Mutagenetix > Incidental Mutation

Incidental Mutation 'R5928:Usp34'

460029

Institutional Source

Beutler Lab

Gene Symbol

Usp34

Ensembl Gene

ENSMUSG00000056342

Gene Name

ubiquitin specific peptidase 34

Synonyms

Murr2, A530081C03Rik

MMRRC Submission

044123-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.803) question?

Stock #

R5928 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23256895-23440560 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 23386040 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 2156 (T2156A)

Ref Sequence

ENSEMBL: ENSMUSP00000137430 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000130131] [ENSMUST00000180046]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000130131
AA Change: T397A

PolyPhen 2 Score 0.955 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000115168
Gene: ENSMUSG00000056342
AA Change: T397A

Domain	Start	End	E-Value	Type
low complexity region	33	45	N/A	INTRINSIC
Pfam:UCH	171	514	1.1e-48	PFAM
Pfam:UCH_1	172	470	1.6e-25	PFAM
low complexity region	763	785	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000137823
AA Change: T2175A

SMART Domains

Protein: ENSMUSP00000120747
Gene: ENSMUSG00000056342
AA Change: T2175A

Domain	Start	End	E-Value	Type
low complexity region	489	500	N/A	INTRINSIC
low complexity region	530	544	N/A	INTRINSIC
low complexity region	591	610	N/A	INTRINSIC
coiled coil region	626	671	N/A	INTRINSIC
low complexity region	827	842	N/A	INTRINSIC
low complexity region	1207	1218	N/A	INTRINSIC
low complexity region	1399	1410	N/A	INTRINSIC
low complexity region	1518	1532	N/A	INTRINSIC
low complexity region	1751	1764	N/A	INTRINSIC
low complexity region	1812	1824	N/A	INTRINSIC
Pfam:UCH	1950	2293	7.6e-44	PFAM
Pfam:UCH_1	1951	2249	3.6e-22	PFAM
low complexity region	2542	2564	N/A	INTRINSIC
low complexity region	2672	2679	N/A	INTRINSIC
Blast:Drf_GBD	2943	3116	3e-53	BLAST
low complexity region	3344	3357	N/A	INTRINSIC
coiled coil region	3371	3393	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000180046
AA Change: T2156A

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000137430
Gene: ENSMUSG00000056342
AA Change: T2156A

Domain	Start	End	E-Value	Type
low complexity region	469	480	N/A	INTRINSIC
low complexity region	510	524	N/A	INTRINSIC
low complexity region	571	590	N/A	INTRINSIC
coiled coil region	607	652	N/A	INTRINSIC
low complexity region	807	822	N/A	INTRINSIC
low complexity region	1187	1198	N/A	INTRINSIC
low complexity region	1379	1390	N/A	INTRINSIC
low complexity region	1498	1512	N/A	INTRINSIC
low complexity region	1731	1744	N/A	INTRINSIC
low complexity region	1792	1804	N/A	INTRINSIC
Pfam:UCH	1930	2273	2.3e-44	PFAM
Pfam:UCH_1	1931	2229	1.1e-22	PFAM
low complexity region	2522	2544	N/A	INTRINSIC
low complexity region	2652	2659	N/A	INTRINSIC
Blast:Drf_GBD	2923	3096	2e-53	BLAST
low complexity region	3324	3337	N/A	INTRINSIC
coiled coil region	3352	3374	N/A	INTRINSIC

Meta Mutation Damage Score

0.4075

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.8%
20x: 93.3%

Validation Efficiency

97% (90/93)

