Mutagenetix > Incidental Mutation

Incidental Mutation 'R5777:Dtwd1'

446746

Institutional Source

Beutler Lab

Gene Symbol

Dtwd1

Ensembl Gene

ENSMUSG00000023330

Gene Name

DTW domain containing 1

Synonyms

1810033A06Rik

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5777 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

125994061-126007199 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 126001733 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 151 (D151G)

Ref Sequence

ENSEMBL: ENSMUSP00000127662 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110437] [ENSMUST00000170908]

AlphaFold

Q9D8U7

Predicted Effect

probably damaging
Transcript: ENSMUST00000110437
AA Change: D151G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000106067
Gene: ENSMUSG00000023330
AA Change: D151G

Domain	Start	End	E-Value	Type
DTW	65	293	2.33e-58	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000170908
AA Change: D151G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000127662
Gene: ENSMUSG00000023330
AA Change: D151G

Domain	Start	End	E-Value	Type
DTW	65	293	2.33e-58	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agrp	T	C	8: 106,294,000 (GRCm39)	E41G	probably benign	Het
Amph	A	T	13: 19,230,186 (GRCm39)	N45Y	probably damaging	Het
Atp11a	T	A	8: 12,882,522 (GRCm39)	L470Q	probably damaging	Het
C130073F10Rik	T	A	4: 101,747,946 (GRCm39)	Y76F	possibly damaging	Het
Casp12	A	T	9: 5,354,548 (GRCm39)	I306F	probably benign	Het
Cobll1	A	T	2: 64,933,612 (GRCm39)	M460K	probably benign	Het
Col6a4	A	T	9: 105,890,895 (GRCm39)	L1800I	possibly damaging	Het
Ctdsp1	T	C	1: 74,433,227 (GRCm39)	V131A	probably damaging	Het
Ctnna3	A	G	10: 64,511,664 (GRCm39)	H618R	probably benign	Het
Dhx38	C	A	8: 110,283,534 (GRCm39)	V538L	possibly damaging	Het
Fbxw28	A	G	9: 109,167,604 (GRCm39)	L51P	probably damaging	Het
Gm4781	C	T	10: 100,232,831 (GRCm39)		noncoding transcript	Het
Gm5134	C	A	10: 75,840,594 (GRCm39)	F479L	probably benign	Het
H2bc27	A	G	11: 58,839,835 (GRCm39)	K24E	probably benign	Het
Hexa	T	G	9: 59,468,243 (GRCm39)	V290G	probably damaging	Het
Ifi206	C	T	1: 173,308,928 (GRCm39)	R356K	possibly damaging	Het
Kcnj12	G	A	11: 60,961,277 (GRCm39)	R525Q	possibly damaging	Het
Lrp2	A	T	2: 69,285,869 (GRCm39)	I3774N	probably damaging	Het
Msh4	C	A	3: 153,569,076 (GRCm39)	M832I	probably benign	Het
Myh10	A	G	11: 68,676,685 (GRCm39)	E852G	probably damaging	Het
Ndufaf1	A	T	2: 119,490,963 (GRCm39)	C32*	probably null	Het
Nos2	A	G	11: 78,830,978 (GRCm39)	E387G	probably null	Het
Or4f58	C	T	2: 111,851,876 (GRCm39)	G108R	probably damaging	Het
Or55b10	A	G	7: 102,143,178 (GRCm39)	V268A	probably benign	Het
Or5h27	T	A	16: 59,006,266 (GRCm39)	L193F	unknown	Het
Or7a36	A	C	10: 78,820,512 (GRCm39)	D296A	possibly damaging	Het
Or7g29	A	T	9: 19,287,014 (GRCm39)	H54Q	probably benign	Het
Or8j3c	A	G	2: 86,253,669 (GRCm39)	V117A	probably benign	Het
P3h3	T	C	6: 124,832,921 (GRCm39)	T211A	probably benign	Het
Pcdh7	A	G	5: 57,876,856 (GRCm39)	N137S	probably damaging	Het
Pgf	T	C	12: 85,216,148 (GRCm39)	T157A	possibly damaging	Het
Prex1	T	C	2: 166,428,579 (GRCm39)	D714G	probably damaging	Het
Scn7a	T	C	2: 66,522,913 (GRCm39)	I930M	probably damaging	Het
Siglecg	T	C	7: 43,058,837 (GRCm39)	S197P	possibly damaging	Het
Skint7	T	A	4: 111,845,289 (GRCm39)	I367N	probably benign	Het
Slfn5	A	T	11: 82,851,830 (GRCm39)	D652V	probably damaging	Het
Stx1b	G	A	7: 127,410,090 (GRCm39)	Q3*	probably null	Het
Tex35	T	C	1: 156,934,777 (GRCm39)	M46V	probably benign	Het
Tkt	A	C	14: 30,280,733 (GRCm39)	T55P	possibly damaging	Het
Trim30c	T	C	7: 104,032,538 (GRCm39)	R263G	probably benign	Het
U2af2	G	A	7: 5,069,450 (GRCm39)	R33Q	probably benign	Het
Washc4	T	A	10: 83,391,469 (GRCm39)	V182D	probably damaging	Het
Xirp2	T	C	2: 67,340,348 (GRCm39)	V863A	possibly damaging	Het
Zfp976	T	G	7: 42,263,504 (GRCm39)	H111P	probably benign	Het
Zfp982	A	T	4: 147,595,321 (GRCm39)	N48Y	probably damaging	Het

Other mutations in Dtwd1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01328:Dtwd1	APN	2	126,006,739 (GRCm39)	missense	probably damaging	1.00
IGL01380:Dtwd1	APN	2	126,001,847 (GRCm39)	missense	probably benign	0.25
IGL02097:Dtwd1	APN	2	126,006,715 (GRCm39)	missense	probably damaging	1.00
R0991:Dtwd1	UTSW	2	126,001,940 (GRCm39)	missense	probably damaging	1.00
R1103:Dtwd1	UTSW	2	125,996,643 (GRCm39)	missense	probably damaging	1.00
R2145:Dtwd1	UTSW	2	126,001,904 (GRCm39)	missense	probably damaging	1.00
R5249:Dtwd1	UTSW	2	125,996,694 (GRCm39)	missense	probably benign
R5874:Dtwd1	UTSW	2	126,000,359 (GRCm39)	missense	probably damaging	1.00
R5951:Dtwd1	UTSW	2	126,000,342 (GRCm39)	missense	probably benign	0.36
R7261:Dtwd1	UTSW	2	126,000,424 (GRCm39)	missense	probably benign	0.00
R7829:Dtwd1	UTSW	2	126,006,679 (GRCm39)	missense	probably damaging	1.00
R8356:Dtwd1	UTSW	2	126,000,451 (GRCm39)	missense	probably damaging	0.98
R8456:Dtwd1	UTSW	2	126,000,451 (GRCm39)	missense	probably damaging	0.98
R8770:Dtwd1	UTSW	2	125,996,727 (GRCm39)	missense	probably damaging	0.99
R9278:Dtwd1	UTSW	2	126,006,728 (GRCm39)	missense	probably damaging	1.00
R9746:Dtwd1	UTSW	2	125,996,595 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTTTTGAAGTAACTTCTCCAGGTAG -3'
(R):5'- TCCAGGTGCTGTCAATAAAGAC -3'

Sequencing Primer
(F):5'- TGATTACATGTTCTTAGGAATGAGC -3'
(R):5'- AGACAACTCTTTTCAACTCGGGG -3'

Posted On

2016-12-15