Mutagenetix > Incidental Mutation

Incidental Mutation 'R5677:Trps1'

442851

Institutional Source

Beutler Lab

Gene Symbol

Trps1

Ensembl Gene

ENSMUSG00000038679

Gene Name

transcriptional repressor GATA binding 1

Synonyms

D15Ertd586e, trichorhinophalangeal syndrome I (human)

MMRRC Submission

043316-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5677 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

50518148-50753859 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 50709504 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 282 (D282G)

Ref Sequence

ENSEMBL: ENSMUSP00000139017 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077935] [ENSMUST00000165201] [ENSMUST00000183757] [ENSMUST00000183997] [ENSMUST00000184458] [ENSMUST00000184885] [ENSMUST00000185183]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000077935
AA Change: D278G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000077089
Gene: ENSMUSG00000038679
AA Change: D278G

Domain	Start	End	E-Value	Type
ZnF_C2H2	222	247	1.41e0	SMART
ZnF_C2H2	333	358	4.45e0	SMART
ZnF_C2H2	434	459	1.31e2	SMART
ZnF_C2H2	523	554	1.93e2	SMART
low complexity region	597	602	N/A	INTRINSIC
ZnF_C2H2	614	637	8.67e-1	SMART
ZnF_C2H2	666	689	2.29e0	SMART
ZnF_C2H2	692	715	8.22e-2	SMART
low complexity region	766	779	N/A	INTRINSIC
ZnF_GATA	890	940	3.95e-16	SMART
low complexity region	1050	1062	N/A	INTRINSIC
ZnF_C2H2	1215	1237	4.34e0	SMART
ZnF_C2H2	1243	1267	5.72e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000165201
AA Change: D278G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000129779
Gene: ENSMUSG00000038679
AA Change: D278G

Domain	Start	End	E-Value	Type
ZnF_C2H2	222	247	1.41e0	SMART
ZnF_C2H2	333	358	4.45e0	SMART
ZnF_C2H2	434	459	1.31e2	SMART
ZnF_C2H2	523	554	1.93e2	SMART
low complexity region	597	602	N/A	INTRINSIC
ZnF_C2H2	614	637	8.67e-1	SMART
ZnF_C2H2	666	689	2.29e0	SMART
ZnF_C2H2	692	715	8.22e-2	SMART
low complexity region	766	779	N/A	INTRINSIC
ZnF_GATA	890	940	3.95e-16	SMART
low complexity region	1050	1062	N/A	INTRINSIC
ZnF_C2H2	1215	1237	4.34e0	SMART
ZnF_C2H2	1243	1267	5.72e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000183757
AA Change: D282G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000139017
Gene: ENSMUSG00000038679
AA Change: D282G

Domain	Start	End	E-Value	Type
ZnF_C2H2	226	251	1.41e0	SMART
ZnF_C2H2	337	362	4.45e0	SMART
ZnF_C2H2	438	463	1.31e2	SMART
ZnF_C2H2	527	558	1.93e2	SMART
low complexity region	601	606	N/A	INTRINSIC
ZnF_C2H2	618	641	8.67e-1	SMART
ZnF_C2H2	670	693	2.29e0	SMART
ZnF_C2H2	696	719	8.22e-2	SMART
low complexity region	770	783	N/A	INTRINSIC
ZnF_GATA	894	944	3.95e-16	SMART
low complexity region	1054	1066	N/A	INTRINSIC
ZnF_C2H2	1219	1241	4.34e0	SMART
ZnF_C2H2	1247	1271	5.72e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000183997
AA Change: D282G

PolyPhen 2 Score 0.845 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000139115
Gene: ENSMUSG00000038679
AA Change: D282G

Domain	Start	End	E-Value	Type
ZnF_C2H2	226	251	1.41e0	SMART
ZnF_C2H2	337	362	4.45e0	SMART
ZnF_C2H2	470	493	2.29e0	SMART
ZnF_C2H2	496	519	8.22e-2	SMART
low complexity region	570	583	N/A	INTRINSIC
ZnF_GATA	705	755	3.95e-16	SMART
low complexity region	865	877	N/A	INTRINSIC
ZnF_C2H2	1030	1052	4.34e0	SMART
ZnF_C2H2	1058	1082	5.72e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000184458