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	T	A	17: 24,537,159 (GRCm39)	I475L	probably benign	Het
Abcc2	C	T	19: 43,807,797 (GRCm39)	R813W	probably damaging	Het
Adam34	T	C	8: 44,105,067 (GRCm39)	T193A	probably benign	Het
Adgb	T	C	10: 10,254,531 (GRCm39)	D1118G	probably damaging	Het
Adprh	A	C	16: 38,267,746 (GRCm39)	S180A	probably benign	Het
Atad5	A	T	11: 79,985,003 (GRCm39)	D30V	probably damaging	Het
Best3	G	A	10: 116,843,532 (GRCm39)	D303N	probably damaging	Het
Bmp2k	G	C	5: 97,235,595 (GRCm39)		probably benign	Het
Btc	A	T	5: 91,514,004 (GRCm39)	V86E	probably damaging	Het
Cacna2d4	G	T	6: 119,258,659 (GRCm39)	A582S	probably benign	Het
Carm1	A	G	9: 21,486,598 (GRCm39)		probably benign	Het
Catsperg1	A	T	7: 28,906,040 (GRCm39)	S180T	probably damaging	Het
Ccdc185	A	T	1: 182,575,047 (GRCm39)	H547Q	probably benign	Het
Ccl6	A	G	11: 83,479,658 (GRCm39)	I115T	possibly damaging	Het
Cd44	C	T	2: 102,654,648 (GRCm39)	V470M	probably damaging	Het
Cdc25a	T	C	9: 109,718,861 (GRCm39)	V354A	probably damaging	Het
Cdhr2	C	A	13: 54,881,832 (GRCm39)	Q1122K	probably benign	Het
Cep290	A	G	10: 100,387,692 (GRCm39)	K1958E	probably damaging	Het
Cfap53	C	T	18: 74,492,811 (GRCm39)	P512S	possibly damaging	Het
Chrdl2	A	T	7: 99,659,200 (GRCm39)		probably benign	Het
Cibar1	A	G	4: 12,171,919 (GRCm39)		probably benign	Het
Clec7a	A	T	6: 129,442,430 (GRCm39)	F199Y	probably damaging	Het
Cracd	A	C	5: 76,989,581 (GRCm39)		probably benign	Het
Dhx29	A	G	13: 113,101,002 (GRCm39)	K1182E	probably benign	Het
Dnah11	G	T	12: 117,878,371 (GRCm39)		probably null	Het
Dnmt3a	A	G	12: 3,916,096 (GRCm39)	S94G	possibly damaging	Het
Ecpas	A	T	4: 58,849,948 (GRCm39)	M425K	possibly damaging	Het
Egfem1	T	C	3: 29,637,077 (GRCm39)	V42A	possibly damaging	Het
Eif3e	T	A	15: 43,138,728 (GRCm39)		probably null	Het
Exosc9	T	A	3: 36,609,774 (GRCm39)		probably benign	Het
Fbxw18	T	A	9: 109,529,149 (GRCm39)	T135S	probably damaging	Het
Fbxw21	T	C	9: 108,972,893 (GRCm39)	E347G	possibly damaging	Het
Gcm2	A	G	13: 41,256,874 (GRCm39)	Y292H	probably benign	Het
Gltpd2	C	A	11: 70,410,179 (GRCm39)	Q46K	probably benign	Het
Gm6741	A	G	17: 91,544,528 (GRCm39)	Y97C	probably damaging	Het
Golgb1	T	C	16: 36,732,349 (GRCm39)	L532S	probably damaging	Het
Hdac3	C	T	18: 38,074,394 (GRCm39)		probably benign	Het
Helz2	A	T	2: 180,872,177 (GRCm39)	F2554L	possibly damaging	Het
Hmbox1	T	A	14: 65,061,122 (GRCm39)	H384L	possibly damaging	Het
Hmcn1	G	T	1: 150,474,648 (GRCm39)	D4746E	possibly damaging	Het
