	T	12: 73,093,058 (GRCm39)	S48N	possibly damaging	Het
Slc39a8	G	A	3: 135,590,449 (GRCm39)	G381R	probably damaging	Het
Slc9b1	A	G	3: 135,063,320 (GRCm39)	K35E	unknown	Het
Srek1	C	T	13: 103,895,752 (GRCm39)	A274T	probably damaging	Het
Steap2	A	T	5: 5,727,497 (GRCm39)	Y279*	probably null	Het
Svil	T	A	18: 5,046,823 (GRCm39)	L110*	probably null	Het
Syncrip	T	C	9: 88,338,762 (GRCm39)		probably benign	Het
Tcf20	A	G	15: 82,737,443 (GRCm39)	I1336T	probably benign	Het
Tecpr1	T	A	5: 144,155,451 (GRCm39)	K36*	probably null	Het
Tenm2	A	G	11: 36,032,510 (GRCm39)	V670A	probably damaging	Het
Thbd	G	A	2: 148,249,286 (GRCm39)	T194I	probably damaging	Het
Tm9sf2	A	T	14: 122,389,374 (GRCm39)		probably null	Het
Tmtc4	A	T	14: 123,187,911 (GRCm39)	I225N	probably damaging	Het
Tpp1	C	T	7: 105,396,743 (GRCm39)	V425M	probably damaging	Het
Trbc2	T	A	6: 41,524,746 (GRCm39)	Y144*	probably null	Het
Trps1	T	C	15: 50,709,504 (GRCm39)	D282G	probably damaging	Het
Tsc22d4	T	A	5: 137,745,404 (GRCm39)	S9R	probably damaging	Het
Upp1	T	A	11: 9,086,025 (GRCm39)	D287E	probably benign	Het
Uso1	A	C	5: 92,349,158 (GRCm39)	Q916H	probably damaging	Het
Uty	T	A	Y: 1,134,902 (GRCm39)	Y884F	probably damaging	Het
Vmn1r222	T	C	13: 23,416,950 (GRCm39)	R88G	probably damaging	Het
Vmn1r79	T	C	7: 11,910,928 (GRCm39)	V270A	possibly damaging	Het
Zbtb22	T	C	17: 34,136,709 (GRCm39)	S285P	probably benign	Het
Zfp385a	A	G	15: 103,226,492 (GRCm39)	V82A	probably damaging	Het
Zfp59	T	C	7: 27,553,594 (GRCm39)	F349L	probably benign	Het
Zfp780b	T	G	7: 27,662,224 (GRCm39)	H777P	probably benign	Het
Zfp82	T	C	7: 29,756,549 (GRCm39)	T178A	probably benign	Het
Zfp850	T	C	7: 27,688,513 (GRCm39)	Y565C	probably damaging	Het
Zfp957	A	G	14: 79,450,207 (GRCm39)	Y531H	probably damaging	Het

