Mutagenetix > Incidental Mutation

Incidental Mutation 'R5659:Usp32'

443998

Institutional Source

Beutler Lab

Gene Symbol

Usp32

Ensembl Gene

ENSMUSG00000000804

Gene Name

ubiquitin specific peptidase 32

Synonyms

2900074J03Rik, 6430526O11Rik

MMRRC Submission

043303-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5659 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

84875268-85030987 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 84968240 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 141 (V141A)

Ref Sequence

ENSEMBL: ENSMUSP00000103710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108075]

AlphaFold

F8VPZ3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000108075
AA Change: V141A

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000103710
Gene: ENSMUSG00000000804
AA Change: V141A

Domain	Start	End	E-Value	Type
EFh	232	260	4.66e0	SMART
EFh	268	296	5.8e-1	SMART
Blast:EFh	318	346	5e-7	BLAST
DUSP	389	588	2.32e-16	SMART
Pfam:Ubiquitin_3	628	711	2.4e-9	PFAM
Pfam:UCH	733	1564	2.4e-83	PFAM
Pfam:UCH_1	1202	1547	2.9e-12	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000121509

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173271

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.5%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankhd1	T	C	18: 36,694,103 (GRCm39)	S105P	probably damaging	Het
Ano5	G	A	7: 51,233,562 (GRCm39)	R658H	possibly damaging	Het
Ap3b2	T	A	7: 81,126,500 (GRCm39)	I367F	probably damaging	Het
Apaf1	A	T	10: 90,898,015 (GRCm39)	C247*	probably null	Het
Aqp8	G	A	7: 123,065,889 (GRCm39)	W228*	probably null	Het
Arhgap32	T	A	9: 32,093,256 (GRCm39)	V178D	probably damaging	Het
Atp10b	T	A	11: 43,136,252 (GRCm39)	W1127R	probably damaging	Het
Bcl6	A	T	16: 23,787,159 (GRCm39)	C580*	probably null	Het
Brd1	T	C	15: 88,597,584 (GRCm39)	T568A	probably benign	Het
Brsk1	C	T	7: 4,718,371 (GRCm39)	P665L	possibly damaging	Het
Cblc	A	G	7: 19,526,857 (GRCm39)	L125P	probably damaging	Het
Ccdc87	T	C	19: 4,890,878 (GRCm39)	S457P	probably damaging	Het
Cxcr5	C	T	9: 44,424,690 (GRCm39)	M322I	probably benign	Het
Cyb5r4	T	A	9: 86,937,881 (GRCm39)	F300Y	probably benign	Het
Cyp3a25	T	C	5: 145,928,356 (GRCm39)	T230A	possibly damaging	Het