Il17rb	C	A	14: 29,726,232 (GRCm39)		probably null	Het
Irak2	A	T	6: 113,653,587 (GRCm39)	I252F	probably damaging	Het
Khnyn	C	T	14: 56,123,344 (GRCm39)	R33C	probably damaging	Het
Ksr1	G	A	11: 78,950,545 (GRCm39)	P20L	probably damaging	Het
Lamc3	T	C	2: 31,811,721 (GRCm39)	Y903H	probably benign	Het
Miga2	T	C	2: 30,258,875 (GRCm39)		probably benign	Het
Mroh2a	A	C	1: 88,169,340 (GRCm39)	I672L	probably benign	Het
Ncoa4	T	C	14: 31,888,678 (GRCm39)		probably null	Het
Nphp3	T	C	9: 103,912,996 (GRCm39)	Y925H	probably benign	Het
Nr2c1	T	G	10: 94,024,055 (GRCm39)	L420R	probably damaging	Het
Onecut1	A	G	9: 74,770,066 (GRCm39)	N163S	probably benign	Het
Or5ac21	G	A	16: 59,123,521 (GRCm39)	E2K	probably damaging	Het
Or5d47	T	A	2: 87,804,380 (GRCm39)	S210C	probably benign	Het
Or7e166	A	T	9: 19,625,049 (GRCm39)	T309S	probably benign	Het
Pcdhb18	G	A	18: 37,623,537 (GRCm39)	R289Q	probably benign	Het
Per2	C	T	1: 91,372,373 (GRCm39)	V234I	probably damaging	Het
Pign	A	G	1: 105,485,792 (GRCm39)	V735A	possibly damaging	Het
Plekha7	T	C	7: 115,727,809 (GRCm39)	K85R	probably benign	Het
Pnma2	C	T	14: 67,154,323 (GRCm39)	T249I	probably benign	Het
Polr2b	A	G	5: 77,493,189 (GRCm39)	D1057G	probably damaging	Het
Polrmt	A	T	10: 79,576,186 (GRCm39)	L519H	probably damaging	Het
Ptar1	T	C	19: 23,695,277 (GRCm39)	I248T	probably benign	Het
Ptprh	A	C	7: 4,576,507 (GRCm39)	L251R	probably damaging	Het
Purg	T	C	8: 33,876,980 (GRCm39)	M206T	probably benign	Het
Pwp2	A	T	10: 78,018,290 (GRCm39)	F134I	probably damaging	Het
Riok3	T	A	18: 12,286,075 (GRCm39)	H434Q	probably benign	Het
Sorbs2	A	G	8: 46,216,220 (GRCm39)	I187V	probably damaging	Het
Tbc1d2b	T	C	9: 90,101,197 (GRCm39)	I598V	probably benign	Het
Tdg	T	A	10: 82,477,204 (GRCm39)	V85E	probably benign	Het
Tfb1m	C	T	17: 3,593,422 (GRCm39)	V166I	probably benign	Het
Tmem163	A	T	1: 127,419,383 (GRCm39)	M274K	probably damaging	Het
Tpr	T	C	1: 150,303,878 (GRCm39)	I1343T	probably benign	Het
Ttn	T	A	2: 76,719,794 (GRCm39)		probably benign	Het
Tut7	G	A	13: 59,969,880 (GRCm39)	A5V	probably benign	Het
Vmn1r215	A	T	13: 23,260,487 (GRCm39)	T176S	possibly damaging	Het
Vps52	C	T	17: 34,180,100 (GRCm39)	P268L	possibly damaging	Het
Xbp1	G	A	11: 5,473,514 (GRCm39)		probably benign	Het
Ythdc2	T	A	18: 44,966,272 (GRCm39)	F169L	probably benign	Het
Zfyve16	T	C	13: 92,658,625 (GRCm39)	R429G	probably benign	Het
Zzef1	A	G	11: 72,803,678 (GRCm39)	E2504G	probably damaging	Het

