Mutagenetix > Incidental Mutation

Incidental Mutation 'R5697:Akap9'

450727

Institutional Source

Beutler Lab

Gene Symbol

Akap9

Ensembl Gene

ENSMUSG00000040407

Gene Name

A kinase anchor protein 9

Synonyms

mei2-5, repro12, AKAP450, G1-448-15, 5730481H23Rik

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.436) question?

Stock #

R5697 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

3977410-4130204 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 4010170 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 309 (L309P)

Ref Sequence

ENSEMBL: ENSMUSP00000117209 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044492] [ENSMUST00000143365]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000044492
AA Change: L291P

PolyPhen 2 Score 0.918 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000046129
Gene: ENSMUSG00000040407
AA Change: L291P

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	98	115	N/A	INTRINSIC
Blast:HPT	126	197	6e-21	BLAST
low complexity region	237	249	N/A	INTRINSIC
low complexity region	297	315	N/A	INTRINSIC
coiled coil region	404	593	N/A	INTRINSIC
coiled coil region	622	756	N/A	INTRINSIC
coiled coil region	777	843	N/A	INTRINSIC
coiled coil region	888	958	N/A	INTRINSIC
low complexity region	982	997	N/A	INTRINSIC
coiled coil region	1037	1065	N/A	INTRINSIC
low complexity region	1233	1246	N/A	INTRINSIC
internal_repeat_2	1247	1312	7.75e-5	PROSPERO
internal_repeat_1	1377	1485	2.63e-5	PROSPERO
coiled coil region	1522	1589	N/A	INTRINSIC
coiled coil region	1789	2107	N/A	INTRINSIC
coiled coil region	2132	2318	N/A	INTRINSIC
internal_repeat_1	2322	2445	2.63e-5	PROSPERO
coiled coil region	2455	2494	N/A	INTRINSIC
low complexity region	2587	2598	N/A	INTRINSIC
low complexity region	2627	2640	N/A	INTRINSIC
internal_repeat_2	2934	2997	7.75e-5	PROSPERO
low complexity region	3000	3016	N/A	INTRINSIC
coiled coil region	3109	3307	N/A	INTRINSIC
coiled coil region	3455	3493	N/A	INTRINSIC
coiled coil region	3521	3556	N/A	INTRINSIC
Pfam:PACT_coil_coil	3576	3657	1.2e-27	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000140977

Predicted Effect

possibly damaging
Transcript: ENSMUST00000143365
AA Change: L309P

PolyPhen 2 Score 0.918 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000117209
Gene: ENSMUSG00000040407
AA Change: L309P

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	122	133	N/A	INTRINSIC
Blast:HPT	144	215	9e-22	BLAST
low complexity region	255	267	N/A	INTRINSIC
low complexity region	315	333	N/A	INTRINSIC
coiled coil region	422	571	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177448

