Mutagenetix > Incidental Mutation

Incidental Mutation 'R5753:Vmn2r12'

444886

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r12

Ensembl Gene

ENSMUSG00000090688

Gene Name

vomeronasal 2, receptor 12

Synonyms

Gm6769

MMRRC Submission

043358-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R5753 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

109233715-109245730 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109239670 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 298 (W298R)

Ref Sequence

ENSEMBL: ENSMUSP00000093612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095922]

AlphaFold

L7N217

Predicted Effect

probably damaging
Transcript: ENSMUST00000095922
AA Change: W298R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000093612
Gene: ENSMUSG00000090688
AA Change: W298R

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.8e-30	PFAM
Pfam:NCD3G	505	559	1.7e-18	PFAM
Pfam:7tm_3	591	827	3.9e-54	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 96.0%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310003L06Rik	C	T	5: 88,120,374 (GRCm39)	T377I	probably damaging	Het
Abcb1a	A	G	5: 8,773,160 (GRCm39)	D796G	probably damaging	Het
Actn3	T	C	19: 4,914,595 (GRCm39)		probably null	Het
Ada	A	T	2: 163,577,318 (GRCm39)	S57T	probably benign	Het
Adamts6	A	G	13: 104,483,858 (GRCm39)	Y359C	probably damaging	Het
Adcy1	A	T	11: 7,080,300 (GRCm39)	I348F	probably damaging	Het
Ankrd11	T	C	8: 123,622,043 (GRCm39)	E603G	possibly damaging	Het
Bcas3	A	G	11: 85,712,910 (GRCm39)		probably benign	Het
Cldn1	C	A	16: 26,181,871 (GRCm39)	V113L	probably benign	Het
Dock9	T	C	14: 121,872,037 (GRCm39)	T540A	probably benign	Het
Erp27	A	G	6: 136,896,875 (GRCm39)	F109S	probably damaging	Het
F13a1	G	A	13: 37,082,082 (GRCm39)	Q541*	probably null	Het
Fam124a	T	C	14: 62,843,988 (GRCm39)	S499P	probably benign	Het
Fgd3	T	A	13: 49,428,416 (GRCm39)	E486V	possibly damaging	Het
Flnc	T	C	6: 29,433,488 (GRCm39)	S11P	probably benign	Het
Gm12508	C	A	4: 55,254,787 (GRCm39)		noncoding transcript	Het
Grk2	C	T	19: 4,340,496 (GRCm39)	R295H	probably damaging	Het
Gtf2ird1	A	G	5: 134,439,837 (GRCm39)	M131T	probably damaging	Het
H4c4	G	A	13: 23,765,587 (GRCm39)	M1I	probably null	Het
Itpripl2	A	G	7: 118,090,232 (GRCm39)	V109A	probably damaging	Het
Jakmip2	T	C	18: 43,692,181 (GRCm39)	E585G	probably damaging	Het
Kbtbd3	A	T	9: 4,331,404 (GRCm39)	I593F	possibly damaging	Het
Lgr4	C	T	2: 109,832,857 (GRCm39)	Q316*	probably null	Het
Mcf2l	T	C	8: 13,049,993 (GRCm39)	F305S	probably damaging	Het
Nos1ap	T	A	1: 170,176,968 (GRCm39)	K145M	probably damaging	Het
Or4c3	T	C	2: 89,851,847 (GRCm39)	T188A	possibly damaging	Het
Or4k51	T	A	2: 111,585,146 (GRCm39)	I184K	possibly damaging	Het
Or4q3	T	A	14: 50,583,045 (GRCm39)	T285S	probably damaging	Het
Or51b17	A	G	7: 103,542,408 (GRCm39)	L178P	probably damaging	Het
Or5d18	G	A	2: 87,864,920 (GRCm39)	L188F	probably damaging	Het
Or5w18	A	T	2: 87,633,596 (GRCm39)	M288L	probably benign	Het
Or7a40	A	T	16: 16,491,484 (GRCm39)	Y120*	probably null	Het
Pcdh9	T	A	14: 94,125,597 (GRCm39)	D191V	probably damaging	Het
Pde4d	T	A	13: 109,909,256 (GRCm39)		probably benign	Het
Phkb	C	T	8: 86,604,859 (GRCm39)	A88V	probably damaging	Het
Plec	A	G	15: 76,057,620 (GRCm39)	S4128P	probably damaging	Het
Plekha5	A	T	6: 140,482,730 (GRCm39)		probably null	Het
Rgs13	A	C	1: 144,016,478 (GRCm39)	N88K	probably benign	Het
Rho	T	A	6: 115,912,448 (GRCm39)	I104N	probably damaging	Het
Rnps1	T	C	17: 24,637,138 (GRCm39)		probably benign	Het
Saxo5	A	T	8: 3,534,112 (GRCm39)	I350L	probably benign	Het
Slc10a7	G	T	8: 79,251,928 (GRCm39)		probably null	Het
Slc39a7	C	T	17: 34,249,150 (GRCm39)	R246K	probably damaging	Het
Sort1	G	C	3: 108,253,090 (GRCm39)	G510A	probably damaging	Het
Spag6l	A	G	16: 16,584,831 (GRCm39)		probably null	Het
Sync	T	A	4: 129,187,179 (GRCm39)	Y70*	probably null	Het
Timm8a2	T	C	14: 122,272,289 (GRCm39)	V64A	probably benign	Het
Tnfrsf11b	C	A	15: 54,117,455 (GRCm39)	V267L	possibly damaging	Het
Trim30b	A	G	7: 104,006,544 (GRCm39)	V104A	possibly damaging	Het
Tusc3	T	C	8: 39,564,100 (GRCm39)	S244P	probably damaging	Het
Usp16	T	A	16: 87,279,787 (GRCm39)	Y746N	probably damaging	Het
Vax1	G	T	19: 59,154,814 (GRCm39)	H274Q	probably benign	Het
Vmn2r108	A	G	17: 20,683,179 (GRCm39)	V675A	probably damaging	Het
Zfp948	A	G	17: 21,807,156 (GRCm39)	N116S	probably damaging	Het
Zkscan2	A	G	7: 123,079,923 (GRCm39)	V678A	probably benign	Het