Other mutations in Usp34

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL00477:Usp34	APN	11	23,418,879 (GRCm39)	missense	probably damaging	0.99
IGL01307:Usp34	APN	11	23,367,676 (GRCm39)	missense	probably damaging	0.99
IGL01313:Usp34	APN	11	23,423,206 (GRCm39)	missense	probably damaging	1.00
IGL01794:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01826:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01827:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01830:Usp34	APN	11	23,386,020 (GRCm39)	missense	probably damaging	0.98
IGL01867:Usp34	APN	11	23,334,411 (GRCm39)	missense	possibly damaging	0.77
IGL01939:Usp34	APN	11	23,295,141 (GRCm39)	splice site	probably benign
IGL01977:Usp34	APN	11	23,402,661 (GRCm39)	missense	probably damaging	1.00
IGL01985:Usp34	APN	11	23,402,565 (GRCm39)	missense	probably damaging	1.00
IGL02011:Usp34	APN	11	23,421,554 (GRCm39)	missense	probably damaging	0.99
IGL02302:Usp34	APN	11	23,417,243 (GRCm39)	missense	possibly damaging	0.91
IGL02423:Usp34	APN	11	23,304,900 (GRCm39)	missense	probably benign	0.11
IGL02491:Usp34	APN	11	23,382,630 (GRCm39)	missense	probably damaging	0.98
IGL02532:Usp34	APN	11	23,320,291 (GRCm39)	missense	probably damaging	0.99
IGL02561:Usp34	APN	11	23,301,652 (GRCm39)	missense	probably benign	0.09
IGL02706:Usp34	APN	11	23,338,659 (GRCm39)	splice site	probably benign
IGL02891:Usp34	APN	11	23,437,166 (GRCm39)	missense	probably benign	0.09
IGL03079:Usp34	APN	11	23,382,247 (GRCm39)	missense	possibly damaging	0.48
IGL03089:Usp34	APN	11	23,396,958 (GRCm39)	missense	possibly damaging	0.84
IGL03175:Usp34	APN	11	23,438,686 (GRCm39)	missense	probably benign
IGL03256:Usp34	APN	11	23,370,090 (GRCm39)	nonsense	probably null
IGL03280:Usp34	APN	11	23,304,897 (GRCm39)	missense	probably damaging	1.00
IGL03289:Usp34	APN	11	23,343,818 (GRCm39)	missense	possibly damaging	0.94
IGL03408:Usp34	APN	11	23,396,957 (GRCm39)	missense	possibly damaging	0.92
Chub	UTSW	11	23,414,686 (GRCm39)	missense	probably damaging	0.99
Cicione	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R5571_Usp34_680	UTSW	11	23,407,975 (GRCm39)	missense	probably damaging	0.99
R5713_Usp34_003	UTSW	11	23,293,515 (GRCm39)	missense	possibly damaging	0.94
Roebuck	UTSW	11	23,436,810 (GRCm39)	splice site	probably benign
stoat	UTSW	11	23,437,203 (GRCm39)	missense
tunnelvision	UTSW	11	23,396,968 (GRCm39)	missense
I2288:Usp34	UTSW	11	23,382,473 (GRCm39)	splice site	probably benign
R0047:Usp34	UTSW	11	23,414,403 (GRCm39)	missense	probably benign	0.34
R0047:Usp34	UTSW	11	23,414,403 (GRCm39)	missense	probably benign	0.34
R0099:Usp34	UTSW	11	23,313,111 (GRCm39)	missense	probably damaging	1.00
R0240:Usp34	UTSW	11	23,383,206 (GRCm39)	missense	probably damaging	0.99
R0240:Usp34	UTSW	11	23,383,206 (GRCm39)	missense	probably damaging	0.99
R0403:Usp34	UTSW	11	23,283,838 (GRCm39)	missense	possibly damaging	0.82
R0432:Usp34	UTSW	11	23,351,505 (GRCm39)	missense	probably damaging	0.99
R0446:Usp34	UTSW	11	23,417,207 (GRCm39)	missense	probably damaging	0.97
R0455:Usp34	UTSW	11	23,396,741 (GRCm39)	splice site	probably benign
