Incidental Mutation 'R5776:Zfp641'
ID446729
Institutional Source Beutler Lab
Gene Symbol Zfp641
Ensembl Gene ENSMUSG00000022987
Gene Namezinc finger protein 641
Synonyms9930016F01Rik
MMRRC Submission 043375-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.155) question?
Stock #R5776 (G1)
Quality Score225
Status Validated
Chromosome15
Chromosomal Location98285585-98296161 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 98289010 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glycine at position 244 (D244G)
Ref Sequence ENSEMBL: ENSMUSP00000133212 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000023722] [ENSMUST00000169721]
Predicted Effect possibly damaging
Transcript: ENSMUST00000023722
AA Change: D230G

PolyPhen 2 Score 0.904 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000023722
Gene: ENSMUSG00000022987
AA Change: D230G

DomainStartEndE-ValueType
KRAB 91 142 2.16e-10 SMART
ZnF_C2H2 236 258 1.58e-3 SMART
ZnF_C2H2 264 286 1.67e-2 SMART
ZnF_C2H2 292 314 1.33e-1 SMART
ZnF_C2H2 342 364 8.94e-3 SMART
ZnF_C2H2 370 392 3.83e-2 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000169721
AA Change: D244G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000133212
Gene: ENSMUSG00000022987
AA Change: D244G