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a chemically induced allele exhibit male infertily with abnormal spermatogenesis and Sertoli maturation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr8	T	C	14: 29,713,630 (GRCm39)	F550S	possibly damaging	Het
Adam34l	A	T	8: 44,079,616 (GRCm39)	W203R	probably damaging	Het
Adamts15	G	A	9: 30,823,090 (GRCm39)	T326I	probably damaging	Het
Arfgef1	T	C	1: 10,231,063 (GRCm39)	M1149V	probably benign	Het
Atp6v0a4	C	T	6: 38,027,442 (GRCm39)		probably null	Het
Capn9	C	T	8: 125,315,810 (GRCm39)	H39Y	unknown	Het
Ccn2	A	G	10: 24,473,354 (GRCm39)	T298A	probably benign	Het
Celsr2	G	A	3: 108,311,237 (GRCm39)	Q1425*	probably null	Het
Chd6	A	G	2: 160,859,971 (GRCm39)	L610P	probably damaging	Het
Clasp2	A	T	9: 113,689,190 (GRCm39)	T424S	probably benign	Het
Clec5a	T	C	6: 40,559,204 (GRCm39)	D35G	probably benign	Het
Dnhd1	T	G	7: 105,323,395 (GRCm39)	V599G	probably damaging	Het
Enpep	T	C	3: 129,102,772 (GRCm39)	T395A	probably benign	Het
Evc2	G	T	5: 37,527,952 (GRCm39)	L320F	probably damaging	Het
H2bc22	C	T	13: 21,971,961 (GRCm39)		probably null	Het
Hivep3	C	T	4: 119,954,152 (GRCm39)	H823Y	possibly damaging	Het
Ighv1-59	C	T	12: 115,298,968 (GRCm39)	E29K	possibly damaging	Het
Itsn1	A	G	16: 91,598,477 (GRCm39)	I137V	possibly damaging	Het
Kntc1	A	G	5: 123,903,070 (GRCm39)	T316A	probably benign	Het
Map4k5	T	C	12: 69,877,210 (GRCm39)	T312A	probably benign	Het
Megf6	G	T	4: 154,342,686 (GRCm39)	A642S	probably null	Het
Mrgprb3	G	T	7: 48,292,673 (GRCm39)	L293M	probably damaging	Het
Nes	A	G	3: 87,885,155 (GRCm39)	E1138G	probably damaging	Het
Niban1	T	C	1: 151,576,012 (GRCm39)	F379L	probably damaging	Het
Pias2	A	G	18: 77,220,884 (GRCm39)	K373R	probably damaging	Het
Prss23	A	G	7: 89,159,190 (GRCm39)	F293S	probably damaging	Het
Pzp	A	G	6: 128,502,152 (GRCm39)	Y66H	probably benign	Het
Rnf213	A	G	11: 119,374,720 (GRCm39)	R5061G	possibly damaging	Het
Sergef	A	G	7: 46,288,683 (GRCm39)		probably benign	Het
Slc7a1	A	G	5: 148,270,792 (GRCm39)	V558A	probably benign	Het
Smc2	A	G	4: 52,459,045 (GRCm39)	E480G	probably benign	Het
Smyd3	T	G	1: 179,239,247 (GRCm39)	K111T	probably damaging	Het
Tchh	A	T	3: 93,352,350 (GRCm39)	R597*	probably null	Het
Ttc6	G	T	12: 57,724,000 (GRCm39)	V1043L	probably benign	Het
Vmn1r10	T	G	6: 57,090,474 (GRCm39)	L22R	probably damaging	Het
Vmn2r56	T	C	7: 12,449,917 (GRCm39)	D107G	probably damaging	Het
Zfp1005	C	T	2: 150,111,394 (GRCm39)	H695Y	possibly damaging	Het
Zfp365	A	G	10: 67,745,470 (GRCm39)	Y103H	probably benign	Het
Zfp39	G	T	11: 58,780,661 (GRCm39)	H700Q	probably benign	Het
Zfp982	A	T	4: 147,597,046 (GRCm39)	E134D	probably benign	Het

