Mutagenetix > Incidental Mutation

Incidental Mutation 'R5805:Vmn2r15'

448442

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r15

Ensembl Gene

ENSMUSG00000091375

Gene Name

vomeronasal 2, receptor 15

Synonyms

EG211223

MMRRC Submission

043212-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R5805 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109434135-109445422 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109434806 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 633 (I633V)

Ref Sequence

ENSEMBL: ENSMUSP00000128333 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167133]

AlphaFold

L7N2A0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000167133
AA Change: I633V

PolyPhen 2 Score 0.880 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000128333
Gene: ENSMUSG00000091375
AA Change: I633V

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	75	472	1e-29	PFAM
Pfam:NCD3G	514	568	5.8e-18	PFAM
Pfam:7tm_3	601	836	9.1e-55	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca5	C	T	11: 110,170,216 (GRCm39)	C1293Y	probably benign	Het
Adam8	T	A	7: 139,565,794 (GRCm39)	D611V	probably damaging	Het
Arhgap33	T	C	7: 30,225,839 (GRCm39)	T576A	probably benign	Het
Atf7	A	T	15: 102,466,022 (GRCm39)		probably null	Het
Baiap3	T	A	17: 25,466,489 (GRCm39)	T464S	probably benign	Het
Ccdc40	T	C	11: 119,136,906 (GRCm39)		probably null	Het
Celf2	T	C	2: 6,558,598 (GRCm39)	E430G	probably damaging	Het
Chia1	A	G	3: 106,035,792 (GRCm39)	T211A	probably damaging	Het
Ciz1	T	C	2: 32,257,408 (GRCm39)	F151S	probably damaging	Het
Dnm2	A	G	9: 21,378,965 (GRCm39)	T175A	probably damaging	Het
Doc2b	A	G	11: 75,663,364 (GRCm39)	S363P	probably damaging	Het
Garem1	C	T	18: 21,281,492 (GRCm39)	R288H	probably benign	Het
Gpr132	G	A	12: 112,816,416 (GRCm39)	R137C	probably damaging	Het
Helz2	C	A	2: 180,882,301 (GRCm39)	C164F	probably damaging	Het
Itfg1	A	T	8: 86,493,601 (GRCm39)	S293T	probably benign	Het
Kcnn2	T	A	18: 45,816,198 (GRCm39)	D336E	probably damaging	Het
Kifc2	C	T	15: 76,546,353 (GRCm39)	A245V	probably benign	Het
Krt18	T	A	15: 101,939,735 (GRCm39)	I311N	probably benign	Het
Lypla1	T	C	1: 4,900,517 (GRCm39)	M7T	possibly damaging	Het
Mef2a	T	C	7: 66,901,416 (GRCm39)	M285V	possibly damaging	Het
Micu1	A	C	10: 59,663,128 (GRCm39)	K353Q	possibly damaging	Het
Mpv17l	A	G	16: 13,760,013 (GRCm39)		probably benign	Het
Ntrk1	G	A	3: 87,687,479 (GRCm39)	R652W	probably damaging	Het
Or13a28	T	A	7: 140,218,384 (GRCm39)	F257I	probably benign	Het
Or13c7	T	C	4: 43,855,152 (GRCm39)	I281T	probably benign	Het
Or4c107	G	A	2: 88,788,985 (GRCm39)	M58I	possibly damaging	Het
Pcdh15	C	A	10: 74,066,091 (GRCm39)	T252K	probably damaging	Het
Pcsk5	T	C	19: 17,434,193 (GRCm39)	M1392V	probably benign	Het
Phf20	T	A	2: 156,149,214 (GRCm39)	V964E	probably damaging	Het
Pira13	G	A	7: 3,825,622 (GRCm39)	L416F	probably benign	Het
Plcg2	T	C	8: 118,325,234 (GRCm39)		probably null	Het
Rnf10	A	T	5: 115,382,127 (GRCm39)	C693S	probably benign	Het
Rnf19b	A	G	4: 128,952,617 (GRCm39)	Y185C	probably damaging	Het
Ros1	G	T	10: 51,999,385 (GRCm39)	D1167E	probably damaging	Het
Sidt2	G	A	9: 45,853,497 (GRCm39)	S701L	probably damaging	Het
Slc66a2	C	T	18: 80,306,658 (GRCm39)	P76L	probably damaging	Het
Spag1	T	C	15: 36,200,430 (GRCm39)	I345T	probably damaging	Het
Srcap	T	A	7: 127,141,211 (GRCm39)	S1603T	possibly damaging	Het
Stag1	T	A	9: 100,678,831 (GRCm39)	Y251N	probably damaging	Het
Stxbp5	T	C	10: 9,776,330 (GRCm39)	N33S	probably benign	Het
Tnrc6a	T	C	7: 122,769,299 (GRCm39)	L363P	probably damaging	Het
U2surp	C	T	9: 95,361,357 (GRCm39)	R591H	possibly damaging	Het
Usf3	A	G	16: 44,041,109 (GRCm39)	N1863S	possibly damaging	Het
Zfp979	T	C	4: 147,698,067 (GRCm39)	D214G	probably damaging	Het

