Mutagenetix > Incidental Mutation

Incidental Mutation 'R5766:Gm10549'

446309

Institutional Source

Beutler Lab

Gene Symbol

Gm10549

Ensembl Gene

ENSMUSG00000073610

Gene Name

predicted gene 10549

Synonyms

MMRRC Submission

044424-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R5766 (G1)

Quality Score

179

Status

Not validated

Chromosome

Chromosomal Location

33597216-33607763 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

C to A at 33597358 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000127787 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051087] [ENSMUST00000097634] [ENSMUST00000168890] [ENSMUST00000171533]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000051087

SMART Domains

Protein: ENSMUSP00000058132
Gene: ENSMUSG00000042834

Domain	Start	End	E-Value	Type
Pfam:Alveol-reg_P311	1	68	2.6e-41	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000097634
AA Change: A48D

SMART Domains

Protein: ENSMUSP00000095236
Gene: ENSMUSG00000073610
AA Change: A48D

Domain	Start	End	E-Value	Type
low complexity region	56	79	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000168890

SMART Domains

Protein: ENSMUSP00000130297
Gene: ENSMUSG00000042834

Domain	Start	End	E-Value	Type
Pfam:Alveol-reg_P311	1	68	2.6e-41	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171533

SMART Domains

Protein: ENSMUSP00000127787
Gene: ENSMUSG00000042834

Domain	Start	End	E-Value	Type
Pfam:Alveol-reg_P311	1	68	2.6e-41	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl3	A	G	4: 144,182,439 (GRCm39)	V343A	probably damaging	Het
AI429214	TCCCTGATGAAC	TC	8: 37,461,383 (GRCm39)		probably null	Het
Arid2	A	G	15: 96,270,086 (GRCm39)	T1400A	probably benign	Het
Arrdc1	G	A	2: 24,816,417 (GRCm39)	T184I	probably damaging	Het
Aspm	T	C	1: 139,406,740 (GRCm39)	F1876L	probably damaging	Het
Calhm1	C	T	19: 47,132,142 (GRCm39)	V158I	probably benign	Het
Caly	T	C	7: 139,650,310 (GRCm39)	K211E	probably benign	Het
Cenpe	T	C	3: 134,954,174 (GRCm39)	L1677P	probably damaging	Het
Cttnbp2	A	G	6: 18,381,032 (GRCm39)	V1388A	possibly damaging	Het
Dgkd	C	T	1: 87,808,171 (GRCm39)	R80*	probably null	Het
Dnah3	A	T	7: 119,577,445 (GRCm39)	I2072N	probably damaging	Het
Dnah8	A	G	17: 30,909,235 (GRCm39)	I1020M	probably benign	Het
Drc3	T	C	11: 60,284,647 (GRCm39)	V484A	probably benign	Het
Efcab8	A	G	2: 153,622,912 (GRCm39)	D27G	possibly damaging	Het
Fat4	G	A	3: 38,943,617 (GRCm39)	G837R	probably damaging	Het
Fcgbpl1	G	A	7: 27,836,754 (GRCm39)	W224*	probably null	Het
Fgb	A	T	3: 82,953,483 (GRCm39)	C96S	probably damaging	Het
Flt4	G	C	11: 49,517,513 (GRCm39)	W278C	possibly damaging	Het
Fmnl2	T	C	2: 52,991,466 (GRCm39)	V327A	probably damaging	Het
Fras1	A	T	5: 96,879,548 (GRCm39)	Y2455F	possibly damaging	Het
Gm13872	T	A	2: 102,568,254 (GRCm39)	I88N	probably damaging	Het
Hoxa6	A	G	6: 52,185,394 (GRCm39)	S71P	probably benign	Het
Hunk	T	A	16: 90,250,627 (GRCm39)	C148S	probably damaging	Het
Ighv1-18	A	C	12: 114,646,527 (GRCm39)	S26A	probably damaging	Het
Impg2	T	G	16: 56,080,183 (GRCm39)	D553E	possibly damaging	Het
Ints1	A	G	5: 139,757,900 (GRCm39)	I266T	probably benign	Het
Kpna3	G	T	14: 61,640,463 (GRCm39)	T33K	probably benign	Het
Lamb1	A	G	12: 31,349,930 (GRCm39)	D622G	probably damaging	Het
Lamp3	T	C	16: 19,520,067 (GRCm39)	T39A	probably damaging	Het
Mnt	C	T	11: 74,733,904 (GRCm39)		probably benign	Het
Msh4	A	G	3: 153,573,477 (GRCm39)	S726P	probably damaging	Het
Or13a25	T	A	7: 140,247,266 (GRCm39)	V22E	probably benign	Het
Pah	A	C	10: 87,403,209 (GRCm39)	K195Q	probably damaging	Het
Pdc	T	A	1: 150,209,251 (GRCm39)	*245K	probably null	Het
Pdzd8	A	T	19: 59,288,972 (GRCm39)	H809Q	possibly damaging	Het
Plekhd1	A	T	12: 80,769,140 (GRCm39)	I467L	probably benign	Het
Ppp2ca	A	G	11: 52,004,014 (GRCm39)	D57G	probably damaging	Het
Ralgapa1	A	T	12: 55,867,551 (GRCm39)	M1K	probably null	Het
Rfx2	T	A	17: 57,110,587 (GRCm39)	D133V	probably benign	Het
Scfd2	A	T	5: 74,623,312 (GRCm39)	L407Q	probably damaging	Het
Scp2d1	T	A	2: 144,665,957 (GRCm39)	S99T	possibly damaging	Het
Sec63	A	T	10: 42,677,677 (GRCm39)	N261I	probably damaging	Het
Slc8a1	T	C	17: 81,956,390 (GRCm39)	Y216C	probably damaging	Het
Son	T	A	16: 91,461,875 (GRCm39)		probably benign	Het
Syce1	T	C	7: 140,357,894 (GRCm39)	E285G	probably damaging	Het
Taar7a	G	T	10: 23,869,260 (GRCm39)	S40R	probably benign	Het
Ube3a	A	T	7: 58,925,807 (GRCm39)	D216V	possibly damaging	Het
Vgll2	A	G	10: 51,903,659 (GRCm39)	D174G	probably damaging	Het
Vmn2r17	A	T	5: 109,575,139 (GRCm39)	T149S	possibly damaging	Het
Zbtb39	G	T	10: 127,578,557 (GRCm39)	C377F	probably damaging	Het
Zfp820	T	A	17: 22,038,983 (GRCm39)	N115I	probably damaging	Het
Zfp948	T	C	17: 21,805,078 (GRCm39)	S23P	probably benign	Het