Other mutations in Mink1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00501:Mink1	APN	11	70,494,638 (GRCm39)	missense	probably damaging	0.99
IGL00709:Mink1	APN	11	70,503,845 (GRCm39)	missense	probably damaging	0.99
IGL01064:Mink1	APN	11	70,494,307 (GRCm39)	missense	probably benign	0.05
IGL02612:Mink1	APN	11	70,488,052 (GRCm39)	missense	probably damaging	1.00
IGL02797:Mink1	APN	11	70,501,176 (GRCm39)	missense	probably damaging	1.00
IGL03056:Mink1	APN	11	70,503,409 (GRCm39)	critical splice donor site	probably null
IGL03066:Mink1	APN	11	70,499,715 (GRCm39)	missense	probably benign	0.01
IGL03185:Mink1	APN	11	70,494,686 (GRCm39)	missense	probably damaging	1.00
PIT4498001:Mink1	UTSW	11	70,489,714 (GRCm39)	missense	probably benign	0.05
R0025:Mink1	UTSW	11	70,503,868 (GRCm39)	missense	probably damaging	1.00
R0025:Mink1	UTSW	11	70,503,868 (GRCm39)	missense	probably damaging	1.00
R0488:Mink1	UTSW	11	70,488,030 (GRCm39)	missense	probably damaging	1.00
R0637:Mink1	UTSW	11	70,492,502 (GRCm39)	missense	probably damaging	0.96
R0828:Mink1	UTSW	11	70,500,971 (GRCm39)	nonsense	probably null
R1081:Mink1	UTSW	11	70,497,861 (GRCm39)	missense	probably benign	0.07
R1175:Mink1	UTSW	11	70,502,166 (GRCm39)	missense	probably benign	0.02
R1441:Mink1	UTSW	11	70,497,940 (GRCm39)	missense	possibly damaging	0.72
R1532:Mink1	UTSW	11	70,492,833 (GRCm39)	missense	probably null	1.00
R1545:Mink1	UTSW	11	70,489,717 (GRCm39)	missense	possibly damaging	0.60
R1634:Mink1	UTSW	11	70,499,706 (GRCm39)	missense	probably benign	0.00
R1932:Mink1	UTSW	11	70,499,254 (GRCm39)	critical splice donor site	probably null
R2033:Mink1	UTSW	11	70,503,334 (GRCm39)	missense	probably damaging	1.00
R2184:Mink1	UTSW	11	70,494,623 (GRCm39)	missense	probably damaging	1.00
R2267:Mink1	UTSW	11	70,492,550 (GRCm39)	splice site	probably null
R2268:Mink1	UTSW	11	70,492,550 (GRCm39)	splice site	probably null
R2859:Mink1	UTSW	11	70,503,334 (GRCm39)	missense	probably damaging	1.00
R3713:Mink1	UTSW	11	70,499,776 (GRCm39)	missense	possibly damaging	0.93
R3714:Mink1	UTSW	11	70,499,776 (GRCm39)	missense	possibly damaging	0.93
R3715:Mink1	UTSW	11	70,499,776 (GRCm39)	missense	possibly damaging	0.93
R3716:Mink1	UTSW	11	70,498,587 (GRCm39)	missense	probably damaging	0.98
R3717:Mink1	UTSW	11	70,498,587 (GRCm39)	missense	probably damaging	0.98
R4607:Mink1	UTSW	11	70,496,893 (GRCm39)	missense	possibly damaging	0.72
R4735:Mink1	UTSW	11	70,500,086 (GRCm39)	splice site	probably null
R4790:Mink1	UTSW	11	70,489,867 (GRCm39)	missense	probably damaging	0.99
R4847:Mink1	UTSW	11	70,492,854 (GRCm39)	missense	probably damaging	1.00
R4860:Mink1	UTSW	11	70,502,418 (GRCm39)	missense	probably damaging	0.98
R4860:Mink1	UTSW	11	70,502,418 (GRCm39)	missense	probably damaging	0.98
R5081:Mink1	UTSW	11	70,495,970 (GRCm39)	missense	probably damaging	0.98
R5310:Mink1	UTSW	11	70,498,169 (GRCm39)	missense	probably benign	0.33
R5767:Mink1	UTSW	11	70,496,901 (GRCm39)	missense	possibly damaging	0.53
R5795:Mink1	UTSW	11	70,498,616 (GRCm39)	missense	possibly damaging	0.86
R5888:Mink1	UTSW	11	70,500,885 (GRCm39)	unclassified	probably benign
R5950:Mink1	UTSW	11	70,500,412 (GRCm39)	missense	possibly damaging	0.81
R6024:Mink1	UTSW	11	70,489,915 (GRCm39)	missense	possibly damaging	0.71
R6034:Mink1	UTSW	11	70,497,866 (GRCm39)	small deletion	probably benign
R6034:Mink1	UTSW	11	70,497,866 (GRCm39)	small deletion	probably benign
R6058:Mink1	UTSW	11	70,502,546 (GRCm39)	missense	possibly damaging	0.96
R6144:Mink1	UTSW	11	70,501,478 (GRCm39)	missense	possibly damaging	0.66
R6154:Mink1	UTSW	11	70,500,927 (GRCm39)	missense	possibly damaging	0.46
R6218:Mink1	UTSW	11	70,489,720 (GRCm39)	missense	possibly damaging	0.94
R6262:Mink1	UTSW	11	70,494,151 (GRCm39)	splice site	probably null
R6269:Mink1	UTSW	11	70,489,813 (GRCm39)	missense	probably damaging	1.00
R6273:Mink1	UTSW	11	70,502,261 (GRCm39)	nonsense	probably null
R6301:Mink1	UTSW	11	70,503,120 (GRCm39)	missense	possibly damaging	0.71
R6603:Mink1	UTSW	11	70,500,419 (GRCm39)	missense	probably damaging	0.96
R6876:Mink1	UTSW	11	70,498,261 (GRCm39)	missense	probably benign	0.02
R7030:Mink1	UTSW	11	70,498,601 (GRCm39)	missense	possibly damaging	0.46
R7050:Mink1	UTSW	11	70,503,158 (GRCm39)	missense	possibly damaging	0.93
R7094:Mink1	UTSW	11	70,500,901 (GRCm39)	splice site	probably null
R7135:Mink1	UTSW	11	70,494,329 (GRCm39)	missense	probably damaging	1.00
R7238:Mink1	UTSW	11	70,502,305 (GRCm39)	critical splice donor site	probably null
R7320:Mink1	UTSW	11	70,489,899 (GRCm39)	missense	probably benign	0.23
R7396:Mink1	UTSW	11	70,495,994 (GRCm39)	missense	possibly damaging	0.73
R7446:Mink1	UTSW	11	70,500,455 (GRCm39)	missense	probably benign	0.18
R7723:Mink1	UTSW	11	70,503,736 (GRCm39)	missense	probably benign	0.16
R7896:Mink1	UTSW	11	70,503,108 (GRCm39)	missense	possibly damaging	0.71
R8058:Mink1	UTSW	11	70,494,594 (GRCm39)	nonsense	probably null
R8082:Mink1	UTSW	11	70,504,103 (GRCm39)	missense	possibly damaging	0.71
R8160:Mink1	UTSW	11	70,496,907 (GRCm39)	nonsense	probably null
R8335:Mink1	UTSW	11	70,500,401 (GRCm39)	missense	probably damaging	0.97
R8353:Mink1	UTSW	11	70,501,154 (GRCm39)	missense	possibly damaging	0.70
R8453:Mink1	UTSW	11	70,501,154 (GRCm39)	missense	possibly damaging	0.70
R8732:Mink1	UTSW	11	70,500,902 (GRCm39)	critical splice acceptor site	probably null
R9072:Mink1	UTSW	11	70,499,207 (GRCm39)	missense	possibly damaging	0.86
R9073:Mink1	UTSW	11	70,499,207 (GRCm39)	missense	possibly damaging	0.86
R9324:Mink1	UTSW	11	70,502,477 (GRCm39)	missense	probably damaging	0.98
R9596:Mink1	UTSW	11	70,497,915 (GRCm39)	missense	possibly damaging	0.96

Predicted Primers

PCR Primer
(F):5'- ATCATGAATGTGCCCGGTG -3'
(R):5'- TGTCAACCTTTGGCCAACAG -3'

Sequencing Primer
(F):5'- ATGAATGTGCCCGGTGAGTCC -3'
(R):5'- CAGAAAAGCAACCTTTGGCTTTGG -3'

Posted On

2016-11-09