SMART Domains

Protein: ENSMUSP00000139063
Gene: ENSMUSG00000038679

Domain	Start	End	E-Value	Type
ZnF_C2H2	46	71	4.45e0	SMART
ZnF_C2H2	147	172	1.31e2	SMART
ZnF_C2H2	236	267	1.93e2	SMART
low complexity region	310	315	N/A	INTRINSIC
ZnF_C2H2	327	350	8.67e-1	SMART
ZnF_C2H2	379	402	2.29e0	SMART
ZnF_C2H2	405	428	8.22e-2	SMART
low complexity region	479	492	N/A	INTRINSIC
ZnF_GATA	603	653	3.95e-16	SMART
low complexity region	763	775	N/A	INTRINSIC
ZnF_C2H2	928	950	4.34e0	SMART
ZnF_C2H2	956	980	5.72e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000184885
AA Change: D232G

PolyPhen 2 Score 0.845 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000138905
Gene: ENSMUSG00000038679
AA Change: D232G

Domain	Start	End	E-Value	Type
ZnF_C2H2	176	201	1.41e0	SMART
ZnF_C2H2	287	312	4.45e0	SMART
ZnF_C2H2	420	443	2.29e0	SMART
ZnF_C2H2	446	469	8.22e-2	SMART
low complexity region	520	533	N/A	INTRINSIC
ZnF_GATA	644	694	3.95e-16	SMART
low complexity region	804	816	N/A	INTRINSIC
ZnF_C2H2	969	991	4.34e0	SMART
ZnF_C2H2	997	1021	5.72e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000185183

