Mutagenetix > Incidental Mutation

Incidental Mutation 'R5836:Vmn1r224'

449614

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r224

Ensembl Gene

ENSMUSG00000091151

Gene Name

vomeronasal 1 receptor 224

Synonyms

Gm7673

MMRRC Submission

043222-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.072) question?

Stock #

R5836 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20639425-20640321 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 20639953 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 177 (I177L)

Ref Sequence

ENSEMBL: ENSMUSP00000132957 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170076]

AlphaFold

E9PWK2

Predicted Effect

probably benign
Transcript: ENSMUST00000170076
AA Change: I177L

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000132957
Gene: ENSMUSG00000091151
AA Change: I177L

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	289	3.8e-8	PFAM
Pfam:V1R	34	290	4.3e-30	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf1	G	T	17: 36,272,918 (GRCm39)	A243D	possibly damaging	Het
Azin2	C	T	4: 128,842,670 (GRCm39)	G128D	probably damaging	Het
Babam1	A	G	8: 71,855,687 (GRCm39)	E260G	probably benign	Het
Brwd1	A	T	16: 95,865,958 (GRCm39)	S275T	probably damaging	Het
Cd8a	T	C	6: 71,350,775 (GRCm39)	V80A	possibly damaging	Het
Clec4a3	T	C	6: 122,929,861 (GRCm39)	F12S	possibly damaging	Het
Dennd2b	A	T	7: 109,140,552 (GRCm39)	S225T	possibly damaging	Het
Dnah5	A	C	15: 28,383,738 (GRCm39)	N2987H	probably damaging	Het
Dock9	A	G	14: 121,918,763 (GRCm39)	F78S	probably damaging	Het
Eml3	A	G	19: 8,918,659 (GRCm39)	T885A	possibly damaging	Het
Esr1	T	C	10: 4,662,817 (GRCm39)	V145A	probably benign	Het
Gm5108	A	G	5: 68,101,953 (GRCm39)		probably benign	Het
Gpr179	T	C	11: 97,229,882 (GRCm39)	S758G	probably benign	Het
Heatr1	T	A	13: 12,423,617 (GRCm39)	L538Q	probably damaging	Het
Ikzf2	A	G	1: 69,578,546 (GRCm39)	I176T	probably damaging	Het
Lrp3	A	G	7: 34,902,747 (GRCm39)	V533A	probably damaging	Het
Nkx2-5	A	T	17: 27,058,063 (GRCm39)	V297E	possibly damaging	Het
Or13a19	G	A	7: 139,902,827 (GRCm39)	V72I	probably benign	Het
Or2y16	T	C	11: 49,335,353 (GRCm39)	L225P	probably damaging	Het
Or8g23	T	C	9: 38,971,918 (GRCm39)	T15A	probably benign	Het
Pclo	T	A	5: 14,728,549 (GRCm39)		probably benign	Het
Pdgfra	T	C	5: 75,324,435 (GRCm39)	W97R	possibly damaging	Het
Plekha5	T	C	6: 140,372,250 (GRCm39)	Y67H	probably damaging	Het
Plin5	A	T	17: 56,422,549 (GRCm39)		probably null	Het
Pramel11	A	G	4: 143,623,490 (GRCm39)	V228A	probably benign	Het
Prickle1	T	A	15: 93,400,898 (GRCm39)	K529*	probably null	Het
Ptbp2	G	A	3: 119,519,746 (GRCm39)	T107I	probably damaging	Het
Ptpn12	C	T	5: 21,214,544 (GRCm39)	W197*	probably null	Het
Rhobtb1	T	C	10: 69,105,819 (GRCm39)	V128A	probably damaging	Het
Ryr2	T	C	13: 11,618,618 (GRCm39)	T3866A	probably damaging	Het
Serpina3m	T	C	12: 104,355,509 (GRCm39)	Y59H	probably damaging	Het
Slc12a6	T	C	2: 112,172,343 (GRCm39)	V414A	possibly damaging	Het
Slc34a1	T	C	13: 55,561,278 (GRCm39)	M581T	probably benign	Het
Slco1c1	A	G	6: 141,515,040 (GRCm39)	Y596C	probably damaging	Het
Stoml1	T	C	9: 58,168,123 (GRCm39)	L278P	probably benign	Het
Tecpr2	C	T	12: 110,897,945 (GRCm39)	A399V	possibly damaging	Het
Tmem229a	T	C	6: 24,955,016 (GRCm39)	E246G	probably damaging	Het
Zswim5	C	T	4: 116,842,000 (GRCm39)	T860I	probably benign	Het

Other mutations in Vmn1r224

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1638:Vmn1r224	UTSW	17	20,639,587 (GRCm39)	missense	probably benign
R1783:Vmn1r224	UTSW	17	20,639,447 (GRCm39)	missense	probably benign	0.33
R2032:Vmn1r224	UTSW	17	20,639,658 (GRCm39)	missense	probably benign	0.21
R2090:Vmn1r224	UTSW	17	20,639,524 (GRCm39)	missense	probably benign	0.42
R2993:Vmn1r224	UTSW	17	20,639,472 (GRCm39)	missense	probably damaging	0.97
R4235:Vmn1r224	UTSW	17	20,639,624 (GRCm39)	missense	possibly damaging	0.79
R4749:Vmn1r224	UTSW	17	20,640,013 (GRCm39)	missense	probably benign	0.00
R4762:Vmn1r224	UTSW	17	20,639,902 (GRCm39)	missense	possibly damaging	0.63
R6080:Vmn1r224	UTSW	17	20,639,818 (GRCm39)	missense	possibly damaging	0.68
R6294:Vmn1r224	UTSW	17	20,640,083 (GRCm39)	missense	probably benign
R6303:Vmn1r224	UTSW	17	20,640,028 (GRCm39)	missense	possibly damaging	0.90
R7030:Vmn1r224	UTSW	17	20,639,789 (GRCm39)	missense	probably benign	0.35
R7605:Vmn1r224	UTSW	17	20,640,221 (GRCm39)	nonsense	probably null
R7903:Vmn1r224	UTSW	17	20,640,309 (GRCm39)	missense	probably benign	0.12
R8399:Vmn1r224	UTSW	17	20,640,011 (GRCm39)	missense	probably damaging	1.00
R9028:Vmn1r224	UTSW	17	20,640,112 (GRCm39)	missense	possibly damaging	0.76
R9130:Vmn1r224	UTSW	17	20,640,242 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn1r224	UTSW	17	20,640,136 (GRCm39)	missense	probably benign	0.14

Predicted Primers

PCR Primer
(F):5'- TCACCATCAGTCCTAGGACCTC -3'
(R):5'- GATACCAAGGCCAGTATCCTG -3'

Sequencing Primer
(F):5'- AGTCCTAGGACCTCTTTTTCAATGAG -3'
(R):5'- CAAGGCCAGTATCCTGTGGGTAG -3'

Posted On

2016-12-20