Mutagenetix > Incidental Mutation

Incidental Mutation 'R0029:Or8g35'

44577

Institutional Source

Beutler Lab

Gene Symbol

Or8g35

Ensembl Gene

ENSMUSG00000063176

Gene Name

olfactory receptor family 8 subfamily G member 35

Synonyms

Olfr955, MOR171-50, GA_x6K02T2PVTD-33167297-33166353, MOR171-34

MMRRC Submission

038323-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R0029 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

39381076-39382020 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 39381956 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Valine at position 22 (E22V)

Ref Sequence

ENSEMBL: ENSMUSP00000151246 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073946] [ENSMUST00000220176]

AlphaFold

Q9EQ97

Predicted Effect

probably benign
Transcript: ENSMUST00000073946
AA Change: E22V

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000073602
Gene: ENSMUSG00000063176
AA Change: E22V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.2e-46	PFAM
Pfam:7tm_1	41	290	1.2e-19	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216492

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219910

Predicted Effect

probably benign
Transcript: ENSMUST00000220176
AA Change: E22V

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.5%
20x: 93.6%

Validation Efficiency

94% (48/51)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	T	C	7: 119,945,225 (GRCm39)	F434L	probably benign	Het
Abt1	A	T	13: 23,606,678 (GRCm39)	F141Y	possibly damaging	Het
Anapc15-ps	A	G	10: 95,508,857 (GRCm39)	I141T	probably damaging	Het
Avl9	G	T	6: 56,713,468 (GRCm39)	R242L	probably benign	Het
Axin2	A	G	11: 108,814,873 (GRCm39)	T254A	probably benign	Het
Ccn4	C	T	15: 66,784,713 (GRCm39)	R129C	probably damaging	Het
Ciz1	A	G	2: 32,261,431 (GRCm39)		probably benign	Het
Cpa4	A	G	6: 30,585,044 (GRCm39)	Y276C	probably damaging	Het
Cpt1a	A	G	19: 3,431,674 (GRCm39)	D698G	probably benign	Het
Crebbp	T	C	16: 3,935,307 (GRCm39)	T861A	probably damaging	Het
Dpy19l2	T	A	9: 24,469,397 (GRCm39)	D753V	probably damaging	Het
Exosc7	A	T	9: 122,948,302 (GRCm39)		probably benign	Het
Fbxw28	T	A	9: 109,157,357 (GRCm39)	D244V	probably damaging	Het
Fgd5	A	G	6: 92,044,539 (GRCm39)	D1260G	probably benign	Het
Gapvd1	T	A	2: 34,568,153 (GRCm39)	I1404F	probably damaging	Het
Gas7	A	G	11: 67,534,163 (GRCm39)	S88G	probably benign	Het
Hk1	T	C	10: 62,151,173 (GRCm39)	D57G	probably damaging	Het
Il23r	A	C	6: 67,455,929 (GRCm39)		probably null	Het
Impg1	T	C	9: 80,305,653 (GRCm39)	D138G	probably damaging	Het
Itga2	G	A	13: 115,007,032 (GRCm39)	S432L	possibly damaging	Het
Kirrel2	A	G	7: 30,152,590 (GRCm39)		probably benign	Het
Lipm	T	C	19: 34,093,948 (GRCm39)		probably benign	Het
Lrpap1	T	C	5: 35,255,021 (GRCm39)	N205S	possibly damaging	Het
Mboat4	T	G	8: 34,587,363 (GRCm39)	F87V	probably damaging	Het
Nadsyn1	G	C	7: 143,359,815 (GRCm39)	Q386E	probably benign	Het
Nell1	G	A	7: 49,770,463 (GRCm39)		probably benign	Het
Or5ac25	T	C	16: 59,181,904 (GRCm39)	R226G	probably benign	Het
Pard3	G	T	8: 128,153,239 (GRCm39)		probably benign	Het
Per2	C	A	1: 91,351,434 (GRCm39)	R1024L	possibly damaging	Het
Phf11c	T	C	14: 59,622,364 (GRCm39)	D216G	probably benign	Het
Polk	G	A	13: 96,653,178 (GRCm39)	T74I	probably damaging	Het
Prmt6	T	C	3: 110,157,214 (GRCm39)	I358M	probably benign	Het
Psmb7	T	A	2: 38,523,919 (GRCm39)	H152L	probably damaging	Het
Ralgps1	A	T	2: 33,031,031 (GRCm39)	D498E	probably benign	Het
Slc26a2	G	A	18: 61,335,382 (GRCm39)	P24S	possibly damaging	Het
Slc4a11	A	G	2: 130,529,974 (GRCm39)	F268S	probably damaging	Het
Spmip11	T	C	15: 98,483,190 (GRCm39)		probably null	Het
Stk38	T	C	17: 29,201,112 (GRCm39)	E188G	probably benign	Het
Sulf2	T	C	2: 165,958,893 (GRCm39)	N105S	possibly damaging	Het
Sult2a3	T	A	7: 13,806,999 (GRCm39)	M228L	probably benign	Het
Svil	C	A	18: 5,063,286 (GRCm39)	D852E	probably benign	Het
Tcaf2	A	T	6: 42,607,093 (GRCm39)	L287*	probably null	Het
Tmem132e	A	T	11: 82,335,587 (GRCm39)	I890F	probably damaging	Het
Tmem63a	A	G	1: 180,790,031 (GRCm39)	Y401C	probably benign	Het
Ttn	T	C	2: 76,596,850 (GRCm39)	E20021G	probably damaging	Het
Ubac1	G	T	2: 25,911,455 (GRCm39)	T31N	probably benign	Het
Usp29	T	C	7: 6,964,580 (GRCm39)	L141P	probably damaging	Het
Vmn1r179	A	T	7: 23,628,630 (GRCm39)	I274F	probably benign	Het
Vmn1r204	A	G	13: 22,740,588 (GRCm39)	Y73C	probably benign	Het
Vmn2r2	T	C	3: 64,024,365 (GRCm39)	I739V	probably benign	Het