SMART Domains

Protein: ENSMUSP00000138967
Gene: ENSMUSG00000038679

Domain	Start	End	E-Value	Type
ZnF_C2H2	222	247	1.41e0	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a transcription factor that represses GATA-regulated genes and binds to a dynein light chain protein. Binding of the encoded protein to the dynein light chain protein affects binding to GATA consensus sequences and suppresses its transcriptional activity. Defects in this gene are a cause of tricho-rhino-phalangeal syndrome (TRPS) types I-III. [provided by RefSeq, Jul 2008]
PHENOTYPE: Newborn mice homozygous for a knock-out allele exhibit craniofacial and hair anomalies and die of respiratory failure due to thoracic spine and rib defects. Mice homozygous for a reporter allele show additional defects in chondrocyte proliferation and apoptosis as well as reduced nephron formation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057J18Rik	C	T	10: 28,862,225 (GRCm39)	V22I	probably benign	Het
Abca8a	A	T	11: 109,929,225 (GRCm39)	V1296D	possibly damaging	Het
Abca8b	C	T	11: 109,831,687 (GRCm39)	S1328N	probably damaging	Het
Adcy1	G	A	11: 7,111,914 (GRCm39)	M926I	probably damaging	Het
Aff4	G	T	11: 53,291,102 (GRCm39)	M687I	possibly damaging	Het
Agbl2	T	A	2: 90,638,322 (GRCm39)	Y636N	possibly damaging	Het
Agtr1a	A	T	13: 30,565,567 (GRCm39)	I211F	probably damaging	Het
Alkbh8	T	G	9: 3,385,147 (GRCm39)	S480A	possibly damaging	Het
Ankrd13b	A	T	11: 77,368,370 (GRCm39)	V84E	probably damaging	Het
Ap3b1	C	T	13: 94,664,704 (GRCm39)	T881I	unknown	Het
Apbb1	A	C	7: 105,208,453 (GRCm39)	D617E	probably damaging	Het
Apobec4	A	G	1: 152,633,033 (GRCm39)	R354G	probably benign	Het
Brdt	T	G	5: 107,496,483 (GRCm39)	C198W	possibly damaging	Het
Cacna1b	G	T	2: 24,569,370 (GRCm39)	H851Q	possibly damaging	Het
Car2	G	T	3: 14,963,115 (GRCm39)	V217F	possibly damaging	Het
Ccdc24	A	C	4: 117,727,077 (GRCm39)		probably benign	Het
Chodl	C	A	16: 78,738,203 (GRCm39)	A57E	probably damaging	Het
Clgn	G	T	8: 84,136,167 (GRCm39)	C185F	probably damaging	Het
Cltc	G	T	11: 86,596,068 (GRCm39)	N1223K	probably damaging	Het
Cnot10	T	C	9: 114,458,161 (GRCm39)	N115S	probably damaging	Het
Col12a1	T	C	9: 79,606,603 (GRCm39)	R607G	probably damaging	Het
Cpd	A	G	11: 76,690,651 (GRCm39)	V835A	probably benign	Het
Cplane2	C	T	4: 140,947,177 (GRCm39)	P186L	probably benign	Het
Csmd3	G	C	15: 48,485,447 (GRCm39)	L153V	probably damaging	Het
Ctr9	A	G	7: 110,643,209 (GRCm39)	H527R	probably benign	Het
Cwc22	G	A	2: 77,759,787 (GRCm39)	R87W	probably damaging	Het
D930020B18Rik	T	A	10: 121,505,106 (GRCm39)	N107K	probably benign	Het
Dgkg	C	A	16: 22,388,921 (GRCm39)	V418L	probably benign	Het
