Mutagenetix > Incidental Mutation

Incidental Mutation 'R5662:Lrrc34'

444120

Institutional Source

Beutler Lab

Gene Symbol

Lrrc34

Ensembl Gene

ENSMUSG00000027702

Gene Name

leucine rich repeat containing 34

Synonyms

Spata34, 1700007J06Rik

MMRRC Submission

043305-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R5662 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

30678416-30701967 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 30685473 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 292 (Y292C)

Ref Sequence

ENSEMBL: ENSMUSP00000029252 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029252]

AlphaFold

Q9DAM1

Predicted Effect

probably benign
Transcript: ENSMUST00000029252
AA Change: Y292C

PolyPhen 2 Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000029252
Gene: ENSMUSG00000027702
AA Change: Y292C

Domain	Start	End	E-Value	Type
LRR	73	100	2.23e2	SMART
LRR	101	128	6.92e-1	SMART
LRR	129	156	1.78e0	SMART
LRR	157	184	1.67e-2	SMART
Blast:LRR	216	242	2e-9	BLAST
LRR	244	271	2.57e-3	SMART
LRR	272	299	5.59e-4	SMART
LRR	301	328	4.16e0	SMART
LRR	329	356	1.66e2	SMART

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.5%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	T	A	7: 78,749,855 (GRCm39)	V1542D	possibly damaging	Het
Acsm4	T	G	7: 119,294,023 (GRCm39)	N131K	possibly damaging	Het
Ankrd54	A	G	15: 78,946,814 (GRCm39)	S62P	possibly damaging	Het
Ccdc186	A	G	19: 56,781,920 (GRCm39)	I753T	probably benign	Het
Cers2	C	T	3: 95,228,295 (GRCm39)	R112C	probably damaging	Het
Col6a4	C	G	9: 105,945,200 (GRCm39)	R971S	probably damaging	Het
Ctsf	T	C	19: 4,906,606 (GRCm39)	S178P	probably damaging	Het
Dennd4c	C	T	4: 86,713,525 (GRCm39)	T492I	probably benign	Het
Dhx8	A	G	11: 101,657,584 (GRCm39)	K1212E	possibly damaging	Het
Dnah8	A	G	17: 30,956,307 (GRCm39)	T2096A	probably damaging	Het
Ephb4	A	T	5: 137,370,457 (GRCm39)	I886F	probably damaging	Het
Eprs1	T	G	1: 185,126,622 (GRCm39)	N519K	possibly damaging	Het
Fam3c	T	C	6: 22,355,061 (GRCm39)		probably benign	Het
Galnt17	G	A	5: 131,114,844 (GRCm39)	R219C	probably damaging	Het
Gm10645	A	G	8: 83,892,486 (GRCm39)		probably benign	Het
Gpr132	G	A	12: 112,816,416 (GRCm39)	R137C	probably damaging	Het
Hydin	T	G	8: 111,307,341 (GRCm39)	S3907A	probably benign	Het
Ighv1-49	A	T	12: 115,019,027 (GRCm39)	V56E	probably damaging	Het
Igtp	A	G	11: 58,097,105 (GRCm39)	D92G	probably damaging	Het
Itga1	C	T	13: 115,122,707 (GRCm39)	V750I	probably benign	Het
Katnip	A	G	7: 125,441,875 (GRCm39)	R595G	probably benign	Het
Kif3c	T	A	12: 3,417,031 (GRCm39)	Y351N	probably damaging	Het
Klhdc1	T	A	12: 69,329,939 (GRCm39)	I356N	probably benign	Het
Ly75	G	A	2: 60,182,725 (GRCm39)	T526M	probably damaging	Het
Mroh4	A	T	15: 74,497,277 (GRCm39)	D181E	possibly damaging	Het
Nutm1	T	C	2: 112,079,645 (GRCm39)	N757D	probably benign	Het
Or1e25	T	C	11: 73,494,005 (GRCm39)	F200L	probably benign	Het
Or1m1	A	G	9: 18,666,896 (GRCm39)	F12L	probably damaging	Het
Or6c215	T	C	10: 129,638,176 (GRCm39)	T73A	possibly damaging	Het
Or9s18	T	C	13: 65,300,067 (GRCm39)	F10L	possibly damaging	Het
Paxbp1	G	A	16: 90,834,285 (GRCm39)	T167M	probably benign	Het
Pnpla7	A	G	2: 24,942,396 (GRCm39)	D1238G	probably damaging	Het
Pom121l2	T	C	13: 22,166,358 (GRCm39)	S210P	probably benign	Het
Ripor3	T	G	2: 167,835,476 (GRCm39)	N165T	probably benign	Het
Serpine3	T	G	14: 62,908,291 (GRCm39)	S106R	probably benign	Het
Slc25a11	G	A	11: 70,536,245 (GRCm39)	R158*	probably null	Het
Slc26a6	G	A	9: 108,736,538 (GRCm39)	V506M	possibly damaging	Het
Slc45a2	A	G	15: 11,022,169 (GRCm39)	K304E	probably benign	Het
Slc4a4	C	T	5: 89,176,103 (GRCm39)	L25F	probably damaging	Het
Smarcb1	T	C	10: 75,740,404 (GRCm39)	N267S	possibly damaging	Het
Spink6	C	A	18: 44,207,481 (GRCm39)	Q24K	possibly damaging	Het
Srebf2	T	C	15: 82,079,204 (GRCm39)	S811P	probably benign	Het
Ssc4d	A	G	5: 135,989,748 (GRCm39)	*587R	probably null	Het
Sult2a8	C	T	7: 14,161,765 (GRCm39)	R27H	probably benign	Het
Sun2	C	T	15: 79,623,069 (GRCm39)	A90T	probably benign	Het
Ubr7	T	C	12: 102,734,526 (GRCm39)	S267P	probably benign	Het
Wdr55	G	A	18: 36,893,448 (GRCm39)	V37M	possibly damaging	Het
Xdh	C	T	17: 74,248,110 (GRCm39)	G45S	probably damaging	Het
Zfp638	T	A	6: 83,920,111 (GRCm39)	S570T	probably damaging	Het

