Incidental Mutation 'R5887:Gpr155'
| ID |
456148 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr155
|
| Ensembl Gene |
ENSMUSG00000041762 |
| Gene Name |
G protein-coupled receptor 155 |
| Synonyms |
DEPDC3, 1110017O10Rik, PGR22, F730029F15Rik |
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R5887 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
73171850-73216842 bp(-) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
A to T
at 73174062 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Cysteine to Stop codon
at position 754
(C754*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000107675
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000076463]
[ENSMUST00000112043]
[ENSMUST00000112044]
|
| AlphaFold |
A2AWR3 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000076463
AA Change: C782*
|
| SMART Domains |
Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: C782*
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000112043
AA Change: C782*
|
| SMART Domains |
Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: C782*
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000112044
AA Change: C754*
|
| SMART Domains |
Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: C754*
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
Pfam:Mem_trans
|
187 |
360 |
8.3e-11 |
PFAM |
|
transmembrane domain
|
382 |
404 |
N/A |
INTRINSIC |
|
transmembrane domain
|
414 |
436 |
N/A |
INTRINSIC |
|
transmembrane domain
|
449 |
471 |
N/A |
INTRINSIC |
|
transmembrane domain
|
491 |
510 |
N/A |
INTRINSIC |
|
transmembrane domain
|
629 |
651 |
N/A |
INTRINSIC |
|
transmembrane domain
|
661 |
683 |
N/A |
INTRINSIC |
|
DEP
|
731 |
805 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000131430
|
| SMART Domains |
Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
98 |
120 |
N/A |
INTRINSIC |
|
transmembrane domain
|
130 |
152 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 99.8%
- 3x: 99.4%
- 10x: 97.2%
- 20x: 91.0%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 52 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930590J08Rik |
C |
T |
6: 91,892,124 (GRCm39) |
Q129* |
probably null |
Het |
| Acad8 |
A |
T |
9: 26,890,620 (GRCm39) |
|
probably null |
Het |
| AK157302 |
T |
C |
13: 21,679,579 (GRCm39) |
I35T |
possibly damaging |
Het |
| Arfgef3 |
G |
T |
10: 18,483,413 (GRCm39) |
S1437* |
probably null |
Het |
| Calcrl |
A |
T |
2: 84,200,841 (GRCm39) |
W68R |
probably damaging |
Het |
| Cd200l1 |
T |
A |
16: 45,238,279 (GRCm39) |
L178F |
probably damaging |
Het |
| Cd59a |
A |
G |
2: 103,934,546 (GRCm39) |
R5G |
probably damaging |
Het |
| Chl1 |
C |
T |
6: 103,694,565 (GRCm39) |
A1091V |
probably benign |
Het |
| Copg1 |
T |
C |
6: 87,879,279 (GRCm39) |
F442L |
probably damaging |
Het |
| Creld1 |
T |
C |
6: 113,469,860 (GRCm39) |
*421Q |
probably null |
Het |
| Crocc2 |
T |
A |
1: 93,121,838 (GRCm39) |
H662Q |
possibly damaging |
Het |
| Csf2ra |
G |
A |
19: 61,215,766 (GRCm39) |
A13V |
possibly damaging |
Het |
| Cul3 |
T |
C |
1: 80,254,139 (GRCm39) |
T546A |
possibly damaging |
Het |
| Dnah8 |
C |
T |
17: 31,013,691 (GRCm39) |
P3811S |
probably damaging |
Het |
| Dock6 |
T |
A |
9: 21,731,690 (GRCm39) |
H1173L |
probably damaging |
Het |
| Dpysl4 |
T |
C |
7: 138,676,192 (GRCm39) |
I328T |
possibly damaging |
Het |
| Dspp |
G |
A |
5: 104,323,321 (GRCm39) |
G155R |
probably damaging |
Het |
| Fbxo33 |
A |
T |