Dhx40	A	G	11: 86,691,789 (GRCm39)		probably null	Het
Diaph1	A	T	18: 37,989,004 (GRCm39)	M910K	probably damaging	Het
Diras2	T	A	13: 52,661,711 (GRCm39)	M199L	possibly damaging	Het
Dnah8	G	A	17: 30,967,542 (GRCm39)	D2585N	probably benign	Het
Dock5	T	A	14: 68,015,052 (GRCm39)	Q1302H	probably benign	Het
Dync1i2	T	C	2: 71,058,967 (GRCm39)	S90P	probably benign	Het
E2f8	A	T	7: 48,516,943 (GRCm39)	V812E	probably damaging	Het
Egfem1	C	A	3: 29,744,323 (GRCm39)	Q521K	probably damaging	Het
Fbxl18	A	T	5: 142,864,475 (GRCm39)	C699*	probably null	Het
Fgf3	C	T	7: 144,392,520 (GRCm39)	R26*	probably null	Het
Fpr-rs7	T	G	17: 20,334,365 (GRCm39)	I42L	probably benign	Het
Gm3159	T	A	14: 4,398,582 (GRCm38)	M91K	probably damaging	Het
Gprin3	A	G	6: 59,330,877 (GRCm39)	S477P	possibly damaging	Het
Grm8	A	T	6: 27,761,203 (GRCm39)		probably null	Het
Hepacam2	A	T	6: 3,466,142 (GRCm39)	D420E	probably damaging	Het
Hmcn1	A	T	1: 150,485,529 (GRCm39)	W4358R	probably benign	Het
Ifna6	G	A	4: 88,745,956 (GRCm39)	A102T	probably benign	Het
Ighv2-2	T	C	12: 113,552,142 (GRCm39)	Q32R	probably benign	Het
Igkv1-131	T	A	6: 67,743,242 (GRCm39)	Q47L	possibly damaging	Het
Il16	T	C	7: 83,323,761 (GRCm39)	E263G	probably damaging	Het
Kansl1	A	T	11: 104,225,974 (GRCm39)	C981S	probably benign	Het
Lrp1	A	T	10: 127,410,298 (GRCm39)	F1483I	probably damaging	Het
Ltf	T	C	9: 110,849,980 (GRCm39)	M1T	probably null	Het
Ly75	C	T	2: 60,129,426 (GRCm39)	R1653H	probably benign	Het
Macrod2	T	C	2: 142,018,587 (GRCm39)	F240S	probably damaging	Het
Man1c1	T	C	4: 134,296,371 (GRCm39)	E433G	probably damaging	Het
Mansc4	A	T	6: 146,983,047 (GRCm39)	M130K	probably benign	Het
Mccc1	C	T	3: 36,044,197 (GRCm39)		probably null	Het
Mink1	G	T	11: 70,495,991 (GRCm39)	R75L	possibly damaging	Het
Mst1	A	G	9: 107,958,485 (GRCm39)	D65G	probably damaging	Het
Myo9b	C	T	8: 71,796,330 (GRCm39)	A857V	probably damaging	Het
Ndufa4	A	G	6: 11,900,574 (GRCm39)	V70A	probably benign	Het
Npat	T	A	9: 53,466,400 (GRCm39)	S230T	probably benign	Het
Nr1d1	A	G	11: 98,662,134 (GRCm39)	Y167H	probably damaging	Het
Oca2	A	G	7: 56,064,210 (GRCm39)	D735G	probably damaging	Het
Odr4	G	A	1: 150,250,588 (GRCm39)	L319F	probably damaging	Het
Or2a7	T	C	6: 43,151,265 (GRCm39)	V115A	probably benign	Het
Or2b2	C	T	13: 21,887,393 (GRCm39)	T74I	probably benign	Het
Or2y13	A	G	11: 49,414,771 (GRCm39)	T74A	probably damaging	Het
Or7g20	T	A	9: 18,946,854 (GRCm39)	I145N	possibly damaging	Het
Otop1	A	G	5: 38,457,507 (GRCm39)	Y422C	probably damaging	Het
Pde4dip	G	A	3: 97,748,964 (GRCm39)	R126*	probably null	Het
Pdp2	C	T	8: 105,321,320 (GRCm39)	P390S	probably damaging	Het
Pds5b	A	T	5: 150,639,926 (GRCm39)	T14S	possibly damaging	Het
