Mutagenetix > Incidental Mutation

Incidental Mutation 'R5762:Unc13c'

446064

Institutional Source

Beutler Lab

Gene Symbol

Unc13c

Ensembl Gene

ENSMUSG00000062151

Gene Name

unc-13 homolog C

Synonyms

D9Ertd414e, 1500037O19Rik, Munc13-3, Unc13h3

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5762 (G1)

Quality Score

128

Status

Not validated

Chromosome

Chromosomal Location

73386704-73876248 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 73719649 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 1006 (D1006E)

Ref Sequence

ENSEMBL: ENSMUSP00000139027 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075245] [ENSMUST00000184666]

AlphaFold

Q8K0T7

Predicted Effect

probably benign
Transcript: ENSMUST00000075245
AA Change: D1006E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000074726
Gene: ENSMUSG00000062151
AA Change: D1006E

Domain	Start	End	E-Value	Type
low complexity region	109	127	N/A	INTRINSIC
low complexity region	153	164	N/A	INTRINSIC
low complexity region	273	287	N/A	INTRINSIC
low complexity region	464	491	N/A	INTRINSIC
low complexity region	678	694	N/A	INTRINSIC
C1	1094	1143	1.37e-17	SMART
C2	1217	1324	1.46e-22	SMART
DUF1041	1535	1642	3.18e-51	SMART
Blast:DUF1041	1714	1838	2e-49	BLAST
Pfam:Membr_traf_MHD	1883	2023	2.6e-50	PFAM
C2	2058	2164	4.15e-13	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000184666
AA Change: D1006E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000139027
Gene: ENSMUSG00000062151
AA Change: D1006E

