Mutagenetix > Incidental Mutation

Incidental Mutation 'R5881:Ros1'

454428

Institutional Source

Beutler Lab

Gene Symbol

Ros1

Ensembl Gene

ENSMUSG00000019893

Gene Name

Ros1 proto-oncogene

Synonyms

Ros-1, c-ros

MMRRC Submission

044085-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.142) question?

Stock #

R5881 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

51921817-52071340 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 52057894 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 51 (R51Q)

Ref Sequence

ENSEMBL: ENSMUSP00000151720 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020045] [ENSMUST00000218452] [ENSMUST00000219173] [ENSMUST00000219692]

AlphaFold

Q78DX7

Predicted Effect

probably benign
Transcript: ENSMUST00000020045
AA Change: R51Q

PolyPhen 2 Score 0.172 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000020045
Gene: ENSMUSG00000019893
AA Change: R51Q

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	568	654	2.24e-4	SMART
LY	734	776	2.28e1	SMART
LY	777	815	4.61e0	SMART
FN3	944	1023	5.53e-4	SMART
FN3	1037	1133	1.07e1	SMART
FN3	1440	1532	1.19e1	SMART
FN3	1551	1637	2.11e0	SMART
FN3	1649	1731	6.8e-4	SMART
FN3	1746	1832	2.7e1	SMART
TyrKc	1938	2208	1.3e-145	SMART
low complexity region	2294	2307	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000117992
AA Change: R51Q

PolyPhen 2 Score 0.172 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000112873
Gene: ENSMUSG00000019893
AA Change: R51Q

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	547	633	2.24e-4	SMART
LY	713	755	2.28e1	SMART
LY	756	794	4.61e0	SMART
FN3	923	1002	5.53e-4	SMART
FN3	1016	1112	1.07e1	SMART
FN3	1419	1511	1.19e1	SMART
FN3	1530	1616	2.11e0	SMART
FN3	1628	1710	6.8e-4	SMART
FN3	1725	1811	2.7e1	SMART
TyrKc	1917	2187	1.3e-145	SMART
low complexity region	2273	2286	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000175892
AA Change: R51Q

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000135235
Gene: ENSMUSG00000019893
AA Change: R51Q

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	100	178	1.05e-4	SMART
FN3	196	273	7.45e-10	SMART
Blast:LY	360	400	1e-20	BLAST
Blast:LY	449	486	4e-15	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000177378
AA Change: R51Q

PolyPhen 2 Score 0.082 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000134905
Gene: ENSMUSG00000019893
AA Change: R51Q

Domain	Start	End	E-Value	Type
transmembrane domain	7	26	N/A	INTRINSIC
FN3	109	187	1.05e-4	SMART
FN3	205	282	7.45e-10	SMART
LY	369	409	9.17e0	SMART
FN3	547	633	2.24e-4	SMART
LY	713	755	2.28e1	SMART
LY	756	794	4.61e0	SMART
FN3	923	1002	5.53e-4	SMART
FN3	1016	1112	1.07e1	SMART
Blast:LY	1190	1236	2e-18	BLAST
FN3	1419	1511	1.19e1	SMART
FN3	1530	1616	2.11e0	SMART
FN3	1628	1710	6.8e-4	SMART
FN3	1725	1811	2.7e1	SMART
transmembrane domain	1832	1854	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000218452
AA Change: R51Q

PolyPhen 2 Score 0.264 (Sensitivity: 0.91; Specificity: 0.88)

Predicted Effect

probably benign
Transcript: ENSMUST00000219173
AA Change: R51Q

PolyPhen 2 Score 0.133 (Sensitivity: 0.92; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000219692
AA Change: R51Q

PolyPhen 2 Score 0.230 (Sensitivity: 0.91; Specificity: 0.88)

