Mutagenetix > Incidental Mutation

Incidental Mutation 'R5796:Fgl1'

447233

Institutional Source

Beutler Lab

Gene Symbol

Fgl1

Ensembl Gene

ENSMUSG00000031594

Gene Name

fibrinogen-like protein 1

Synonyms

MMRRC Submission

043387-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R5796 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41644471-41668193 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to G at 41652796 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000034003 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034003]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000034003

SMART Domains

Protein: ENSMUSP00000034003
Gene: ENSMUSG00000031594

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
FBG	80	307	1.4e-131	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134510

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.6%
20x: 96.1%

Validation Efficiency

93% (51/55)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased body weight, decreased circulating cholesterol and free fatty acid, hyperglycemia, impaired glucose tolerance, increased gluconeogenesis, increased white adipose tissue and decreased respiratory quotient. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	T	C	3: 124,215,077 (GRCm39)	T9A	possibly damaging	Het
Abcd3	G	A	3: 121,578,147 (GRCm39)	R160W	probably damaging	Het
Ap3d1	G	T	10: 80,549,871 (GRCm39)	T758K	possibly damaging	Het
Ate1	T	C	7: 130,068,998 (GRCm39)	Y423C	probably damaging	Het
Bsn	C	T	9: 108,003,223 (GRCm39)	G394D	probably damaging	Het
Cacna1d	T	C	14: 29,788,073 (GRCm39)	D1599G	probably damaging	Het
Cd33	A	G	7: 43,182,480 (GRCm39)		probably null	Het
Chct1	A	G	11: 85,064,101 (GRCm39)	T81A	probably null	Het
Col12a1	T	C	9: 79,611,111 (GRCm39)	N154D	possibly damaging	Het
Crocc	TCTGAGCTGCTGAGCTGC	TCTGAGCTGC	4: 140,769,118 (GRCm39)		probably null	Het
Cts3	A	T	13: 61,716,517 (GRCm39)	Y40N	probably damaging	Het
Cyfip2	T	C	11: 46,089,823 (GRCm39)	N1197D	probably benign	Het
Dclre1b	G	A	3: 103,714,773 (GRCm39)	Q77*	probably null	Het
Dsc3	T	A	18: 20,104,558 (GRCm39)	M590L	probably benign	Het
Efcab14	A	T	4: 115,603,780 (GRCm39)	I153F	probably damaging	Het
Fbxo42	T	C	4: 140,927,100 (GRCm39)	V460A	probably benign	Het
Gbf1	T	C	19: 46,272,782 (GRCm39)	S1697P	probably benign	Het
Gm10549	C	A	18: 33,597,358 (GRCm39)		probably benign	Het
Gm15056	C	A	8: 21,391,998 (GRCm39)		probably benign	Het
Gm17409	T	A	2: 58,361,034 (GRCm39)		probably benign	Het
Gm20730	A	G	6: 43,058,464 (GRCm39)	L116P	probably damaging	Het
Grb2	T	G	11: 115,536,698 (GRCm39)	H184P	probably benign	Het
Hepacam2	G	C	6: 3,466,200 (GRCm39)		probably null	Het
Hsd3b9	A	T	3: 98,354,168 (GRCm39)	D110E	probably benign	Het
Igkv4-55	T	A	6: 69,584,432 (GRCm39)	K60M	possibly damaging	Het
Itfg1	T	A	8: 86,445,522 (GRCm39)	H603L	probably damaging	Het
Katna1	C	A	10: 7,636,575 (GRCm39)	D366E	probably damaging	Het
Klk1b26	A	G	7: 43,665,752 (GRCm39)	Y188C	probably damaging	Het
Ldhd	T	C	8: 112,353,722 (GRCm39)	T464A	probably benign	Het
Muc5b	T	C	7: 141,411,133 (GRCm39)	S1360P	unknown	Het
Nckipsd	T	G	9: 108,688,813 (GRCm39)	V116G	probably benign	Het
Nhsl1	C	T	10: 18,399,998 (GRCm39)	S374L	probably benign	Het
Oxt	G	A	2: 130,418,533 (GRCm39)	G48D	probably damaging	Het
Pramel21	A	G	4: 143,341,778 (GRCm39)	N69S	probably benign	Het
Ptprk	C	T	10: 28,259,571 (GRCm39)	T337I	probably damaging	Het
Rad54b	A	T	4: 11,615,446 (GRCm39)	D818V	probably benign	Het
Rbpjl	T	C	2: 164,252,168 (GRCm39)		probably benign	Het
Recql5	T	A	11: 115,818,691 (GRCm39)		probably benign	Het
Robo4	A	T	9: 37,322,970 (GRCm39)	D830V	probably benign	Het
Rps6kb1	C	T	11: 86,402,677 (GRCm39)	G339S	probably benign	Het
Rtel1	G	A	2: 180,982,299 (GRCm39)	E350K	probably benign	Het
Rtn3	A	G	19: 7,434,832 (GRCm39)	S368P	possibly damaging	Het
Smad5	A	G	13: 56,871,645 (GRCm39)	H80R	probably damaging	Het
Sppl2c	T	A	11: 104,078,619 (GRCm39)	M473K	probably benign	Het
Tbrg1	A	G	9: 37,563,871 (GRCm39)		probably benign	Het
Tcf7	A	G	11: 52,152,354 (GRCm39)	I65T	probably benign	Het
Tgm2	T	C	2: 157,960,824 (GRCm39)	D618G	probably benign	Het
Tuba3b	C	T	6: 145,565,408 (GRCm39)	T292I	probably damaging	Het
Vmn1r63	G	A	7: 5,806,140 (GRCm39)	S164L	probably benign	Het
Zfp382	A	G	7: 29,832,774 (GRCm39)	K142E	probably damaging	Het
Zmym3	G	A	X: 100,459,406 (GRCm39)	P308L	probably benign	Het

