Incidental Mutation 'R5703:Btd'
| ID |
451809 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Btd
|
| Ensembl Gene |
ENSMUSG00000021900 |
| Gene Name |
biotinidase |
| Synonyms |
|
| MMRRC Submission |
043183-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.148)
|
| Stock # |
R5703 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
14 |
| Chromosomal Location |
31363014-31390154 bp(+) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
C to T
at 31389004 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Stop codon
at position 242
(R242*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000087608
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000090147]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably null
Transcript: ENSMUST00000090147
AA Change: R242*
|
| SMART Domains |
Protein: ENSMUSP00000087608
Gene: ENSMUSG00000021900
AA Change: R242*
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
34 |
N/A |
INTRINSIC |
|
Pfam:CN_hydrolase
|
63 |
287 |
3.9e-17 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000128014
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.1%
- 20x: 95.0%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit behavioral/neurological defects, weakness, bone loss, weight loss, and alopecia when fed a biotin-deprived diet. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 37 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adgrb3 |
T |
A |
1: 25,459,640 (GRCm39) |
N605I |
probably damaging |
Het |
| Cdc42bpg |
A |
G |
19: 6,372,703 (GRCm39) |
D1502G |
possibly damaging |
Het |
| Chst2 |
A |
G |
9: 95,286,985 (GRCm39) |
F454L |
probably damaging |
Het |
| Col16a1 |
G |
A |
4: 129,947,092 (GRCm39) |
A146T |
probably damaging |
Het |
| Cyp3a11 |
A |
T |
5: 145,797,183 (GRCm39) |
S399T |
probably benign |
Het |
| Dspp |
C |
T |
5: 104,324,917 (GRCm39) |
H427Y |
possibly damaging |
Het |
| Dtx4 |
A |
T |
19: 12,459,574 (GRCm39) |
M410K |
possibly damaging |
Het |
| Ecpas |
A |
T |
4: 58,877,171 (GRCm39) |
|
probably null |
Het |
| Epb41l2 |
C |
T |
10: 25,317,665 (GRCm39) |
R61W |
probably damaging |
Het |
| Gbp6 |
T |
C |
5: 105,421,147 (GRCm39) |
K553E |
probably benign |
Het |
| Git1 |
CCG |
C |
11: 77,395,494 (GRCm39) |
|
probably null |
Het |
| Gm13199 |
C |
T |
2: 5,867,259 (GRCm39) |
|
probably benign |
Het |
| Gramd2a |
G |
A |
9: 59,615,299 (GRCm39) |
G13R |
probably benign |
Het |
| Hoxa2 |
G |
T |
6: 52,140,243 (GRCm39) |
Q248K |
probably damaging |
Het |
| Hycc1 |
A |
T |
5: 24,185,577 (GRCm39) |
|
probably null |
Het |
| Krt33b |
T |
C |
11: 99,916,374 (GRCm39) |
T228A |
probably benign |
Het |
| Loxhd1 |
A |
G |
18: 77,444,573 (GRCm39) |
E324G |
probably damaging |
Het |
| Map3k20 |
A |
G |
2: 72,232,514 (GRCm39) |
N390S |
probably benign |
Het |
| Mroh7 |
A |
G |
4: 106,565,757 (GRCm39) |
Y126H |
possibly damaging |
Het |
| Muc4 |
G |
A |
16: 32,555,059 (GRCm39) |
W15* |
probably null |
Het |
| Ndrg2 |
T |
A |
14: 52,147,579 (GRCm39) |
|
probably null |
Het |
| Ntsr1 |
T |
C |
2: 180,142,226 (GRCm39) |
S6P |
probably damaging |
Het |
| Or5m10 |
T |
C |
2: 85,717,783 (GRCm39) |
I213T |
probably benign |
Het |
| Or5p70 |
G |
A |
7: 107,994,707 (GRCm39) |
V127I |
probably benign |
Het |
| Pcdhb6 |
A |
T |
18: 37,467,753 (GRCm39) |
T225S |
probably benign |
Het |
| Rfng |
C |
A |
