Mutagenetix > Incidental Mutation

Incidental Mutation 'R5812:Ankrd11'

447558

Institutional Source

Beutler Lab

Gene Symbol

Ankrd11

Ensembl Gene

ENSMUSG00000035569

Gene Name

ankyrin repeat domain 11

Synonyms

Yod, 3010027A04Rik, 2410104C19Rik, 9530048I21Rik

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5812 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

123610561-123769016 bp(-) (GRCm39)

Type of Mutation

splice site (3215 bp from exon)

DNA Base Change (assembly)

A to T at 123620544 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098333] [ENSMUST00000098334] [ENSMUST00000127664] [ENSMUST00000172906]

AlphaFold

E9Q4F7

Predicted Effect

probably damaging
Transcript: ENSMUST00000098333
AA Change: F1103I

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000095938
Gene: ENSMUSG00000035569
AA Change: F1103I

Domain	Start	End	E-Value	Type
ANK	188	217	2.58e-3	SMART
ANK	221	250	1.31e-4	SMART
ANK	254	283	5.04e-6	SMART
low complexity region	311	324	N/A	INTRINSIC
low complexity region	366	377	N/A	INTRINSIC
low complexity region	429	440	N/A	INTRINSIC
low complexity region	470	487	N/A	INTRINSIC
low complexity region	499	521	N/A	INTRINSIC
low complexity region	534	549	N/A	INTRINSIC
low complexity region	596	609	N/A	INTRINSIC
low complexity region	649	669	N/A	INTRINSIC
coiled coil region	786	826	N/A	INTRINSIC
low complexity region	867	877	N/A	INTRINSIC
low complexity region	965	984	N/A	INTRINSIC
low complexity region	1040	1055	N/A	INTRINSIC
low complexity region	1058	1082	N/A	INTRINSIC
low complexity region	1187	1200	N/A	INTRINSIC
low complexity region	1204	1227	N/A	INTRINSIC
low complexity region	1268	1289	N/A	INTRINSIC
low complexity region	1294	1306	N/A	INTRINSIC
coiled coil region	1374	1406	N/A	INTRINSIC
low complexity region	1476	1496	N/A	INTRINSIC
low complexity region	1508	1523	N/A	INTRINSIC
low complexity region	1526	1547	N/A	INTRINSIC
coiled coil region	1598	1625	N/A	INTRINSIC
low complexity region	1770	1781	N/A	INTRINSIC
low complexity region	1874	1885	N/A	INTRINSIC
low complexity region	1913	1922	N/A	INTRINSIC
low complexity region	1969	1979	N/A	INTRINSIC
low complexity region	2035	2045	N/A	INTRINSIC
low complexity region	2057	2071	N/A	INTRINSIC
low complexity region	2162	2179	N/A	INTRINSIC
low complexity region	2191	2209	N/A	INTRINSIC
low complexity region	2224	2236	N/A	INTRINSIC
low complexity region	2250	2263	N/A	INTRINSIC
low complexity region	2294	2305	N/A	INTRINSIC
low complexity region	2391	2409	N/A	INTRINSIC
low complexity region	2445	2455	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000098334
AA Change: F1082I

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000095939
Gene: ENSMUSG00000035569
AA Change: F1082I