Other mutations in Akap9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00478:Akap9	APN	5	4,096,639 (GRCm39)	missense	probably damaging	0.97
IGL00642:Akap9	APN	5	4,010,842 (GRCm39)	missense	probably damaging	0.99
IGL00786:Akap9	APN	5	4,120,522 (GRCm39)	missense	probably damaging	1.00
IGL00788:Akap9	APN	5	4,110,480 (GRCm39)	missense	probably damaging	1.00
IGL00969:Akap9	APN	5	4,051,550 (GRCm39)	missense	probably benign
IGL01014:Akap9	APN	5	4,018,683 (GRCm39)	missense	probably benign	0.41
IGL01302:Akap9	APN	5	4,020,711 (GRCm39)	missense	probably benign	0.27
IGL01610:Akap9	APN	5	4,082,839 (GRCm39)	missense	possibly damaging	0.95
IGL01620:Akap9	APN	5	4,010,218 (GRCm39)	missense	probably benign	0.11
IGL01862:Akap9	APN	5	4,115,856 (GRCm39)	missense	probably damaging	0.99
IGL01862:Akap9	APN	5	4,001,705 (GRCm39)	missense	probably damaging	0.99
IGL02151:Akap9	APN	5	4,082,728 (GRCm39)	nonsense	probably null
IGL02635:Akap9	APN	5	4,120,500 (GRCm39)	missense	possibly damaging	0.59
IGL02858:Akap9	APN	5	4,119,130 (GRCm39)	missense	possibly damaging	0.88
IGL02967:Akap9	APN	5	4,026,164 (GRCm39)	missense	probably benign	0.07
IGL03064:Akap9	APN	5	4,018,755 (GRCm39)	missense	probably damaging	1.00
IGL03289:Akap9	APN	5	4,127,261 (GRCm39)	missense	probably damaging	1.00
Andy	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
blimey	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
hoarder	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
marinarum	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
miser	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
naviculus	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
thrifty	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
wee_one	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
FR4449:Akap9	UTSW	5	4,031,214 (GRCm39)	unclassified	probably benign
PIT1430001:Akap9	UTSW	5	4,079,849 (GRCm39)	missense	probably damaging	1.00
PIT4366001:Akap9	UTSW	5	4,096,221 (GRCm39)	missense	probably benign	0.24
R0088:Akap9	UTSW	5	4,011,946 (GRCm39)	missense	probably benign	0.22
R0309:Akap9	UTSW	5	4,119,038 (GRCm39)	missense	probably benign	0.01
R0387:Akap9	UTSW	5	4,001,678 (GRCm39)	splice site	probably benign
R0440:Akap9	UTSW	5	4,114,569 (GRCm39)	missense	probably damaging	0.99
R0441:Akap9	UTSW	5	4,011,714 (GRCm39)	missense	probably benign	0.15
R0491:Akap9	UTSW	5	4,022,851 (GRCm39)	unclassified	probably benign
R0501:Akap9	UTSW	5	4,020,685 (GRCm39)	missense	probably damaging	1.00
R0507:Akap9	UTSW	5	4,119,043 (GRCm39)	missense	probably benign	0.41
R0544:Akap9	UTSW	5	4,119,185 (GRCm39)	missense	probably benign	0.22
R0581:Akap9	UTSW	5	4,100,620 (GRCm39)	missense	probably benign	0.03
R0611:Akap9	UTSW	5	4,004,870 (GRCm39)	missense	probably benign	0.00
R0620:Akap9	UTSW	5	4,114,136 (GRCm39)	missense	probably damaging	0.98
R0639:Akap9	UTSW	5	4,110,318 (GRCm39)	missense	probably damaging	1.00
R0932:Akap9	UTSW	5	4,096,492 (GRCm39)	missense	possibly damaging	0.77
R0944:Akap9	UTSW	5	4,114,742 (GRCm39)	splice site	probably null
R1101:Akap9	UTSW	5	4,096,205 (GRCm39)	missense	probably benign	0.00
R1159:Akap9	UTSW	5	4,010,865 (GRCm39)	missense	probably damaging	0.98
R1170:Akap9	UTSW	5	4,105,671 (GRCm39)	missense	probably benign
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,998,783 (GRCm39)	missense	probably benign	0.13
R1453:Akap9	UTSW	5	4,025,614 (GRCm39)	splice site	probably null