11: 120,672,842 (GRCm39) |
V294L |
probably benign |
Het |
| Scml4 |
C |
T |
10: 42,741,566 (GRCm39) |
|
probably benign |
Het |
| Slc5a2 |
A |
G |
7: 127,869,787 (GRCm39) |
I407V |
possibly damaging |
Het |
| Strc |
T |
C |
2: 121,201,295 (GRCm39) |
T1267A |
probably benign |
Het |
| Tanc1 |
T |
C |
2: 59,626,341 (GRCm39) |
V566A |
probably damaging |
Het |
| Tas1r2 |
T |
C |
4: 139,394,647 (GRCm39) |
S468P |
probably damaging |
Het |
| Tenm2 |
T |
A |
11: 35,914,626 (GRCm39) |
T2304S |
probably benign |
Het |
| Tirap |
A |
T |
9: 35,100,054 (GRCm39) |
L210Q |
probably damaging |
Het |
| Vav3 |
A |
T |
3: 109,248,557 (GRCm39) |
Q68L |
probably benign |
Het |
| Vmn2r54 |
C |
T |
7: 12,363,594 (GRCm39) |
S433N |
probably benign |
Het |
| Wars1 |
A |
T |
12: 108,841,047 (GRCm39) |
Y244N |
probably damaging |
Het |
| Zc3h6 |
A |
G |
2: 128,835,372 (GRCm39) |
|
probably benign |
Het |
|
| Other mutations in Btd |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01125:Btd
|
APN |
14 |
31,389,733 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02728:Btd
|
APN |
14 |
31,389,319 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02965:Btd
|
APN |
14 |
31,389,193 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1503:Btd
|
UTSW |
14 |
31,389,612 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1662:Btd
|
UTSW |
14 |
31,388,747 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1817:Btd
|
UTSW |
14 |
31,384,246 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R1868:Btd
|
UTSW |
14 |
31,389,266 (GRCm39) |
missense |
probably benign |
0.13 |
|
R2225:Btd
|
UTSW |
14 |
31,389,017 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2418:Btd
|
UTSW |
14 |
31,363,093 (GRCm39) |
critical splice donor site |
probably null |
|
|
R4660:Btd
|
UTSW |
14 |
31,389,760 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4727:Btd
|
UTSW |
14 |
31,384,278 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4923:Btd
|
UTSW |
14 |
31,384,044 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R5806:Btd
|
UTSW |
14 |
31,389,469 (GRCm39) |
missense |
probably benign |
|
|
R6110:Btd
|
UTSW |
14 |
31,363,065 (GRCm39) |
unclassified |
probably benign |
|
|
R6119:Btd
|
UTSW |
14 |
31,363,065 (GRCm39) |
unclassified |
probably benign |
|
|
R6120:Btd
|
UTSW |
14 |
31,363,065 (GRCm39) |
unclassified |
probably benign |
|
|
R7019:Btd
|
UTSW |
14 |
31,389,063 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R7019:Btd
|
UTSW |
14 |
31,389,062 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7021:Btd
|
UTSW |
14 |
31,389,788 (GRCm39) |
missense |
probably benign |
|
|
R7837:Btd
|
UTSW |
14 |
31,388,784 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R8176:Btd
|
UTSW |
14 |
31,384,073 (GRCm39) |
missense |
probably benign |
0.14 |
|
R8249:Btd
|
UTSW |
14 |
31,387,905 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8516:Btd
|
UTSW |
14 |
31,388,824 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9098:Btd
|
UTSW |
14 |
31,384,233 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9465:Btd
|
UTSW |
14 |
31,389,643 (GRCm39) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- GTGTTCAGCGACAATGGAACTC -3'
(R):5'- GCAATTATAAGGTGACCTTTGGGG -3'
Sequencing Primer
(F):5'- AATGGAACTCTTGTCGACCG -3'
(R):5'- CCATGTCATGGTACCAAAAGGACTTG -3'
|
| Posted On |
2017-01-03 |
Incidental Mutation