Other mutations in Lrrc34

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02502:Lrrc34	APN	3	30,699,394 (GRCm39)	missense	probably benign	0.12
IGL02738:Lrrc34	APN	3	30,685,441 (GRCm39)	missense	possibly damaging	0.82
IGL02985:Lrrc34	APN	3	30,690,444 (GRCm39)	missense	probably benign	0.32
IGL02999:Lrrc34	APN	3	30,688,782 (GRCm39)	missense	probably damaging	0.99
R0367:Lrrc34	UTSW	3	30,684,142 (GRCm39)	missense	probably benign	0.08
R0761:Lrrc34	UTSW	3	30,685,425 (GRCm39)	splice site	probably null
R1426:Lrrc34	UTSW	3	30,697,728 (GRCm39)	unclassified	probably benign
R1980:Lrrc34	UTSW	3	30,696,890 (GRCm39)	missense	probably benign	0.33
R2215:Lrrc34	UTSW	3	30,697,678 (GRCm39)	missense	probably benign	0.03
R2414:Lrrc34	UTSW	3	30,688,711 (GRCm39)	missense	probably benign	0.00
R4379:Lrrc34	UTSW	3	30,685,524 (GRCm39)	missense	probably damaging	1.00
R5214:Lrrc34	UTSW	3	30,690,397 (GRCm39)	nonsense	probably null
R5418:Lrrc34	UTSW	3	30,696,923 (GRCm39)	missense	possibly damaging	0.85
R6736:Lrrc34	UTSW	3	30,679,008 (GRCm39)	missense	probably benign	0.03
R6809:Lrrc34	UTSW	3	30,688,749 (GRCm39)	missense	possibly damaging	0.80
R6941:Lrrc34	UTSW	3	30,678,969 (GRCm39)	missense	probably benign	0.01
R7017:Lrrc34	UTSW	3	30,699,465 (GRCm39)	critical splice acceptor site	probably null
R7080:Lrrc34	UTSW	3	30,688,705 (GRCm39)	missense	probably damaging	0.96
R7139:Lrrc34	UTSW	3	30,679,036 (GRCm39)	missense	probably benign	0.22
R7191:Lrrc34	UTSW	3	30,679,027 (GRCm39)	missense	possibly damaging	0.61
R7398:Lrrc34	UTSW	3	30,697,491 (GRCm39)	missense	probably damaging	1.00
R7662:Lrrc34	UTSW	3	30,697,452 (GRCm39)	missense	probably benign	0.16
R7707:Lrrc34	UTSW	3	30,679,041 (GRCm39)	missense	probably benign	0.00
R7945:Lrrc34	UTSW	3	30,696,886 (GRCm39)	critical splice donor site	probably null
R8799:Lrrc34	UTSW	3	30,678,979 (GRCm39)	missense	probably benign	0.06
R9764:Lrrc34	UTSW	3	30,697,467 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAACTTCTTGTTAAGAGTTTGGGATAA -3'
(R):5'- TCATCCTTGGAAGTTGAGCAA -3'

Sequencing Primer
(F):5'- GAGGATCCAAGTTCTGTTCACAGC -3'
(R):5'- AACTCGTGATGGAATGGT -3'

Posted On

2016-11-09