Other mutations in Vmn2r15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01335:Vmn2r15	APN	5	109,434,602 (GRCm39)	missense	possibly damaging	0.70
IGL01367:Vmn2r15	APN	5	109,441,075 (GRCm39)	missense	probably damaging	0.99
IGL01844:Vmn2r15	APN	5	109,434,135 (GRCm39)	makesense	probably null
IGL02190:Vmn2r15	APN	5	109,441,240 (GRCm39)	missense	probably damaging	1.00
IGL02754:Vmn2r15	APN	5	109,441,134 (GRCm39)	nonsense	probably null
IGL02797:Vmn2r15	APN	5	109,445,250 (GRCm39)	missense	probably benign	0.18
IGL03301:Vmn2r15	APN	5	109,445,221 (GRCm39)	critical splice donor site	probably null
IGL03407:Vmn2r15	APN	5	109,434,185 (GRCm39)	nonsense	probably null
BB001:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
BB011:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r15	UTSW	5	109,435,008 (GRCm39)	missense	probably damaging	0.99
PIT4520001:Vmn2r15	UTSW	5	109,434,871 (GRCm39)	missense	probably damaging	1.00
R0038:Vmn2r15	UTSW	5	109,441,010 (GRCm39)	missense	possibly damaging	0.46
R0111:Vmn2r15	UTSW	5	109,435,022 (GRCm39)	missense	possibly damaging	0.56
R0379:Vmn2r15	UTSW	5	109,434,344 (GRCm39)	missense	probably damaging	1.00
R0427:Vmn2r15	UTSW	5	109,434,953 (GRCm39)	missense	probably damaging	1.00
R0639:Vmn2r15	UTSW	5	109,440,881 (GRCm39)	missense	probably benign	0.22
R0964:Vmn2r15	UTSW	5	109,445,401 (GRCm39)	missense	probably benign	0.34
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1232:Vmn2r15	UTSW	5	109,441,168 (GRCm39)	missense	probably benign	0.39
R1241:Vmn2r15	UTSW	5	109,440,770 (GRCm39)	missense	probably damaging	1.00
R1244:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R1394:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1395:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1423:Vmn2r15	UTSW	5	109,441,093 (GRCm39)	missense	probably damaging	1.00
R1439:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	probably damaging	1.00
R1513:Vmn2r15	UTSW	5	109,441,195 (GRCm39)	missense	probably damaging	1.00
R1777:Vmn2r15	UTSW	5	109,442,136 (GRCm39)	missense	possibly damaging	0.79
R1844:Vmn2r15	UTSW	5	109,434,860 (GRCm39)	nonsense	probably null
R2072:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2074:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2122:Vmn2r15	UTSW	5	109,434,322 (GRCm39)	missense	probably damaging	1.00
R2208:Vmn2r15	UTSW	5	109,445,309 (GRCm39)	missense	possibly damaging	0.64
R2268:Vmn2r15	UTSW	5	109,441,073 (GRCm39)	missense	probably benign	0.31
R2831:Vmn2r15	UTSW	5	109,434,458 (GRCm39)	missense	probably damaging	1.00
R3848:Vmn2r15	UTSW	5	109,445,312 (GRCm39)	missense	probably benign	0.00
R4058:Vmn2r15	UTSW	5	109,441,312 (GRCm39)	missense	probably damaging	0.99
R4615:Vmn2r15	UTSW	5	109,441,348 (GRCm39)	missense	possibly damaging	0.91
R4663:Vmn2r15	UTSW	5	109,441,940 (GRCm39)	missense	probably benign
R4681:Vmn2r15	UTSW	5	109,434,488 (GRCm39)	missense	probably damaging	0.97