Coding Region Coverage

1x: 99.9%
3x: 99.3%
10x: 94.5%
20x: 78.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations exhibit male infertility due to impaired sperm maturation in the epididymis. Mutant sperm are capable of fertilization in vitro but not in vivo. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abi3	A	C	11: 95,725,213 (GRCm39)	L159W	probably damaging	Het
Abi3	A	T	11: 95,725,214 (GRCm39)	L159M	probably damaging	Het
Adam11	A	C	11: 102,664,636 (GRCm39)	I406L	probably benign	Het
Ager	G	T	17: 34,819,051 (GRCm39)	V300L	probably damaging	Het
Ank3	C	T	10: 69,822,660 (GRCm39)	S1726L	probably benign	Het
Boc	A	G	16: 44,311,014 (GRCm39)	I740T	probably damaging	Het
C8a	T	A	4: 104,711,129 (GRCm39)	D178V	probably damaging	Het
Ces3a	G	A	8: 105,777,198 (GRCm39)	V174M	probably damaging	Het
Cisd1	A	T	10: 71,172,244 (GRCm39)	W13R	probably damaging	Het
Commd8	A	T	5: 72,320,107 (GRCm39)	D111E	probably benign	Het
Ddx60	A	T	8: 62,490,104 (GRCm39)	D1691V	probably damaging	Het
Desi2	A	G	1: 178,065,479 (GRCm39)	Y15C	probably damaging	Het
Dnmt1	A	G	9: 20,864,013 (GRCm39)	V24A	probably damaging	Het
Dock10	G	A	1: 80,538,640 (GRCm39)	T968M	probably benign	Het
Dsel	A	T	1: 111,787,168 (GRCm39)	F1122L	probably damaging	Het
Eml3	T	C	19: 8,910,807 (GRCm39)	F220L	probably damaging	Het
Entpd2	A	C	2: 25,290,824 (GRCm39)	N443H	probably damaging	Het
Epgn	A	G	5: 91,176,222 (GRCm39)	N36S	probably benign	Het
Fsip2	G	T	2: 82,814,785 (GRCm39)	S3506I	possibly damaging	Het
Gprin3	T	C	6: 59,331,771 (GRCm39)	S179G	probably benign	Het
Grifin	G	T	5: 140,549,342 (GRCm39)	H125N	possibly damaging	Het
H1f9	A	G	11: 94,858,989 (GRCm39)	T95A	possibly damaging	Het
Hmcn1	A	G	1: 150,506,078 (GRCm39)	V3816A	probably damaging	Het
Hspa14	A	G	2: 3,499,207 (GRCm39)	F196L	probably benign	Het
Ice1	T	C	13: 70,754,620 (GRCm39)	K489E	probably benign	Het
Jmjd6	C	T	11: 116,730,682 (GRCm39)	W117*	probably null	Het
Lrit2	G	T	14: 36,794,192 (GRCm39)	A419S	probably benign	Het
Mc3r	T	C	2: 172,091,092 (GRCm39)	S105P	probably benign	Het
Mccc1	A	G	3: 36,018,531 (GRCm39)	V601A	probably benign	Het
Myo1f	A	G	17: 33,795,627 (GRCm39)	D91G	probably damaging	Het
Myo1f	G	A	17: 33,799,259 (GRCm39)	E255K	possibly damaging	Het
Necap1	T	A	6: 122,858,503 (GRCm39)	D115E	probably benign	Het
Or4b12	A	T	2: 90,096,786 (GRCm39)		probably null	Het
Or4k15b	G	A	14: 50,272,444 (GRCm39)	R139C	probably benign	Het
Or51b6	T	A	7: 103,555,883 (GRCm39)	M79K	probably damaging	Het
Papln	A	G	12: 83,818,652 (GRCm39)	E78G	probably null	Het
Phldb3	T	C	7: 24,326,147 (GRCm39)		probably null	Het
Pkd1l2	A	G	8: 117,724,321 (GRCm39)	I2394T	probably damaging	Het
Ppa2	A	T	3: 133,036,200 (GRCm39)	N118I	probably damaging	Het
Ppef2	A	T	5: 92,398,388 (GRCm39)	C43*	probably null	Het
Pramel51	A	G	12: 88,143,111 (GRCm39)	L169P	probably damaging	Het
Rab27a	G	A	9: 72,992,321 (GRCm39)		probably null	Het
Rabgap1l	A	T	1: 160,169,683 (GRCm39)	F47I	probably damaging	Het
Rala	A	G	13: 18,067,746 (GRCm39)	I95T	probably damaging	Het
Rgl2	A	G	17: 34,151,691 (GRCm39)	D245G	probably benign	Het
Rnf34	A	T	5: 123,002,146 (GRCm39)	T35S	probably damaging	Het
Samd9l	T	C	6: 3,372,716 (GRCm39)	D1515G	possibly damaging	Het
Scn7a	T	C	2: 66,505,870 (GRCm39)	E1673G	probably benign	Het
Slc6a20a	A	C	9: 123,470,773 (GRCm39)		probably null	Het
Tdrd5	A	T	1: 156,122,070 (GRCm39)	S280T	probably damaging	Het
Tent2	A	T	13: 93,312,246 (GRCm39)	C180*	probably null	Het
Tie1	T	C	4: 118,332,800 (GRCm39)	I911V	possibly damaging	Het
Vipas39	G	A	12: 87,298,581 (GRCm39)	R194*	probably null	Het
Ybx3	A	C	6: 131,345,451 (GRCm39)	N307K	possibly damaging	Het
Ylpm1	A	T	12: 85,088,899 (GRCm39)	H1216L	probably damaging	Het