Domain	Start	End	E-Value	Type
low complexity region	109	127	N/A	INTRINSIC
low complexity region	153	164	N/A	INTRINSIC
low complexity region	273	287	N/A	INTRINSIC
low complexity region	464	491	N/A	INTRINSIC
low complexity region	678	694	N/A	INTRINSIC
C1	1094	1143	1.37e-17	SMART
C2	1217	1324	1.46e-22	SMART
DUF1041	1535	1642	3.18e-51	SMART
Blast:DUF1041	1714	1838	2e-49	BLAST
Pfam:Membr_traf_MHD	1882	2024	8.3e-59	PFAM
C2	2058	2164	4.15e-13	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous mutant mice demonstrate an impaired ability to learn complex motor tasks, putatively due to an observed increase in paired-pulse facilitation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	A	T	5: 77,044,445 (GRCm39)	D148E	probably benign	Het
Abca13	A	G	11: 9,531,665 (GRCm39)	I4631V	probably damaging	Het
Adamts16	A	T	13: 70,886,617 (GRCm39)	W1058R	probably damaging	Het
Adgrf5	A	G	17: 43,741,586 (GRCm39)	I163V	probably null	Het
Ano1	T	A	7: 144,201,774 (GRCm39)	Y338F	probably damaging	Het
Atp4a	A	G	7: 30,418,521 (GRCm39)	D603G	probably damaging	Het
Bmp2k	GGCCCGC	GGC	5: 97,235,050 (GRCm39)		probably null	Het
Brinp2	T	C	1: 158,074,156 (GRCm39)	D655G	probably benign	Het
C3ar1	A	G	6: 122,827,321 (GRCm39)	S299P	probably benign	Het
Calhm1	T	A	19: 47,132,058 (GRCm39)		probably null	Het
Ccdc187	G	A	2: 26,166,104 (GRCm39)	P775L	possibly damaging	Het
Cd27	A	G	6: 125,213,561 (GRCm39)	F48S	probably damaging	Het
Cfap52	A	G	11: 67,844,947 (GRCm39)	Y41H	possibly damaging	Het
Cntn5	A	G	9: 9,748,394 (GRCm39)	S701P	possibly damaging	Het
Ctbp2	G	T	7: 132,597,088 (GRCm39)	A665D	probably damaging	Het
Ctsd	C	A	7: 141,937,266 (GRCm39)	G81C	probably damaging	Het
Cybb	C	G	X: 9,316,989 (GRCm39)	D246H	probably benign	Het
Dpysl4	C	T	7: 138,671,853 (GRCm39)	A67V	probably benign	Het
Dst	C	T	1: 34,218,438 (GRCm39)	T1626I	probably damaging	Het
Etfdh	A	C	3: 79,523,261 (GRCm39)	D217E	probably null	Het
Far1	T	A	7: 113,167,396 (GRCm39)	Y494N	probably damaging	Het
Fndc1	G	A	17: 7,990,366 (GRCm39)	T1110M	unknown	Het
Frmd4a	G	T	2: 4,488,876 (GRCm39)	D78Y	probably damaging	Het
Fsip2	G	A	2: 82,808,260 (GRCm39)	M1526I	probably benign	Het
Ggn	C	T	7: 28,871,777 (GRCm39)	P399S	probably damaging	Het
H2ac6	C	T	13: 23,867,888 (GRCm39)	G5S	probably damaging	Het
Hap1	A	G	11: 100,246,600 (GRCm39)	W102R	probably damaging	Het
Herc2	T	A	7: 55,846,938 (GRCm39)	S3629R	possibly damaging	Het
Hyal4	A	C	6: 24,765,861 (GRCm39)	Y405S	possibly damaging	Het
Ifi204	G	A	1: 173,580,325 (GRCm39)	T395I	probably damaging	Het
Igsf9	A	G	1: 172,326,005 (GRCm39)	E1147G	probably damaging	Het
Inpp5a	T	A	7: 139,118,097 (GRCm39)	I225N	possibly damaging	Het
Kcnv1	T	C	15: 44,972,518 (GRCm39)	K455R	probably damaging	Het
Kmt2c	T	C	5: 25,515,455 (GRCm39)	D2796G	probably benign	Het
Ngp	A	T	9: 110,251,401 (GRCm39)	D143V	probably benign	Het
Nlrp5	T	C	7: 23,118,264 (GRCm39)	C663R	possibly damaging	Het
Nup205	G	T	6: 35,204,615 (GRCm39)	R1469L	probably damaging	Het
Nup205	T	A	6: 35,207,483 (GRCm39)	F1512I	probably damaging	Het
Nwd1	T	C	8: 73,397,542 (GRCm39)	S594P	probably damaging	Het
Nxpe2	A	C	9: 48,230,875 (GRCm39)	V498G	probably benign	Het
P3h4	G	A	11: 100,302,677 (GRCm39)	R320C	probably damaging	Het
Plec	A	G	15: 76,063,455 (GRCm39)	L2273P	probably damaging	Het
Ppp1r14b	C	T	19: 6,953,951 (GRCm39)	L100F	probably damaging	Het
Prlhr	C	T	19: 60,455,506 (GRCm39)	W353*	probably null	Het
Ralgps2	C	T	1: 156,660,234 (GRCm39)		probably null	Het
Rrp12	C	T	19: 41,868,591 (GRCm39)	G584D	possibly damaging	Het
Scamp3	T	C	3: 89,088,504 (GRCm39)	F237L	probably damaging	Het
Scn10a	A	G	9: 119,464,507 (GRCm39)		probably null	Het
Sh3bp5	C	A	14: 31,099,452 (GRCm39)	R265L	probably benign	Het
Shroom1	A	T	11: 53,354,818 (GRCm39)	D246V	probably benign	Het
Snapc4	T	A	2: 26,268,618 (GRCm39)	E14D	probably damaging	Het
Spag5	A	T	11: 78,194,972 (GRCm39)	Q93L	probably benign	Het
Tcstv5	T	A	13: 120,411,501 (GRCm39)	Q35L	probably benign	Het
Tle1	C	T	4: 72,038,372 (GRCm39)		probably null	Het
Ttll10	G	A	4: 156,119,438 (GRCm39)	P683S	possibly damaging	Het
Unc80	A	T	1: 66,732,955 (GRCm39)	K3101N	possibly damaging	Het
Vdac1	A	G	11: 52,278,280 (GRCm39)	Y247C	possibly damaging	Het
Vmn2r1	G	A	3: 63,997,474 (GRCm39)	V377I	probably benign	Het
Vmn2r23	A	G	6: 123,710,352 (GRCm39)	T552A	probably damaging	Het
Xrcc3	C	T	12: 111,771,044 (GRCm39)	R295Q	probably damaging	Het
Zfp780b	T	C	7: 27,664,243 (GRCm39)	N104S	probably benign	Het
Zkscan17	A	G	11: 59,378,397 (GRCm39)	V262A	possibly damaging	Het