12: 59,251,545 (GRCm39) |
C57* |
probably null |
Het |
| Frrs1 |
T |
A |
3: 116,690,399 (GRCm39) |
V14D |
probably benign |
Het |
| Gm13199 |
C |
T |
2: 5,867,113 (GRCm39) |
G96S |
unknown |
Het |
| Gpr62 |
A |
T |
9: 106,342,814 (GRCm39) |
V38E |
probably damaging |
Het |
| Kcnt2 |
C |
A |
1: 140,353,104 (GRCm39) |
P271H |
probably damaging |
Het |
| Kpna3 |
C |
T |
14: 61,640,461 (GRCm39) |
V34I |
probably benign |
Het |
| Lamb1 |
C |
T |
12: 31,316,863 (GRCm39) |
Q71* |
probably null |
Het |
| Lipk |
T |
C |
19: 34,016,507 (GRCm39) |
I245T |
possibly damaging |
Het |
| Lrp1b |
T |
G |
2: 40,711,719 (GRCm39) |
N3281T |
possibly damaging |
Het |
| Lrp5 |
A |
C |
19: 3,654,094 (GRCm39) |
I1111S |
probably benign |
Het |
| Mmrn1 |
T |
C |
6: 60,964,058 (GRCm39) |
V1020A |
probably benign |
Het |
| Or4c107 |
G |
A |
2: 88,789,098 (GRCm39) |
C96Y |
possibly damaging |
Het |
| Or8b48 |
G |
A |
9: 38,493,080 (GRCm39) |
C169Y |
probably damaging |
Het |
| Or8g34 |
T |
C |
9: 39,372,787 (GRCm39) |
L17P |
probably damaging |
Het |
| Pcdha7 |
A |
T |
18: 37,108,960 (GRCm39) |
T662S |
probably damaging |
Het |
| Pcdhga6 |
A |
G |
18: 37,841,612 (GRCm39) |
D444G |
probably damaging |
Het |
| Pkd2 |
T |
G |
5: 104,646,405 (GRCm39) |
D737E |
probably damaging |
Het |
| Plcd4 |
C |
T |
1: 74,590,249 (GRCm39) |
R161W |
probably damaging |
Het |
| Prpf8 |
A |
G |
11: 75,391,734 (GRCm39) |
Y1494C |
possibly damaging |
Het |
| Rad17 |
A |
C |
13: 100,770,369 (GRCm39) |
|
probably null |
Het |
| Rbm17 |
A |
T |
2: 11,590,485 (GRCm39) |
F390Y |
probably damaging |
Het |
| Rhod |
A |
T |
19: 4,489,315 (GRCm39) |
L22Q |
probably damaging |
Het |
| Serpina1f |
T |
C |
12: 103,656,046 (GRCm39) |
D394G |
possibly damaging |
Het |
| Serpina1f |
G |
A |
12: 103,659,890 (GRCm39) |
Q131* |
probably null |
Het |
| Spocd1 |
A |
T |
4: 129,842,752 (GRCm39) |
S56C |
probably damaging |
Het |
| St7l |
G |
A |
3: 104,782,244 (GRCm39) |
R207H |
probably benign |
Het |
| Tasor |
T |
A |
14: 27,188,254 (GRCm39) |
L900* |
probably null |
Het |
| Tbx18 |
T |
C |
9: 87,595,566 (GRCm39) |
D336G |
possibly damaging |
Het |
| Tfg |
A |
T |
16: 56,514,779 (GRCm39) |
Y135* |
probably null |
Het |
| Tjp2 |
A |
G |
19: 24,073,963 (GRCm39) |
L1108P |
probably benign |
Het |
| Tmem150c |
C |
A |
5: 100,243,524 (GRCm39) |
V8L |
probably benign |
Het |
| Ttn |
A |
T |
2: 76,746,789 (GRCm39) |
H4753Q |
probably benign |
Het |
| Tyw1 |
T |
A |
5: 130,354,540 (GRCm39) |
I589N |
probably damaging |
Het |
| Usp40 |
C |
T |
1: 87,927,592 (GRCm39) |
R139Q |
probably damaging |
Het |
| Zgrf1 |
A |
G |
3: 127,378,414 (GRCm39) |
Y174C |
probably damaging |
Het |
|
| Other mutations in Gpr155 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00731:Gpr155
|
APN |
2 |
73,192,957 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01432:Gpr155
|
APN |
2 |
73,182,229 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
IGL01528:Gpr155
|
APN |
2 |
73,192,767 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01718:Gpr155
|
APN |
2 |
73,212,576 (GRCm39) |
missense |
probably benign |
|
|
IGL01733:Gpr155
|
APN |
2 |
73,183,956 (GRCm39) |
splice site |
probably null |
|
|
IGL03342:Gpr155
|
APN |
2 |
73,180,022 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT1430001:Gpr155
|
UTSW |
2 |
73,200,482 (GRCm39) |
missense |
probably benign |
0.08 |
|
PIT4810001:Gpr155
|
UTSW |
2 |
