Incidental Mutation 'R5830:Gpr155'
| ID |
449255 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr155
|
| Ensembl Gene |
ENSMUSG00000041762 |
| Gene Name |
G protein-coupled receptor 155 |
| Synonyms |
DEPDC3, 1110017O10Rik, PGR22, F730029F15Rik |
| MMRRC Submission |
043219-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R5830 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
73171850-73216842 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 73200433 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Alanine
at position 358
(V358A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000107675
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000076463]
[ENSMUST00000112043]
[ENSMUST00000112044]
|
| AlphaFold |
A2AWR3 |
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000048504
|
| SMART Domains |
Protein: ENSMUSP00000037772
Gene: ENSMUSG00000041762
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
1.9e-16 |
PFAM |
|
Pfam:Mem_trans
|
187 |
360 |
4.9e-12 |
PFAM |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000076463
AA Change: V358A
PolyPhen 2
Score 0.820 (Sensitivity: 0.84; Specificity: 0.93)
|
| SMART Domains |
Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: V358A
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000112043
AA Change: V358A
PolyPhen 2
Score 0.820 (Sensitivity: 0.84; Specificity: 0.93)
|
| SMART Domains |
Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: V358A
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000112044
AA Change: V358A
PolyPhen 2
Score 0.926 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: V358A
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
Pfam:Mem_trans
|
187 |
360 |
8.3e-11 |
PFAM |
|
transmembrane domain
|
382 |
404 |
N/A |
INTRINSIC |
|
transmembrane domain
|
414 |
436 |
N/A |
INTRINSIC |
|
transmembrane domain
|
449 |
471 |
N/A |
INTRINSIC |
|
transmembrane domain
|
491 |
510 |
N/A |
INTRINSIC |
|
transmembrane domain
|
629 |
651 |
N/A |
INTRINSIC |
|
transmembrane domain
|
661 |
683 |
N/A |
INTRINSIC |
|
DEP
|
731 |
805 |
8.28e-12 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000129184
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000138573
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000184503
|
| Coding Region Coverage |
- 1x: 99.8%
- 3x: 99.4%
- 10x: 97.1%
- 20x: 90.6%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 34 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Apobec3 |
A |
T |
15: 79,783,268 (GRCm39) |
N115I |
possibly damaging |
Het |
| Arl14ep |
T |
C |
2: 106,799,539 (GRCm39) |
S101G |
possibly damaging |
Het |
| Atp6v0a2 |
C |
T |
5: 124,779,485 (GRCm39) |
T200I |
probably damaging |
Het |
| Ccdc40 |
C |
A |
11: 119,133,572 (GRCm39) |
H571N |
probably benign |
Het |
| Cdc42bpb |
G |
A |
12: 111,312,016 (GRCm39) |
R99* |
probably null |
Het |
| Cdyl2 |
A |
G |
8: 117,321,823 (GRCm39) |
Y235H |
probably benign |
Het |
| Chmp2a |
T |
C |
7: 12,766,039 (GRCm39) |
D161G |
probably damaging |
Het |
| Ep400 |
A |
T |
5: 110,831,862 (GRCm39) |
W2091R |
unknown |
Het |
| Epha8 |
G |
A |
4: 136,663,701 (GRCm39) |
Q452* |
probably null |
Het |
| Fbn2 |
A |
G |
18: 58,247,541 (GRCm39) |
F451S |
probably benign |
Het |
| Fndc1 |
C |
A |
17: 8,007,918 (GRCm39) |
R72L |
possibly damaging |
Het |
| Gfpt2 |
A |
G |
11: 49,699,888 (GRCm39) |
E81G |
probably benign |
Het |
| Hnrnpk |
A |
T |
13: 58,545,548 (GRCm39) |
Y92* |
probably null |
Het |
| Il34 |
A |
G |
8: 111,475,323 (GRCm39) |
V71A |
probably damaging |
Het |
| Inpp5e |
A |
G |
2: 26,290,427 (GRCm39) |
F416L |
probably damaging |
Het |
| Iqgap2 |
C |
T |
13: 95,811,880 (GRCm39) |
R707H |
probably damaging |
Het |
| Kdsr |
A |
G |
1: 106,675,262 (GRCm39) |
S125P |
possibly damaging |
Het |
| Ldb1 |
T |
A |
19: 46,022,557 (GRCm39) |
M307L |
probably benign |
Het |
| Lrr1 |
T |
A |
12: 69,225,445 (GRCm39) |
S374T |
possibly damaging |
Het |
| Mtmr2 |
A |
G |
9: 13,713,274 (GRCm39) |
I412V |
probably benign |
Het |
| Ncor1 |
T |
C |
11: 62,235,589 (GRCm39) |
I298V |
possibly damaging |
Het |
| Ndst1 |
A |
G |
18: 60,836,910 (GRCm39) |
F384L |
probably damaging |
Het |
| Nlrc5 |
A |
G |
8: 95,199,542 (GRCm39) |
E51G |
probably damaging |
Het |
| Obox6 |
G |
A |
7: 15,568,382 (GRCm39) |
H165Y |
possibly damaging |
Het |
| Or5k17 |
T |
A |
16: 58,746,457 (GRCm39) |
H159L |
possibly damaging |
Het |
| Pde8b |
A |
T |
13: 95,178,398 (GRCm39) |
F582Y |
probably benign |
Het |
| Pik3r4 |
C |
A |
9: 105,522,023 (GRCm39) |
Y196* |
probably null |
Het |
| Pink1 |
A |
T |
4: 138,043,325 (GRCm39) |
M1K |
probably null |
Het |
| Scn7a |
A |
T |
2: 66,544,395 (GRCm39) |