Other mutations in Gm10549

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL02648:Gm10549	APN	18	33,597,303 (GRCm39)	unclassified	probably benign
R0374:Gm10549	UTSW	18	33,597,235 (GRCm39)	unclassified	probably benign
R0668:Gm10549	UTSW	18	33,603,903 (GRCm39)	missense	unknown
R1806:Gm10549	UTSW	18	33,603,841 (GRCm39)	missense	unknown
R4214:Gm10549	UTSW	18	33,597,530 (GRCm39)	splice site	probably null
R4826:Gm10549	UTSW	18	33,603,838 (GRCm39)	missense	unknown
R5747:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R5748:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R5796:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6101:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6129:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6130:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6218:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6219:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6220:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6283:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6298:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6299:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6309:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6321:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6322:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6327:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6337:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6405:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6420:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6492:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6494:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R6505:Gm10549	UTSW	18	33,597,358 (GRCm39)	unclassified	probably benign
R7173:Gm10549	UTSW	18	33,597,462 (GRCm39)	missense	unknown
R7724:Gm10549	UTSW	18	33,603,912 (GRCm39)	nonsense	probably null
R7829:Gm10549	UTSW	18	33,597,463 (GRCm39)	unclassified	probably benign
R9356:Gm10549	UTSW	18	33,597,375 (GRCm39)	missense	unknown
R9358:Gm10549	UTSW	18	33,597,375 (GRCm39)	missense	unknown
R9359:Gm10549	UTSW	18	33,597,375 (GRCm39)	missense	unknown
R9584:Gm10549	UTSW	18	33,597,375 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- ATAATAAACCTGCGGCCGG -3'
(R):5'- TACAGGGGCTGAGAACTTTG -3'

Sequencing Primer
(F):5'- CGGGGCATCTGAGGGAAC -3'
(R):5'- TAGCGTCTCCGGTTGCAC -3'

Posted On

2016-11-21