Mutagenetix > Incidental Mutation

Incidental Mutation 'R4908:Lasp1'

455816

Institutional Source

Beutler Lab

Gene Symbol

Lasp1

Ensembl Gene

ENSMUSG00000038366

Gene Name

LIM and SH3 protein 1

Synonyms

SH3P6, Def-4

MMRRC Submission

042510-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.235) question?

Stock #

R4908 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

97689826-97729590 bp(+) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

T to A at 97724530 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000123050 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043843] [ENSMUST00000129558] [ENSMUST00000129828] [ENSMUST00000146572] [ENSMUST00000148280] [ENSMUST00000152962]

AlphaFold

Q61792

Predicted Effect

probably null
Transcript: ENSMUST00000043843

SMART Domains

Protein: ENSMUSP00000042123
Gene: ENSMUSG00000038366

Domain	Start	End	E-Value	Type
LIM	4	56	4.34e-15	SMART
NEBU	62	92	1.1e-8	SMART
NEBU	98	128	1.05e-9	SMART
low complexity region	174	180	N/A	INTRINSIC
SH3	207	263	8.11e-17	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000107568

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127575

Predicted Effect

probably benign
Transcript: ENSMUST00000129558

SMART Domains

Protein: ENSMUSP00000123165
Gene: ENSMUSG00000038366

Domain	Start	End	E-Value	Type
Blast:LIM	1	20	4e-7	BLAST
NEBU	26	56	1.1e-8	SMART
NEBU	62	92	1.05e-9	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000129828

SMART Domains

Protein: ENSMUSP00000115308
Gene: ENSMUSG00000038366

Domain	Start	End	E-Value	Type
Blast:LIM	1	20	4e-7	BLAST
NEBU	26	56	1.1e-8	SMART
NEBU	62	92	1.05e-9	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000146572

SMART Domains

Protein: ENSMUSP00000121907
Gene: ENSMUSG00000038366

Domain	Start	End	E-Value	Type
Blast:LIM	1	20	3e-7	BLAST
Pfam:Nebulin	31	59	2.1e-12	PFAM
Pfam:Nebulin	67	89	1.5e-9	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000148280

SMART Domains

Protein: ENSMUSP00000123050
Gene: ENSMUSG00000038366

Domain	Start	End	E-Value	Type
Blast:LIM	1	20	2e-7	BLAST
NEBU	26	56	1.1e-8	SMART
NEBU	62	92	1.05e-9	SMART
low complexity region	138	144	N/A	INTRINSIC
SCOP:d1awj__	155	186	7e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000152962