Other mutations in Or8g35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Or8g35	APN	9	39,381,539 (GRCm39)	missense	probably benign	0.00
IGL02550:Or8g35	APN	9	39,381,842 (GRCm39)	missense	probably benign	0.42
IGL02743:Or8g35	APN	9	39,381,542 (GRCm39)	missense	probably benign	0.10
R0329:Or8g35	UTSW	9	39,381,852 (GRCm39)	missense	possibly damaging	0.52
R0610:Or8g35	UTSW	9	39,381,119 (GRCm39)	missense	probably damaging	0.98
R1420:Or8g35	UTSW	9	39,381,289 (GRCm39)	missense	probably damaging	1.00
R1636:Or8g35	UTSW	9	39,381,215 (GRCm39)	missense	probably benign	0.03
R1937:Or8g35	UTSW	9	39,381,333 (GRCm39)	missense	possibly damaging	0.63
R2655:Or8g35	UTSW	9	39,381,924 (GRCm39)	missense	probably benign
R2944:Or8g35	UTSW	9	39,381,234 (GRCm39)	missense	possibly damaging	0.52
R3788:Or8g35	UTSW	9	39,381,365 (GRCm39)	missense	probably benign	0.03
R4829:Or8g35	UTSW	9	39,381,663 (GRCm39)	missense	probably damaging	0.99
R5625:Or8g35	UTSW	9	39,381,099 (GRCm39)	missense	probably benign
R6168:Or8g35	UTSW	9	39,381,953 (GRCm39)	missense	probably damaging	1.00
R6383:Or8g35	UTSW	9	39,381,926 (GRCm39)	missense	probably damaging	1.00
R6418:Or8g35	UTSW	9	39,381,112 (GRCm39)	missense	probably benign	0.07
R6645:Or8g35	UTSW	9	39,381,562 (GRCm39)	missense	probably benign	0.03
R7062:Or8g35	UTSW	9	39,381,353 (GRCm39)	missense	probably benign
R7765:Or8g35	UTSW	9	39,381,612 (GRCm39)	missense	probably benign	0.28
R7847:Or8g35	UTSW	9	39,381,801 (GRCm39)	missense	probably benign	0.42
R8122:Or8g35	UTSW	9	39,381,822 (GRCm39)	missense	probably damaging	1.00
R9578:Or8g35	UTSW	9	39,381,201 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CAGCCAACATGTGACATTCTGCAAC -3'
(R):5'- CCAGGACTGCATAATCTCTGAATGCTC -3'

Sequencing Primer
(F):5'- AGAACTGAGCTATGCATTCTGG -3'
(R):5'- CTGCATAATCTCTGAATGCTCAAATC -3'

Posted On

2013-06-11