R1551:Akap9	UTSW	5	4,119,174 (GRCm39)	missense	probably benign	0.02
R1608:Akap9	UTSW	5	4,011,783 (GRCm39)	missense	probably damaging	1.00
R1652:Akap9	UTSW	5	4,127,210 (GRCm39)	missense	probably damaging	1.00
R1659:Akap9	UTSW	5	4,114,633 (GRCm39)	missense	probably damaging	1.00
R1713:Akap9	UTSW	5	4,089,345 (GRCm39)	critical splice donor site	probably null
R1719:Akap9	UTSW	5	4,007,645 (GRCm39)	nonsense	probably null
R1720:Akap9	UTSW	5	4,022,791 (GRCm39)	missense	possibly damaging	0.63
R1757:Akap9	UTSW	5	4,051,667 (GRCm39)	missense	probably benign	0.41
R1872:Akap9	UTSW	5	4,051,406 (GRCm39)	missense	probably damaging	1.00
R1876:Akap9	UTSW	5	4,011,809 (GRCm39)	missense	probably benign	0.28
R1881:Akap9	UTSW	5	4,100,173 (GRCm39)	missense	probably benign
R1950:Akap9	UTSW	5	4,010,677 (GRCm39)	missense	probably damaging	1.00
R1980:Akap9	UTSW	5	4,022,771 (GRCm39)	missense	probably damaging	0.99
R1993:Akap9	UTSW	5	4,088,520 (GRCm39)	splice site	probably null
R2008:Akap9	UTSW	5	4,010,131 (GRCm39)	missense	possibly damaging	0.47
R2020:Akap9	UTSW	5	4,011,967 (GRCm39)	missense	probably damaging	1.00
R2051:Akap9	UTSW	5	4,025,685 (GRCm39)	nonsense	probably null
R2061:Akap9	UTSW	5	4,011,010 (GRCm39)	missense	probably damaging	1.00
R2109:Akap9	UTSW	5	4,094,847 (GRCm39)	missense	possibly damaging	0.47
R2135:Akap9	UTSW	5	4,114,509 (GRCm39)	missense	probably damaging	1.00
R2225:Akap9	UTSW	5	4,127,271 (GRCm39)	missense	probably damaging	0.96
R2232:Akap9	UTSW	5	4,096,603 (GRCm39)	missense	probably damaging	1.00
R2424:Akap9	UTSW	5	4,115,279 (GRCm39)	missense	probably damaging	0.97
R2483:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R2879:Akap9	UTSW	5	4,026,353 (GRCm39)	intron	probably benign
R3622:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3623:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3624:Akap9	UTSW	5	4,026,235 (GRCm39)	missense	possibly damaging	0.65
R3722:Akap9	UTSW	5	4,120,351 (GRCm39)	missense	probably damaging	1.00
R3806:Akap9	UTSW	5	4,004,410 (GRCm39)	missense	probably benign	0.00
R3919:Akap9	UTSW	5	4,011,764 (GRCm39)	nonsense	probably null
R4023:Akap9	UTSW	5	4,042,077 (GRCm39)	missense	possibly damaging	0.66
R4093:Akap9	UTSW	5	4,093,996 (GRCm39)	missense	probably damaging	0.99
R4434:Akap9	UTSW	5	4,082,708 (GRCm39)	missense	probably damaging	0.99
R4529:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4530:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4532:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4533:Akap9	UTSW	5	4,093,948 (GRCm39)	missense	probably damaging	1.00
R4585:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4586:Akap9	UTSW	5	4,026,151 (GRCm39)	missense	probably benign	0.00
R4655:Akap9	UTSW	5	4,096,403 (GRCm39)	missense	probably benign	0.14
R4676:Akap9	UTSW	5	4,114,515 (GRCm39)	nonsense	probably null
R4676:Akap9	UTSW	5	4,082,774 (GRCm39)	missense	probably damaging	1.00
R4724:Akap9	UTSW	5	4,105,339 (GRCm39)	missense	probably benign
R4731:Akap9	UTSW	5	4,012,266 (GRCm39)	missense	possibly damaging	0.54
R4732:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4733:Akap9	UTSW	5	4,063,901 (GRCm39)	missense	probably damaging	0.98
R4743:Akap9	UTSW	5	4,011,013 (GRCm39)	missense	probably damaging	1.00
R4749:Akap9	UTSW	5	4,018,737 (GRCm39)	missense	probably benign	0.41
R4756:Akap9	UTSW	5	4,051,418 (GRCm39)	missense	probably damaging	0.99
