Incidental Mutation 'R5774:Il17a'
ID 445538
Institutional Source Beutler Lab
Gene Symbol Il17a
Ensembl Gene ENSMUSG00000025929
Gene Name interleukin 17A
Synonyms IL-17A, Il17, Ctla-8, Ctla8
MMRRC Submission 043373-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5774 (G1)
Quality Score 217
Status Not validated
Chromosome 1
Chromosomal Location 20801129-20804720 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 20803997 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Serine to Glycine at position 131 (S131G)
Ref Sequence ENSEMBL: ENSMUSP00000027061 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000027061]
AlphaFold Q62386
Predicted Effect probably benign
Transcript: ENSMUST00000027061
AA Change: S131G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
SMART Domains Protein: ENSMUSP00000027061
Gene: ENSMUSG00000025929
AA Change: S131G

DomainStartEndE-ValueType
signal peptide 1 25 N/A INTRINSIC
Pfam:IL17 72 150 3.9e-30 PFAM
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.7%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a pro-inflammatory cytokine that is a member of the interleukin-17 family. The encoded protein plays a central role in host defense against diverse pathogens. The encoded protein is produced by activated T-cells and certain cell types of innate immune system. The active protein functions as either a homodimer with other interleukin-17 family members and signals through the interleukin-17 receptor to induce inflammatory cytokine production. Aberrant expression of this gene is associated with autoinflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit reduced contact, delayed-type and airway hypersensitivity responses and impaired T-dependent antibody production. [provided by MGI curators]
Allele List at MGI

All alleles(7) : Targeted(7)
Other mutations in this stock
Total: 51 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
5730507C01Rik C A 12: 18,581,668 (GRCm39) L62M probably damaging Het
Abcc9 G A 6: 142,574,285 (GRCm39) T949I probably damaging Het
Adam4 C T 12: 81,467,460 (GRCm39) S387N probably damaging Het
Akap11 T C 14: 78,748,407 (GRCm39) S1327G probably damaging Het
Arhgap1 A G 2: 91,484,453 (GRCm39) T12A possibly damaging Het
Arhgap28 A G 17: 68,188,487 (GRCm39) S228P possibly damaging Het
Atp6v1b2 A T 8: 69,554,613 (GRCm39) D106V probably damaging Het
Atp9b T G 18: 80,977,147 (GRCm39) D3A probably damaging Het
Bptf A G 11: 107,001,963 (GRCm39) F383S probably damaging Het
Cdh24 T C 14: 54,876,514 (GRCm39) T104A probably damaging Het
Cep55 A G 19: 38,051,103 (GRCm39) E171G probably damaging Het
Cntrl T A 2: 35,052,873 (GRCm39) M1126K probably benign Het
Cts3 A T 13: 61,716,184 (GRCm39) I59N probably damaging Het
Ddx52 A G 11: 83,836,960 (GRCm39) I150M probably damaging Het
Dennd6a T C 14: 26,300,974 (GRCm39) V62A probably benign Het
Dpep1 C A 8: 123,926,721 (GRCm39) D211E probably damaging Het
Gm1527 T C 3: 28,972,239 (GRCm39) V452A probably benign Het
Hmcn2 T C 2: 31,299,147 (GRCm39) V2831A possibly damaging Het
Hrob T A 11: 102,146,495 (GRCm39) I257N possibly damaging Het
Hydin G T 8: 111,298,547 (GRCm39) E3722* probably null Het
Ing3 C T 6: 21,967,688 (GRCm39) P119S probably benign Het
Larp4b T A 13: 9,220,679 (GRCm39) probably null Het
Lca5l G T 16: 95,977,261 (GRCm39) Q177K probably benign Het
Lrrc61 C T 6: 48,545,133 (GRCm39) probably benign Het
Man2a2 T C 7: 80,018,106 (GRCm39) Y188C probably damaging Het
Mdk T C 2: 91,761,569 (GRCm39) E36G probably damaging Het
Mmp12 T C 9: 7,354,823 (GRCm39) I272T possibly damaging Het
Myh4 A T 11: 67,144,034 (GRCm39) K1135* probably null Het
Nup188 T A 2: 30,191,060 (GRCm39) Y96N probably damaging Het
Or8k33 A T 2: 86,384,351 (GRCm39) V39E possibly damaging Het
Pank4 G A 4: 155,065,119 (GRCm39) G806D probably damaging Het
Parp14 T C 16: 35,678,780 (GRCm39) Y396C probably damaging Het
Pcdhb8 T A 18: 37,489,738 (GRCm39) I472N probably damaging Het
Sema3a T A 5: 13,573,131 (GRCm39) W220R probably damaging Het
Slc35g3 A G 11: 69,651,124 (GRCm39) V309A probably damaging Het
Slc6a6 A G 6: 91,721,981 (GRCm39) M394V probably damaging Het
Specc1l G A 10: 75,081,234 (GRCm39) R210H probably damaging Het
Spocd1 T A 4: 129,845,579 (GRCm39) S480T probably benign Het
Sptbn5 T A 2: 119,880,939 (GRCm39) noncoding transcript Het
Srrm2 A G 17: 24,037,249 (GRCm39) probably benign Het
Stx16 G A 2: 173,935,292 (GRCm39) G156R probably damaging Het
Topbp1 A G 9: 103,205,698 (GRCm39) K779E probably benign Het
Trank1 T A 9: 111,220,294 (GRCm39) F2344I probably damaging Het
Ttll5 GCCCTGCGGGGCTGCCACGCTGTCGATCCGGCAGCTAC G 12: 85,980,329 (GRCm39) probably null Het
Ube4a G A 9: 44,864,395 (GRCm39) P66L probably damaging Het
Utp6 A T 11: 79,844,424 (GRCm39) F200L probably benign Het
Vmn2r117 A T 17: 23,696,176 (GRCm39) H410Q probably damaging Het
Vps33b C T 7: 79,935,088 (GRCm39) H344Y probably benign Het
Xpo5 C T 17: 46,552,772 (GRCm39) R1145* probably null Het
Zbed4 T A 15: 88,665,852 (GRCm39) F640Y possibly damaging Het
Zfp618 C T 4: 63,050,799 (GRCm39) R527C probably damaging Het
Other mutations in Il17a
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00496:Il17a APN 1 20,802,507 (GRCm39) missense probably damaging 1.00
IGL01767:Il17a APN 1 20,803,864 (GRCm39) missense probably benign 0.03
seventeenager UTSW 1 20,803,870 (GRCm39) nonsense probably null
R0057:Il17a UTSW 1 20,803,881 (GRCm39) missense probably damaging 1.00
R4597:Il17a UTSW 1 20,801,217 (GRCm39) splice site probably null
R5436:Il17a UTSW 1 20,803,870 (GRCm39) nonsense probably null
R5932:Il17a UTSW 1 20,803,977 (GRCm39) missense probably damaging 1.00
R6104:Il17a UTSW 1 20,802,498 (GRCm39) missense probably damaging 1.00
R7142:Il17a UTSW 1 20,802,327 (GRCm39) missense probably benign
R7838:Il17a UTSW 1 20,802,351 (GRCm39) missense probably benign
R7989:Il17a UTSW 1 20,802,438 (GRCm39) missense possibly damaging 0.89
Predicted Primers PCR Primer
(F):5'- AAGCAGGACTTCATTTCCTCC -3'
(R):5'- CTGAATCTGCCTCTGAATCCAC -3'

Sequencing Primer
(F):5'- GGACTTCATTTCCTCCTGGCTTTTG -3'
(R):5'- TCCTTGCTGAAAATCAATAGCACG -3'
Posted On 2016-11-21