Other mutations in Ros1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Ros1	APN	10	52,070,986 (GRCm39)	missense	probably benign	0.01
IGL00338:Ros1	APN	10	52,001,907 (GRCm39)	missense	probably benign
IGL00419:Ros1	APN	10	51,967,150 (GRCm39)	missense	probably damaging	0.97
IGL00840:Ros1	APN	10	52,020,969 (GRCm39)	missense	possibly damaging	0.92
IGL00841:Ros1	APN	10	52,020,969 (GRCm39)	missense	possibly damaging	0.92
IGL00951:Ros1	APN	10	52,019,348 (GRCm39)	missense	probably damaging	0.99
IGL01123:Ros1	APN	10	51,996,905 (GRCm39)	missense	probably damaging	1.00
IGL01128:Ros1	APN	10	52,018,424 (GRCm39)	nonsense	probably null
IGL01300:Ros1	APN	10	51,977,809 (GRCm39)	missense	probably benign	0.01
IGL01316:Ros1	APN	10	51,963,975 (GRCm39)	critical splice donor site	probably null
IGL01349:Ros1	APN	10	51,927,122 (GRCm39)	missense	probably damaging	0.99
IGL01363:Ros1	APN	10	52,042,238 (GRCm39)	missense	probably damaging	1.00
IGL01457:Ros1	APN	10	51,922,426 (GRCm39)	splice site	probably benign
IGL01532:Ros1	APN	10	51,967,034 (GRCm39)	splice site	probably benign
IGL01585:Ros1	APN	10	52,031,198 (GRCm39)	missense	probably damaging	1.00
IGL01650:Ros1	APN	10	52,031,075 (GRCm39)	missense	probably damaging	0.99
IGL01672:Ros1	APN	10	51,977,899 (GRCm39)	missense	possibly damaging	0.92
IGL01904:Ros1	APN	10	51,954,007 (GRCm39)	missense	probably damaging	0.97
IGL02040:Ros1	APN	10	51,992,018 (GRCm39)	missense	probably damaging	0.99
IGL02053:Ros1	APN	10	52,038,816 (GRCm39)	missense	probably damaging	1.00
IGL02147:Ros1	APN	10	51,996,991 (GRCm39)	missense	probably damaging	1.00
IGL02169:Ros1	APN	10	51,958,053 (GRCm39)	critical splice donor site	probably null
IGL02247:Ros1	APN	10	52,005,677 (GRCm39)	missense	probably damaging	0.99
IGL02262:Ros1	APN	10	52,055,065 (GRCm39)	missense	probably damaging	0.96
IGL02307:Ros1	APN	10	52,004,534 (GRCm39)	missense	possibly damaging	0.53
IGL02398:Ros1	APN	10	52,020,980 (GRCm39)	splice site	probably benign
IGL02525:Ros1	APN	10	51,992,138 (GRCm39)	missense	possibly damaging	0.66
IGL02718:Ros1	APN	10	51,994,328 (GRCm39)	missense	probably damaging	1.00
IGL02721:Ros1	APN	10	52,048,927 (GRCm39)	splice site	probably benign
IGL02808:Ros1	APN	10	52,001,985 (GRCm39)	missense	probably damaging	1.00
IGL03009:Ros1	APN	10	52,022,003 (GRCm39)	missense	probably benign	0.00
IGL03035:Ros1	APN	10	51,952,080 (GRCm39)	splice site	probably benign
IGL03092:Ros1	APN	10	51,974,902 (GRCm39)	missense	probably damaging	0.99
IGL03309:Ros1	APN	10	51,994,357 (GRCm39)	missense	possibly damaging	0.83
IGL03333:Ros1	APN	10	52,031,267 (GRCm39)	missense	probably damaging	1.00
boss	UTSW	10	51,967,091 (GRCm39)	nonsense	probably null
Chuckwagon	UTSW	10	51,994,299 (GRCm39)	missense	probably damaging	1.00