R4757:Akap9	UTSW	5	4,058,382 (GRCm39)	missense	probably damaging	1.00
R4860:Akap9	UTSW	5	4,084,916 (GRCm39)	intron	probably benign
R4937:Akap9	UTSW	5	4,100,145 (GRCm39)	splice site	probably null
R4960:Akap9	UTSW	5	4,007,664 (GRCm39)	missense	probably benign	0.15
R4974:Akap9	UTSW	5	4,011,466 (GRCm39)	missense	possibly damaging	0.81
R5101:Akap9	UTSW	5	4,051,748 (GRCm39)	missense	probably damaging	0.96
R5160:Akap9	UTSW	5	4,080,007 (GRCm39)	missense	probably damaging	1.00
R5200:Akap9	UTSW	5	4,010,734 (GRCm39)	missense	probably benign	0.00
R5245:Akap9	UTSW	5	4,026,209 (GRCm39)	missense	probably damaging	0.99
R5293:Akap9	UTSW	5	3,998,687 (GRCm39)	missense	probably damaging	0.99
R5408:Akap9	UTSW	5	4,108,458 (GRCm39)	missense	possibly damaging	0.84
R5507:Akap9	UTSW	5	4,018,683 (GRCm39)	missense	probably benign	0.41
R5517:Akap9	UTSW	5	4,051,665 (GRCm39)	missense	possibly damaging	0.76
R5579:Akap9	UTSW	5	4,114,714 (GRCm39)	missense	possibly damaging	0.93
R5619:Akap9	UTSW	5	4,004,760 (GRCm39)	intron	probably benign
R5645:Akap9	UTSW	5	4,100,590 (GRCm39)	missense	probably benign	0.09
R5669:Akap9	UTSW	5	4,100,540 (GRCm39)	nonsense	probably null
R5686:Akap9	UTSW	5	4,021,926 (GRCm39)	missense	probably benign	0.00
R5821:Akap9	UTSW	5	4,096,064 (GRCm39)	missense	probably benign	0.13
R5875:Akap9	UTSW	5	4,127,285 (GRCm39)	missense	probably benign	0.01
R5897:Akap9	UTSW	5	4,127,904 (GRCm39)	missense	probably benign	0.23
R5999:Akap9	UTSW	5	4,093,925 (GRCm39)	missense	probably damaging	1.00
R6025:Akap9	UTSW	5	4,082,801 (GRCm39)	missense	probably damaging	1.00
R6078:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6138:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
R6225:Akap9	UTSW	5	4,012,105 (GRCm39)	missense	probably damaging	1.00
R6243:Akap9	UTSW	5	4,115,000 (GRCm39)	splice site	probably null
R6326:Akap9	UTSW	5	4,012,061 (GRCm39)	missense	probably damaging	1.00
R6564:Akap9	UTSW	5	4,078,491 (GRCm39)	missense	probably damaging	0.98
R6617:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6625:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R6632:Akap9	UTSW	5	4,063,842 (GRCm39)	splice site	probably null
R6677:Akap9	UTSW	5	4,079,869 (GRCm39)	missense	probably benign	0.21
R6717:Akap9	UTSW	5	4,114,086 (GRCm39)	missense	probably damaging	1.00
R6893:Akap9	UTSW	5	4,011,709 (GRCm39)	missense	probably benign	0.32
R6915:Akap9	UTSW	5	4,010,551 (GRCm39)	missense	probably benign	0.03
R6938:Akap9	UTSW	5	4,096,628 (GRCm39)	missense	possibly damaging	0.91
R6972:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6973:Akap9	UTSW	5	4,096,699 (GRCm39)	missense	possibly damaging	0.62
R6993:Akap9	UTSW	5	4,115,866 (GRCm39)	missense	possibly damaging	0.65
R7032:Akap9	UTSW	5	4,004,896 (GRCm39)	missense	probably benign
R7164:Akap9	UTSW	5	4,110,364 (GRCm39)	missense	probably damaging	0.96
R7170:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7192:Akap9	UTSW	5	4,055,723 (GRCm39)	splice site	probably null
R7284:Akap9	UTSW	5	4,006,246 (GRCm39)	missense	probably damaging	1.00
R7299:Akap9	UTSW	5	4,082,696 (GRCm39)	missense	probably damaging	1.00
R7313:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7326:Akap9	UTSW	5	4,095,930 (GRCm39)	missense	possibly damaging	0.47
R7343:Akap9	UTSW	5	4,096,364 (GRCm39)	missense	probably damaging	0.99
R7455:Akap9	UTSW	5	4,022,792 (GRCm39)	missense	probably benign	0.03
