Mutagenetix > Incidental Mutation

Incidental Mutation 'R5745:4933407L21Rik'

445741

Institutional Source

Beutler Lab

Gene Symbol

4933407L21Rik

Ensembl Gene

ENSMUSG00000026224

Gene Name

RIKEN cDNA 4933407L21 gene

Synonyms

MMRRC Submission

043198-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R5745 (G1)

Quality Score

142

Status

Not validated

Chromosome

Chromosomal Location

85856204-85859477 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to G at 85858995 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000027426 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027426] [ENSMUST00000129392]

AlphaFold

Q9D476

Predicted Effect

probably null
Transcript: ENSMUST00000027426

Predicted Effect

probably benign
Transcript: ENSMUST00000129392

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000188949

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000190367

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110082I17Rik	G	A	5: 139,349,828 (GRCm39)	R74W	probably damaging	Het
Adcy8	A	T	15: 64,792,320 (GRCm39)	I212N	possibly damaging	Het
Cobll1	T	C	2: 64,928,801 (GRCm39)	T879A	probably damaging	Het
Copb2	T	C	9: 98,456,164 (GRCm39)	S233P	probably damaging	Het
Cpa5	T	A	6: 30,630,436 (GRCm39)	M330K	probably damaging	Het
Dgcr8	A	T	16: 18,098,307 (GRCm39)	N361K	probably benign	Het
Dmxl1	A	G	18: 49,979,653 (GRCm39)	E96G	probably benign	Het
Dock8	T	A	19: 25,107,761 (GRCm39)	N830K	probably benign	Het
Ephb1	C	T	9: 102,072,633 (GRCm39)	D49N	probably benign	Het
Fer1l6	A	G	15: 58,443,238 (GRCm39)	I514V	probably benign	Het
Fpr1	A	G	17: 18,097,344 (GRCm39)	I215T	probably benign	Het
Hectd4	G	A	5: 121,491,565 (GRCm39)	V3668M	possibly damaging	Het
Ighv3-4	T	A	12: 114,217,388 (GRCm39)	I68L	probably benign	Het
Intu	A	G	3: 40,647,402 (GRCm39)		probably null	Het
Kel	C	T	6: 41,675,961 (GRCm39)	G243E	probably damaging	Het
Mycbp2	A	C	14: 103,393,889 (GRCm39)	S2781A	possibly damaging	Het
Myom2	T	A	8: 15,172,705 (GRCm39)	S1211T	probably benign	Het
Nrp1	A	T	8: 129,194,929 (GRCm39)	I462F	probably benign	Het
Or10d3	T	C	9: 39,461,987 (GRCm39)	Y60C	probably damaging	Het
Or6c5c	T	A	10: 129,299,307 (GRCm39)	I254N	probably damaging	Het
Pcsk1	A	C	13: 75,280,079 (GRCm39)	S635R	probably benign	Het
Pms1	A	T	1: 53,246,861 (GRCm39)	Y280*	probably null	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,127 (GRCm39)		probably benign	Het
Sema3b	C	A	9: 107,478,628 (GRCm39)	A356S	probably damaging	Het
Shoc2	C	A	19: 54,018,323 (GRCm39)	T485K	probably benign	Het
Slc7a7	G	A	14: 54,615,292 (GRCm39)	S235L	possibly damaging	Het
Smcr8	A	T	11: 60,674,977 (GRCm39)	T918S	probably benign	Het
Tafa1	G	A	6: 96,626,146 (GRCm39)	R128Q	probably damaging	Het
Tle3	C	A	9: 61,322,133 (GRCm39)	F719L	probably damaging	Het
Vmn2r45	T	A	7: 8,486,074 (GRCm39)	I405L	probably benign	Het
Vmn2r57	A	T	7: 41,097,895 (GRCm39)	H57Q	possibly damaging	Het

Other mutations in 4933407L21Rik

Allele	Source	Chr	Coord	Type	Predicted Effect
R0308:4933407L21Rik	UTSW	1	85,859,007 (GRCm39)	intron	probably benign
R0458:4933407L21Rik	UTSW	1	85,856,747 (GRCm39)	missense	unknown
R3155:4933407L21Rik	UTSW	1	85,859,104 (GRCm39)	intron	probably benign
R3156:4933407L21Rik	UTSW	1	85,859,104 (GRCm39)	intron	probably benign
R3886:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R3887:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R3888:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R3889:4933407L21Rik	UTSW	1	85,868,273 (GRCm39)	splice site	probably null
R4743:4933407L21Rik	UTSW	1	85,858,972 (GRCm39)	intron	probably benign
R4955:4933407L21Rik	UTSW	1	85,859,008 (GRCm39)	intron	probably benign
R7429:4933407L21Rik	UTSW	1	85,859,028 (GRCm39)	missense	unknown
R8257:4933407L21Rik	UTSW	1	85,859,060 (GRCm39)	nonsense	probably null
R8272:4933407L21Rik	UTSW	1	85,859,118 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GTAGCAATGGCACTGTCAGC -3'
(R):5'- TCATAATGCATGCCTCTCAAGG -3'

Sequencing Primer
(F):5'- TGTCAGCCACAGAGCCCTC -3'
(R):5'- CTCTCAAGGCAGCCAGC -3'

Posted On

2016-11-21