Other mutations in Fgl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01619:Fgl1	APN	8	41,650,008 (GRCm39)	missense	probably damaging	1.00
IGL03158:Fgl1	APN	8	41,662,810 (GRCm39)	missense	probably benign	0.00
IGL02796:Fgl1	UTSW	8	41,650,095 (GRCm39)	missense	probably benign	0.01
R0639:Fgl1	UTSW	8	41,644,661 (GRCm39)	missense	probably benign	0.27
R0673:Fgl1	UTSW	8	41,644,661 (GRCm39)	missense	probably benign	0.27
R1413:Fgl1	UTSW	8	41,644,638 (GRCm39)	missense	possibly damaging	0.82
R1458:Fgl1	UTSW	8	41,663,496 (GRCm39)	missense	possibly damaging	0.54
R1603:Fgl1	UTSW	8	41,650,055 (GRCm39)	missense	probably damaging	1.00
R1951:Fgl1	UTSW	8	41,650,387 (GRCm39)	missense	probably benign	0.02
R5686:Fgl1	UTSW	8	41,653,594 (GRCm39)	nonsense	probably null
R6052:Fgl1	UTSW	8	41,653,548 (GRCm39)	missense	probably damaging	1.00
R7391:Fgl1	UTSW	8	41,663,483 (GRCm39)	missense	probably benign	0.04
R7492:Fgl1	UTSW	8	41,644,624 (GRCm39)	nonsense	probably null
R8114:Fgl1	UTSW	8	41,644,620 (GRCm39)	missense	probably damaging	0.99
R8140:Fgl1	UTSW	8	41,653,646 (GRCm39)	splice site	probably null
R8809:Fgl1	UTSW	8	41,650,368 (GRCm39)	nonsense	probably null
R8824:Fgl1	UTSW	8	41,652,748 (GRCm39)	missense	probably benign	0.00
R8930:Fgl1	UTSW	8	41,662,868 (GRCm39)	missense	probably benign	0.44
R8932:Fgl1	UTSW	8	41,662,868 (GRCm39)	missense	probably benign	0.44
R8983:Fgl1	UTSW	8	41,653,496 (GRCm39)	missense	probably benign	0.38

Predicted Primers

PCR Primer
(F):5'- GGATTATGGCTCAGCAAGAATCA -3'
(R):5'- GAGTTAAGCCTTCTTATGTCTCAGA -3'

Sequencing Primer
(F):5'- GCCTATGATCTAGTGGGAAATACTGC -3'
(R):5'- TGTCTCAGATTTATTCATGTGACAG -3'

Posted On

2016-12-15