Mutagenetix > Incidental Mutation

Incidental Mutation 'R5800:Gpr153'

447341

Institutional Source

Beutler Lab

Gene Symbol

Gpr153

Ensembl Gene

ENSMUSG00000042804

Gene Name

G protein-coupled receptor 153

Synonyms

PGR1, 1110065N12Rik

MMRRC Submission

043389-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5800 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

152358689-152369794 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 152364534 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 197 (Q197*)

Ref Sequence

ENSEMBL: ENSMUSP00000101276 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055754] [ENSMUST00000105650] [ENSMUST00000105651]

AlphaFold

Q8K0Z9

Predicted Effect

probably null
Transcript: ENSMUST00000055754
AA Change: Q197*

SMART Domains

Protein: ENSMUSP00000052742
Gene: ENSMUSG00000042804
AA Change: Q197*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	298	1.2e-14	PFAM
low complexity region	501	518	N/A	INTRINSIC
low complexity region	605	617	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000105650
AA Change: Q197*

SMART Domains

Protein: ENSMUSP00000101275
Gene: ENSMUSG00000042804
AA Change: Q197*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	297	5.4e-18	PFAM
low complexity region	478	495	N/A	INTRINSIC
low complexity region	582	594	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000105651
AA Change: Q197*

SMART Domains

Protein: ENSMUSP00000101276
Gene: ENSMUSG00000042804
AA Change: Q197*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	297	5.3e-17	PFAM
low complexity region	501	518	N/A	INTRINSIC
low complexity region	605	617	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144035

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.4%

Validation Efficiency

96% (50/52)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an integral membrane protein that belongs to the Class A rhodopsin superfamily of G protein coupled receptors. The encoded protein is expressed primarily in the central nervous system. A knockdown of the orthologous gene in rat is associated with a significant reduction in food intake and impaired decision making ability. Mutations in this gene are associated with schizophrenia, autism, and other neuropsychiatric disorders. The expression of this gene is activated by the glioma-associated oncogene homolog 1 transcription factor which, in turn, is activated by sonic hedgehog in normal and tumorigenic cells. [provided by RefSeq, Feb 2017]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	A	T	1: 71,360,591 (GRCm39)	V540D	possibly damaging	Het
Adamts8	A	T	9: 30,865,778 (GRCm39)	D442V	probably damaging	Het
B3galt9	T	C	2: 34,728,654 (GRCm39)	F151S	possibly damaging	Het
Bltp1	A	G	3: 37,106,592 (GRCm39)	D4974G	probably damaging	Het
Casp4	G	A	9: 5,308,915 (GRCm39)		probably null	Het
Cfap45	T	A	1: 172,366,167 (GRCm39)	V30E	probably damaging	Het
Col6a4	A	G	9: 105,957,474 (GRCm39)	F117L	probably damaging	Het
Dnah7c	A	T	1: 46,686,175 (GRCm39)	T1810S	probably benign	Het
Drosha	C	T	15: 12,865,153 (GRCm39)	T627M	probably damaging	Het
Drosha	C	A	15: 12,902,733 (GRCm39)	A1001D	probably damaging	Het
Efhc1	G	A	1: 21,049,005 (GRCm39)	V473I	probably benign	Het
Ephx2	T	C	14: 66,344,751 (GRCm39)	K191R	probably benign	Het
Ero1b	T	A	13: 12,617,190 (GRCm39)		probably null	Het
Esyt2	A	T	12: 116,333,808 (GRCm39)	D837V	possibly damaging	Het
Fip1l1	T	A	5: 74,706,742 (GRCm39)	D126E	possibly damaging	Het
Fyttd1	C	T	16: 32,711,658 (GRCm39)	R86C	probably damaging	Het
Gm12888	T	A	4: 121,176,625 (GRCm39)	T59S	probably damaging	Het
Gm7353	A	T	7: 3,160,168 (GRCm39)		noncoding transcript	Het
Grep1	G	A	17: 23,936,966 (GRCm39)	P72S	probably damaging	Het
H2-T23	G	T	17: 36,342,496 (GRCm39)		probably benign	Het
Ighv1-16	T	A	12: 114,629,531 (GRCm39)	R85S	probably benign	Het
Ipcef1	T	A	10: 6,840,569 (GRCm39)	D376V	probably damaging	Het
Kdm1a	T	A	4: 136,300,381 (GRCm39)		probably null	Het
Klk1b27	A	T	7: 43,705,088 (GRCm39)	Q85L	probably benign	Het
Krt39	A	T	11: 99,411,971 (GRCm39)	D38E	probably benign	Het
L1td1	T	C	4: 98,621,999 (GRCm39)	L187P	possibly damaging	Het
Lrrc8b	C	T	5: 105,629,208 (GRCm39)	S518L	probably benign	Het
Lyg1	C	T	1: 37,986,034 (GRCm39)	D176N	probably damaging	Het
Mctp1	A	G	13: 76,836,678 (GRCm39)	N82D	probably damaging	Het
Muc6	T	C	7: 141,226,690 (GRCm39)		probably benign	Het
Nynrin	T	C	14: 56,108,088 (GRCm39)	L1065P	probably damaging	Het
Or5b118	T	C	19: 13,449,260 (GRCm39)	S309P	probably benign	Het
Pcdh7	T	A	5: 57,879,567 (GRCm39)	S1041T	probably damaging	Het
Pkd1l1	T	A	11: 8,811,302 (GRCm39)	M1518L	probably benign	Het
Prl8a6	G	T	13: 27,619,453 (GRCm39)	Q90K	probably benign	Het
Ptcd1	T	A	5: 145,096,475 (GRCm39)	D206V	probably damaging	Het
Rap1gap	C	A	4: 137,447,688 (GRCm39)	D478E	probably benign	Het
Scn5a	A	G	9: 119,330,732 (GRCm39)	Y1269H	probably damaging	Het
Sdc2	A	T	15: 33,028,290 (GRCm39)	H136L	probably benign	Het
Senp6	A	T	9: 80,033,715 (GRCm39)	I120F	probably damaging	Het
Shisa5	G	A	9: 108,885,162 (GRCm39)		probably null	Het
Slc19a1	A	G	10: 76,878,103 (GRCm39)	S213G	probably null	Het
Smim8	TTTAATGAAGAGCT	TT	4: 34,771,261 (GRCm39)		probably benign	Het
Tbc1d20	T	A	2: 152,150,245 (GRCm39)		probably null	Het
Tll2	T	C	19: 41,093,373 (GRCm39)	H481R	probably benign	Het
Tmc7	A	G	7: 118,138,663 (GRCm39)	V692A	probably benign	Het
Tmem234	T	C	4: 129,500,924 (GRCm39)		probably null	Het
Vmn1r237	C	G	17: 21,535,069 (GRCm39)	T264S	probably benign	Het
Vmn2r98	A	T	17: 19,286,260 (GRCm39)	T253S	probably benign	Het
Zfyve27	T	C	19: 42,171,102 (GRCm39)	Y191H	probably damaging	Het