R0470:Usp34	UTSW	11	23,386,001 (GRCm39)	missense	possibly damaging	0.94
R0472:Usp34	UTSW	11	23,334,509 (GRCm39)	splice site	probably benign
R0512:Usp34	UTSW	11	23,401,997 (GRCm39)	missense	probably benign	0.04
R0557:Usp34	UTSW	11	23,353,848 (GRCm39)	missense	probably damaging	0.98
R0562:Usp34	UTSW	11	23,382,406 (GRCm39)	splice site	probably benign
R0656:Usp34	UTSW	11	23,422,967 (GRCm39)	missense	probably damaging	0.99
R0693:Usp34	UTSW	11	23,402,637 (GRCm39)	missense	probably damaging	0.97
R0739:Usp34	UTSW	11	23,417,243 (GRCm39)	missense	possibly damaging	0.91
R1061:Usp34	UTSW	11	23,334,420 (GRCm39)	missense	possibly damaging	0.51
R1078:Usp34	UTSW	11	23,383,175 (GRCm39)	splice site	probably benign
R1223:Usp34	UTSW	11	23,396,464 (GRCm39)	splice site	probably null
R1295:Usp34	UTSW	11	23,334,477 (GRCm39)	missense	probably damaging	1.00
R1430:Usp34	UTSW	11	23,409,151 (GRCm39)	missense	probably damaging	0.97
R1445:Usp34	UTSW	11	23,301,629 (GRCm39)	missense	probably damaging	0.99
R1468:Usp34	UTSW	11	23,391,171 (GRCm39)	missense	probably damaging	1.00
R1468:Usp34	UTSW	11	23,391,171 (GRCm39)	missense	probably damaging	1.00
R1471:Usp34	UTSW	11	23,438,862 (GRCm39)	missense	probably benign	0.20
R1475:Usp34	UTSW	11	23,423,253 (GRCm39)	missense	probably damaging	0.99
R1628:Usp34	UTSW	11	23,438,725 (GRCm39)	missense	probably damaging	1.00
R1631:Usp34	UTSW	11	23,410,651 (GRCm39)	missense	probably damaging	0.99
R1655:Usp34	UTSW	11	23,325,051 (GRCm39)	missense	probably benign	0.05
R1741:Usp34	UTSW	11	23,314,103 (GRCm39)	missense	probably benign	0.00
R1854:Usp34	UTSW	11	23,376,153 (GRCm39)	missense	probably benign	0.24
R1867:Usp34	UTSW	11	23,311,593 (GRCm39)	missense	possibly damaging	0.82
R1869:Usp34	UTSW	11	23,314,479 (GRCm39)	missense	probably benign	0.37
R1870:Usp34	UTSW	11	23,314,479 (GRCm39)	missense	probably benign	0.37
R1871:Usp34	UTSW	11	23,314,479 (GRCm39)	missense	probably benign	0.37
R1967:Usp34	UTSW	11	23,314,503 (GRCm39)	missense	probably benign	0.01
R2051:Usp34	UTSW	11	23,414,468 (GRCm39)	missense	probably damaging	0.97
R2132:Usp34	UTSW	11	23,414,556 (GRCm39)	missense	possibly damaging	0.95
R2156:Usp34	UTSW	11	23,332,602 (GRCm39)	missense	probably damaging	0.98
R2205:Usp34	UTSW	11	23,335,147 (GRCm39)	missense	probably damaging	0.97
R2342:Usp34	UTSW	11	23,353,599 (GRCm39)	missense	possibly damaging	0.46
R3431:Usp34	UTSW	11	23,320,466 (GRCm39)	missense	possibly damaging	0.95
R3812:Usp34	UTSW	11	23,414,517 (GRCm39)	missense	possibly damaging	0.94
R3872:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3873:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3874:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3875:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R3925:Usp34	UTSW	11	23,293,640 (GRCm39)	missense	probably benign	0.28
R3972:Usp34	UTSW	11	23,407,803 (GRCm39)	missense	probably damaging	1.00
R4018:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R4042:Usp34	UTSW	11	23,439,033 (GRCm39)	missense	possibly damaging	0.85
R4155:Usp34	UTSW	11	23,367,676 (GRCm39)	missense	probably damaging	0.99
R4197:Usp34	UTSW	11	23,394,189 (GRCm39)	missense	probably damaging	0.98