DomainStartEndE-ValueType
low complexity region 83 89 N/A INTRINSIC
KRAB 95 156 2.56e-21 SMART
ZnF_C2H2 250 272 1.58e-3 SMART
ZnF_C2H2 278 300 1.67e-2 SMART
ZnF_C2H2 306 328 1.33e-1 SMART
ZnF_C2H2 356 378 8.94e-3 SMART
ZnF_C2H2 384 406 3.83e-2 SMART
Meta Mutation Damage Score 0.074 question?
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.8%
  • 10x: 97.7%
  • 20x: 96.3%
Validation Efficiency 98% (64/65)
Allele List at MGI
Other mutations in this stock
Total: 60 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aass A T 6: 23,107,650 M378K possibly damaging Het
Abca17 A G 17: 24,295,158 V857A probably benign Het
Abca9 C T 11: 110,107,460 probably null Het
Adam22 T C 5: 8,127,361 K583E probably benign Het
Akr1c13 T C 13: 4,194,187 F80L probably damaging Het
Apob A T 12: 8,006,149 I1511F possibly damaging Het
Cfh C A 1: 140,144,023 R291I possibly damaging Het
Col1a1 C T 11: 94,949,724 S1114F unknown Het
Ctsj T C 13: 61,003,142 D168G probably damaging Het
Erf T C 7: 25,246,109 D79G probably damaging Het
Extl1 T C 4: 134,357,772 N629S possibly damaging Het
Fbxo9 T C 9: 78,095,187 E204G probably damaging Het
Fdx1l C A 9: 21,073,482 V9L possibly damaging Het
Gfer G T 17: 24,696,053 S5R probably benign Het
Gm15931 A T 7: 4,281,565 noncoding transcript Het
Gm6309 T A 5: 146,168,881 I158F possibly damaging Het
Gm7247 C T 14: 51,364,348 S26F probably benign Het
Gm8251 T C 1: 44,056,505 E1811G possibly damaging Het
Hacl1 A T 14: 31,622,871 N234K possibly damaging Het
Hcn3 C T 3: 89,148,105 A612T probably benign Het
Hectd1 T A 12: 51,764,114 E1679D possibly damaging Het
Hipk4 T C 7: 27,528,980 L285P probably damaging Het
Igsf3 G A 3: 101,425,480 V25I probably benign Het
Jade1 A G 3: 41,613,792 H765R probably benign Het
Kcnv1 T C 15: 45,114,567 D25G unknown Het
Klhl3 A G 13: 58,005,184 S586P probably benign Het
L3mbtl2 A G 15: 81,684,871 D582G probably damaging Het
Matn2 G A 15: 34,431,619 C835Y probably damaging Het
Mgea5 T C 19: 45,771,924 E265G probably damaging Het
Naa15 A C 3: 51,460,026 D490A probably damaging Het
Nab2 T A 10: 127,664,329 Y298F probably damaging Het
Nipsnap1 T A 11: 4,888,919 M115K probably benign Het
Nlrp5-ps T C 7: 14,592,724 noncoding transcript Het
Nol4l T G 2: 153,417,821 Q211P probably damaging Het
Olfr427 A T 1: 174,099,773 H105L probably damaging Het
Olfr625-ps1 T A 7: 103,683,039 M97K probably damaging Het
Pkhd1 T C 1: 20,209,185 K2970E possibly damaging Het
Plin4 T C 17: 56,104,983 T683A probably damaging Het
Ppm1g T C 5: 31,205,110 D282G probably benign Het
Ppp6r3 A G 19: 3,526,901 S10P possibly damaging Het
Raph1 G A 1: 60,490,156 probably benign Het
Rc3h2 A G 2: 37,378,313 V935A possibly damaging Het
Reg4 T A 3: 98,233,028 D108E possibly damaging Het
Saraf T A 8: 34,165,450 Y228N probably damaging Het
Sctr T C 1: 120,056,407 S357P probably damaging Het
Sec22b A T 3: 97,914,568 N139Y probably damaging Het
Sgsm1 T A 5: 113,250,957 I1037F probably damaging Het
Skint5 A C 4: 113,763,503 S671R unknown Het
Slc38a3 C A 9: 107,658,749 E62* probably null Het
Slfn14 C T 11: 83,283,599 E189K probably damaging Het
Sox21 A G 14: 118,235,244 L131P probably damaging Het
Stip1 A G 19: 7,022,025 probably null Het
Stx16 G A 2: 174,093,499 G156R probably damaging Het
Susd1 T C 4: 59,315,363 probably benign Het
Tdrd7 T A 4: 46,005,689 D498E probably benign Het
Tln2 C T 9: 67,258,250 E1053K probably damaging Het
Trav6-4 A G 14: 53,454,754 D103G probably damaging Het
Txndc15 A G 13: 55,718,107 E128G probably benign Het
Vmn2r22 A T 6: 123,637,714 W306R probably damaging Het
Yjefn3 A T 8: 69,889,471 L33Q probably damaging Het
Other mutations in Zfp641
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01639:Zfp641 APN 15 98291185 missense possibly damaging 0.85
IGL03092:Zfp641 APN 15 98290516 missense probably damaging 1.00
IGL03191:Zfp641 APN 15 98288687 missense probably damaging 0.98
R0079:Zfp641 UTSW 15 98289089 missense probably benign 0.28
R0243:Zfp641 UTSW 15 98289127 missense possibly damaging 0.82
R0487:Zfp641 UTSW 15 98289179 missense probably benign
R2092:Zfp641 UTSW 15 98293712 missense probably benign
R3415:Zfp641 UTSW 15 98290540 missense probably benign 0.28
R4834:Zfp641 UTSW 15 98293704 missense probably damaging 1.00
R4851:Zfp641 UTSW 15 98288717 missense probably damaging 0.99
R6057:Zfp641 UTSW 15 98292935 missense probably benign 0.00
R6896:Zfp641 UTSW 15 98293803 start codon destroyed probably benign 0.06
R6958:Zfp641 UTSW 15 98292951 missense possibly damaging 0.46
R6969:Zfp641 UTSW 15 98290567 missense possibly damaging 0.46
Predicted Primers PCR Primer
(F):5'- AACTCTTGCCGCACTCAGAG -3'
(R):5'- AGGGTGATACTCCTGAGCTACAG -3'

Sequencing Primer
(F):5'- GCACCTGCTTGGCTTGTCATG -3'
(R):5'- CTGAGCTACAGGTGGAACC -3'
Posted On2016-12-15