Mutagenetix > Incidental Mutation

Incidental Mutation 'R5793:Zfp772'

447071

Institutional Source

Beutler Lab

Gene Symbol

Zfp772

Ensembl Gene

ENSMUSG00000066838

Gene Name

zinc finger protein 772

Synonyms

MMRRC Submission

043208-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R5793 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

7205121-7212997 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 7207283 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 136 (T136I)

Ref Sequence

ENSEMBL: ENSMUSP00000074055 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074455]

AlphaFold

Q3UQL6

Predicted Effect

probably benign
Transcript: ENSMUST00000074455
AA Change: T136I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000074055
Gene: ENSMUSG00000066838
AA Change: T136I

Domain	Start	End	E-Value	Type
KRAB	42	102	1.87e-14	SMART
ZnF_C2H2	193	215	1.36e-2	SMART
ZnF_C2H2	221	243	5.21e-4	SMART
ZnF_C2H2	249	271	1.38e-3	SMART
ZnF_C2H2	277	299	4.24e-4	SMART
ZnF_C2H2	305	327	5.21e-4	SMART
ZnF_C2H2	333	355	2.27e-4	SMART
ZnF_C2H2	361	383	1.18e-2	SMART
ZnF_C2H2	389	411	4.87e-4	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alox12	C	A	11: 70,133,879 (GRCm39)	R522L	probably benign	Het
Arfgef1	A	T	1: 10,279,753 (GRCm39)	D271E	probably benign	Het
Arg1	C	T	10: 24,796,540 (GRCm39)	V96M	probably benign	Het
Axin1	T	C	17: 26,362,282 (GRCm39)	S209P	probably damaging	Het
B4galnt3	A	G	6: 120,185,865 (GRCm39)		probably null	Het
Cdh23	T	A	10: 60,141,907 (GRCm39)	D3058V	probably damaging	Het
Chd9	A	G	8: 91,728,384 (GRCm39)	T794A	probably damaging	Het
Ckap5	C	T	2: 91,450,180 (GRCm39)	T1891I	possibly damaging	Het
Cspg4b	G	T	13: 113,457,556 (GRCm39)	V1201L	possibly damaging	Het
Dzank1	T	C	2: 144,348,144 (GRCm39)	I207M	probably benign	Het
Fkbp9	G	A	6: 56,850,498 (GRCm39)		probably null	Het
Gm4781	T	A	10: 100,232,529 (GRCm39)		noncoding transcript	Het
Gprc5c	G	T	11: 114,755,093 (GRCm39)	V257L	possibly damaging	Het
Gtpbp6	G	A	5: 110,255,094 (GRCm39)	L33F	probably benign	Het
Hsd11b1	A	C	1: 192,924,492 (GRCm39)	F27V	probably damaging	Het
Ift172	A	T	5: 31,434,292 (GRCm39)	I482N	possibly damaging	Het
Kcnma1	C	T	14: 23,359,103 (GRCm39)		probably null	Het
Ly6g2	T	C	15: 75,093,493 (GRCm39)		probably benign	Het
Myh9	T	C	15: 77,653,077 (GRCm39)	Q1420R	probably benign	Het
Ncam2	G	A	16: 81,372,991 (GRCm39)	V569I	possibly damaging	Het
Nsd1	T	C	13: 55,395,819 (GRCm39)	V1140A	probably benign	Het
Ogdhl	T	A	14: 32,054,730 (GRCm39)	L226Q	probably damaging	Het
Or51a24	T	C	7: 103,734,237 (GRCm39)	T17A	probably benign	Het
Orc2	T	C	1: 58,536,547 (GRCm39)	M1V	probably null	Het
Padi2	T	C	4: 140,660,501 (GRCm39)	L327P	probably benign	Het
Palb2	T	C	7: 121,726,860 (GRCm39)	N337D	probably benign	Het
Pard3b	A	T	1: 61,807,132 (GRCm39)	H49L	probably damaging	Het
Pigz	T	C	16: 31,764,285 (GRCm39)	S448P	probably benign	Het
Ppfia4	A	G	1: 134,239,844 (GRCm39)	V911A	probably damaging	Het
Prr5	T	A	15: 84,656,223 (GRCm39)	M408K	probably benign	Het
Qser1	T	C	2: 104,593,205 (GRCm39)	Y1604C	probably damaging	Het
Rpgrip1l	A	G	8: 91,987,400 (GRCm39)	S886P	probably benign	Het
Sdk2	T	C	11: 113,759,778 (GRCm39)	I408V	possibly damaging	Het
Sema7a	A	T	9: 57,867,540 (GRCm39)	R431W	probably damaging	Het
Slc22a30	T	A	19: 8,314,183 (GRCm39)	Y501F	possibly damaging	Het
Slitrk5	T	A	14: 111,917,345 (GRCm39)	V323D	probably damaging	Het
Snap47	C	T	11: 59,329,018 (GRCm39)	E95K	probably damaging	Het
Tbc1d9	A	T	8: 83,998,069 (GRCm39)	I1209F	probably damaging	Het
Tek	T	A	4: 94,708,333 (GRCm39)	M297K	probably benign	Het
Tmem132e	T	A	11: 82,335,684 (GRCm39)	I922N	probably damaging	Het
Tmx2	T	C	2: 84,506,501 (GRCm39)	R64G	probably damaging	Het
Trim68	A	T	7: 102,333,560 (GRCm39)	S41T	possibly damaging	Het
Tsga10	A	T	1: 37,874,540 (GRCm39)	M115K	probably damaging	Het
Ttc23l	G	T	15: 10,551,636 (GRCm39)	T30K	possibly damaging	Het
Vmn2r84	C	T	10: 130,221,754 (GRCm39)	C822Y	probably damaging	Het
Wdr72	C	T	9: 74,117,625 (GRCm39)	A779V	probably benign	Het
Zfp580	A	T	7: 5,055,891 (GRCm39)		probably benign	Het