Domain	Start	End	E-Value	Type
ANK	167	196	2.58e-3	SMART
ANK	200	229	1.31e-4	SMART
ANK	233	262	5.04e-6	SMART
low complexity region	290	303	N/A	INTRINSIC
low complexity region	345	356	N/A	INTRINSIC
low complexity region	408	419	N/A	INTRINSIC
low complexity region	449	466	N/A	INTRINSIC
low complexity region	478	500	N/A	INTRINSIC
low complexity region	513	528	N/A	INTRINSIC
low complexity region	575	588	N/A	INTRINSIC
low complexity region	628	648	N/A	INTRINSIC
coiled coil region	765	805	N/A	INTRINSIC
low complexity region	846	856	N/A	INTRINSIC
low complexity region	944	963	N/A	INTRINSIC
low complexity region	1019	1034	N/A	INTRINSIC
low complexity region	1037	1061	N/A	INTRINSIC
low complexity region	1166	1179	N/A	INTRINSIC
low complexity region	1183	1206	N/A	INTRINSIC
low complexity region	1247	1268	N/A	INTRINSIC
low complexity region	1273	1285	N/A	INTRINSIC
coiled coil region	1353	1385	N/A	INTRINSIC
low complexity region	1455	1475	N/A	INTRINSIC
low complexity region	1487	1502	N/A	INTRINSIC
low complexity region	1505	1526	N/A	INTRINSIC
coiled coil region	1577	1604	N/A	INTRINSIC
low complexity region	1749	1760	N/A	INTRINSIC
low complexity region	1853	1864	N/A	INTRINSIC
low complexity region	1892	1901	N/A	INTRINSIC
low complexity region	1948	1958	N/A	INTRINSIC
low complexity region	2014	2024	N/A	INTRINSIC
low complexity region	2036	2050	N/A	INTRINSIC
low complexity region	2141	2158	N/A	INTRINSIC
low complexity region	2170	2188	N/A	INTRINSIC
low complexity region	2203	2215	N/A	INTRINSIC
low complexity region	2229	2242	N/A	INTRINSIC
low complexity region	2273	2284	N/A	INTRINSIC
low complexity region	2370	2388	N/A	INTRINSIC
low complexity region	2424	2434	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000127664

SMART Domains

Protein: ENSMUSP00000118564
Gene: ENSMUSG00000092329

Domain	Start	End	E-Value	Type
Pfam:Glycos_transf_2	104	287	7.4e-31	PFAM
Pfam:Glyco_transf_7C	261	331	4.9e-8	PFAM
RICIN	406	531	9.28e-27	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000172906