Other mutations in Unc13c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00420:Unc13c	APN	9	73,643,985 (GRCm39)	missense	probably damaging	0.99
IGL00693:Unc13c	APN	9	73,665,884 (GRCm39)	missense	probably benign	0.18
IGL01022:Unc13c	APN	9	73,424,610 (GRCm39)	missense	probably benign	0.06
IGL01088:Unc13c	APN	9	73,839,563 (GRCm39)	missense	possibly damaging	0.63
IGL01123:Unc13c	APN	9	73,840,479 (GRCm39)	missense	probably benign	0.05
IGL01131:Unc13c	APN	9	73,471,335 (GRCm39)	missense	probably benign
IGL01135:Unc13c	APN	9	73,392,175 (GRCm39)	missense	probably damaging	1.00
IGL01393:Unc13c	APN	9	73,447,552 (GRCm39)	missense	probably benign	0.06
IGL01752:Unc13c	APN	9	73,839,093 (GRCm39)	missense	probably benign	0.01
IGL01893:Unc13c	APN	9	73,600,648 (GRCm39)	missense	probably benign	0.15
IGL01897:Unc13c	APN	9	73,453,309 (GRCm39)	missense	probably damaging	0.99
IGL01936:Unc13c	APN	9	73,600,524 (GRCm39)	missense	probably benign	0.07
IGL02122:Unc13c	APN	9	73,641,679 (GRCm39)	splice site	probably benign
IGL02341:Unc13c	APN	9	73,840,492 (GRCm39)	missense	possibly damaging	0.76
IGL02434:Unc13c	APN	9	73,839,910 (GRCm39)	missense	probably benign	0.01
IGL02545:Unc13c	APN	9	73,388,357 (GRCm39)	missense	probably damaging	0.98
IGL02709:Unc13c	APN	9	73,466,238 (GRCm39)	missense	probably benign	0.00
IGL02815:Unc13c	APN	9	73,447,545 (GRCm39)	missense	possibly damaging	0.83
IGL02904:Unc13c	APN	9	73,388,349 (GRCm39)	nonsense	probably null
IGL03117:Unc13c	APN	9	73,441,307 (GRCm39)	missense	probably benign	0.03
IGL03260:Unc13c	APN	9	73,838,626 (GRCm39)	missense	probably benign	0.11
Feeling	UTSW	9	73,600,553 (GRCm39)	missense	possibly damaging	0.46
Inkling	UTSW	9	73,839,126 (GRCm39)	missense	probably damaging	1.00
notion	UTSW	9	73,643,844 (GRCm39)	missense	probably damaging	1.00
BB001:Unc13c	UTSW	9	73,641,690 (GRCm39)	missense	probably benign	0.05
BB011:Unc13c	UTSW	9	73,641,690 (GRCm39)	missense	probably benign	0.05
PIT4431001:Unc13c	UTSW	9	73,656,829 (GRCm39)	missense	probably damaging	0.99
PIT4651001:Unc13c	UTSW	9	73,391,021 (GRCm39)	missense	possibly damaging	0.48
R0017:Unc13c	UTSW	9	73,600,583 (GRCm39)	missense	probably benign	0.07
R0039:Unc13c	UTSW	9	73,576,847 (GRCm39)	splice site	probably benign
R0164:Unc13c	UTSW	9	73,602,174 (GRCm39)	missense	probably benign	0.01
R0164:Unc13c	UTSW	9	73,602,174 (GRCm39)	missense	probably benign	0.01
R0308:Unc13c	UTSW	9	73,388,400 (GRCm39)	missense	probably benign	0.04
R0344:Unc13c	UTSW	9	73,838,067 (GRCm39)	missense	probably benign	0.39
R0421:Unc13c	UTSW	9	73,840,492 (GRCm39)	missense	possibly damaging	0.76