R1005_Ros1_648	UTSW	10	52,004,501 (GRCm39)	splice site	probably benign
R1220_Ros1_012	UTSW	10	51,974,966 (GRCm39)	missense	probably damaging	0.97
R3423_Ros1_122	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
trail	UTSW	10	52,037,991 (GRCm39)	nonsense	probably null
R0049:Ros1	UTSW	10	51,977,857 (GRCm39)	missense	possibly damaging	0.66
R0049:Ros1	UTSW	10	51,977,857 (GRCm39)	missense	possibly damaging	0.66
R0050:Ros1	UTSW	10	51,977,899 (GRCm39)	missense	probably damaging	0.97
R0050:Ros1	UTSW	10	51,977,899 (GRCm39)	missense	probably damaging	0.97
R0057:Ros1	UTSW	10	52,056,287 (GRCm39)	missense	probably benign	0.00
R0057:Ros1	UTSW	10	52,056,287 (GRCm39)	missense	probably benign	0.00
R0106:Ros1	UTSW	10	52,018,363 (GRCm39)	missense	possibly damaging	0.85
R0106:Ros1	UTSW	10	52,018,363 (GRCm39)	missense	possibly damaging	0.85
R0125:Ros1	UTSW	10	52,001,885 (GRCm39)	missense	probably benign	0.38
R0403:Ros1	UTSW	10	52,019,534 (GRCm39)	splice site	probably benign
R0487:Ros1	UTSW	10	52,031,204 (GRCm39)	missense	possibly damaging	0.69
R0502:Ros1	UTSW	10	52,070,919 (GRCm39)	splice site	probably benign
R0557:Ros1	UTSW	10	51,961,359 (GRCm39)	missense	possibly damaging	0.82
R0599:Ros1	UTSW	10	51,999,396 (GRCm39)	missense	probably damaging	1.00
R0620:Ros1	UTSW	10	51,994,444 (GRCm39)	missense	probably damaging	1.00
R0679:Ros1	UTSW	10	51,942,391 (GRCm39)	missense	possibly damaging	0.95
R1005:Ros1	UTSW	10	52,004,501 (GRCm39)	splice site	probably benign
R1073:Ros1	UTSW	10	51,922,221 (GRCm39)	missense	probably damaging	1.00
R1220:Ros1	UTSW	10	51,974,966 (GRCm39)	missense	probably damaging	0.97
R1279:Ros1	UTSW	10	52,018,262 (GRCm39)	missense	possibly damaging	0.81
R1295:Ros1	UTSW	10	51,964,028 (GRCm39)	missense	possibly damaging	0.92
R1336:Ros1	UTSW	10	52,044,758 (GRCm39)	missense	probably damaging	1.00
R1371:Ros1	UTSW	10	51,964,041 (GRCm39)	missense	probably damaging	0.98
R1447:Ros1	UTSW	10	51,974,954 (GRCm39)	missense	possibly damaging	0.66
R1486:Ros1	UTSW	10	52,048,954 (GRCm39)	missense	probably damaging	1.00
R1499:Ros1	UTSW	10	51,974,773 (GRCm39)	missense	possibly damaging	0.92
R1669:Ros1	UTSW	10	52,037,907 (GRCm39)	missense	probably damaging	1.00
R1744:Ros1	UTSW	10	51,999,475 (GRCm39)	missense	probably damaging	0.99
R1759:Ros1	UTSW	10	51,996,922 (GRCm39)	missense	probably damaging	1.00
R1791:Ros1	UTSW	10	51,976,183 (GRCm39)	missense	probably benign	0.00
R1794:Ros1	UTSW	10	52,000,199 (GRCm39)	nonsense	probably null
R2031:Ros1	UTSW	10	51,943,164 (GRCm39)	missense	possibly damaging	0.88
R2115:Ros1	UTSW	10	52,004,651 (GRCm39)	missense	probably benign	0.00
R2219:Ros1	UTSW	10	52,042,175 (GRCm39)	missense	probably damaging	1.00
R2290:Ros1	UTSW	10	51,994,477 (GRCm39)	missense	probably damaging	0.96