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211940

Predicted Effect

probably null
Transcript: ENSMUST00000212050

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212337

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212728

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This locus encodes an ankryin repeat domain-containing protein. The encoded protein inhibits ligand-dependent activation of transcription. Mutations in this gene have been associated with KBG syndrome, which is characterized by macrodontia, distinctive craniofacial features, short stature, skeletal anomalies, global developmental delay, seizures and intellectual disability. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 2 and X. [provided by RefSeq, Jan 2012]
PHENOTYPE: Mice homozygous for a spontaneous allele die by E9.5, are small and fail to turn. Mice heterozygous for a spontaneous allele exhibit craniofacial abnormalities, decreased weight, osteoporosis and osteopenia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579F01Rik	A	G	3: 137,882,299 (GRCm39)	S9P	probably damaging	Het
Aadacl2fm2	T	A	3: 59,654,693 (GRCm39)	Y176N	probably damaging	Het
Acsl5	G	A	19: 55,283,268 (GRCm39)	V615I	probably benign	Het
Ankk1	T	C	9: 49,338,153 (GRCm39)	K47E	probably benign	Het
Bcl9l	T	C	9: 44,417,941 (GRCm39)	V593A	probably benign	Het
Cadps	T	C	14: 12,376,685 (GRCm38)	I1271V	probably benign	Het
Ccdc125	T	C	13: 100,820,812 (GRCm39)	W152R	probably damaging	Het
Cep192	T	C	18: 67,984,808 (GRCm39)	V1606A	possibly damaging	Het
Csgalnact1	T	C	8: 68,854,036 (GRCm39)	N255S	probably benign	Het
Cxxc5	T	A	18: 35,992,109 (GRCm39)	V170E	probably damaging	Het
Defb6	C	T	8: 19,278,110 (GRCm39)	R61C	possibly damaging	Het
Dnah10	T	A	5: 124,824,810 (GRCm39)	N655K	probably benign	Het
Fry	G	A	5: 150,323,136 (GRCm39)	A1096T	probably damaging	Het
Gli1	C	T	10: 127,173,284 (GRCm39)	G125S	probably damaging	Het
Igkv14-126	A	T	6: 67,873,424 (GRCm39)	I51F	possibly damaging	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Jtb	T	C	3: 90,141,284 (GRCm39)	S87P	probably benign	Het
Kdm6b	A	T	11: 69,296,755 (GRCm39)	I504N	probably damaging	Het
Lin9	T	A	1: 180,496,763 (GRCm39)	L351I	probably benign	Het
Map2k4	A	G	11: 65,626,031 (GRCm39)	I136T	probably damaging	Het
Marchf4	T	C	1: 72,468,076 (GRCm39)	T319A	probably benign	Het
Nr2e3	TCCATCGGAGTGTTCCC	TC	9: 59,850,701 (GRCm39)		probably benign	Het
Nt5e	T	C	9: 88,251,108 (GRCm39)	V459A	probably damaging	Het
Ogdhl	A	G	14: 32,054,822 (GRCm39)	K257E	probably damaging	Het
Or1e16	AGCGGTCGTAGGC	AGC	11: 73,286,480 (GRCm39)		probably null	Het
Or2g25	A	G	17: 37,970,630 (GRCm39)	L198P	probably damaging	Het
Osmr	A	T	15: 6,866,540 (GRCm39)	V378D	probably damaging	Het
Pcdhb18	G	A	18: 37,623,537 (GRCm39)	R289Q	probably benign	Het
Pdzd7	G	T	19: 45,025,310 (GRCm39)	T395K	probably damaging	Het
Rasgef1c	A	G	11: 49,847,970 (GRCm39)	D35G	probably benign	Het
Rbm19	T	C	5: 120,279,642 (GRCm39)	F770L	probably damaging	Het
Rit2	C	A	18: 31,108,514 (GRCm39)	C157F	probably damaging	Het
Slc22a22	A	G	15: 57,119,869 (GRCm39)		probably null	Het
Slc8b1	T	C	5: 120,651,403 (GRCm39)		probably null	Het
Speer2	C	A	16: 69,655,783 (GRCm39)	R14S	possibly damaging	Het
Tas2r105	C	A	6: 131,663,836 (GRCm39)	L197F	possibly damaging	Het
Tmprss11b	T	A	5: 86,812,957 (GRCm39)	H113L	possibly damaging	Het
Ttbk2	G	A	2: 120,653,040 (GRCm39)	P64S	probably damaging	Het
Urb1	A	T	16: 90,601,425 (GRCm39)	H115Q	probably damaging	Het
Vps13c	T	A	9: 67,889,777 (GRCm39)		probably benign	Het
Zbtb39	T	A	10: 127,577,429 (GRCm39)	M1K	probably null	Het