R4352:Usp34	UTSW	11	23,270,727 (GRCm39)	missense	possibly damaging	0.73
R4379:Usp34	UTSW	11	23,334,499 (GRCm39)	missense	possibly damaging	0.52
R4444:Usp34	UTSW	11	23,385,998 (GRCm39)	missense	probably damaging	0.98
R4475:Usp34	UTSW	11	23,407,975 (GRCm39)	missense	possibly damaging	0.95
R4501:Usp34	UTSW	11	23,351,529 (GRCm39)	missense	probably damaging	1.00
R4527:Usp34	UTSW	11	23,371,257 (GRCm39)	missense	possibly damaging	0.57
R4603:Usp34	UTSW	11	23,414,633 (GRCm39)	missense	probably damaging	0.97
R4612:Usp34	UTSW	11	23,382,268 (GRCm39)	missense	probably damaging	0.99
R4673:Usp34	UTSW	11	23,314,480 (GRCm39)	small deletion	probably benign
R4707:Usp34	UTSW	11	23,437,215 (GRCm39)	missense	probably damaging	1.00
R4736:Usp34	UTSW	11	23,343,749 (GRCm39)	splice site	probably null
R4867:Usp34	UTSW	11	23,401,999 (GRCm39)	missense	probably benign	0.28
R4879:Usp34	UTSW	11	23,323,410 (GRCm39)	missense	possibly damaging	0.94
R4977:Usp34	UTSW	11	23,438,982 (GRCm39)	missense	probably damaging	1.00
R5004:Usp34	UTSW	11	23,414,586 (GRCm39)	missense	probably damaging	1.00
R5057:Usp34	UTSW	11	23,408,086 (GRCm39)	intron	probably benign
R5068:Usp34	UTSW	11	23,410,665 (GRCm39)	missense	possibly damaging	0.94
R5304:Usp34	UTSW	11	23,293,616 (GRCm39)	missense	probably damaging	1.00
R5320:Usp34	UTSW	11	23,283,739 (GRCm39)	missense	probably benign
R5327:Usp34	UTSW	11	23,418,846 (GRCm39)	missense	probably damaging	1.00
R5328:Usp34	UTSW	11	23,438,659 (GRCm39)	missense	probably benign	0.04
R5328:Usp34	UTSW	11	23,414,616 (GRCm39)	missense	probably benign	0.01
R5390:Usp34	UTSW	11	23,394,202 (GRCm39)	critical splice donor site	probably null
R5434:Usp34	UTSW	11	23,362,271 (GRCm39)	missense	probably damaging	0.99
R5523:Usp34	UTSW	11	23,299,198 (GRCm39)	missense	probably benign	0.39
R5567:Usp34	UTSW	11	23,438,336 (GRCm39)	missense	probably damaging	0.97
R5571:Usp34	UTSW	11	23,407,975 (GRCm39)	missense	probably damaging	0.99
R5645:Usp34	UTSW	11	23,325,024 (GRCm39)	missense	possibly damaging	0.86
R5713:Usp34	UTSW	11	23,293,515 (GRCm39)	missense	possibly damaging	0.94
R5719:Usp34	UTSW	11	23,304,846 (GRCm39)	missense	probably benign	0.00
R5813:Usp34	UTSW	11	23,371,340 (GRCm39)	missense	probably benign	0.38
R5921:Usp34	UTSW	11	23,414,686 (GRCm39)	missense	probably damaging	0.99
R5944:Usp34	UTSW	11	23,313,089 (GRCm39)	missense	probably damaging	1.00
R6198:Usp34	UTSW	11	23,434,127 (GRCm39)	missense	probably damaging	1.00
R6229:Usp34	UTSW	11	23,396,778 (GRCm39)	missense	probably damaging	0.99
R6306:Usp34	UTSW	11	23,362,260 (GRCm39)	missense	possibly damaging	0.94
R6320:Usp34	UTSW	11	23,402,520 (GRCm39)	missense	probably damaging	0.98
R6341:Usp34	UTSW	11	23,331,353 (GRCm39)	missense	probably damaging	0.97
R6374:Usp34	UTSW	11	23,388,914 (GRCm39)	missense	probably damaging	1.00
R6398:Usp34	UTSW	11	23,438,666 (GRCm39)	missense	probably benign
R6438:Usp34	UTSW	11	23,314,266 (GRCm39)	missense	probably benign	0.02
R6668:Usp34	UTSW	11	23,410,659 (GRCm39)	missense	probably damaging	0.97
R6700:Usp34	UTSW	11	23,389,011 (GRCm39)	missense	probably damaging	1.00