R2329:Ros1	UTSW	10	52,038,983 (GRCm39)	missense	probably damaging	1.00
R2371:Ros1	UTSW	10	52,039,991 (GRCm39)	missense	possibly damaging	0.66
R2879:Ros1	UTSW	10	52,048,936 (GRCm39)	critical splice donor site	probably null
R3154:Ros1	UTSW	10	51,927,077 (GRCm39)	missense	probably benign
R3423:Ros1	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
R3424:Ros1	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
R3425:Ros1	UTSW	10	52,004,512 (GRCm39)	splice site	probably null
R3433:Ros1	UTSW	10	51,967,204 (GRCm39)	missense	probably benign	0.45
R3522:Ros1	UTSW	10	51,967,091 (GRCm39)	nonsense	probably null
R3686:Ros1	UTSW	10	52,021,912 (GRCm39)	missense	probably damaging	1.00
R3710:Ros1	UTSW	10	52,037,991 (GRCm39)	nonsense	probably null
R3771:Ros1	UTSW	10	52,005,087 (GRCm39)	missense	probably damaging	0.97
R3808:Ros1	UTSW	10	51,996,944 (GRCm39)	missense	probably benign	0.08
R3930:Ros1	UTSW	10	52,070,944 (GRCm39)	missense	possibly damaging	0.92
R3950:Ros1	UTSW	10	51,942,484 (GRCm39)	missense	probably damaging	1.00
R3981:Ros1	UTSW	10	51,996,974 (GRCm39)	missense	possibly damaging	0.46
R4007:Ros1	UTSW	10	51,994,328 (GRCm39)	missense	probably damaging	1.00
R4346:Ros1	UTSW	10	52,044,705 (GRCm39)	missense	possibly damaging	0.92
R4382:Ros1	UTSW	10	51,997,055 (GRCm39)	missense	possibly damaging	0.46
R4414:Ros1	UTSW	10	52,038,800 (GRCm39)	critical splice donor site	probably null
R4450:Ros1	UTSW	10	51,954,038 (GRCm39)	missense	probably damaging	0.98
R4468:Ros1	UTSW	10	51,994,452 (GRCm39)	missense	probably damaging	1.00
R4569:Ros1	UTSW	10	52,040,090 (GRCm39)	missense	probably damaging	0.99
R4649:Ros1	UTSW	10	52,005,764 (GRCm39)	missense	possibly damaging	0.66
R4684:Ros1	UTSW	10	52,005,192 (GRCm39)	missense	probably damaging	1.00
R4706:Ros1	UTSW	10	51,977,990 (GRCm39)	missense	possibly damaging	0.95
R4731:Ros1	UTSW	10	52,018,325 (GRCm39)	missense	probably damaging	1.00
R4748:Ros1	UTSW	10	51,992,093 (GRCm39)	missense	probably benign	0.00
R4806:Ros1	UTSW	10	51,972,271 (GRCm39)	missense	probably damaging	0.96
R4865:Ros1	UTSW	10	52,048,966 (GRCm39)	missense	probably damaging	0.99
R4973:Ros1	UTSW	10	52,031,087 (GRCm39)	missense	probably damaging	0.98
R5022:Ros1	UTSW	10	52,000,171 (GRCm39)	missense	possibly damaging	0.46
R5033:Ros1	UTSW	10	52,004,512 (GRCm39)	critical splice donor site	probably null
R5082:Ros1	UTSW	10	52,040,037 (GRCm39)	missense	possibly damaging	0.66
R5083:Ros1	UTSW	10	52,040,037 (GRCm39)	missense	possibly damaging	0.66
R5130:Ros1	UTSW	10	52,040,037 (GRCm39)	missense	possibly damaging	0.66
R5269:Ros1	UTSW	10	51,927,104 (GRCm39)	missense	probably damaging	1.00
R5399:Ros1	UTSW	10	51,967,040 (GRCm39)	critical splice donor site	probably null