Other mutations in Ankrd11

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00949:Ankrd11	APN	8	123,635,467 (GRCm39)	missense	possibly damaging	0.59
IGL00971:Ankrd11	APN	8	123,622,092 (GRCm39)	missense	probably damaging	1.00
IGL01017:Ankrd11	APN	8	123,621,467 (GRCm39)	missense	probably damaging	1.00
IGL01137:Ankrd11	APN	8	123,611,075 (GRCm39)	missense	probably damaging	0.99
IGL01659:Ankrd11	APN	8	123,622,110 (GRCm39)	missense	probably damaging	1.00
IGL01920:Ankrd11	APN	8	123,642,636 (GRCm39)	splice site	probably benign
IGL01964:Ankrd11	APN	8	123,616,475 (GRCm39)	missense	probably damaging	0.97
IGL02131:Ankrd11	APN	8	123,621,149 (GRCm39)	missense	probably damaging	1.00
IGL02226:Ankrd11	APN	8	123,618,984 (GRCm39)	missense	probably damaging	1.00
IGL02549:Ankrd11	APN	8	123,618,032 (GRCm39)	missense	probably damaging	1.00
IGL02642:Ankrd11	APN	8	123,617,390 (GRCm39)	missense	probably damaging	1.00
IGL02643:Ankrd11	APN	8	123,619,061 (GRCm39)	missense	probably damaging	0.98
IGL02861:Ankrd11	APN	8	123,622,566 (GRCm39)	missense	probably damaging	0.99
IGL03086:Ankrd11	APN	8	123,621,249 (GRCm39)	missense	probably damaging	1.00
IGL03336:Ankrd11	APN	8	123,618,582 (GRCm39)	missense	probably benign	0.00
anchors	UTSW	8	123,622,509 (GRCm39)	missense	probably damaging	0.99
away	UTSW	8	123,618,692 (GRCm39)	missense	probably damaging	1.00
bluebell	UTSW	8	123,618,524 (GRCm39)	missense	probably damaging	0.97
Navy	UTSW	8	123,635,473 (GRCm39)	nonsense	probably null
BB001:Ankrd11	UTSW	8	123,622,641 (GRCm39)	missense	possibly damaging	0.95
BB011:Ankrd11	UTSW	8	123,622,641 (GRCm39)	missense	possibly damaging	0.95
R0051:Ankrd11	UTSW	8	123,616,481 (GRCm39)	missense	probably damaging	1.00
R0051:Ankrd11	UTSW	8	123,616,481 (GRCm39)	missense	probably damaging	1.00
R0110:Ankrd11	UTSW	8	123,618,914 (GRCm39)	missense	possibly damaging	0.95
R0281:Ankrd11	UTSW	8	123,622,307 (GRCm39)	missense	probably benign	0.01
R0450:Ankrd11	UTSW	8	123,618,914 (GRCm39)	missense	possibly damaging	0.95
R0481:Ankrd11	UTSW	8	123,626,775 (GRCm39)	missense	probably damaging	1.00
R0542:Ankrd11	UTSW	8	123,622,509 (GRCm39)	missense	probably damaging	0.99
R0606:Ankrd11	UTSW	8	123,619,571 (GRCm39)	missense	probably benign	0.04
R0702:Ankrd11	UTSW	8	123,616,505 (GRCm39)	missense	probably damaging	1.00
R0730:Ankrd11	UTSW	8	123,618,692 (GRCm39)	missense	probably damaging	1.00
R0737:Ankrd11	UTSW	8	123,622,575 (GRCm39)	missense	probably damaging	0.99
R1401:Ankrd11	UTSW	8	123,619,789 (GRCm39)	missense	probably benign	0.23
R1464:Ankrd11	UTSW	8	123,619,463 (GRCm39)	missense	probably damaging	1.00
R1464:Ankrd11	UTSW	8	123,619,463 (GRCm39)	missense	probably damaging	1.00
R1470:Ankrd11	UTSW	8	123,626,463 (GRCm39)	missense	probably damaging	0.98
R1470:Ankrd11	UTSW	8	123,626,463 (GRCm39)	missense	probably damaging	0.98
R1641:Ankrd11	UTSW	8	123,618,485 (GRCm39)	missense	probably benign	0.03
R1950:Ankrd11	UTSW	8	123,616,608 (GRCm39)	missense	probably damaging	1.00
