Incidental Mutation 'R5661:Gpat3'
ID444081
Institutional Source Beutler Lab
Gene Symbol Gpat3
Ensembl Gene ENSMUSG00000029314
Gene Nameglycerol-3-phosphate acyltransferase 3
SynonymsAgpat9, 4933407I02Rik, A230097K15Rik
MMRRC Submission 043304-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.198) question?
Stock #R5661 (G1)
Quality Score225
Status Validated
Chromosome5
Chromosomal Location100845713-100899102 bp(+) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) A to T at 100885942 bp
ZygosityHeterozygous
Amino Acid Change Lysine to Stop codon at position 221 (K221*)
Ref Sequence ENSEMBL: ENSMUSP00000108508 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000031255] [ENSMUST00000092990] [ENSMUST00000112887]
Predicted Effect probably null
Transcript: ENSMUST00000031255
AA Change: K221*
SMART Domains Protein: ENSMUSP00000031255
Gene: ENSMUSG00000029314
AA Change: K221*

DomainStartEndE-ValueType
transmembrane domain 10 32 N/A INTRINSIC
Blast:PlsC 99 160 7e-6 BLAST
low complexity region 166 177 N/A INTRINSIC
PlsC 223 334 5.96e-22 SMART
Blast:PlsC 348 396 2e-22 BLAST
Predicted Effect probably null
Transcript: ENSMUST00000092990
AA Change: K221*
SMART Domains Protein: ENSMUSP00000090667
Gene: ENSMUSG00000029314
AA Change: K221*

DomainStartEndE-ValueType
transmembrane domain 10 32 N/A INTRINSIC
Blast:PlsC 99 160 7e-6 BLAST
low complexity region 166 177 N/A INTRINSIC
PlsC 223 334 5.96e-22 SMART
Blast:PlsC 348 396 2e-22 BLAST
Predicted Effect probably null
Transcript: ENSMUST00000112887
AA Change: K221*
SMART Domains Protein: ENSMUSP00000108508
Gene: ENSMUSG00000029314
AA Change: K221*