R6783:Usp34	UTSW	11	23,362,318 (GRCm39)	missense	probably damaging	1.00
R6821:Usp34	UTSW	11	23,317,491 (GRCm39)	missense	possibly damaging	0.79
R6855:Usp34	UTSW	11	23,402,569 (GRCm39)	missense	possibly damaging	0.94
R6916:Usp34	UTSW	11	23,408,023 (GRCm39)	missense	probably damaging	0.98
R7020:Usp34	UTSW	11	23,343,954 (GRCm39)	missense	probably benign	0.05
R7026:Usp34	UTSW	11	23,311,622 (GRCm39)	missense	probably damaging	1.00
R7085:Usp34	UTSW	11	23,313,097 (GRCm39)	missense
R7101:Usp34	UTSW	11	23,376,183 (GRCm39)	missense
R7168:Usp34	UTSW	11	23,414,585 (GRCm39)	missense
R7192:Usp34	UTSW	11	23,410,571 (GRCm39)	missense
R7264:Usp34	UTSW	11	23,283,566 (GRCm39)	missense	probably benign	0.00
R7325:Usp34	UTSW	11	23,369,052 (GRCm39)	missense
R7343:Usp34	UTSW	11	23,438,868 (GRCm39)	missense
R7358:Usp34	UTSW	11	23,311,683 (GRCm39)	missense	probably damaging	0.99
R7369:Usp34	UTSW	11	23,382,361 (GRCm39)	missense
R7389:Usp34	UTSW	11	23,295,200 (GRCm39)	missense
R7459:Usp34	UTSW	11	23,314,458 (GRCm39)	missense	possibly damaging	0.53
R7517:Usp34	UTSW	11	23,396,968 (GRCm39)	missense
R7729:Usp34	UTSW	11	23,399,268 (GRCm39)	missense
R7777:Usp34	UTSW	11	23,332,638 (GRCm39)	missense
R7810:Usp34	UTSW	11	23,362,314 (GRCm39)	missense
R7836:Usp34	UTSW	11	23,396,614 (GRCm39)	missense
R7862:Usp34	UTSW	11	23,414,718 (GRCm39)	missense
R7993:Usp34	UTSW	11	23,327,622 (GRCm39)	missense
R8050:Usp34	UTSW	11	23,396,787 (GRCm39)	missense
R8054:Usp34	UTSW	11	23,311,295 (GRCm39)	missense
R8239:Usp34	UTSW	11	23,396,750 (GRCm39)	missense
R8266:Usp34	UTSW	11	23,436,810 (GRCm39)	splice site	probably benign
R8347:Usp34	UTSW	11	23,362,345 (GRCm39)	missense
R8409:Usp34	UTSW	11	23,407,811 (GRCm39)	missense
R8692:Usp34	UTSW	11	23,379,325 (GRCm39)	missense
R8694:Usp34	UTSW	11	23,434,161 (GRCm39)	missense
R8734:Usp34	UTSW	11	23,394,184 (GRCm39)	missense
R8806:Usp34	UTSW	11	23,434,143 (GRCm39)	missense
R8914:Usp34	UTSW	11	23,293,604 (GRCm39)	missense
R8987:Usp34	UTSW	11	23,414,267 (GRCm39)	missense
R9013:Usp34	UTSW	11	23,320,302 (GRCm39)	missense
R9108:Usp34	UTSW	11	23,320,528 (GRCm39)	missense
R9264:Usp34	UTSW	11	23,439,064 (GRCm39)	missense
R9301:Usp34	UTSW	11	23,422,951 (GRCm39)	missense
R9375:Usp34	UTSW	11	23,437,203 (GRCm39)	missense
R9385:Usp34	UTSW	11	23,399,223 (GRCm39)	missense
R9500:Usp34	UTSW	11	23,331,337 (GRCm39)	missense	probably damaging	0.99
R9566:Usp34	UTSW	11	23,317,529 (GRCm39)	missense
R9629:Usp34	UTSW	11	23,314,364 (GRCm39)	missense
R9679:Usp34	UTSW	11	23,394,369 (GRCm39)	missense
R9680:Usp34	UTSW	11	23,317,385 (GRCm39)	missense	possibly damaging	0.94
R9686:Usp34	UTSW	11	23,424,351 (GRCm39)	missense
R9752:Usp34	UTSW	11	23,409,182 (GRCm39)	missense	probably benign	0.11
X0023:Usp34	UTSW	11	23,325,028 (GRCm39)	missense	possibly damaging	0.73
X0057:Usp34	UTSW	11	23,407,824 (GRCm39)	missense	possibly damaging	0.86
Z1176:Usp34	UTSW	11	23,423,221 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CACCCCAGTAGAAAATTACGTG -3'
(R):5'- GTGCACTAGTCTGTCATATGATTC -3'

Sequencing Primer
(F):5'- CCCAGTAGAAAATTACGTGACTTAAC -3'
(R):5'- CCATCTGTAACTCCAGTTGAGAGG -3'

Posted On

2017-02-28