Dhx9	A	G	1: 153,347,481 (GRCm39)	V409A	probably damaging	Het
Dnah7b	A	G	1: 46,392,009 (GRCm39)	D3790G	probably damaging	Het
Gin1	A	G	1: 97,703,257 (GRCm39)	T27A	possibly damaging	Het
Gipc1	A	T	8: 84,390,755 (GRCm39)	M287L	probably benign	Het
Kat6a	T	A	8: 23,428,176 (GRCm39)	L1177*	probably null	Het
Klhl20	A	G	1: 160,918,040 (GRCm39)	V82A	probably damaging	Het
Kmt2e	T	C	5: 23,702,805 (GRCm39)	I995T	probably damaging	Het
Lpin1	A	T	12: 16,590,990 (GRCm39)	V814E	probably damaging	Het
Luzp1	T	A	4: 136,269,787 (GRCm39)	V670D	probably damaging	Het
Lyst	C	A	13: 13,809,212 (GRCm39)	A294E	possibly damaging	Het
Olr1	T	A	6: 129,476,992 (GRCm39)	E91V	probably damaging	Het
Or1j1	A	T	2: 36,702,966 (GRCm39)	I46N	probably damaging	Het
Or2k2	A	T	4: 58,785,672 (GRCm39)	F17I	probably damaging	Het
Or8b55	C	T	9: 38,727,072 (GRCm39)	T91I	probably benign	Het
Pam	T	C	1: 97,770,024 (GRCm39)	Y476C	probably damaging	Het
Pcdhac1	T	C	18: 37,225,470 (GRCm39)	L761P	probably damaging	Het
Phf21b	C	T	15: 84,678,101 (GRCm39)	W300*	probably null	Het
Pld2	T	C	11: 70,448,387 (GRCm39)	*945Q	probably null	Het
Ppp1r37	C	T	7: 19,269,448 (GRCm39)	V145M	probably damaging	Het
Rasgrf1	T	A	9: 89,866,342 (GRCm39)	N593K	probably damaging	Het
Rhot1	T	G	11: 80,141,181 (GRCm39)		probably null	Het
Rmnd1	A	T	10: 4,377,382 (GRCm39)	M99K	probably benign	Het
Ros1	G	A	10: 52,019,482 (GRCm39)	T697I	possibly damaging	Het
Scgb1b10	G	T	7: 31,800,303 (GRCm39)	A4S	probably benign	Het
Shc3	T	C	13: 51,670,630 (GRCm39)	Y39C	probably damaging	Het
Slc25a23	A	G	17: 57,352,500 (GRCm39)		probably benign	Het
Slc5a8	C	T	10: 88,755,290 (GRCm39)	L466F	possibly damaging	Het
Sqor	G	A	2: 122,629,523 (GRCm39)	C127Y	probably benign	Het
Sv2a	G	C	3: 96,097,619 (GRCm39)	W467S	possibly damaging	Het
Togaram2	G	T	17: 71,994,667 (GRCm39)	D39Y	probably damaging	Het
Tspan11	T	A	6: 127,915,240 (GRCm39)		probably null	Het
Zbtb38	T	C	9: 96,569,473 (GRCm39)	H537R	probably damaging	Het
Zfat	T	C	15: 67,990,862 (GRCm39)	Y1008C	probably damaging	Het
Zfp637	G	A	6: 117,820,291 (GRCm39)	G3E	probably damaging	Het
Zfp788	T	A	7: 41,299,540 (GRCm39)	Y673*	probably null	Het
Zhx2	T	C	15: 57,685,704 (GRCm39)	S358P	probably benign	Het

