Mutagenetix > Incidental Mutation

Incidental Mutation 'R5864:Or10a3m'

454065

Institutional Source

Beutler Lab

Gene Symbol

Or10a3m

Ensembl Gene

ENSMUSG00000056946

Gene Name

olfactory receptor family 10 subfamily A member 3M

Synonyms

Olfr512, MOR268-3, GA_x6K02T2PBJ9-11043421-11044365

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.115) question?

Stock #

R5864 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

108312562-108313542 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 108312671 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 25 (D25G)

Ref Sequence

ENSEMBL: ENSMUSP00000147972 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074730] [ENSMUST00000209620]

AlphaFold

Q8VFZ4

Predicted Effect

probably benign
Transcript: ENSMUST00000074730
AA Change: D37G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000074291
Gene: ENSMUSG00000056946
AA Change: D37G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	43	320	7.8e-62	PFAM
Pfam:7TM_GPCR_Srsx	47	317	2.5e-6	PFAM
Pfam:7tm_1	53	302	2.3e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000209620
AA Change: D25G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot4	A	T	12: 84,090,178 (GRCm39)	I292F	probably benign	Het
Adamdec1	A	G	14: 68,807,551 (GRCm39)	S370P	probably damaging	Het
Ankar	T	C	1: 72,698,324 (GRCm39)	K692R	probably benign	Het
Ano6	T	C	15: 95,818,261 (GRCm39)		probably null	Het
Asns	G	A	6: 7,675,443 (GRCm39)	Q520*	probably null	Het
Bbs12	C	T	3: 37,373,639 (GRCm39)	T144I	probably damaging	Het
C8g	C	T	2: 25,388,955 (GRCm39)	G186D	probably damaging	Het
Clptm1l	T	C	13: 73,754,403 (GRCm39)	F109S	probably damaging	Het
Coa8	G	A	12: 111,717,652 (GRCm39)	V171I	probably benign	Het
Col4a1	G	A	8: 11,252,973 (GRCm39)		probably benign	Het
Cpn2	T	C	16: 30,078,501 (GRCm39)	D400G	probably damaging	Het
Dgke	G	C	11: 88,941,288 (GRCm39)	Y298*	probably null	Het
Dnah5	G	A	15: 28,297,159 (GRCm39)	R1451Q	possibly damaging	Het
Dock8	A	G	19: 25,038,584 (GRCm39)	D90G	probably damaging	Het
Dok7	A	C	5: 35,223,890 (GRCm39)	D143A	probably damaging	Het
Elk3	G	A	10: 93,120,653 (GRCm39)	A62V	probably damaging	Het
Epha2	T	A	4: 141,035,738 (GRCm39)	M58K	probably damaging	Het
Erp27	G	T	6: 136,885,098 (GRCm39)	D233E	probably benign	Het
Gm5174	T	A	10: 86,493,045 (GRCm39)		noncoding transcript	Het
Gxylt2	A	G	6: 100,760,107 (GRCm39)	D214G	probably damaging	Het
Hrob	G	A	11: 102,145,972 (GRCm39)	E83K	probably benign	Het
Ifi35	A	T	11: 101,349,069 (GRCm39)	I238F	probably damaging	Het
Ighmbp2	T	C	19: 3,311,467 (GRCm39)	T983A	probably benign	Het
Itgb4	C	T	11: 115,881,748 (GRCm39)	R766W	probably damaging	Het
Lrp1	C	A	10: 127,403,374 (GRCm39)	K2066N	possibly damaging	Het
Mansc1	T	G	6: 134,587,816 (GRCm39)		probably null	Het
Mapre3	T	C	5: 31,020,582 (GRCm39)	F101S	probably damaging	Het
Mettl8	T	C	2: 70,812,357 (GRCm39)	T58A	probably benign	Het
Mical1	G	T	10: 41,362,064 (GRCm39)	R857L	possibly damaging	Het
Nacad	T	C	11: 6,550,581 (GRCm39)	D870G	probably benign	Het
Nectin1	A	G	9: 43,702,607 (GRCm39)	D118G	probably damaging	Het
Nlrp2	A	T	7: 5,325,380 (GRCm39)	L26Q	probably damaging	Het
Pamr1	T	C	2: 102,464,693 (GRCm39)	S281P	possibly damaging	Het
Pcdhgc5	T	A	18: 37,954,814 (GRCm39)	V696E	probably damaging	Het
Pde4d	G	T	13: 110,074,582 (GRCm39)	A396S	probably benign	Het
Pecam1	G	T	11: 106,575,076 (GRCm39)	C510*	probably null	Het
Pga5	C	T	19: 10,652,513 (GRCm39)	G76S	probably damaging	Het
Phldb3	A	T	7: 24,323,571 (GRCm39)	H435L	possibly damaging	Het
Piezo1	T	C	8: 123,213,112 (GRCm39)	R1884G	possibly damaging	Het
Ripk4	T	C	16: 97,564,782 (GRCm39)	H43R	probably damaging	Het
Rtn3	A	G	19: 7,412,476 (GRCm39)	V785A	probably damaging	Het
Safb2	A	G	17: 56,873,491 (GRCm39)		probably benign	Het
Sephs1	T	C	2: 4,910,393 (GRCm39)	F288L	probably damaging	Het
Sez6l	A	G	5: 112,586,266 (GRCm39)		probably null	Het
Siglece	A	G	7: 43,308,741 (GRCm39)	L204P	probably damaging	Het
Sis	T	C	3: 72,857,151 (GRCm39)	D380G	probably damaging	Het
Slamf9	G	T	1: 172,304,033 (GRCm39)	R126L	probably benign	Het
Sorl1	A	G	9: 42,003,669 (GRCm39)	L209P	probably damaging	Het
Sptbn1	A	C	11: 30,095,925 (GRCm39)	I310S	probably damaging	Het
Tex52	A	G	6: 128,356,645 (GRCm39)	T113A	probably benign	Het
Trav6-3	T	A	14: 53,667,628 (GRCm39)	Y33*	probably null	Het
Vmn2r102	A	G	17: 19,914,943 (GRCm39)	E836G	possibly damaging	Het
Wdsub1	C	T	2: 59,708,819 (GRCm39)	C18Y	probably damaging	Het
Zfp341	C	A	2: 154,485,474 (GRCm39)	H637N	possibly damaging	Het
Zfp445	C	T	9: 122,682,552 (GRCm39)	S463N	probably benign	Het
Zfp612	G	A	8: 110,816,358 (GRCm39)	D522N	probably damaging	Het