Other mutations in Gpr153

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01350:Gpr153	APN	4	152,366,423 (GRCm39)	unclassified	probably benign
IGL01368:Gpr153	APN	4	152,367,451 (GRCm39)	missense	probably benign	0.40
IGL01568:Gpr153	APN	4	152,366,825 (GRCm39)	splice site	probably null
IGL01672:Gpr153	APN	4	152,364,370 (GRCm39)	nonsense	probably null
R0735:Gpr153	UTSW	4	152,363,830 (GRCm39)	nonsense	probably null
R0925:Gpr153	UTSW	4	152,366,331 (GRCm39)	missense	probably benign
R1302:Gpr153	UTSW	4	152,364,400 (GRCm39)	missense	probably damaging	1.00
R1829:Gpr153	UTSW	4	152,366,849 (GRCm39)	missense	possibly damaging	0.70
R2041:Gpr153	UTSW	4	152,367,810 (GRCm39)	missense	probably benign
R4698:Gpr153	UTSW	4	152,366,240 (GRCm39)	missense	probably damaging	1.00
R5069:Gpr153	UTSW	4	152,364,340 (GRCm39)	missense	probably damaging	0.99
R5623:Gpr153	UTSW	4	152,366,398 (GRCm39)	missense	possibly damaging	0.89
R5940:Gpr153	UTSW	4	152,367,832 (GRCm39)	missense	probably benign	0.12
R6773:Gpr153	UTSW	4	152,363,757 (GRCm39)	missense	probably damaging	1.00
R6944:Gpr153	UTSW	4	152,363,820 (GRCm39)	missense	probably damaging	1.00
R7486:Gpr153	UTSW	4	152,366,858 (GRCm39)	missense	probably benign	0.01
R8170:Gpr153	UTSW	4	152,364,634 (GRCm39)	missense	probably damaging	1.00
R8699:Gpr153	UTSW	4	152,363,558 (GRCm39)	start gained	probably benign
R8701:Gpr153	UTSW	4	152,363,558 (GRCm39)	start gained	probably benign
R8732:Gpr153	UTSW	4	152,363,558 (GRCm39)	start gained	probably benign
R9047:Gpr153	UTSW	4	152,364,664 (GRCm39)	missense	probably damaging	1.00
R9383:Gpr153	UTSW	4	152,367,516 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GATGGTGTCCTTCATCCTGTCG -3'
(R):5'- AGAAGCCACATCCCTGAGAG -3'

Sequencing Primer
(F):5'- GGCCCTTCCTGCCGTTG -3'
(R):5'- CCCATGAGGCAGTCATAGATG -3'

Posted On

2016-12-15