Other mutations in Usp32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00529:Usp32	APN	11	84,885,252 (GRCm39)	missense	probably damaging	1.00
IGL00701:Usp32	APN	11	84,949,951 (GRCm39)	splice site	probably null
IGL00848:Usp32	APN	11	84,942,007 (GRCm39)	splice site	probably benign
IGL00934:Usp32	APN	11	84,897,902 (GRCm39)	missense	probably damaging	1.00
IGL01019:Usp32	APN	11	84,930,091 (GRCm39)	missense	probably damaging	0.97
IGL01302:Usp32	APN	11	84,879,308 (GRCm39)	missense	probably benign	0.05
IGL01444:Usp32	APN	11	84,949,990 (GRCm39)	missense	probably damaging	0.97
IGL01575:Usp32	APN	11	84,913,628 (GRCm39)	missense	probably damaging	1.00
IGL01981:Usp32	APN	11	84,927,350 (GRCm39)	missense	probably benign	0.02
IGL02118:Usp32	APN	11	84,923,003 (GRCm39)	nonsense	probably null
IGL02159:Usp32	APN	11	84,896,628 (GRCm39)	splice site	probably null
IGL02227:Usp32	APN	11	84,877,307 (GRCm39)	missense	probably damaging	1.00
IGL02363:Usp32	APN	11	84,935,613 (GRCm39)	missense	probably benign	0.01
IGL02524:Usp32	APN	11	84,900,837 (GRCm39)	nonsense	probably null
IGL02613:Usp32	APN	11	84,930,896 (GRCm39)	missense	probably damaging	0.99
IGL02720:Usp32	APN	11	84,897,817 (GRCm39)	critical splice donor site	probably null
IGL02738:Usp32	APN	11	84,974,632 (GRCm39)	missense	probably damaging	1.00
IGL02929:Usp32	APN	11	84,879,198 (GRCm39)	missense	probably benign	0.01
IGL03303:Usp32	APN	11	84,913,658 (GRCm39)	missense	probably damaging	1.00
BB010:Usp32	UTSW	11	84,897,885 (GRCm39)	missense	probably damaging	1.00
BB020:Usp32	UTSW	11	84,897,885 (GRCm39)	missense	probably damaging	1.00
PIT4812001:Usp32	UTSW	11	84,900,900 (GRCm39)	missense	probably damaging	1.00
R0026:Usp32	UTSW	11	84,922,900 (GRCm39)	missense	possibly damaging	0.48
R0295:Usp32	UTSW	11	84,944,518 (GRCm39)	missense	probably damaging	0.98
R1320:Usp32	UTSW	11	84,908,619 (GRCm39)	missense	probably damaging	0.98
R1712:Usp32	UTSW	11	84,933,406 (GRCm39)	missense	probably benign	0.12
R1922:Usp32	UTSW	11	84,897,830 (GRCm39)	nonsense	probably null
R1973:Usp32	UTSW	11	84,994,757 (GRCm39)	missense	probably benign	0.09
R2010:Usp32	UTSW	11	84,930,830 (GRCm39)	missense	probably damaging	0.98
R2082:Usp32	UTSW	11	84,921,338 (GRCm39)	missense	probably damaging	0.99
R2355:Usp32	UTSW	11	84,896,735 (GRCm39)	missense	probably benign	0.34
R3147:Usp32	UTSW	11	84,919,913 (GRCm39)	missense	probably damaging	1.00
R3160:Usp32	UTSW	11	84,916,362 (GRCm39)	missense	probably damaging	0.97
R3162:Usp32	UTSW	11	84,916,362 (GRCm39)	missense	probably damaging	0.97
R3716:Usp32	UTSW	11	84,933,389 (GRCm39)	missense	probably damaging	1.00
R3816:Usp32	UTSW	11	84,885,210 (GRCm39)	critical splice donor site	probably null
R3870:Usp32	UTSW	11	84,897,881 (GRCm39)	nonsense	probably null
R3871:Usp32	UTSW	11	84,971,982 (GRCm39)	missense	probably null	0.81
R4041:Usp32	UTSW	11	84,908,565 (GRCm39)	missense	probably benign	0.40
R4079:Usp32	UTSW	11	84,930,055 (GRCm39)	missense	probably damaging	0.98
R4332:Usp32	UTSW	11	84,994,804 (GRCm39)	missense	possibly damaging	0.79
R4396:Usp32	UTSW	11	84,944,801 (GRCm39)	missense	probably benign
R4580:Usp32	UTSW	11	84,949,953 (GRCm39)	critical splice donor site	probably null
R4620:Usp32	UTSW	11	84,949,953 (GRCm39)	critical splice donor site	probably null
R4744:Usp32	UTSW	11	84,885,219 (GRCm39)	missense	probably damaging	1.00
R4909:Usp32	UTSW	11	84,946,598 (GRCm39)	nonsense	probably null
R5056:Usp32	UTSW	11	84,917,621 (GRCm39)	missense	probably benign	0.07
