Incidental Mutation 'R5774:Or8k33'
| ID |
445543 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Or8k33
|
| Ensembl Gene |
ENSMUSG00000110912 |
| Gene Name |
olfactory receptor family 8 subfamily K member 33 |
| Synonyms |
GA_x6K02T2Q125-48039418-48038477, MOR192-1, Olfr1080 |
| MMRRC Submission |
043373-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.087)
|
| Stock # |
R5774 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
86383525-86388482 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 86384351 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Glutamic Acid
at position 39
(V39E)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000150502
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000213185]
|
| AlphaFold |
Q7TR67 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000099881
AA Change: V39E
PolyPhen 2
Score 0.683 (Sensitivity: 0.86; Specificity: 0.92)
|
| SMART Domains |
Protein: ENSMUSP00000097466
Gene: ENSMUSG00000075178
AA Change: V39E
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_1
|
41 |
290 |
1.5e-30 |
PFAM |
|
Pfam:7tm_4
|
139 |
283 |
1.4e-44 |
PFAM |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000213185
AA Change: V39E
PolyPhen 2
Score 0.683 (Sensitivity: 0.86; Specificity: 0.92)
|
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.4%
- 20x: 95.7%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 51 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 5730507C01Rik |
C |
A |
12: 18,581,668 (GRCm39) |
L62M |
probably damaging |
Het |
| Abcc9 |
G |
A |
6: 142,574,285 (GRCm39) |
T949I |
probably damaging |
Het |
| Adam4 |
C |
T |
12: 81,467,460 (GRCm39) |
S387N |
probably damaging |
Het |
| Akap11 |
T |
C |
14: 78,748,407 (GRCm39) |
S1327G |
probably damaging |
Het |
| Arhgap1 |
A |
G |
2: 91,484,453 (GRCm39) |
T12A |
possibly damaging |
Het |
| Arhgap28 |
A |
G |
17: 68,188,487 (GRCm39) |
S228P |
possibly damaging |
Het |
| Atp6v1b2 |
A |
T |
8: 69,554,613 (GRCm39) |
D106V |
probably damaging |
Het |
| Atp9b |
T |
G |
18: 80,977,147 (GRCm39) |
D3A |
probably damaging |
Het |
| Bptf |
A |
G |
11: 107,001,963 (GRCm39) |
F383S |
probably damaging |
Het |
| Cdh24 |
T |
C |
14: 54,876,514 (GRCm39) |
T104A |
probably damaging |
Het |
| Cep55 |
A |
G |
19: 38,051,103 (GRCm39) |
E171G |
probably damaging |
Het |
| Cntrl |
T |
A |
2: 35,052,873 (GRCm39) |
M1126K |
probably benign |
Het |
| Cts3 |
A |
T |
13: 61,716,184 (GRCm39) |
I59N |
probably damaging |
Het |
| Ddx52 |
A |
G |
11: 83,836,960 (GRCm39) |
I150M |
probably damaging |
Het |
| Dennd6a |
T |
C |
14: 26,300,974 (GRCm39) |
V62A |
probably benign |
Het |
| Dpep1 |
C |
A |
8: 123,926,721 (GRCm39) |
D211E |
probably damaging |
Het |
| Gm1527 |
T |
C |
3: 28,972,239 (GRCm39) |
V452A |
probably benign |
Het |
| Hmcn2 |
T |
C |
2: 31,299,147 (GRCm39) |
V2831A |
possibly damaging |
Het |
| Hrob |
T |
A |
11: 102,146,495 (GRCm39) |
I257N |
possibly damaging |
Het |
| Hydin |
G |
T |
8: 111,298,547 (GRCm39) |
E3722* |
probably null |
Het |
| Il17a |
A |
G |
1: 20,803,997 (GRCm39) |
S131G |
probably benign |
Het |
| Ing3 |
C |
T |
6: 21,967,688 (GRCm39) |
P119S |
probably benign |
Het |
| Larp4b |
T |
A |
13: 9,220,679 (GRCm39) |
|
probably null |
Het |
| Lca5l |
G |
T |
16: 95,977,261 (GRCm39) |
Q177K |
probably benign |
Het |
| Lrrc61 |
C |
T |
6: 48,545,133 (GRCm39) |
|
probably benign |
Het |
| Man2a2 |
T |
C |
7: 80,018,106 (GRCm39) |
Y188C |
probably damaging |
Het |
| Mdk |
T |
C |
2: 91,761,569 (GRCm39) |
E36G |
probably damaging |
Het |
| Mmp12 |
T |
C |
9: 7,354,823 (GRCm39) |
I272T |
possibly damaging |
Het |
| Myh4 |
A |
T |
11: 67,144,034 (GRCm39) |
K1135* |
probably null |
Het |
| Nup188 |
T |
A |
2: 30,191,060 (GRCm39) |
Y96N |
probably damaging |
Het |
| Pank4 |
G |
A |
4: 155,065,119 (GRCm39) |
G806D |
probably damaging |
Het |
| Parp14 |
T |
C |
16: 35,678,780 (GRCm39) |
Y396C |
probably damaging |
Het |
| Pcdhb8 |
T |
A |
18: 37,489,738 (GRCm39) |
I472N |
probably damaging |
Het |
| Sema3a |
T |
A |
5: 13,573,131 (GRCm39) |
W220R |
probably damaging |
Het |
| Slc35g3 |
A |
G |
11: 69,651,124 (GRCm39) |
V309A |
probably damaging |
Het |
| Slc6a6 |
A |
G |
6: 91,721,981 (GRCm39) |
M394V |
probably damaging |
Het |
| Specc1l |
G |
A |
10: 75,081,234 (GRCm39) |
R210H |
probably damaging |
Het |
| Spocd1 |
T |
A |
4: 129,845,579 (GRCm39) |
S480T |
probably benign |
Het |
| Sptbn5 |