R4751:Vmn2r15	UTSW	5	109,434,620 (GRCm39)	missense	probably benign	0.01
R5095:Vmn2r15	UTSW	5	109,436,317 (GRCm39)	critical splice acceptor site	probably null
R5300:Vmn2r15	UTSW	5	109,441,974 (GRCm39)	missense	probably damaging	0.99
R5309:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R5335:Vmn2r15	UTSW	5	109,434,673 (GRCm39)	missense	probably damaging	0.99
R5421:Vmn2r15	UTSW	5	109,434,401 (GRCm39)	missense	probably damaging	1.00
R6280:Vmn2r15	UTSW	5	109,441,291 (GRCm39)	missense	possibly damaging	0.65
R6324:Vmn2r15	UTSW	5	109,434,137 (GRCm39)	makesense	probably null
R6383:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R6772:Vmn2r15	UTSW	5	109,434,238 (GRCm39)	missense	probably damaging	0.99
R6991:Vmn2r15	UTSW	5	109,441,180 (GRCm39)	missense	probably damaging	1.00
R7194:Vmn2r15	UTSW	5	109,440,649 (GRCm39)	missense	probably damaging	1.00
R7365:Vmn2r15	UTSW	5	109,445,388 (GRCm39)	missense	probably benign	0.15
R7365:Vmn2r15	UTSW	5	109,441,105 (GRCm39)	missense	probably benign	0.19
R7423:Vmn2r15	UTSW	5	109,445,394 (GRCm39)	missense	probably benign	0.00
R7552:Vmn2r15	UTSW	5	109,440,774 (GRCm39)	nonsense	probably null
R7619:Vmn2r15	UTSW	5	109,436,190 (GRCm39)	critical splice donor site	probably null
R7892:Vmn2r15	UTSW	5	109,434,217 (GRCm39)	missense	probably damaging	1.00
R7924:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
R8058:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R8099:Vmn2r15	UTSW	5	109,441,185 (GRCm39)	missense	possibly damaging	0.58
R8189:Vmn2r15	UTSW	5	109,434,713 (GRCm39)	missense	probably benign	0.02
R8275:Vmn2r15	UTSW	5	109,434,150 (GRCm39)	missense	probably benign	0.08
R8277:Vmn2r15	UTSW	5	109,441,310 (GRCm39)	missense	probably damaging	1.00
R8277:Vmn2r15	UTSW	5	109,440,970 (GRCm39)	missense	probably benign	0.00
R8465:Vmn2r15	UTSW	5	109,445,302 (GRCm39)	missense	probably damaging	1.00
R8679:Vmn2r15	UTSW	5	109,434,779 (GRCm39)	missense	probably benign	0.31
R8682:Vmn2r15	UTSW	5	109,441,938 (GRCm39)	missense	probably damaging	0.98
R8809:Vmn2r15	UTSW	5	109,434,874 (GRCm39)	missense	probably benign	0.22
R9016:Vmn2r15	UTSW	5	109,442,109 (GRCm39)	missense	probably benign	0.01
R9372:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	possibly damaging	0.60
R9596:Vmn2r15	UTSW	5	109,440,791 (GRCm39)	missense	probably benign	0.01
R9619:Vmn2r15	UTSW	5	109,440,622 (GRCm39)	missense	possibly damaging	0.58
R9685:Vmn2r15	UTSW	5	109,440,598 (GRCm39)	missense	probably benign	0.05
R9716:Vmn2r15	UTSW	5	109,445,224 (GRCm39)	missense	possibly damaging	0.88
R9772:Vmn2r15	UTSW	5	109,434,923 (GRCm39)	missense	probably damaging	1.00
X0065:Vmn2r15	UTSW	5	109,441,174 (GRCm39)	missense	probably damaging	1.00
Z1187:Vmn2r15	UTSW	5	109,434,529 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGTTAGGTGCCCCTGATGC -3'
(R):5'- TGCTAACTTAGAGAAAACCCAATGC -3'

Sequencing Primer
(F):5'- GATGCCAGCATCCCTCTCATC -3'
(R):5'- CAATGCCTCCAAAGAGTTGTGTC -3'

Posted On

2016-12-15