R0606:Unc13c	UTSW	9	73,438,265 (GRCm39)	splice site	probably benign
R0655:Unc13c	UTSW	9	73,838,235 (GRCm39)	missense	probably damaging	0.96
R1013:Unc13c	UTSW	9	73,840,614 (GRCm39)	missense	probably benign	0.45
R1293:Unc13c	UTSW	9	73,481,356 (GRCm39)	missense	probably benign	0.06
R1493:Unc13c	UTSW	9	73,546,350 (GRCm39)	missense	probably benign	0.27
R1675:Unc13c	UTSW	9	73,546,332 (GRCm39)	critical splice donor site	probably null
R1789:Unc13c	UTSW	9	73,663,621 (GRCm39)	missense	possibly damaging	0.92
R2001:Unc13c	UTSW	9	73,390,897 (GRCm39)	splice site	probably null
R2055:Unc13c	UTSW	9	73,643,832 (GRCm39)	missense	probably damaging	1.00
R2060:Unc13c	UTSW	9	73,572,938 (GRCm39)	missense	probably damaging	0.99
R2420:Unc13c	UTSW	9	73,838,829 (GRCm39)	missense	probably damaging	0.97
R2421:Unc13c	UTSW	9	73,838,829 (GRCm39)	missense	probably damaging	0.97
R2422:Unc13c	UTSW	9	73,838,829 (GRCm39)	missense	probably damaging	0.97
R3415:Unc13c	UTSW	9	73,839,868 (GRCm39)	missense	probably benign	0.00
R3423:Unc13c	UTSW	9	73,837,935 (GRCm39)	missense	possibly damaging	0.46
R3820:Unc13c	UTSW	9	73,838,240 (GRCm39)	missense	probably benign	0.00
R3857:Unc13c	UTSW	9	73,606,390 (GRCm39)	nonsense	probably null
R3859:Unc13c	UTSW	9	73,606,390 (GRCm39)	nonsense	probably null
R3895:Unc13c	UTSW	9	73,840,805 (GRCm39)	missense	probably benign
R4038:Unc13c	UTSW	9	73,441,188 (GRCm39)	critical splice donor site	probably null
R4077:Unc13c	UTSW	9	73,643,821 (GRCm39)	nonsense	probably null
R4125:Unc13c	UTSW	9	73,481,289 (GRCm39)	critical splice donor site	probably null
R4128:Unc13c	UTSW	9	73,641,819 (GRCm39)	missense	probably damaging	1.00
R4235:Unc13c	UTSW	9	73,438,234 (GRCm39)	missense	possibly damaging	0.68
R4295:Unc13c	UTSW	9	73,641,786 (GRCm39)	missense	probably damaging	1.00
R4307:Unc13c	UTSW	9	73,600,649 (GRCm39)	missense	probably benign	0.06
R4658:Unc13c	UTSW	9	73,840,108 (GRCm39)	missense	probably damaging	1.00
R4694:Unc13c	UTSW	9	73,479,636 (GRCm39)	missense	probably benign	0.00
R4735:Unc13c	UTSW	9	73,600,620 (GRCm39)	missense	probably benign	0.00
R4744:Unc13c	UTSW	9	73,839,126 (GRCm39)	missense	probably damaging	1.00
R4795:Unc13c	UTSW	9	73,839,469 (GRCm39)	missense	probably damaging	0.97
R4827:Unc13c	UTSW	9	73,838,568 (GRCm39)	missense	probably damaging	1.00
R4838:Unc13c	UTSW	9	73,839,354 (GRCm39)	missense	possibly damaging	0.68
R4869:Unc13c	UTSW	9	73,587,716 (GRCm39)	missense	probably benign	0.02
R4873:Unc13c	UTSW	9	73,424,566 (GRCm39)	missense	probably damaging	0.98
R4875:Unc13c	UTSW	9	73,424,566 (GRCm39)	missense	probably damaging	0.98
R4876:Unc13c	UTSW	9	73,656,821 (GRCm39)	missense	probably damaging	1.00