R5111:Usp32	UTSW	11	84,968,157 (GRCm39)	missense	possibly damaging	0.95
R5213:Usp32	UTSW	11	84,913,085 (GRCm39)	missense	probably damaging	1.00
R5308:Usp32	UTSW	11	84,908,544 (GRCm39)	missense	probably benign	0.12
R5381:Usp32	UTSW	11	84,949,953 (GRCm39)	critical splice donor site	probably benign
R5538:Usp32	UTSW	11	84,908,612 (GRCm39)	missense	possibly damaging	0.65
R6006:Usp32	UTSW	11	84,883,277 (GRCm39)	critical splice donor site	probably null
R6011:Usp32	UTSW	11	84,922,923 (GRCm39)	missense	possibly damaging	0.70
R6029:Usp32	UTSW	11	84,916,408 (GRCm39)	missense	probably damaging	0.99
R6074:Usp32	UTSW	11	84,885,399 (GRCm39)	missense	probably benign	0.00
R6331:Usp32	UTSW	11	84,877,402 (GRCm39)	missense	possibly damaging	0.92
R6353:Usp32	UTSW	11	84,913,107 (GRCm39)	missense	probably benign
R6714:Usp32	UTSW	11	84,917,696 (GRCm39)	missense	probably damaging	0.99
R6778:Usp32	UTSW	11	84,916,512 (GRCm39)	missense	probably benign	0.00
R6988:Usp32	UTSW	11	84,900,969 (GRCm39)	missense	probably benign	0.35
R6992:Usp32	UTSW	11	84,922,914 (GRCm39)	missense	probably damaging	0.99
R7182:Usp32	UTSW	11	84,930,996 (GRCm39)	missense	probably benign	0.34
R7186:Usp32	UTSW	11	84,942,060 (GRCm39)	missense	probably benign	0.45
R7198:Usp32	UTSW	11	84,913,681 (GRCm39)	frame shift	probably null
R7201:Usp32	UTSW	11	84,913,681 (GRCm39)	frame shift	probably null
R7469:Usp32	UTSW	11	84,879,379 (GRCm39)	missense	possibly damaging	0.94
R7502:Usp32	UTSW	11	84,913,724 (GRCm39)	missense	possibly damaging	0.48
R7513:Usp32	UTSW	11	84,917,938 (GRCm39)	nonsense	probably null
R7629:Usp32	UTSW	11	84,910,681 (GRCm39)	frame shift	probably null
R7703:Usp32	UTSW	11	84,968,153 (GRCm39)	missense	probably damaging	0.99
R7741:Usp32	UTSW	11	84,878,107 (GRCm39)	missense	probably damaging	0.99
R7765:Usp32	UTSW	11	84,885,234 (GRCm39)	missense	probably damaging	1.00
R7933:Usp32	UTSW	11	84,897,885 (GRCm39)	missense	probably damaging	1.00
R7973:Usp32	UTSW	11	84,913,634 (GRCm39)	missense	probably damaging	0.99
R7989:Usp32	UTSW	11	84,925,126 (GRCm39)	missense
R7998:Usp32	UTSW	11	84,885,252 (GRCm39)	missense	probably damaging	1.00
R8292:Usp32	UTSW	11	84,968,227 (GRCm39)	missense	probably damaging	0.99
R8305:Usp32	UTSW	11	84,923,011 (GRCm39)	missense	possibly damaging	0.83
R8548:Usp32	UTSW	11	84,908,653 (GRCm39)	missense	possibly damaging	0.52
R8924:Usp32	UTSW	11	84,916,370 (GRCm39)	missense	probably damaging	0.98
R9002:Usp32	UTSW	11	84,944,777 (GRCm39)	missense	probably damaging	0.96
R9145:Usp32	UTSW	11	84,913,118 (GRCm39)	missense	probably damaging	1.00
R9209:Usp32	UTSW	11	84,930,838 (GRCm39)	missense	probably damaging	0.98
R9211:Usp32	UTSW	11	84,913,559 (GRCm39)	missense	probably damaging	1.00
R9296:Usp32	UTSW	11	84,908,478 (GRCm39)	missense	probably damaging	1.00
R9310:Usp32	UTSW	11	84,942,028 (GRCm39)	missense	probably benign	0.29
R9417:Usp32	UTSW	11	84,885,369 (GRCm39)	missense	probably damaging	1.00
R9514:Usp32	UTSW	11	84,913,560 (GRCm39)	missense	probably damaging	0.99
R9652:Usp32	UTSW	11	84,921,317 (GRCm39)	missense	probably damaging	0.97
R9723:Usp32	UTSW	11	84,935,536 (GRCm39)	nonsense	probably null
R9757:Usp32	UTSW	11	84,968,155 (GRCm39)	nonsense	probably null
X0028:Usp32	UTSW	11	84,883,432 (GRCm39)	missense	probably benign	0.05
Z1177:Usp32	UTSW	11	84,879,438 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCCAGCATCTCACTGATTATTTACTGG -3'
(R):5'- GGCAATTGACTGCTTTAACAAATTG -3'

Sequencing Primer
(F):5'- TCAGGCAAATTATTAAAGGTGAACG -3'
(R):5'- CTGAAATGCAGCTGTTTTGTTTTTC -3'

Posted On

2016-11-09