Other mutations in Zfp772

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00480:Zfp772	APN	7	7,207,115 (GRCm39)	missense	probably benign
IGL01589:Zfp772	APN	7	7,208,523 (GRCm39)	missense	possibly damaging	0.53
PIT4449001:Zfp772	UTSW	7	7,207,350 (GRCm39)	missense	probably benign	0.03
R1945:Zfp772	UTSW	7	7,206,629 (GRCm39)	missense	probably benign	0.01
R3085:Zfp772	UTSW	7	7,206,699 (GRCm39)	missense	possibly damaging	0.53
R5300:Zfp772	UTSW	7	7,207,157 (GRCm39)	missense	probably benign
R6252:Zfp772	UTSW	7	7,207,018 (GRCm39)	missense	possibly damaging	0.86
R6605:Zfp772	UTSW	7	7,208,547 (GRCm39)	missense	possibly damaging	0.72
R6751:Zfp772	UTSW	7	7,206,716 (GRCm39)	missense	possibly damaging	0.90
R6812:Zfp772	UTSW	7	7,209,307 (GRCm39)	missense	possibly damaging	0.92
R8171:Zfp772	UTSW	7	7,207,096 (GRCm39)	nonsense	probably null
R8696:Zfp772	UTSW	7	7,208,518 (GRCm39)	missense	possibly damaging	0.53
R9104:Zfp772	UTSW	7	7,207,190 (GRCm39)	missense	possibly damaging	0.53
R9665:Zfp772	UTSW	7	7,212,793 (GRCm39)	start codon destroyed	probably null

Predicted Primers

PCR Primer
(F):5'- GCCACAGTCACTGCATATGC -3'
(R):5'- TTCAGCATGGTGTTGGACC -3'

Sequencing Primer
(F):5'- GCAGTTCTTCTTCTCACTCTGAATGG -3'
(R):5'- TGTTCTGTGAGAAACCTCAGATCACC -3'

Posted On

2016-12-15