SMART Domains

Protein: ENSMUSP00000120645
Gene: ENSMUSG00000038366

Domain	Start	End	E-Value	Type
Blast:LIM	1	20	4e-7	BLAST
NEBU	26	56	1.1e-8	SMART
NEBU	62	92	1.05e-9	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.2%
20x: 92.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3, and also a member of the nebulin family of actin-binding proteins. The encoded protein is a cAMP and cGMP dependent signaling protein and binds to the actin cytoskeleton at extensions of the cell membrane. The encoded protein has been linked to metastatic breast cancer, hematopoetic tumors such as B-cell lymphomas, and colorectal cancer. [provided by RefSeq, Oct 2012]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced histamine-stimulated gastric acid secretion and enlarged heart and testes on a mixed background. Mice homozygous for a transgene insertion exhibit abnormal tail vertebrae with scoliosis, transient spina bifida occulta, and a bent tail. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 99 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110059G10Rik	T	A	9: 122,778,008 (GRCm39)	I79L	probably benign	Het
Ablim2	C	T	5: 35,959,766 (GRCm39)	R73C	possibly damaging	Het
Acaa1a	G	A	9: 119,177,772 (GRCm39)	S218N	probably benign	Het
Acsm5	A	T	7: 119,137,314 (GRCm39)	I377F	probably damaging	Het
Ahnak2	T	C	12: 112,741,706 (GRCm39)	T789A	probably benign	Het
Ak9	A	T	10: 41,296,678 (GRCm39)	T1475S	unknown	Het
AU040320	A	T	4: 126,747,081 (GRCm39)	N1028Y	probably damaging	Het
Bahcc1	T	A	11: 120,178,580 (GRCm39)	S2380T	probably benign	Het
Cadps	A	G	14: 12,536,386 (GRCm38)	Y525H	probably damaging	Het
Casp8ap2	A	G	4: 32,639,905 (GRCm39)	T320A	possibly damaging	Het
Ccdc138	A	G	10: 58,380,817 (GRCm39)	N483D	possibly damaging	Het
Ccdc39	G	A	3: 33,893,242 (GRCm39)		probably null	Het
Cd300c2	T	C	11: 114,887,772 (GRCm39)	N210S	probably damaging	Het
Cd84	A	G	1: 171,700,432 (GRCm39)	D183G	probably damaging	Het
Cep95	C	T	11: 106,702,172 (GRCm39)	P390S	probably damaging	Het
Chd9	A	T	8: 91,741,877 (GRCm39)	H1622L	possibly damaging	Het
Cilp	C	A	9: 65,185,302 (GRCm39)	Q466K	probably benign	Het
Cinp	T	A	12: 110,850,487 (GRCm39)	T5S	probably damaging	Het
Clec4a2	T	C	6: 123,119,462 (GRCm39)	L238S	probably damaging	Het
Cntrob	T	C	11: 69,211,732 (GRCm39)	Y164C	probably damaging	Het
Col6a3	A	G	1: 90,735,246 (GRCm39)	L1408P	probably damaging	Het
Cul3	G	A	1: 80,258,632 (GRCm39)	S468L	possibly damaging	Het
Dnah11	T	A	12: 118,090,618 (GRCm39)	D1081V	probably benign	Het
Dnah2	C	A	11: 69,411,973 (GRCm39)	V263L	probably benign	Het
Efna3	C	G	3: 89,222,805 (GRCm39)	R185P	probably damaging	Het
F5	A	T	1: 164,039,389 (GRCm39)	I2000F	probably damaging	Het
Fancm	G	A	12: 65,141,645 (GRCm39)	G422E	probably benign	Het
Gcnt2	A	T	13: 41,014,210 (GRCm39)	D127V	probably damaging	Het
Gm21798	G	T	15: 64,689,618 (GRCm39)		probably benign	Het
Gramd1a	A	C	7: 30,838,292 (GRCm39)	S320R	probably benign	Het
Grn	T	C	11: 102,327,344 (GRCm39)		probably benign	Het
Helq	A	T	5: 100,910,507 (GRCm39)		probably null	Het
Herc2	A	G	7: 55,827,660 (GRCm39)	I2914V	probably benign	Het
Hnrnph1	T	C	11: 50,269,237 (GRCm39)	V27A	probably damaging	Het
Hs1bp3	G	T	12: 8,374,007 (GRCm39)	G182C	probably damaging	Het
Idnk	C	T	13: 58,311,267 (GRCm39)	P78L	probably benign	Het
Il10ra	T	C	9: 45,166,919 (GRCm39)	D544G	probably benign	Het
Inpp5e	T	C	2: 26,290,918 (GRCm39)	D383G	probably damaging	Het
Jak1	A	T	4: 101,036,911 (GRCm39)	V243D	probably damaging	Het
Kcnma1	A	T	14: 23,359,220 (GRCm39)	S1036T	probably damaging	Het
Kif26a	G	A	12: 112,123,776 (GRCm39)	C127Y	probably damaging	Het
Kif2c	T	C	4: 117,023,608 (GRCm39)	E368G	probably damaging	Het
Lcor	T	A	19: 41,572,601 (GRCm39)	V452D	probably benign	Het
Lrrtm1	C	G	6: 77,221,661 (GRCm39)	H373D	probably benign	Het
Matr3	T	A	18: 35,705,754 (GRCm39)	D226E	probably damaging	Het
Mmp17	G	A	5: 129,682,730 (GRCm39)	W456*	probably null	Het
Mpp4	T	C	1: 59,164,748 (GRCm39)	E463G	probably damaging	Het
Myh6	A	C	14: 55,194,419 (GRCm39)	F737V	probably damaging	Het
Nars2	T	A	7: 96,672,948 (GRCm39)	D271E	probably benign	Het
Nav2	A	G	7: 49,254,258 (GRCm39)	E2352G	probably damaging	Het
Nckap1	A	G	2: 80,353,718 (GRCm39)		probably null	Het
Nckap5	A	T	1: 125,955,324 (GRCm39)	S477R	probably damaging	Het
Nek11	T	G	9: 105,175,488 (GRCm39)	I319L	probably benign	Het
Neto2	A	G	8: 86,396,393 (GRCm39)	I84T	probably damaging	Het
Nlrp9a	A	T	7: 26,250,369 (GRCm39)	I45F	probably damaging	Het
Numa1	G	A	7: 101,662,012 (GRCm39)	R548H	probably damaging	Het
Or10a5	A	C	7: 106,635,364 (GRCm39)	M1L	probably benign	Het
Or14c39	A	C	7: 86,344,395 (GRCm39)	I244L	probably benign	Het
Or2ag1b	A	T	7: 106,288,740 (GRCm39)	L66H	probably damaging	Het
Or4c10	A	T	2: 89,760,923 (GRCm39)	M257L	probably benign	Het
Or4c100	T	A	2: 88,356,254 (GRCm39)	I109N	probably damaging	Het
Or4k36	T	A	2: 111,146,574 (GRCm39)	F250Y	probably benign	Het
Or7g25	A	G	9: 19,160,149 (GRCm39)	V182A	probably benign	Het
Or9m1	A	T	2: 87,733,533 (GRCm39)	N162K	probably damaging	Het
Pcdhb16	T	A	18: 37,612,894 (GRCm39)		probably null	Het
Pdcd10	T	C	3: 75,448,553 (GRCm39)	T4A	probably damaging	Het
Pgc	A	T	17: 48,039,819 (GRCm39)	Y71F	probably damaging	Het
Phlpp1	G	A	1: 106,317,481 (GRCm39)	G1234E	probably damaging	Het
Prr27	T	C	5: 87,990,888 (GRCm39)	F167L	probably benign	Het
Prrc2b	T	A	2: 32,116,330 (GRCm39)	S1421T	possibly damaging	Het
Pxmp2	A	G	5: 110,431,518 (GRCm39)	V75A	probably benign	Het
Pygl	A	T	12: 70,243,807 (GRCm39)	M545K	probably null	Het
Ranbp9	A	G	13: 43,574,733 (GRCm39)	Y412H	possibly damaging	Het
Rcc1	A	G	4: 132,065,064 (GRCm39)	V140A	probably damaging	Het
Reln	A	G	5: 22,184,718 (GRCm39)	V1599A	probably benign	Het
Rhebl1	A	T	15: 98,776,903 (GRCm39)	D122E	probably damaging	Het
Rock2	C	T	12: 17,009,492 (GRCm39)	L676F	probably benign	Het
Scn9a	A	T	2: 66,357,087 (GRCm39)	D1062E	probably benign	Het
Sec63	T	C	10: 42,681,186 (GRCm39)	I390T	probably damaging	Het
Slc10a6	T	C	5: 103,754,493 (GRCm39)	E346G	probably benign	Het
Slc12a3	G	A	8: 95,075,216 (GRCm39)	V737M	possibly damaging	Het
Slc12a8	T	C	16: 33,426,629 (GRCm39)		probably null	Het
Slc25a38	T	A	9: 119,949,354 (GRCm39)	I102N	probably damaging	Het
Spg7	T	A	8: 123,807,394 (GRCm39)	V390E	probably damaging	Het
Tbc1d20	T	C	2: 152,144,228 (GRCm39)	V75A	probably benign	Het
Tesk1	T	A	4: 43,445,555 (GRCm39)	C243*	probably null	Het
Ttk	T	A	9: 83,725,739 (GRCm39)	N220K	possibly damaging	Het
Ttll5	A	G	12: 85,965,948 (GRCm39)	E651G	probably benign	Het
Tubd1	T	C	11: 86,457,879 (GRCm39)	Y426H	probably damaging	Het
Uba6	T	C	5: 86,288,293 (GRCm39)		silent	Het
Ube2ql1	G	A	13: 69,852,289 (GRCm39)	R263W	probably damaging	Het
Vmn2r117	C	T	17: 23,678,812 (GRCm39)	G804D	probably damaging	Het
Vmn2r25	C	T	6: 123,805,406 (GRCm39)	E484K	probably benign	Het
Vmn2r4	T	A	3: 64,296,476 (GRCm39)	I770F	possibly damaging	Het
Vmn2r82	G	A	10: 79,214,589 (GRCm39)	V191M	probably benign	Het
Zbtb45	C	T	7: 12,742,037 (GRCm39)	V74M	probably damaging	Het
Zfp366	T	C	13: 99,370,609 (GRCm39)	V443A	possibly damaging	Het
Zfyve1	A	T	12: 83,598,345 (GRCm39)	C628S	probably damaging	Het
Zfyve26	A	T	12: 79,296,469 (GRCm39)		probably null	Het