73,178,607 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0226:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0399:Gpr155
|
UTSW |
2 |
73,200,346 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R0445:Gpr155
|
UTSW |
2 |
73,200,488 (GRCm39) |
splice site |
probably benign |
|
|
R1570:Gpr155
|
UTSW |
2 |
73,200,382 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1598:Gpr155
|
UTSW |
2 |
73,200,434 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1647:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1648:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1756:Gpr155
|
UTSW |
2 |
73,197,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1760:Gpr155
|
UTSW |
2 |
73,212,279 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2033:Gpr155
|
UTSW |
2 |
73,178,526 (GRCm39) |
missense |
probably benign |
0.18 |
|
R2044:Gpr155
|
UTSW |
2 |
73,203,977 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2145:Gpr155
|
UTSW |
2 |
73,187,002 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2172:Gpr155
|
UTSW |
2 |
73,212,471 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2274:Gpr155
|
UTSW |
2 |
73,178,479 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3878:Gpr155
|
UTSW |
2 |
73,198,736 (GRCm39) |
nonsense |
probably null |
|
|
R3924:Gpr155
|
UTSW |
2 |
73,200,420 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4910:Gpr155
|
UTSW |
2 |
73,197,882 (GRCm39) |
nonsense |
probably null |
|
|
R4950:Gpr155
|
UTSW |
2 |
73,212,529 (GRCm39) |
missense |
probably benign |
|
|
R5337:Gpr155
|
UTSW |
2 |
73,178,592 (GRCm39) |
missense |
probably benign |
0.32 |
|
R5830:Gpr155
|
UTSW |
2 |
73,200,433 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R5929:Gpr155
|
UTSW |
2 |
73,204,011 (GRCm39) |
nonsense |
probably null |
|
|
R6293:Gpr155
|
UTSW |
2 |
73,204,341 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R6553:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6585:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7003:Gpr155
|
UTSW |
2 |
73,173,961 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7353:Gpr155
|
UTSW |
2 |
73,197,835 (GRCm39) |
nonsense |
probably null |
|
|
R7506:Gpr155
|
UTSW |
2 |
73,198,683 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R7631:Gpr155
|
UTSW |
2 |
73,213,291 (GRCm39) |
intron |
probably benign |
|
|
R7753:Gpr155
|
UTSW |
2 |
73,212,550 (GRCm39) |
missense |
probably benign |
0.27 |
|
R7810:Gpr155
|
UTSW |
2 |
73,212,296 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7813:Gpr155
|
UTSW |
2 |
73,212,329 (GRCm39) |
nonsense |
probably null |
|
|
R7815:Gpr155
|
UTSW |
2 |
73,192,904 (GRCm39) |
missense |
probably benign |
|
|
R7873:Gpr155
|
UTSW |
2 |
73,173,934 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R8506:Gpr155
|
UTSW |
2 |
73,173,806 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8680:Gpr155
|
UTSW |
2 |
73,174,039 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8856:Gpr155
|
UTSW |
2 |
73,203,993 (GRCm39) |
missense |
probably benign |
0.27 |
|
R8872:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9116:Gpr155
|
UTSW |
2 |
73,204,109 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
R9683:Gpr155
|
UTSW |
2 |
73,192,780 (GRCm39) |
missense |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TGGTCTCTTAGAGTACGGGC -3'
(R):5'- CCAACAGATGCTTCGTGTTTAG -3'
Sequencing Primer
(F):5'- TCTCTTAGAGTACGGGCAGGAG -3'
(R):5'- CAGATGCTTCGTGTTTAGAACAGCTC -3'
|
| Posted On |
2017-02-15 |
Incidental Mutation