Pfkp	T	C	13: 6,638,631 (GRCm39)	E580G	probably damaging	Het
Piezo2	A	T	18: 63,250,767 (GRCm39)	L212Q	possibly damaging	Het
Piezo2	G	C	18: 63,250,768 (GRCm39)	L444V	probably benign	Het
Pla2g4d	T	A	2: 120,109,429 (GRCm39)	T207S	possibly damaging	Het
Plk2	C	A	13: 110,535,591 (GRCm39)	T471K	possibly damaging	Het
Potefam1	T	C	2: 111,041,910 (GRCm39)	T342A	probably benign	Het
Potegl	T	A	2: 23,102,730 (GRCm39)	L156Q	probably damaging	Het
Ppp1r3g	G	A	13: 36,153,245 (GRCm39)	E222K	probably damaging	Het
Pramel28	A	T	4: 143,691,708 (GRCm39)	D338E	possibly damaging	Het
Prkcg	A	G	7: 3,371,974 (GRCm39)	D480G	probably damaging	Het
Pxdc1	T	A	13: 34,836,178 (GRCm39)	T81S	probably benign	Het
Rnf150	A	T	8: 83,730,228 (GRCm39)	K253*	probably null	Het
Sae1	T	C	7: 16,104,387 (GRCm39)		probably null	Het
Scin	C	T	12: 40,113,258 (GRCm39)	D538N	probably damaging	Het
Serpinb3c	C	A	1: 107,199,533 (GRCm39)	K329N	probably damaging	Het
Sgo2b	T	A	8: 64,380,008 (GRCm39)	K941N	possibly damaging	Het
Six1	C	T	12: 73,093,058 (GRCm39)	S48N	possibly damaging	Het
Slc39a8	G	A	3: 135,590,449 (GRCm39)	G381R	probably damaging	Het
Slc9b1	A	G	3: 135,063,320 (GRCm39)	K35E	unknown	Het
Srek1	C	T	13: 103,895,752 (GRCm39)	A274T	probably damaging	Het
Steap2	A	T	5: 5,727,497 (GRCm39)	Y279*	probably null	Het
Svil	T	A	18: 5,046,823 (GRCm39)	L110*	probably null	Het
Syncrip	T	C	9: 88,338,762 (GRCm39)		probably benign	Het
Tcf20	A	G	15: 82,737,443 (GRCm39)	I1336T	probably benign	Het
Tecpr1	T	A	5: 144,155,451 (GRCm39)	K36*	probably null	Het
Tenm2	A	G	11: 36,032,510 (GRCm39)	V670A	probably damaging	Het
Thbd	G	A	2: 148,249,286 (GRCm39)	T194I	probably damaging	Het
Tm9sf2	A	T	14: 122,389,374 (GRCm39)		probably null	Het
Tmtc4	A	T	14: 123,187,911 (GRCm39)	I225N	probably damaging	Het
Tpp1	C	T	7: 105,396,743 (GRCm39)	V425M	probably damaging	Het
Trbc2	T	A	6: 41,524,746 (GRCm39)	Y144*	probably null	Het
Tsc22d4	T	A	5: 137,745,404 (GRCm39)	S9R	probably damaging	Het
Upp1	T	A	11: 9,086,025 (GRCm39)	D287E	probably benign	Het
Uso1	A	C	5: 92,349,158 (GRCm39)	Q916H	probably damaging	Het
Uty	T	A	Y: 1,134,902 (GRCm39)	Y884F	probably damaging	Het
Vmn1r222	T	C	13: 23,416,950 (GRCm39)	R88G	probably damaging	Het
Vmn1r79	T	C	7: 11,910,928 (GRCm39)	V270A	possibly damaging	Het
Zbtb22	T	C	17: 34,136,709 (GRCm39)	S285P	probably benign	Het
Zfp385a	A	G	15: 103,226,492 (GRCm39)	V82A	probably damaging	Het
Zfp59	T	C	7: 27,553,594 (GRCm39)	F349L	probably benign	Het
Zfp780b	T	G	7: 27,662,224 (GRCm39)	H777P	probably benign	Het
Zfp82	T	C	7: 29,756,549 (GRCm39)	T178A	probably benign	Het
Zfp850	T	C	7: 27,688,513 (GRCm39)	Y565C	probably damaging	Het
Zfp957	A	G	14: 79,450,207 (GRCm39)	Y531H	probably damaging	Het

