Mutagenetix > Incidental Mutation

Incidental Mutation 'R5749:Zfp697'

445909

Institutional Source

Beutler Lab

Gene Symbol

Zfp697

Ensembl Gene

ENSMUSG00000050064

Gene Name

zinc finger protein 697

Synonyms

MMRRC Submission

043200-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.164) question?

Stock #

R5749 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

98289278-98508893 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 98332780 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 69 (S69P)

Ref Sequence

ENSEMBL: ENSMUSP00000136417 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056096] [ENSMUST00000178372]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000056096
AA Change: S69P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000056979
Gene: ENSMUSG00000050064
AA Change: S69P

Domain	Start	End	E-Value	Type
low complexity region	83	96	N/A	INTRINSIC
low complexity region	124	135	N/A	INTRINSIC
low complexity region	157	168	N/A	INTRINSIC
ZnF_C2H2	202	224	3.72e0	SMART
low complexity region	245	268	N/A	INTRINSIC
ZnF_C2H2	280	302	3.63e-3	SMART
ZnF_C2H2	308	330	1.76e-1	SMART
ZnF_C2H2	336	358	6.78e-3	SMART
ZnF_C2H2	377	399	2.57e-3	SMART
ZnF_C2H2	405	427	2.09e-3	SMART
ZnF_C2H2	433	455	7.26e-3	SMART
ZnF_C2H2	461	483	6.42e-4	SMART
ZnF_C2H2	489	511	2.91e-2	SMART
ZnF_C2H2	517	539	9.73e-4	SMART
ZnF_C2H2	545	567	1.43e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000178372
AA Change: S69P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000136417
Gene: ENSMUSG00000050064
AA Change: S69P

Domain	Start	End	E-Value	Type
low complexity region	83	96	N/A	INTRINSIC
low complexity region	124	135	N/A	INTRINSIC
low complexity region	157	168	N/A	INTRINSIC
ZnF_C2H2	202	224	3.72e0	SMART
low complexity region	245	268	N/A	INTRINSIC
ZnF_C2H2	280	302	3.63e-3	SMART
ZnF_C2H2	308	330	1.76e-1	SMART
ZnF_C2H2	336	358	6.78e-3	SMART
ZnF_C2H2	377	399	2.57e-3	SMART
ZnF_C2H2	405	427	2.09e-3	SMART
ZnF_C2H2	433	455	7.26e-3	SMART
ZnF_C2H2	461	483	6.42e-4	SMART
ZnF_C2H2	489	511	2.91e-2	SMART
ZnF_C2H2	517	539	9.73e-4	SMART
ZnF_C2H2	545	567	1.43e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196741