Other mutations in Or10a3m

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01889:Or10a3m	APN	7	108,313,089 (GRCm39)	missense	probably benign	0.02
IGL01912:Or10a3m	APN	7	108,313,465 (GRCm39)	missense	possibly damaging	0.60
IGL02182:Or10a3m	APN	7	108,313,075 (GRCm39)	missense	probably benign	0.02
IGL02409:Or10a3m	APN	7	108,313,366 (GRCm39)	missense	probably benign	0.00
IGL02554:Or10a3m	APN	7	108,312,949 (GRCm39)	missense	possibly damaging	0.94
IGL03210:Or10a3m	APN	7	108,312,775 (GRCm39)	missense	probably damaging	1.00
IGL03373:Or10a3m	APN	7	108,313,339 (GRCm39)	missense	probably damaging	1.00
IGL03400:Or10a3m	APN	7	108,312,733 (GRCm39)	missense	probably benign	0.28
R0092:Or10a3m	UTSW	7	108,313,031 (GRCm39)	missense	probably benign
R0741:Or10a3m	UTSW	7	108,312,811 (GRCm39)	missense	probably benign	0.00
R1515:Or10a3m	UTSW	7	108,313,148 (GRCm39)	missense	possibly damaging	0.94
R1982:Or10a3m	UTSW	7	108,312,902 (GRCm39)	missense	probably damaging	1.00
R2176:Or10a3m	UTSW	7	108,313,339 (GRCm39)	missense	probably damaging	1.00
R3967:Or10a3m	UTSW	7	108,313,060 (GRCm39)	missense	probably benign
R4009:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R4010:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R4011:Or10a3m	UTSW	7	108,313,366 (GRCm39)	missense	probably benign	0.00
R5095:Or10a3m	UTSW	7	108,313,019 (GRCm39)	missense	probably damaging	1.00
R5271:Or10a3m	UTSW	7	108,313,424 (GRCm39)	missense	probably damaging	1.00
R5926:Or10a3m	UTSW	7	108,312,794 (GRCm39)	missense	probably damaging	1.00
R6295:Or10a3m	UTSW	7	108,312,845 (GRCm39)	missense	probably damaging	0.98
R6528:Or10a3m	UTSW	7	108,312,638 (GRCm39)	missense	probably damaging	1.00
R6624:Or10a3m	UTSW	7	108,312,743 (GRCm39)	missense	possibly damaging	0.50
R8029:Or10a3m	UTSW	7	108,313,037 (GRCm39)	missense	possibly damaging	0.70
R8443:Or10a3m	UTSW	7	108,313,418 (GRCm39)	missense	possibly damaging	0.79
R8737:Or10a3m	UTSW	7	108,312,964 (GRCm39)	missense	probably damaging	1.00
R9415:Or10a3m	UTSW	7	108,313,042 (GRCm39)	missense	probably damaging	1.00
R9622:Or10a3m	UTSW	7	108,312,677 (GRCm39)	missense	probably benign	0.30
X0023:Or10a3m	UTSW	7	108,313,217 (GRCm39)	missense	possibly damaging	0.79
Z1088:Or10a3m	UTSW	7	108,312,745 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- CAAAACTGCTAATCTGAACCTCTG -3'
(R):5'- TCAGGACCACAAGCATTTTAGGTG -3'

Sequencing Primer
(F):5'- GAACCTCTGTTAAAAAGAGCATGTC -3'
(R):5'- TAGGTGTGATAACTGTGCTGAAAC -3'

Posted On

2017-02-10