R2004:Ankrd11	UTSW	8	123,629,161 (GRCm39)	critical splice donor site	probably null
R2401:Ankrd11	UTSW	8	123,635,473 (GRCm39)	nonsense	probably null
R2425:Ankrd11	UTSW	8	123,619,902 (GRCm39)	missense	possibly damaging	0.86
R2830:Ankrd11	UTSW	8	123,618,935 (GRCm39)	missense	probably damaging	1.00
R2910:Ankrd11	UTSW	8	123,635,537 (GRCm39)	missense	probably damaging	1.00
R2911:Ankrd11	UTSW	8	123,635,537 (GRCm39)	missense	probably damaging	1.00
R3736:Ankrd11	UTSW	8	123,618,524 (GRCm39)	missense	probably damaging	0.97
R3738:Ankrd11	UTSW	8	123,623,454 (GRCm39)	unclassified	probably benign
R3739:Ankrd11	UTSW	8	123,623,454 (GRCm39)	unclassified	probably benign
R3813:Ankrd11	UTSW	8	123,618,117 (GRCm39)	missense	probably benign
R4012:Ankrd11	UTSW	8	123,619,156 (GRCm39)	missense	probably damaging	0.98
R4183:Ankrd11	UTSW	8	123,626,415 (GRCm39)	missense	possibly damaging	0.88
R4213:Ankrd11	UTSW	8	123,617,765 (GRCm39)	missense	probably benign	0.00
R4469:Ankrd11	UTSW	8	123,623,326 (GRCm39)	missense	probably damaging	1.00
R4482:Ankrd11	UTSW	8	123,620,228 (GRCm39)	missense	probably damaging	1.00
R4935:Ankrd11	UTSW	8	123,626,922 (GRCm39)	missense	probably benign	0.02
R4940:Ankrd11	UTSW	8	123,616,560 (GRCm39)	missense	probably damaging	1.00
R5145:Ankrd11	UTSW	8	123,617,943 (GRCm39)	utr 3 prime	probably benign
R5154:Ankrd11	UTSW	8	123,619,878 (GRCm39)	missense	probably damaging	1.00
R5230:Ankrd11	UTSW	8	123,617,216 (GRCm39)	missense	probably benign	0.11
R5283:Ankrd11	UTSW	8	123,610,921 (GRCm39)	missense	probably damaging	1.00
R5377:Ankrd11	UTSW	8	123,620,453 (GRCm39)	splice site	probably null
R5513:Ankrd11	UTSW	8	123,619,259 (GRCm39)	missense	probably benign	0.38
R5518:Ankrd11	UTSW	8	123,617,733 (GRCm39)	missense	possibly damaging	0.93
R5549:Ankrd11	UTSW	8	123,617,117 (GRCm39)	missense	probably benign	0.02
R5579:Ankrd11	UTSW	8	123,610,970 (GRCm39)	missense	probably damaging	0.97
R5595:Ankrd11	UTSW	8	123,621,043 (GRCm39)	nonsense	probably null
R5650:Ankrd11	UTSW	8	123,614,136 (GRCm39)	missense	probably damaging	0.99
R5717:Ankrd11	UTSW	8	123,619,377 (GRCm39)	missense	possibly damaging	0.92
R5753:Ankrd11	UTSW	8	123,622,043 (GRCm39)	missense	possibly damaging	0.90
R5782:Ankrd11	UTSW	8	123,626,756 (GRCm39)	missense	probably damaging	1.00
R5823:Ankrd11	UTSW	8	123,622,529 (GRCm39)	missense	probably benign	0.12
R5900:Ankrd11	UTSW	8	123,617,805 (GRCm39)	missense	probably benign	0.00
R5975:Ankrd11	UTSW	8	123,616,488 (GRCm39)	missense	possibly damaging	0.93
R5979:Ankrd11	UTSW	8	123,619,139 (GRCm39)	missense	probably damaging	1.00
R6000:Ankrd11	UTSW	8	123,617,934 (GRCm39)	missense	possibly damaging	0.73
R6145:Ankrd11	UTSW	8	123,619,400 (GRCm39)	missense	probably damaging	1.00
R6252:Ankrd11	UTSW	8	123,620,561 (GRCm39)	missense	possibly damaging	0.87
R6302:Ankrd11	UTSW	8	123,616,728 (GRCm39)	missense	probably benign
R6457:Ankrd11	UTSW	8	123,635,503 (GRCm39)	missense	probably damaging	1.00
R6513:Ankrd11	UTSW	8	123,616,919 (GRCm39)	missense	probably benign	0.02
