Mutagenetix > Incidental Mutation

Incidental Mutation 'R5738:Tas2r136'

444622

Institutional Source

Beutler Lab

Gene Symbol

Tas2r136

Ensembl Gene

ENSMUSG00000053217

Gene Name

taste receptor, type 2, member 136

Synonyms

Tas2r36, mt2r52

MMRRC Submission

043350-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

R5738 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132754142-132755125 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132754707 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 140 (L140P)

Ref Sequence

ENSEMBL: ENSMUSP00000070791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065532]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000065532
AA Change: L140P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000070791
Gene: ENSMUSG00000053217
AA Change: L140P

Domain	Start	End	E-Value	Type
Pfam:TAS2R	23	317	8.4e-68	PFAM

Meta Mutation Damage Score

0.4776

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

98% (52/53)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] TAS2R44 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway (Conte et al., 2002 [PubMed 12584440]). For further information on TAS2Rs, see MIM 604791.[supplied by OMIM, Mar 2009]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,571,917 (GRCm39)	D4826V	probably damaging	Het
Acoxl	G	A	2: 127,719,686 (GRCm39)	C149Y	probably benign	Het
Adamts3	G	T	5: 89,856,527 (GRCm39)	H349N	probably damaging	Het
Ap2b1	A	G	11: 83,227,256 (GRCm39)		probably null	Het
Ap3m2	T	C	8: 23,293,877 (GRCm39)	S58G	possibly damaging	Het
Bhmt2	A	T	13: 93,799,798 (GRCm39)	W213R	probably benign	Het
Cacna1h	T	G	17: 25,606,023 (GRCm39)	D1092A	probably damaging	Het
Cbfb	A	C	8: 105,929,193 (GRCm39)	Q170P	probably damaging	Het
Ccdc73	A	C	2: 104,761,331 (GRCm39)	K110N	possibly damaging	Het
Cep350	C	A	1: 155,741,824 (GRCm39)	R2149L	probably damaging	Het
Cog2	A	G	8: 125,272,777 (GRCm39)	T525A	probably benign	Het
Dnah8	G	A	17: 30,967,542 (GRCm39)	D2585N	probably benign	Het
Fbxl5	A	G	5: 43,920,170 (GRCm39)	I251T	probably benign	Het
Fscn3	A	G	6: 28,430,030 (GRCm39)	K67E	possibly damaging	Het
Glmp	T	A	3: 88,233,445 (GRCm39)	N133K	probably benign	Het
Gpr179	T	C	11: 97,242,232 (GRCm39)	N204S	probably damaging	Het
Gtf2ird1	C	T	5: 134,412,672 (GRCm39)	R613Q	probably damaging	Het
Hepacam	A	G	9: 37,294,721 (GRCm39)	D285G	possibly damaging	Het
Hipk4	G	A	7: 27,227,841 (GRCm39)	V196M	probably damaging	Het
Hlx	A	T	1: 184,463,754 (GRCm39)		probably null	Het
Igf2r	A	T	17: 12,936,254 (GRCm39)	D597E	probably benign	Het
Ighm	T	C	12: 113,385,115 (GRCm39)	T282A	unknown	Het
Igsf9b	T	C	9: 27,239,826 (GRCm39)	C624R	probably damaging	Het
Ksr2	T	C	5: 117,886,864 (GRCm39)	V800A	probably damaging	Het
Lyn	A	T	4: 3,782,987 (GRCm39)	I386F	probably damaging	Het
Melk	A	G	4: 44,310,333 (GRCm39)	D102G	probably damaging	Het
Mettl1	G	T	10: 126,877,863 (GRCm39)	E4*	probably null	Het
Mybl2	C	T	2: 162,910,203 (GRCm39)	Q210*	probably null	Het
Naga	C	T	15: 82,219,054 (GRCm39)	W231*	probably null	Het
Or2y3	T	C	17: 38,393,347 (GRCm39)	Y174C	probably damaging	Het
Or4k2	C	T	14: 50,424,105 (GRCm39)	V190I	probably benign	Het
Or7g16	T	C	9: 18,727,125 (GRCm39)	N155S	possibly damaging	Het
Otud4	T	C	8: 80,400,090 (GRCm39)	S935P	probably benign	Het
P2rx7	C	T	5: 122,790,852 (GRCm39)	T63I	probably damaging	Het
Pga5	A	T	19: 10,647,024 (GRCm39)	N260K	probably benign	Het
Phka2	ACC	AC	X: 159,342,862 (GRCm39)		probably null	Het
Plch1	T	A	3: 63,681,076 (GRCm39)	R184W	probably damaging	Het
Ppm1b	T	C	17: 85,301,374 (GRCm39)	F85L	probably benign	Het
Prtg	T	A	9: 72,819,288 (GRCm39)	F1094I	probably benign	Het
Ralgds	G	A	2: 28,432,538 (GRCm39)		probably benign	Het
Rgs17	A	C	10: 5,783,140 (GRCm39)	V149G	probably damaging	Het
Rnf168	A	G	16: 32,101,192 (GRCm39)	E124G	probably damaging	Het
Sav1	T	C	12: 70,022,817 (GRCm39)	E245G	possibly damaging	Het
Slc25a19	T	C	11: 115,515,060 (GRCm39)	I33V	probably benign	Het
Sptbn1	T	C	11: 30,095,941 (GRCm39)	I318V	probably damaging	Het
Tbc1d9	A	G	8: 83,997,655 (GRCm39)	I1071V	probably benign	Het
Tecta	G	T	9: 42,284,474 (GRCm39)	N870K	possibly damaging	Het
Tmem230	G	A	2: 132,086,048 (GRCm39)	P38L	possibly damaging	Het
Trpa1	G	T	1: 14,946,174 (GRCm39)	H986N	probably damaging	Het
Wdr41	A	T	13: 95,114,996 (GRCm39)	I24L	possibly damaging	Het