R4905:Unc13c	UTSW	9	73,587,674 (GRCm39)	missense	probably benign
R4912:Unc13c	UTSW	9	73,481,304 (GRCm39)	missense	probably damaging	0.99
R5026:Unc13c	UTSW	9	73,838,185 (GRCm39)	missense	possibly damaging	0.74
R5127:Unc13c	UTSW	9	73,840,654 (GRCm39)	missense	probably benign	0.26
R5151:Unc13c	UTSW	9	73,838,757 (GRCm39)	missense	probably benign	0.02
R5171:Unc13c	UTSW	9	73,665,236 (GRCm39)	missense	probably benign
R5244:Unc13c	UTSW	9	73,433,233 (GRCm39)	critical splice donor site	probably null
R5342:Unc13c	UTSW	9	73,838,105 (GRCm39)	missense	probably benign	0.00
R5399:Unc13c	UTSW	9	73,656,970 (GRCm39)	missense	possibly damaging	0.95
R5409:Unc13c	UTSW	9	73,485,672 (GRCm39)	missense	possibly damaging	0.78
R5460:Unc13c	UTSW	9	73,453,271 (GRCm39)	missense	probably benign
R5680:Unc13c	UTSW	9	73,839,884 (GRCm39)	missense	probably damaging	1.00
R5681:Unc13c	UTSW	9	73,453,357 (GRCm39)	splice site	probably null
R5728:Unc13c	UTSW	9	73,466,238 (GRCm39)	missense	probably benign	0.01
R5764:Unc13c	UTSW	9	73,441,185 (GRCm39)	splice site	probably null
R5829:Unc13c	UTSW	9	73,600,650 (GRCm39)	missense	probably benign	0.15
R5894:Unc13c	UTSW	9	73,600,486 (GRCm39)	critical splice donor site	probably null
R5936:Unc13c	UTSW	9	73,485,774 (GRCm39)	missense	probably damaging	1.00
R6043:Unc13c	UTSW	9	73,643,933 (GRCm39)	missense	possibly damaging	0.88
R6046:Unc13c	UTSW	9	73,838,166 (GRCm39)	missense	probably benign
R6148:Unc13c	UTSW	9	73,600,648 (GRCm39)	missense	probably benign	0.15
R6207:Unc13c	UTSW	9	73,665,910 (GRCm39)	missense	possibly damaging	0.89
R6277:Unc13c	UTSW	9	73,606,451 (GRCm39)	missense	probably damaging	1.00
R6338:Unc13c	UTSW	9	73,641,729 (GRCm39)	missense	probably damaging	0.99
R6615:Unc13c	UTSW	9	73,837,890 (GRCm39)	missense	possibly damaging	0.63
R6978:Unc13c	UTSW	9	73,839,259 (GRCm39)	missense	probably benign	0.39
R7053:Unc13c	UTSW	9	73,839,579 (GRCm39)	missense	probably damaging	1.00
R7223:Unc13c	UTSW	9	73,536,473 (GRCm39)	missense	probably benign	0.44
R7259:Unc13c	UTSW	9	73,424,645 (GRCm39)	missense	probably benign	0.00
R7353:Unc13c	UTSW	9	73,481,355 (GRCm39)	missense	probably benign	0.00
R7357:Unc13c	UTSW	9	73,840,811 (GRCm39)	small insertion	probably benign
R7357:Unc13c	UTSW	9	73,840,810 (GRCm39)	small insertion	probably benign
R7607:Unc13c	UTSW	9	73,576,817 (GRCm39)	missense	probably damaging	0.98
R7626:Unc13c	UTSW	9	73,641,799 (GRCm39)	missense	probably damaging	1.00
R7639:Unc13c	UTSW	9	73,840,450 (GRCm39)	missense	probably damaging	0.99
R7657:Unc13c	UTSW	9	73,441,185 (GRCm39)	splice site	probably null
R7665:Unc13c	UTSW	9	73,587,756 (GRCm39)	missense	probably benign	0.28