T |
A |
2: 119,880,939 (GRCm39) |
|
noncoding transcript |
Het |
| Srrm2 |
A |
G |
17: 24,037,249 (GRCm39) |
|
probably benign |
Het |
| Stx16 |
G |
A |
2: 173,935,292 (GRCm39) |
G156R |
probably damaging |
Het |
| Topbp1 |
A |
G |
9: 103,205,698 (GRCm39) |
K779E |
probably benign |
Het |
| Trank1 |
T |
A |
9: 111,220,294 (GRCm39) |
F2344I |
probably damaging |
Het |
| Ttll5 |
GCCCTGCGGGGCTGCCACGCTGTCGATCCGGCAGCTAC |
G |
12: 85,980,329 (GRCm39) |
|
probably null |
Het |
| Ube4a |
G |
A |
9: 44,864,395 (GRCm39) |
P66L |
probably damaging |
Het |
| Utp6 |
A |
T |
11: 79,844,424 (GRCm39) |
F200L |
probably benign |
Het |
| Vmn2r117 |
A |
T |
17: 23,696,176 (GRCm39) |
H410Q |
probably damaging |
Het |
| Vps33b |
C |
T |
7: 79,935,088 (GRCm39) |
H344Y |
probably benign |
Het |
| Xpo5 |
C |
T |
17: 46,552,772 (GRCm39) |
R1145* |
probably null |
Het |
| Zbed4 |
T |
A |
15: 88,665,852 (GRCm39) |
F640Y |
possibly damaging |
Het |
| Zfp618 |
C |
T |
4: 63,050,799 (GRCm39) |
R527C |
probably damaging |
Het |
|
| Other mutations in Or8k33 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01937:Or8k33
|
APN |
2 |
86,383,793 (GRCm39) |
missense |
probably benign |
0.34 |
|
IGL02169:Or8k33
|
APN |
2 |
86,384,226 (GRCm39) |
missense |
probably benign |
0.39 |
|
IGL02234:Or8k33
|
APN |
2 |
86,383,610 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02530:Or8k33
|
APN |
2 |
86,384,224 (GRCm39) |
missense |
possibly damaging |
0.60 |
|
R0046:Or8k33
|
UTSW |
2 |
86,383,976 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0360:Or8k33
|
UTSW |
2 |
86,384,123 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0364:Or8k33
|
UTSW |
2 |
86,384,123 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0377:Or8k33
|
UTSW |
2 |
86,383,927 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1496:Or8k33
|
UTSW |
2 |
86,384,096 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1609:Or8k33
|
UTSW |
2 |
86,383,949 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1674:Or8k33
|
UTSW |
2 |
86,384,204 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1816:Or8k33
|
UTSW |
2 |
86,384,011 (GRCm39) |
nonsense |
probably null |
|
|
R2870:Or8k33
|
UTSW |
2 |
86,383,928 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R2870:Or8k33
|
UTSW |
2 |
86,383,928 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R4387:Or8k33
|
UTSW |
2 |
86,384,298 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4915:Or8k33
|
UTSW |
2 |
86,384,399 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4924:Or8k33
|
UTSW |
2 |
86,383,853 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4987:Or8k33
|
UTSW |
2 |
86,383,579 (GRCm39) |
splice site |
probably null |
0.10 |
|
R6305:Or8k33
|
UTSW |
2 |
86,383,839 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R6829:Or8k33
|
UTSW |
2 |
86,383,613 (GRCm39) |
nonsense |
probably null |
|
|
R7152:Or8k33
|
UTSW |
2 |
86,383,673 (GRCm39) |
missense |
probably benign |
0.14 |
|
R7561:Or8k33
|
UTSW |
2 |
86,383,661 (GRCm39) |
missense |
probably benign |
0.45 |
|
R7963:Or8k33
|
UTSW |
2 |
86,383,639 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R8031:Or8k33
|
UTSW |
2 |
86,384,447 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8177:Or8k33
|
UTSW |
2 |
86,383,623 (GRCm39) |
missense |
noncoding transcript |
|
|
R8349:Or8k33
|
UTSW |
2 |
86,383,980 (GRCm39) |
missense |
probably benign |
0.22 |
|
R8449:Or8k33
|
UTSW |
2 |
86,383,980 (GRCm39) |
missense |
probably benign |
0.22 |
|
R8490:Or8k33
|
UTSW |
2 |
86,384,027 (GRCm39) |
missense |
probably benign |
0.06 |
|
R8787:Or8k33
|
UTSW |
2 |
86,384,297 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8801:Or8k33
|
UTSW |
2 |
86,383,727 (GRCm39) |
missense |
probably benign |
0.22 |
|
R8808:Or8k33
|
UTSW |
2 |
86,384,297 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9035:Or8k33
|
UTSW |
2 |
86,384,021 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9301:Or8k33
|
UTSW |
2 |
86,383,818 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
Z1088:Or8k33
|
UTSW |
2 |
86,384,310 (GRCm39) |
missense |
probably benign |
0.40 |
|
Z1191:Or8k33
|
UTSW |
2 |
86,384,471 (GRCm39) |
start gained |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- AGCTCACTGCCAATGAACAG -3'
(R):5'- AACATTGGGATTGTTTTGTCCTTCA -3'
Sequencing Primer
(F):5'- CTGTGTAGCACAAAGAGAGTAGG -3'
(R):5'- TCTGCTGATGACAGGTCT -3'
|
| Posted On |
2016-11-21 |
Incidental Mutation