Other mutations in Trps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00420:Trps1	APN	15	50,710,266 (GRCm39)	missense	probably benign	0.07
IGL00497:Trps1	APN	15	50,524,703 (GRCm39)	missense	possibly damaging	0.91
IGL00558:Trps1	APN	15	50,524,481 (GRCm39)	missense	probably damaging	1.00
IGL01325:Trps1	APN	15	50,710,210 (GRCm39)	missense	probably benign	0.40
IGL02132:Trps1	APN	15	50,685,674 (GRCm39)	missense	probably damaging	1.00
IGL02631:Trps1	APN	15	50,709,417 (GRCm39)	missense	probably damaging	1.00
IGL02740:Trps1	APN	15	50,709,935 (GRCm39)	missense	probably damaging	1.00
IGL02821:Trps1	APN	15	50,524,273 (GRCm39)	missense	probably damaging	1.00
IGL03096:Trps1	APN	15	50,709,875 (GRCm39)	missense	probably benign
F5770:Trps1	UTSW	15	50,694,973 (GRCm39)	missense	probably damaging	1.00
R0050:Trps1	UTSW	15	50,628,921 (GRCm39)	missense	probably benign	0.18
R0244:Trps1	UTSW	15	50,528,139 (GRCm39)	missense	probably damaging	1.00
R0377:Trps1	UTSW	15	50,695,174 (GRCm39)	nonsense	probably null
R0599:Trps1	UTSW	15	50,695,256 (GRCm39)	nonsense	probably null
R0848:Trps1	UTSW	15	50,524,945 (GRCm39)	missense	possibly damaging	0.54
R1744:Trps1	UTSW	15	50,524,609 (GRCm39)	missense	probably damaging	1.00
R1830:Trps1	UTSW	15	50,524,532 (GRCm39)	missense	probably damaging	0.99
R2083:Trps1	UTSW	15	50,685,701 (GRCm39)	missense	probably damaging	1.00
R2167:Trps1	UTSW	15	50,695,126 (GRCm39)	missense	possibly damaging	0.94
R2267:Trps1	UTSW	15	50,685,794 (GRCm39)	missense	probably damaging	1.00
R2314:Trps1	UTSW	15	50,524,742 (GRCm39)	missense	probably damaging	1.00
R3735:Trps1	UTSW	15	50,709,456 (GRCm39)	missense	possibly damaging	0.94
R4133:Trps1	UTSW	15	50,694,783 (GRCm39)	missense	probably damaging	1.00
R4223:Trps1	UTSW	15	50,710,044 (GRCm39)	missense	probably benign
R4280:Trps1	UTSW	15	50,709,478 (GRCm39)	missense	probably benign	0.00
R4566:Trps1	UTSW	15	50,695,074 (GRCm39)	missense	probably damaging	1.00
R4810:Trps1	UTSW	15	50,685,692 (GRCm39)	missense	probably benign	0.14
R4828:Trps1	UTSW	15	50,524,073 (GRCm39)	makesense	probably null
R4838:Trps1	UTSW	15	50,690,712 (GRCm39)	missense	probably benign	0.05
R4852:Trps1	UTSW	15	50,709,705 (GRCm39)	missense	probably damaging	1.00
R5001:Trps1	UTSW	15	50,524,703 (GRCm39)	missense	possibly damaging	0.91
R5311:Trps1	UTSW	15	50,528,156 (GRCm39)	missense	probably damaging	1.00
R5463:Trps1	UTSW	15	50,695,286 (GRCm39)	nonsense	probably null
R5691:Trps1	UTSW	15	50,690,700 (GRCm39)	missense	probably benign
R6432:Trps1	UTSW	15	50,694,793 (GRCm39)	missense	probably damaging	0.96
R6528:Trps1	UTSW	15	50,685,823 (GRCm39)	missense	probably benign	0.01
R6594:Trps1	UTSW	15	50,694,351 (GRCm39)	missense	probably damaging	0.99
R6827:Trps1	UTSW	15	50,685,959 (GRCm39)	missense	probably benign	0.14
R6862:Trps1	UTSW	15	50,695,001 (GRCm39)	critical splice donor site	probably null
R6912:Trps1	UTSW	15	50,685,694 (GRCm39)	missense	possibly damaging	0.92
R7151:Trps1	UTSW	15	50,685,793 (GRCm39)	missense	possibly damaging	0.95
R7846:Trps1	UTSW	15	50,695,273 (GRCm39)	missense	probably damaging	0.99
R7857:Trps1	UTSW	15	50,524,401 (GRCm39)	missense	probably damaging	1.00
R7986:Trps1	UTSW	15	50,753,019 (GRCm39)	missense	probably benign	0.00
R7986:Trps1	UTSW	15	50,525,132 (GRCm39)	missense	probably damaging	1.00
R8744:Trps1	UTSW	15	50,524,642 (GRCm39)	missense	probably damaging	1.00
R8838:Trps1	UTSW	15	50,753,007 (GRCm39)	missense	probably benign	0.01
R8859:Trps1	UTSW	15	50,685,769 (GRCm39)	missense	possibly damaging	0.77
R8935:Trps1	UTSW	15	50,752,344 (GRCm39)	nonsense	probably null
R9044:Trps1	UTSW	15	50,686,003 (GRCm39)	missense	probably benign	0.11
R9142:Trps1	UTSW	15	50,524,658 (GRCm39)	missense	probably damaging	0.98
R9211:Trps1	UTSW	15	50,694,840 (GRCm39)	missense	probably damaging	1.00
R9283:Trps1	UTSW	15	50,694,447 (GRCm39)	missense	probably damaging	1.00
R9363:Trps1	UTSW	15	50,524,676 (GRCm39)	missense	probably damaging	1.00
R9402:Trps1	UTSW	15	50,709,652 (GRCm39)	missense	probably damaging	1.00
R9562:Trps1	UTSW	15	50,524,657 (GRCm39)	missense	probably damaging	1.00
R9647:Trps1	UTSW	15	50,524,944 (GRCm39)	missense	probably benign	0.09
R9803:Trps1	UTSW	15	50,710,090 (GRCm39)	missense	possibly damaging	0.94
V7580:Trps1	UTSW	15	50,694,973 (GRCm39)	missense	probably damaging	1.00
V7581:Trps1	UTSW	15	50,694,973 (GRCm39)	missense	probably damaging	1.00
V7583:Trps1	UTSW	15	50,694,973 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCCTAAAAGCTATGTGTCTGG -3'
(R):5'- TCTGTCTCCAGAGCTTCAGGAC -3'

Sequencing Primer
(F):5'- CTTGGGAGCTGACACTGAAC -3'
(R):5'- CTCCAGAGCTTCAGGACTTTAAATGC -3'

Posted On

2016-11-09