Other mutations in Vmn2r12

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00948:Vmn2r12	APN	5	109,245,541 (GRCm39)	missense	possibly damaging	0.47
IGL01096:Vmn2r12	APN	5	109,234,125 (GRCm39)	missense	probably damaging	1.00
IGL01538:Vmn2r12	APN	5	109,239,716 (GRCm39)	missense	probably damaging	1.00
IGL01548:Vmn2r12	APN	5	109,240,893 (GRCm39)	nonsense	probably null
IGL01762:Vmn2r12	APN	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
IGL01860:Vmn2r12	APN	5	109,240,025 (GRCm39)	missense	probably benign	0.10
IGL02269:Vmn2r12	APN	5	109,234,343 (GRCm39)	missense	probably damaging	1.00
IGL02530:Vmn2r12	APN	5	109,233,858 (GRCm39)	missense	probably damaging	1.00
IGL02887:Vmn2r12	APN	5	109,238,351 (GRCm39)	missense	probably benign	0.03
IGL03265:Vmn2r12	APN	5	109,239,936 (GRCm39)	missense	probably benign	0.05
R0396:Vmn2r12	UTSW	5	109,240,765 (GRCm39)	missense	probably benign	0.00
R0497:Vmn2r12	UTSW	5	109,239,755 (GRCm39)	nonsense	probably null
R0529:Vmn2r12	UTSW	5	109,240,714 (GRCm39)	missense	probably benign
R0715:Vmn2r12	UTSW	5	109,238,373 (GRCm39)	missense	probably benign	0.10
R0742:Vmn2r12	UTSW	5	109,234,281 (GRCm39)	missense	possibly damaging	0.55
R0894:Vmn2r12	UTSW	5	109,235,716 (GRCm39)	critical splice donor site	probably null
R1173:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1174:Vmn2r12	UTSW	5	109,240,720 (GRCm39)	missense	probably benign	0.00
R1259:Vmn2r12	UTSW	5	109,239,763 (GRCm39)	missense	probably damaging	0.97
R1349:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R1388:Vmn2r12	UTSW	5	109,240,840 (GRCm39)	missense	possibly damaging	0.56
R1549:Vmn2r12	UTSW	5	109,240,696 (GRCm39)	missense	probably benign	0.06
R1766:Vmn2r12	UTSW	5	109,239,910 (GRCm39)	missense	probably damaging	1.00
R1781:Vmn2r12	UTSW	5	109,239,594 (GRCm39)	missense	probably benign	0.00
R1885:Vmn2r12	UTSW	5	109,239,942 (GRCm39)	missense	probably damaging	1.00
R2159:Vmn2r12	UTSW	5	109,239,340 (GRCm39)	missense	probably benign	0.02
R2420:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2421:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2422:Vmn2r12	UTSW	5	109,234,398 (GRCm39)	missense	probably benign	0.39
R2937:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R2938:Vmn2r12	UTSW	5	109,239,397 (GRCm39)	missense	probably damaging	1.00
R3898:Vmn2r12	UTSW	5	109,238,370 (GRCm39)	missense	probably benign	0.02
R4061:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4063:Vmn2r12	UTSW	5	109,240,058 (GRCm39)	missense	possibly damaging	0.95
R4090:Vmn2r12	UTSW	5	109,239,412 (GRCm39)	missense	probably benign	0.06
R4297:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4298:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4299:Vmn2r12	UTSW	5	109,239,830 (GRCm39)	missense	probably benign	0.12
R4304:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4308:Vmn2r12	UTSW	5	109,233,872 (GRCm39)	missense	probably damaging	1.00