R7482:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7489:Akap9	UTSW	5	4,054,933 (GRCm39)	missense	probably damaging	1.00
R7525:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7528:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7576:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7577:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7578:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7610:Akap9	UTSW	5	4,007,677 (GRCm39)	missense	possibly damaging	0.95
R7658:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R7754:Akap9	UTSW	5	4,096,736 (GRCm39)	missense	probably benign	0.03
R7818:Akap9	UTSW	5	4,063,875 (GRCm39)	nonsense	probably null
R7979:Akap9	UTSW	5	4,100,381 (GRCm39)	missense	probably benign
R7991:Akap9	UTSW	5	4,114,949 (GRCm39)	splice site	probably null
R8036:Akap9	UTSW	5	4,120,397 (GRCm39)	nonsense	probably null
R8054:Akap9	UTSW	5	4,088,707 (GRCm39)	critical splice donor site	probably null
R8116:Akap9	UTSW	5	4,111,183 (GRCm39)	missense	probably benign	0.04
R8150:Akap9	UTSW	5	4,011,982 (GRCm39)	missense	probably damaging	1.00
R8234:Akap9	UTSW	5	4,094,845 (GRCm39)	missense	probably benign	0.18
R8348:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8365:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8366:Akap9	UTSW	5	4,018,745 (GRCm39)	missense	probably benign	0.04
R8448:Akap9	UTSW	5	3,998,897 (GRCm39)	critical splice donor site	probably null
R8466:Akap9	UTSW	5	4,088,659 (GRCm39)	missense	probably damaging	1.00
R8772:Akap9	UTSW	5	4,096,255 (GRCm39)	missense	probably damaging	1.00
R8881:Akap9	UTSW	5	4,011,279 (GRCm39)	missense
R8937:Akap9	UTSW	5	4,094,048 (GRCm39)	missense	possibly damaging	0.78
R8956:Akap9	UTSW	5	3,998,805 (GRCm39)	missense	possibly damaging	0.79
R9000:Akap9	UTSW	5	4,105,650 (GRCm39)	missense	probably benign
R9049:Akap9	UTSW	5	4,114,597 (GRCm39)	missense
R9074:Akap9	UTSW	5	4,127,959 (GRCm39)	missense	probably benign	0.40
R9124:Akap9	UTSW	5	4,111,284 (GRCm39)	missense	probably damaging	0.99
R9129:Akap9	UTSW	5	4,119,089 (GRCm39)	missense	probably benign	0.00
R9371:Akap9	UTSW	5	4,011,852 (GRCm39)	missense	possibly damaging	0.83
R9424:Akap9	UTSW	5	4,012,224 (GRCm39)	nonsense	probably null
R9424:Akap9	UTSW	5	4,012,223 (GRCm39)	nonsense	probably null
R9509:Akap9	UTSW	5	4,096,349 (GRCm39)	missense	probably benign
R9515:Akap9	UTSW	5	4,105,709 (GRCm39)	missense	probably damaging	1.00
R9567:Akap9	UTSW	5	4,127,311 (GRCm39)	missense	possibly damaging	0.89
R9587:Akap9	UTSW	5	4,119,149 (GRCm39)	missense	probably damaging	1.00
R9619:Akap9	UTSW	5	4,094,833 (GRCm39)	missense	probably damaging	1.00
R9635:Akap9	UTSW	5	4,100,545 (GRCm39)	missense	probably benign	0.20
R9680:Akap9	UTSW	5	4,011,587 (GRCm39)	missense	probably benign	0.03
R9691:Akap9	UTSW	5	4,010,491 (GRCm39)	missense	probably damaging	1.00
R9726:Akap9	UTSW	5	4,053,757 (GRCm39)	missense	probably benign	0.39
U15987:Akap9	UTSW	5	4,117,924 (GRCm39)	critical splice donor site	probably null
X0026:Akap9	UTSW	5	4,064,039 (GRCm39)	missense	probably damaging	1.00
X0057:Akap9	UTSW	5	4,025,598 (GRCm39)	critical splice acceptor site	probably null
Z1176:Akap9	UTSW	5	4,012,251 (GRCm39)	missense	probably damaging	0.96
Z1177:Akap9	UTSW	5	4,096,189 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGAAGGTCAGAGCTGTTGTC -3'
(R):5'- GAACACTTCCGCTCGTTCTG -3'

Sequencing Primer
(F):5'- GTCATCAGTATCTGCATACACAAG -3'
(R):5'- CTGCTGAGAGTTGCCCAGTTC -3'

Posted On

2017-01-03