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsl6	G	A	11: 54,214,881 (GRCm39)		probably null	Het
Ankrd12	T	C	17: 66,293,091 (GRCm39)	S781G	probably benign	Het
Bicc1	A	G	10: 70,782,799 (GRCm39)	S523P	probably benign	Het
Ccdc163	T	A	4: 116,571,309 (GRCm39)	C44*	probably null	Het
Ccdc83	T	C	7: 89,873,156 (GRCm39)	T400A	probably damaging	Het
Cobl	A	G	11: 12,216,965 (GRCm39)	S426P	possibly damaging	Het
Cyp2b19	T	C	7: 26,462,844 (GRCm39)	I242T	possibly damaging	Het
Efnb2	A	C	8: 8,689,347 (GRCm39)	C92G	probably damaging	Het
Fam90a1a	T	A	8: 22,453,057 (GRCm39)	S137R	possibly damaging	Het
Fbxo17	G	A	7: 28,436,897 (GRCm39)	R284H	probably damaging	Het
Fem1b	A	G	9: 62,704,288 (GRCm39)	L324P	probably damaging	Het
Fsd1	T	A	17: 56,302,849 (GRCm39)		probably null	Het
Gtpbp4	A	G	13: 9,045,983 (GRCm39)		probably null	Het
Ifi209	A	C	1: 173,464,893 (GRCm39)	I8L	probably damaging	Het
Itga8	T	C	2: 12,266,889 (GRCm39)	E182G	probably damaging	Het
Itsn1	T	A	16: 91,703,743 (GRCm39)	L87H	probably damaging	Het
Klk1b16	T	C	7: 43,790,210 (GRCm39)	I160T	probably benign	Het
Lbp	T	A	2: 158,161,673 (GRCm39)	V52D	probably damaging	Het
Med23	T	C	10: 24,764,347 (GRCm39)	V318A	possibly damaging	Het
Myo16	C	T	8: 10,463,245 (GRCm39)	S604L	probably benign	Het
Or10ag52	C	T	2: 87,043,287 (GRCm39)	T17I	probably benign	Het
Or10g1	T	A	14: 52,647,961 (GRCm39)	M123L	probably damaging	Het
Or5b12	A	G	19: 12,897,589 (GRCm39)	V28A	probably benign	Het
Or6c38	A	T	10: 128,928,966 (GRCm39)	N292K	probably damaging	Het
Pcdh8	T	C	14: 80,007,525 (GRCm39)	D346G	probably damaging	Het
Ppara	A	T	15: 85,673,229 (GRCm39)	D140V	probably benign	Het
Prlr	T	A	15: 10,328,804 (GRCm39)	D426E	probably benign	Het
Prss36	T	A	7: 127,532,814 (GRCm39)	I192F	probably damaging	Het
Psg25	T	C	7: 18,258,776 (GRCm39)	E300G	probably damaging	Het
Pxylp1	A	G	9: 96,738,424 (GRCm39)	F26L	possibly damaging	Het
Rapgef4	A	T	2: 72,073,101 (GRCm39)	T796S	probably damaging	Het
Stard9	A	G	2: 120,534,267 (GRCm39)	H3508R	probably damaging	Het
Tep1	T	A	14: 51,081,529 (GRCm39)	D1282V	possibly damaging	Het
Tgfbr3l	A	G	8: 4,299,310 (GRCm39)	E59G	probably damaging	Het
Tnik	T	C	3: 28,648,241 (GRCm39)	M431T	probably benign	Het
Tns3	A	T	11: 8,401,177 (GRCm39)	H1040Q	probably benign	Het
Usp10	G	A	8: 120,667,872 (GRCm39)	E58K	probably damaging	Het
Vmn2r23	A	G	6: 123,710,232 (GRCm39)	T512A	probably benign	Het
Vmn2r52	C	T	7: 9,892,959 (GRCm39)	D727N	probably damaging	Het
Vmn2r66	T	A	7: 84,655,979 (GRCm39)	K346*	probably null	Het
Vmn2r93	T	A	17: 18,518,546 (GRCm39)	F2I	probably benign	Het
Zftraf1	A	C	15: 76,542,844 (GRCm39)		probably null	Het

Other mutations in Zfp697

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01449:Zfp697	APN	3	98,334,846 (GRCm39)	missense	probably damaging	0.99
IGL02313:Zfp697	APN	3	98,332,766 (GRCm39)	missense	probably benign	0.01
IGL03055:Zfp697	UTSW	3	98,332,810 (GRCm39)	missense	possibly damaging	0.51
R0724:Zfp697	UTSW	3	98,335,482 (GRCm39)	missense	probably damaging	1.00
R2165:Zfp697	UTSW	3	98,335,330 (GRCm39)	missense	unknown
R2971:Zfp697	UTSW	3	98,335,617 (GRCm39)	missense	probably damaging	1.00
R5954:Zfp697	UTSW	3	98,335,909 (GRCm39)	missense	probably damaging	0.99
R6253:Zfp697	UTSW	3	98,334,855 (GRCm39)	missense	possibly damaging	0.95
R8849:Zfp697	UTSW	3	98,334,943 (GRCm39)	missense	probably benign	0.38
R9300:Zfp697	UTSW	3	98,334,979 (GRCm39)	missense	possibly damaging	0.46
R9620:Zfp697	UTSW	3	98,335,182 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGAGCAGGGACTCTTGCTTAC -3'
(R):5'- GGAATGAATACTCATGGCACCAAC -3'

Sequencing Primer
(F):5'- TGTGTGTGAATACCAGACTTCTGAAG -3'
(R):5'- ACTTTTGGAGAGCCCACCC -3'

Posted On

2016-11-21