Other mutations in Tas2r136

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00678:Tas2r136	APN	6	132,754,161 (GRCm39)	missense	probably damaging	1.00
IGL02414:Tas2r136	APN	6	132,754,494 (GRCm39)	missense	possibly damaging	0.87
IGL02662:Tas2r136	APN	6	132,754,671 (GRCm39)	missense	probably damaging	1.00
R0607:Tas2r136	UTSW	6	132,754,375 (GRCm39)	missense	probably benign	0.09
R0785:Tas2r136	UTSW	6	132,754,890 (GRCm39)	missense	probably benign
R1727:Tas2r136	UTSW	6	132,754,753 (GRCm39)	missense	possibly damaging	0.63
R2101:Tas2r136	UTSW	6	132,754,495 (GRCm39)	missense	probably benign	0.09
R2975:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R3080:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R3746:Tas2r136	UTSW	6	132,754,200 (GRCm39)	missense	probably damaging	0.97
R3749:Tas2r136	UTSW	6	132,754,200 (GRCm39)	missense	probably damaging	0.97
R3750:Tas2r136	UTSW	6	132,754,200 (GRCm39)	missense	probably damaging	0.97
R4362:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R4411:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R4413:Tas2r136	UTSW	6	132,754,972 (GRCm39)	missense	probably damaging	1.00
R4803:Tas2r136	UTSW	6	132,754,455 (GRCm39)	missense	probably damaging	1.00
R5283:Tas2r136	UTSW	6	132,754,374 (GRCm39)	missense	probably damaging	0.99
R5343:Tas2r136	UTSW	6	132,755,043 (GRCm39)	missense	probably benign	0.00
R5872:Tas2r136	UTSW	6	132,754,294 (GRCm39)	missense	possibly damaging	0.46
R6770:Tas2r136	UTSW	6	132,754,345 (GRCm39)	missense	probably benign	0.03
R7326:Tas2r136	UTSW	6	132,754,869 (GRCm39)	missense	possibly damaging	0.65
R7845:Tas2r136	UTSW	6	132,754,833 (GRCm39)	missense	probably benign	0.05
R7900:Tas2r136	UTSW	6	132,754,599 (GRCm39)	missense	possibly damaging	0.93
R8422:Tas2r136	UTSW	6	132,754,290 (GRCm39)	missense	probably damaging	0.96
R8783:Tas2r136	UTSW	6	132,754,612 (GRCm39)	missense	probably benign	0.08
R8899:Tas2r136	UTSW	6	132,754,323 (GRCm39)	missense	probably benign
R9126:Tas2r136	UTSW	6	132,754,584 (GRCm39)	missense	probably damaging	1.00
R9627:Tas2r136	UTSW	6	132,754,948 (GRCm39)	missense	possibly damaging	0.61
R9642:Tas2r136	UTSW	6	132,754,462 (GRCm39)	missense	probably benign	0.13
R9749:Tas2r136	UTSW	6	132,755,106 (GRCm39)	missense	probably damaging	1.00
R9765:Tas2r136	UTSW	6	132,754,813 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GGGAACTAAACTGCCTAGAGTG -3'
(R):5'- TCTGGCTATCTTCAGGTTTGAC -3'

Sequencing Primer
(F):5'- CTGCCTAGAGTGAAAGTCATAGC -3'
(R):5'- CTATCTTCAGGTTTGACTTGCTTTG -3'

Posted On

2016-11-21