R5414:Ros1	UTSW	10	52,031,189 (GRCm39)	missense	probably damaging	1.00
R5659:Ros1	UTSW	10	52,019,482 (GRCm39)	missense	possibly damaging	0.92
R5742:Ros1	UTSW	10	52,018,234 (GRCm39)	critical splice donor site	probably null
R5780:Ros1	UTSW	10	52,070,953 (GRCm39)	missense	probably damaging	1.00
R5805:Ros1	UTSW	10	51,999,385 (GRCm39)	missense	probably damaging	1.00
R5843:Ros1	UTSW	10	52,042,293 (GRCm39)	missense	possibly damaging	0.92
R6027:Ros1	UTSW	10	52,040,064 (GRCm39)	missense	possibly damaging	0.82
R6035:Ros1	UTSW	10	51,954,067 (GRCm39)	missense	probably benign
R6035:Ros1	UTSW	10	51,954,067 (GRCm39)	missense	probably benign
R6052:Ros1	UTSW	10	52,039,999 (GRCm39)	missense	probably benign	0.39
R6175:Ros1	UTSW	10	51,977,881 (GRCm39)	missense	probably benign	0.02
R6315:Ros1	UTSW	10	51,994,306 (GRCm39)	missense	probably benign
R6342:Ros1	UTSW	10	52,031,351 (GRCm39)	missense	probably damaging	1.00
R6470:Ros1	UTSW	10	52,042,140 (GRCm39)	critical splice donor site	probably null
R6527:Ros1	UTSW	10	52,019,473 (GRCm39)	missense	possibly damaging	0.66
R6568:Ros1	UTSW	10	52,038,908 (GRCm39)	missense	probably damaging	1.00
R6573:Ros1	UTSW	10	52,031,106 (GRCm39)	missense	possibly damaging	0.84
R6653:Ros1	UTSW	10	52,018,299 (GRCm39)	missense	probably damaging	1.00
R6959:Ros1	UTSW	10	52,040,090 (GRCm39)	missense	probably damaging	0.99
R7011:Ros1	UTSW	10	52,056,272 (GRCm39)	missense	probably damaging	1.00
R7111:Ros1	UTSW	10	52,057,906 (GRCm39)	missense	probably benign	0.02
R7243:Ros1	UTSW	10	51,999,477 (GRCm39)	missense	probably damaging	1.00
R7355:Ros1	UTSW	10	52,042,175 (GRCm39)	missense	probably damaging	1.00
R7385:Ros1	UTSW	10	52,031,222 (GRCm39)	missense	probably benign	0.00
R7460:Ros1	UTSW	10	51,994,299 (GRCm39)	missense	probably damaging	1.00
R7549:Ros1	UTSW	10	52,021,930 (GRCm39)	missense	probably damaging	0.96
R7573:Ros1	UTSW	10	52,046,072 (GRCm39)	missense	probably benign	0.03
R7650:Ros1	UTSW	10	51,922,305 (GRCm39)	missense	probably benign	0.00
R7667:Ros1	UTSW	10	52,040,067 (GRCm39)	missense	probably damaging	1.00
R7696:Ros1	UTSW	10	52,018,379 (GRCm39)	missense	probably damaging	1.00
R7785:Ros1	UTSW	10	52,038,944 (GRCm39)	missense	probably damaging	1.00
R7814:Ros1	UTSW	10	51,972,233 (GRCm39)	missense	probably benign	0.28
R7830:Ros1	UTSW	10	52,031,030 (GRCm39)	missense	probably damaging	0.99
R7832:Ros1	UTSW	10	52,020,957 (GRCm39)	missense	probably damaging	0.99
R7854:Ros1	UTSW	10	52,004,563 (GRCm39)	missense	probably damaging	1.00
R7912:Ros1	UTSW	10	52,044,791 (GRCm39)	missense	probably damaging	1.00
R7972:Ros1	UTSW	10	52,030,926 (GRCm39)	nonsense	probably null
R7993:Ros1	UTSW	10	51,999,443 (GRCm39)	missense	probably benign	0.34
R8036:Ros1	UTSW	10	52,041,439 (GRCm39)	missense	probably benign