Y365* |
probably null |
Het |
| Sec16a |
A |
T |
2: 26,330,853 (GRCm39) |
D387E |
probably benign |
Het |
| Sel1l |
A |
T |
12: 91,799,945 (GRCm39) |
F127Y |
probably damaging |
Het |
| Syt7 |
T |
A |
19: 10,399,151 (GRCm39) |
N82K |
probably damaging |
Het |
| Zfp358 |
G |
T |
8: 3,545,846 (GRCm39) |
V143L |
probably benign |
Het |
| Zkscan2 |
T |
C |
7: 123,079,323 (GRCm39) |
N878S |
possibly damaging |
Het |
|
| Other mutations in Gpr155 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00731:Gpr155
|
APN |
2 |
73,192,957 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01432:Gpr155
|
APN |
2 |
73,182,229 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
IGL01528:Gpr155
|
APN |
2 |
73,192,767 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01718:Gpr155
|
APN |
2 |
73,212,576 (GRCm39) |
missense |
probably benign |
|
|
IGL01733:Gpr155
|
APN |
2 |
73,183,956 (GRCm39) |
splice site |
probably null |
|
|
IGL03342:Gpr155
|
APN |
2 |
73,180,022 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT1430001:Gpr155
|
UTSW |
2 |
73,200,482 (GRCm39) |
missense |
probably benign |
0.08 |
|
PIT4810001:Gpr155
|
UTSW |
2 |
73,178,607 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0226:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0399:Gpr155
|
UTSW |
2 |
73,200,346 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R0445:Gpr155
|
UTSW |
2 |
73,200,488 (GRCm39) |
splice site |
probably benign |
|
|
R1570:Gpr155
|
UTSW |
2 |
73,200,382 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1598:Gpr155
|
UTSW |
2 |
73,200,434 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1647:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1648:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1756:Gpr155
|
UTSW |
2 |
73,197,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1760:Gpr155
|
UTSW |
2 |
73,212,279 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2033:Gpr155
|
UTSW |
2 |
73,178,526 (GRCm39) |
missense |
probably benign |
0.18 |
|
R2044:Gpr155
|
UTSW |
2 |
73,203,977 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2145:Gpr155
|
UTSW |
2 |
73,187,002 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2172:Gpr155
|
UTSW |
2 |
73,212,471 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2274:Gpr155
|
UTSW |
2 |
73,178,479 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3878:Gpr155
|
UTSW |
2 |
73,198,736 (GRCm39) |
nonsense |
probably null |
|
|
R3924:Gpr155
|
UTSW |
2 |
73,200,420 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4910:Gpr155
|
UTSW |
2 |
73,197,882 (GRCm39) |
nonsense |
probably null |
|
|
R4950:Gpr155
|
UTSW |
2 |
73,212,529 (GRCm39) |
missense |
probably benign |
|
|
R5337:Gpr155
|
UTSW |
2 |
73,178,592 (GRCm39) |
missense |
probably benign |
0.32 |
|
R5887:Gpr155
|
UTSW |
2 |
73,174,062 (GRCm39) |
nonsense |
probably null |
|
|
R5929:Gpr155
|
UTSW |
2 |
73,204,011 (GRCm39) |
nonsense |
probably null |
|
|
R6293:Gpr155
|
UTSW |
2 |
73,204,341 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R6553:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6585:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7003:Gpr155
|
UTSW |
2 |
73,173,961 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7353:Gpr155
|
UTSW |
2 |
73,197,835 (GRCm39) |
nonsense |
probably null |
|
|
R7506:Gpr155
|
UTSW |
2 |
73,198,683 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R7631:Gpr155
|
UTSW |
2 |
73,213,291 (GRCm39) |
intron |
probably benign |
|
|
R7753:Gpr155
|
UTSW |
2 |
73,212,550 (GRCm39) |
missense |
probably benign |
0.27 |
|
R7810:Gpr155
|
UTSW |
2 |
73,212,296 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7813:Gpr155
|
UTSW |
2 |
73,212,329 (GRCm39) |
nonsense |
probably null |
|
|
R7815:Gpr155
|
UTSW |
2 |
73,192,904 (GRCm39) |
missense |
probably benign |
|
|
R7873:Gpr155
|
UTSW |
2 |
73,173,934 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R8506:Gpr155
|
UTSW |
2 |
73,173,806 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8680:Gpr155
|
UTSW |
2 |
73,174,039 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8856:Gpr155
|
UTSW |
2 |
73,203,993 (GRCm39) |
missense |
probably benign |
0.27 |
|
R8872:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9116:Gpr155
|
UTSW |
2 |
73,204,109 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
R9683:Gpr155
|
UTSW |
2 |
73,192,780 (GRCm39) |
missense |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- CTCCTGACTGGAAAGACATACTCAG -3'
(R):5'- TCCATGGTGTCTTCACTGGC -3'
Sequencing Primer
(F):5'- CTGGAAAGACATACTCAGTCAATTAC -3'
(R):5'- GTGTCTTCACTGGCTTTCATTTTCAG -3'
|
| Posted On |
2016-12-20 |
Incidental Mutation