DomainStartEndE-ValueType
transmembrane domain 10 32 N/A INTRINSIC
Blast:PlsC 99 160 7e-6 BLAST
low complexity region 166 177 N/A INTRINSIC
PlsC 223 334 5.96e-22 SMART
Blast:PlsC 348 396 2e-22 BLAST
Predicted Effect noncoding transcript
Transcript: ENSMUST00000197115
Meta Mutation Damage Score 0.65 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.3%
  • 20x: 95.4%
Validation Efficiency 97% (57/59)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the lysophosphatidic acid acyltransferase protein family. The encoded protein is an enzyme which catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]
PHENOTYPE: Under high-fat feeding, mice homozygous for a knock-out allele exhibit increased energy expenditure, improved glucose homeostasis, enlarged livers, increased total serum cholesterol levels, altered liver cholesterol metabolism, and female-specific protection from diet-induced obesity. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Actg2 A G 6: 83,520,772 I166T probably damaging Het
Actn1 T C 12: 80,184,844 E273G probably benign Het
Arhgap15 G A 2: 44,322,727 R403H possibly damaging Het
Arhgef26 T C 3: 62,377,654 probably benign Het
Avl9 A T 6: 56,725,102 R81* probably null Het
Brd3 G A 2: 27,461,572 T223I possibly damaging Het
Cacna2d4 T C 6: 119,343,531 M890T probably benign Het
Carm1 A G 9: 21,586,999 D433G probably benign Het
Ccdc40 A G 11: 119,237,927 K427E probably benign Het
Ccdc80 T C 16: 45,127,445 Y929H probably damaging Het
Ccr9 A C 9: 123,780,099 Y282S probably benign Het
Det1 C T 7: 78,843,210 E349K probably damaging Het
Enpep T A 3: 129,276,757 N834Y probably damaging Het
Epha7 A T 4: 28,946,217 probably null Het
Fap A T 2: 62,536,963 probably benign Het
Foxn4 A G 5: 114,272,992 C23R probably benign Het
Gad1-ps T C 10: 99,445,039 noncoding transcript Het
Gli2 C A 1: 118,853,302 E238* probably null Het
Gm20939 C A 17: 94,875,779 H148N probably damaging Het
Gnl1 T C 17: 35,982,555 Y211H probably benign Het
Hjurp G C 1: 88,277,215 probably benign Het
Hnrnph1 A T 11: 50,384,680 Q415L probably benign Het
Kansl3 T C 1: 36,348,957 E383G possibly damaging Het
Kdm5b T C 1: 134,599,073 V311A probably benign Het
Lipk T A 19: 34,032,327 M215K probably benign Het
Madd A G 2: 91,154,433 probably null Het
Meltf A G 16: 31,881,926 E88G possibly damaging Het
Mis18bp1 A T 12: 65,148,852 S713T probably benign Het
Mocos T A 18: 24,665,995 probably null Het
Msto1 T A 3: 88,912,885 D88V possibly damaging Het
Myo5a A G 9: 75,167,206 Y799C probably benign Het
Nectin4 T A 1: 171,385,170 L357H probably damaging Het
Olfr1184 G A 2: 88,487,097 V122M probably damaging Het
Olfr791 A T 10: 129,526,749 H174L probably benign Het
Pax2 A G 19: 44,790,722 N179S probably damaging Het
Pcdhac2 C A 18: 37,145,446 T493K probably damaging Het
Pgk2 G T 17: 40,207,396 C380* probably null Het
Pi4k2b G A 5: 52,743,564 probably null Het
Plcb3 A G 19: 6,963,220 V416A probably damaging Het
Pom121l2 G A 13: 21,984,255 G899R possibly damaging Het
Ppp4r1 T C 17: 65,803,968 probably null Het
Prkdc G A 16: 15,810,770 E3460K possibly damaging Het
Psmb3 A G 11: 97,706,833 E75G possibly damaging Het
Retnlb C T 16: 48,818,066 T50I probably benign Het
Sec16a A G 2: 26,439,637 S789P probably benign Het
Sec24d C T 3: 123,343,085 T489I probably damaging Het
Sec24d T C 3: 123,343,142 M508T possibly damaging Het
Slc5a8 C T 10: 88,919,428 L466F possibly damaging Het
Terf1 T A 1: 15,819,664 V221E probably damaging Het
Trak1 A G 9: 121,443,637 N187S possibly damaging Het
Trappc11 T A 8: 47,512,607 D528V probably damaging Het
Vmn1r170 A G 7: 23,606,806 N211S possibly damaging Het
Zcchc11 A G 4: 108,513,187 D761G probably benign Het
Zfpm2 G A 15: 41,096,071 W50* probably null Het
Other mutations in Gpat3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00848:Gpat3 APN 5 100893144 missense probably benign 0.01
R1429:Gpat3 UTSW 5 100893087 missense probably damaging 0.99
R1539:Gpat3 UTSW 5 100883388 missense probably benign 0.00
R1830:Gpat3 UTSW 5 100893180 missense probably benign
R2030:Gpat3 UTSW 5 100897821 missense probably benign
R2440:Gpat3 UTSW 5 100857173 missense probably benign
R2444:Gpat3 UTSW 5 100857173 missense probably benign
R3039:Gpat3 UTSW 5 100897805 missense possibly damaging 0.75
R3813:Gpat3 UTSW 5 100891639 splice site probably benign
R3830:Gpat3 UTSW 5 100884386 missense probably benign 0.02
R4636:Gpat3 UTSW 5 100857173 missense probably benign
R4637:Gpat3 UTSW 5 100857173 missense probably benign
R4679:Gpat3 UTSW 5 100893456 missense probably damaging 1.00
R4792:Gpat3 UTSW 5 100857173 missense probably benign
R5229:Gpat3 UTSW 5 100883424 missense probably damaging 1.00
R6383:Gpat3 UTSW 5 100893144 missense probably benign 0.01
X0023:Gpat3 UTSW 5 100885960 missense possibly damaging 0.88
Predicted Primers PCR Primer
(F):5'- GAGAATGTCATAGTCCCTCACC -3'
(R):5'- CCTGCACAGCTATGTCTCAG -3'

Sequencing Primer
(F):5'- CCAACTCATCATACTACGTGCGAG -3'
(R):5'- TCTGAAGTCAGCTACAGTGC -3'
Posted On2016-11-09