R4594:Vmn2r12	UTSW	5	109,234,301 (GRCm39)	missense	probably damaging	1.00
R4783:Vmn2r12	UTSW	5	109,234,379 (GRCm39)	missense	probably damaging	1.00
R4900:Vmn2r12	UTSW	5	109,240,852 (GRCm39)	missense	probably damaging	1.00
R4929:Vmn2r12	UTSW	5	109,239,544 (GRCm39)	missense	probably damaging	1.00
R4974:Vmn2r12	UTSW	5	109,239,372 (GRCm39)	missense	probably damaging	1.00
R5389:Vmn2r12	UTSW	5	109,238,261 (GRCm39)	missense	probably benign	0.00
R5431:Vmn2r12	UTSW	5	109,239,684 (GRCm39)	missense	probably damaging	0.99
R5527:Vmn2r12	UTSW	5	109,234,483 (GRCm39)	nonsense	probably null
R5639:Vmn2r12	UTSW	5	109,240,666 (GRCm39)	missense	probably benign	0.06
R5797:Vmn2r12	UTSW	5	109,233,736 (GRCm39)	nonsense	probably null
R6142:Vmn2r12	UTSW	5	109,240,763 (GRCm39)	missense	probably benign
R6162:Vmn2r12	UTSW	5	109,234,430 (GRCm39)	missense	probably damaging	0.99
R6176:Vmn2r12	UTSW	5	109,233,866 (GRCm39)	missense	probably benign	0.43
R6853:Vmn2r12	UTSW	5	109,240,771 (GRCm39)	missense	probably damaging	1.00
R7238:Vmn2r12	UTSW	5	109,245,655 (GRCm39)	missense	possibly damaging	0.81
R7341:Vmn2r12	UTSW	5	109,239,811 (GRCm39)	missense	possibly damaging	0.95
R7341:Vmn2r12	UTSW	5	109,234,113 (GRCm39)	missense	possibly damaging	0.74
R7383:Vmn2r12	UTSW	5	109,240,684 (GRCm39)	missense	probably benign	0.19
R7740:Vmn2r12	UTSW	5	109,239,615 (GRCm39)	missense	probably damaging	1.00
R7749:Vmn2r12	UTSW	5	109,233,920 (GRCm39)	missense	probably damaging	0.99
R7861:Vmn2r12	UTSW	5	109,235,829 (GRCm39)	missense	probably benign	0.00
R7908:Vmn2r12	UTSW	5	109,234,307 (GRCm39)	missense	probably damaging	1.00
R8128:Vmn2r12	UTSW	5	109,239,747 (GRCm39)	missense	possibly damaging	0.81
R8175:Vmn2r12	UTSW	5	109,238,349 (GRCm39)	missense	probably damaging	0.97
R8234:Vmn2r12	UTSW	5	109,234,074 (GRCm39)	missense	probably benign	0.01
R8771:Vmn2r12	UTSW	5	109,239,952 (GRCm39)	missense	possibly damaging	0.95
R8947:Vmn2r12	UTSW	5	109,234,522 (GRCm39)	missense	possibly damaging	0.64
R8991:Vmn2r12	UTSW	5	109,234,033 (GRCm39)	nonsense	probably null
R9116:Vmn2r12	UTSW	5	109,233,885 (GRCm39)	missense	probably damaging	1.00
R9122:Vmn2r12	UTSW	5	109,240,910 (GRCm39)	missense	probably benign	0.00
R9153:Vmn2r12	UTSW	5	109,234,203 (GRCm39)	missense	probably damaging	1.00
R9371:Vmn2r12	UTSW	5	109,234,452 (GRCm39)	missense	probably benign	0.00
R9375:Vmn2r12	UTSW	5	109,233,986 (GRCm39)	missense	probably damaging	1.00
R9524:Vmn2r12	UTSW	5	109,239,823 (GRCm39)	missense	probably damaging	1.00
R9587:Vmn2r12	UTSW	5	109,239,322 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r12	UTSW	5	109,240,646 (GRCm39)	missense	probably benign
Z1176:Vmn2r12	UTSW	5	109,239,303 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTGCTGTCCATTCCAATGTG -3'
(R):5'- TGATCCCAGAAGACATGCAG -3'

Sequencing Primer
(F):5'- CCATTCCAATGTGTTGTTGAATG -3'
(R):5'- CATGCAGTTATATATGACAAGGGCTG -3'

Posted On

2016-11-21