R7704:Unc13c	UTSW	9	73,606,494 (GRCm39)	missense	probably benign	0.27
R7776:Unc13c	UTSW	9	73,602,232 (GRCm39)	missense	probably damaging	1.00
R7811:Unc13c	UTSW	9	73,600,553 (GRCm39)	missense	possibly damaging	0.46
R7833:Unc13c	UTSW	9	73,388,391 (GRCm39)	missense	possibly damaging	0.53
R7839:Unc13c	UTSW	9	73,840,596 (GRCm39)	missense	possibly damaging	0.63
R7869:Unc13c	UTSW	9	73,602,159 (GRCm39)	missense	probably damaging	1.00
R7924:Unc13c	UTSW	9	73,641,690 (GRCm39)	missense	probably benign	0.05
R8047:Unc13c	UTSW	9	73,719,636 (GRCm39)	nonsense	probably null
R8167:Unc13c	UTSW	9	73,643,985 (GRCm39)	missense	probably damaging	0.99
R8202:Unc13c	UTSW	9	73,643,844 (GRCm39)	missense	probably damaging	1.00
R8210:Unc13c	UTSW	9	73,392,220 (GRCm39)	missense	probably benign	0.13
R8352:Unc13c	UTSW	9	73,838,290 (GRCm39)	missense	probably damaging	0.99
R8368:Unc13c	UTSW	9	73,838,070 (GRCm39)	missense	probably benign	0.15
R8452:Unc13c	UTSW	9	73,838,290 (GRCm39)	missense	probably damaging	0.99
R8535:Unc13c	UTSW	9	73,447,653 (GRCm39)	missense	probably benign
R8677:Unc13c	UTSW	9	73,840,243 (GRCm39)	missense	probably benign	0.00
R8700:Unc13c	UTSW	9	73,479,679 (GRCm39)	missense	probably benign	0.44
R8848:Unc13c	UTSW	9	73,433,263 (GRCm39)	missense	probably benign
R8902:Unc13c	UTSW	9	73,656,830 (GRCm39)	missense	probably damaging	0.97
R8953:Unc13c	UTSW	9	73,840,044 (GRCm39)	missense	probably benign	0.00
R8961:Unc13c	UTSW	9	73,839,524 (GRCm39)	missense	probably benign	0.06
R9015:Unc13c	UTSW	9	73,453,322 (GRCm39)	missense	probably benign
R9114:Unc13c	UTSW	9	73,719,665 (GRCm39)	missense	probably benign	0.02
R9217:Unc13c	UTSW	9	73,485,715 (GRCm39)	missense	probably damaging	1.00
R9252:Unc13c	UTSW	9	73,424,553 (GRCm39)	missense	possibly damaging	0.80
R9273:Unc13c	UTSW	9	73,839,862 (GRCm39)	missense	possibly damaging	0.96
R9317:Unc13c	UTSW	9	73,447,662 (GRCm39)	missense	possibly damaging	0.88
R9412:Unc13c	UTSW	9	73,839,772 (GRCm39)	missense	probably benign
R9505:Unc13c	UTSW	9	73,838,824 (GRCm39)	missense	probably benign	0.22
R9516:Unc13c	UTSW	9	73,392,220 (GRCm39)	missense	probably damaging	0.99
R9528:Unc13c	UTSW	9	73,837,960 (GRCm39)	missense	possibly damaging	0.48
R9567:Unc13c	UTSW	9	73,536,485 (GRCm39)	missense	probably damaging	0.99
R9756:Unc13c	UTSW	9	73,839,526 (GRCm39)	missense	probably benign	0.23
R9783:Unc13c	UTSW	9	73,392,227 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- ATGAGCATGCAGTGTAGTGTTAAG -3'
(R):5'- CTTTGACTTGGAGCTGACCC -3'

Sequencing Primer
(F):5'- GCATGCAGTGTAGTGTTAAGAAAAC -3'
(R):5'- ACTTGGAGCTGACCCTGCTTAG -3'

Posted On

2016-11-21