R6582:Ankrd11	UTSW	8	123,618,368 (GRCm39)	missense	probably benign	0.00
R6738:Ankrd11	UTSW	8	123,618,660 (GRCm39)	missense	probably damaging	0.99
R6865:Ankrd11	UTSW	8	123,621,683 (GRCm39)	missense	probably benign	0.41
R6913:Ankrd11	UTSW	8	123,621,650 (GRCm39)	missense	probably benign	0.01
R7101:Ankrd11	UTSW	8	123,622,194 (GRCm39)	missense	probably benign	0.35
R7116:Ankrd11	UTSW	8	123,622,869 (GRCm39)	missense	probably damaging	1.00
R7477:Ankrd11	UTSW	8	123,621,124 (GRCm39)	missense	possibly damaging	0.91
R7534:Ankrd11	UTSW	8	123,621,149 (GRCm39)	missense	probably damaging	1.00
R7555:Ankrd11	UTSW	8	123,614,145 (GRCm39)	missense	probably damaging	0.99
R7627:Ankrd11	UTSW	8	123,617,690 (GRCm39)	missense	possibly damaging	0.63
R7658:Ankrd11	UTSW	8	123,620,403 (GRCm39)	missense	probably benign
R7721:Ankrd11	UTSW	8	123,621,498 (GRCm39)	missense	probably damaging	1.00
R7731:Ankrd11	UTSW	8	123,622,172 (GRCm39)	missense	probably benign	0.12
R7792:Ankrd11	UTSW	8	123,610,970 (GRCm39)	missense	probably damaging	0.97
R7924:Ankrd11	UTSW	8	123,622,641 (GRCm39)	missense	possibly damaging	0.95
R7939:Ankrd11	UTSW	8	123,617,812 (GRCm39)	missense	probably damaging	1.00
R8022:Ankrd11	UTSW	8	123,614,332 (GRCm39)	missense	probably damaging	1.00
R8222:Ankrd11	UTSW	8	123,622,347 (GRCm39)	missense	probably damaging	0.98
R8362:Ankrd11	UTSW	8	123,618,797 (GRCm39)	missense	probably damaging	0.96
R8430:Ankrd11	UTSW	8	123,620,105 (GRCm39)	missense	probably benign	0.01
R8511:Ankrd11	UTSW	8	123,626,468 (GRCm39)	missense
R8726:Ankrd11	UTSW	8	123,620,765 (GRCm39)	missense	possibly damaging	0.90
R8888:Ankrd11	UTSW	8	123,621,014 (GRCm39)	missense	possibly damaging	0.87
R8895:Ankrd11	UTSW	8	123,621,014 (GRCm39)	missense	possibly damaging	0.87
R8928:Ankrd11	UTSW	8	123,622,718 (GRCm39)	missense	probably damaging	0.99
R8930:Ankrd11	UTSW	8	123,622,718 (GRCm39)	missense	probably damaging	0.99
R8931:Ankrd11	UTSW	8	123,622,718 (GRCm39)	missense	probably damaging	0.99
R8936:Ankrd11	UTSW	8	123,621,840 (GRCm39)	missense	possibly damaging	0.69
R9018:Ankrd11	UTSW	8	123,622,251 (GRCm39)	missense	probably damaging	1.00
R9113:Ankrd11	UTSW	8	123,614,072 (GRCm39)	missense	possibly damaging	0.60
R9399:Ankrd11	UTSW	8	123,618,179 (GRCm39)	missense	probably benign
R9644:Ankrd11	UTSW	8	123,617,682 (GRCm39)	missense	probably benign	0.00
R9645:Ankrd11	UTSW	8	123,617,682 (GRCm39)	missense	probably benign	0.00
R9647:Ankrd11	UTSW	8	123,617,682 (GRCm39)	missense	probably benign	0.00
R9683:Ankrd11	UTSW	8	123,617,682 (GRCm39)	missense	probably benign	0.00
RF019:Ankrd11	UTSW	8	123,623,373 (GRCm39)	missense	probably damaging	1.00
Z1176:Ankrd11	UTSW	8	123,622,542 (GRCm39)	missense	possibly damaging	0.68
Z1177:Ankrd11	UTSW	8	123,626,881 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGTGCTGCCTGTCAGAAGAG -3'
(R):5'- TGACTTCCCCAAGAACCTGG -3'

Sequencing Primer
(F):5'- AAGAGGACTTCCGGTGCCTG -3'
(R):5'- AAGAACCTGGAGCCCTGG -3'

Posted On

2016-12-15