R8137:Ros1	UTSW	10	52,001,933 (GRCm39)	missense	possibly damaging	0.87
R8169:Ros1	UTSW	10	51,940,768 (GRCm39)	critical splice donor site	probably null
R8199:Ros1	UTSW	10	51,977,813 (GRCm39)	nonsense	probably null
R8293:Ros1	UTSW	10	51,964,014 (GRCm39)	missense	probably damaging	1.00
R8368:Ros1	UTSW	10	51,940,833 (GRCm39)	missense	probably damaging	1.00
R8406:Ros1	UTSW	10	51,977,941 (GRCm39)	missense	possibly damaging	0.56
R8471:Ros1	UTSW	10	51,997,078 (GRCm39)	missense	probably benign	0.00
R8498:Ros1	UTSW	10	52,055,047 (GRCm39)	missense	probably damaging	0.99
R8532:Ros1	UTSW	10	51,974,852 (GRCm39)	missense	possibly damaging	0.92
R8678:Ros1	UTSW	10	51,963,998 (GRCm39)	missense	probably benign
R8726:Ros1	UTSW	10	51,954,769 (GRCm39)	missense	possibly damaging	0.46
R8789:Ros1	UTSW	10	51,999,328 (GRCm39)	missense	probably damaging	0.99
R8799:Ros1	UTSW	10	51,922,143 (GRCm39)	missense	probably benign	0.08
R8915:Ros1	UTSW	10	51,977,805 (GRCm39)	splice site	probably benign
R8958:Ros1	UTSW	10	51,972,190 (GRCm39)	missense	probably damaging	1.00
R8972:Ros1	UTSW	10	51,999,333 (GRCm39)	missense	probably benign	0.05
R9020:Ros1	UTSW	10	52,031,023 (GRCm39)	missense	probably benign	0.32
R9147:Ros1	UTSW	10	51,927,039 (GRCm39)	missense	probably benign
R9154:Ros1	UTSW	10	51,922,301 (GRCm39)	missense	possibly damaging	0.87
R9189:Ros1	UTSW	10	52,019,502 (GRCm39)	missense	probably damaging	0.99
R9341:Ros1	UTSW	10	51,972,116 (GRCm39)	critical splice donor site	probably null
R9343:Ros1	UTSW	10	51,972,116 (GRCm39)	critical splice donor site	probably null
R9407:Ros1	UTSW	10	51,994,491 (GRCm39)	missense	probably damaging	1.00
R9428:Ros1	UTSW	10	51,958,061 (GRCm39)	missense	probably benign	0.00
R9502:Ros1	UTSW	10	52,000,174 (GRCm39)	missense	probably benign	0.00
R9531:Ros1	UTSW	10	52,007,063 (GRCm39)	missense	probably damaging	1.00
R9546:Ros1	UTSW	10	51,994,215 (GRCm39)	critical splice donor site	probably null
R9562:Ros1	UTSW	10	51,943,170 (GRCm39)	missense	probably damaging	1.00
R9565:Ros1	UTSW	10	51,943,170 (GRCm39)	missense	probably damaging	1.00
R9604:Ros1	UTSW	10	51,994,249 (GRCm39)	missense	probably damaging	1.00
R9645:Ros1	UTSW	10	51,948,148 (GRCm39)	critical splice donor site	probably null
R9658:Ros1	UTSW	10	51,967,069 (GRCm39)	missense	probably damaging	0.99
R9664:Ros1	UTSW	10	51,996,931 (GRCm39)	missense	probably benign	0.18
RF018:Ros1	UTSW	10	52,031,217 (GRCm39)	missense	probably benign
Z1176:Ros1	UTSW	10	51,967,205 (GRCm39)	missense	possibly damaging	0.89
Z1177:Ros1	UTSW	10	52,044,767 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCATGAATCTGTGTTAGTCCTTTGC -3'
(R):5'- TGGCAAATCACTCTACTACTGGG -3'

Sequencing Primer
(F):5'- AATTCTATAATTCAGGCTTCTCACTG -3'
(R):5'- TCACTCTACTACTGGGAAACAAATG -3'

Posted On

2017-02-10