Other mutations in Lasp1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01296:Lasp1	APN	11	97,727,016 (GRCm39)	missense	probably damaging	1.00
R0281:Lasp1	UTSW	11	97,697,677 (GRCm39)	nonsense	probably null
R2126:Lasp1	UTSW	11	97,726,960 (GRCm39)	missense	probably benign	0.34
R3906:Lasp1	UTSW	11	97,690,653 (GRCm39)	missense	probably damaging	1.00
R3908:Lasp1	UTSW	11	97,690,653 (GRCm39)	missense	probably damaging	1.00
R3909:Lasp1	UTSW	11	97,690,653 (GRCm39)	missense	probably damaging	1.00
R5239:Lasp1	UTSW	11	97,690,686 (GRCm39)	missense	probably damaging	1.00
R6519:Lasp1	UTSW	11	97,706,383 (GRCm39)	splice site	probably null
R6576:Lasp1	UTSW	11	97,724,402 (GRCm39)	missense	probably damaging	1.00
R6629:Lasp1	UTSW	11	97,697,722 (GRCm39)	nonsense	probably null
R7001:Lasp1	UTSW	11	97,697,659 (GRCm39)	missense	probably damaging	1.00
R8063:Lasp1	UTSW	11	97,724,957 (GRCm39)	missense	probably benign	0.00
R8708:Lasp1	UTSW	11	97,697,709 (GRCm39)	missense	possibly damaging	0.67
R9710:Lasp1	UTSW	11	97,697,593 (GRCm39)	start gained	probably benign

Predicted Primers

PCR Primer
(F):5'- AGAACTGGTTGGCTCGCAAC -3'
(R):5'- TTCATGCCAGGGCTGTATG -3'

Sequencing Primer
(F):5'- TTGGCTCGCAACGGAAC -3'
(R):5'- GGCTGTATGCTCCTCACACATAAATC -3'

Posted On

2017-02-14