Incidental Mutation 'R5909:Atp1a2'
ID 460823
Institutional Source Beutler Lab
Gene Symbol Atp1a2
Ensembl Gene ENSMUSG00000007097
Gene Name ATPase, Na+/K+ transporting, alpha 2 polypeptide
Synonyms Atpa-3
MMRRC Submission 044106-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R5909 (G1)
Quality Score 225
Status Not validated
Chromosome 1
Chromosomal Location 172099276-172125631 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 172114797 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Lysine at position 329 (N329K)
Ref Sequence ENSEMBL: ENSMUSP00000095072 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000085913] [ENSMUST00000097464] [ENSMUST00000139528]
AlphaFold Q6PIE5
Predicted Effect probably damaging
Transcript: ENSMUST00000085913
AA Change: N329K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000083077
Gene: ENSMUSG00000007097
AA Change: N329K

DomainStartEndE-ValueType
low complexity region 22 36 N/A INTRINSIC
Cation_ATPase_N 40 114 5.28e-19 SMART
Pfam:E1-E2_ATPase 132 363 2.5e-59 PFAM
Pfam:Hydrolase 368 726 4.5e-19 PFAM
Pfam:HAD 371 723 3.2e-18 PFAM
Pfam:Cation_ATPase 424 518 1.9e-25 PFAM
Pfam:Cation_ATPase_C 796 1005 1.4e-45 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000097464
AA Change: N329K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000095072
Gene: ENSMUSG00000007097
AA Change: N329K

DomainStartEndE-ValueType
low complexity region 22 36 N/A INTRINSIC
Cation_ATPase_N 40 114 5.28e-19 SMART
Pfam:E1-E2_ATPase 133 364 1.9e-63 PFAM
Pfam:Hydrolase 368 726 2e-32 PFAM
Pfam:HAD 371 723 1.7e-15 PFAM
Pfam:Hydrolase_like2 424 518 1.3e-26 PFAM
Pfam:Cation_ATPase_C 796 947 3.2e-30 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000131751
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137679
SMART Domains Protein: ENSMUSP00000117873
Gene: ENSMUSG00000007097

DomainStartEndE-ValueType
low complexity region 22 36 N/A INTRINSIC
Cation_ATPase_N 40 114 5.28e-19 SMART
Pfam:E1-E2_ATPase 133 364 1.2e-63 PFAM
Pfam:Hydrolase 368 613 8.5e-9 PFAM
Pfam:Hydrolase_like2 424 518 5.7e-27 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000139528
SMART Domains Protein: ENSMUSP00000134280
Gene: ENSMUSG00000038034

DomainStartEndE-ValueType
IG_like 19 84 3.66e1 SMART
low complexity region 92 103 N/A INTRINSIC
IG 106 222 2.3e-3 SMART
IG 246 370 9.49e-5 SMART
IG 382 508 3.59e-5 SMART
transmembrane domain 515 537 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000155363
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.8%
  • 20x: 93.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008]
PHENOTYPE: Homozygous mutants die immediately after birth from breathing failure, lack spontaneous respiratory rhythm activity, have elevated levels of extracellular GABA in the brain, and have abnormal chloride homeostasis in brainstem neurons. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamts8 T C 9: 30,873,224 (GRCm39) S810P probably benign Het
Adcy7 A T 8: 89,052,124 (GRCm39) I931F probably damaging Het
Alcam G T 16: 52,111,356 (GRCm39) Q248K probably benign Het
Astn1 A C 1: 158,429,507 (GRCm39) R750S probably damaging Het
Bcl6 A G 16: 23,791,556 (GRCm39) V266A probably benign Het
Bdp1 A G 13: 100,228,794 (GRCm39) V278A probably benign Het
Ccdc162 T C 10: 41,437,111 (GRCm39) E493G probably damaging Het
Cpvl T C 6: 53,909,413 (GRCm39) Y241C probably damaging Het
Ddx55 T C 5: 124,704,913 (GRCm39) M390T probably benign Het
Ero1b A G 13: 12,594,139 (GRCm39) E102G probably benign Het
Etnk1 A G 6: 143,143,164 (GRCm39) D273G probably benign Het
Exoc3 A G 13: 74,347,643 (GRCm39) V109A probably damaging Het
F830016B08Rik T A 18: 60,433,091 (GRCm39) I58N probably damaging Het
Fancd2 T A 6: 113,538,672 (GRCm39) V589E probably benign Het
Fbll1 G A 11: 35,689,159 (GRCm39) R35C unknown Het
Fnbp1 A G 2: 30,938,211 (GRCm39) probably null Het
Glce G T 9: 61,967,426 (GRCm39) A575D probably damaging Het
Ift20 T A 11: 78,430,867 (GRCm39) M70K possibly damaging Het
Impg2 T C 16: 56,078,499 (GRCm39) V487A probably damaging Het
Isx A G 8: 75,619,426 (GRCm39) D206G probably benign Het
Jak3 A G 8: 72,136,875 (GRCm39) I684V possibly damaging Het
Kdm5d G A Y: 941,306 (GRCm39) S1169N probably benign Het
Krt35 A T 11: 99,986,639 (GRCm39) L125Q probably damaging Het
Lama4 G A 10: 38,948,855 (GRCm39) A873T probably benign Het
Lrch4 T C 5: 137,632,127 (GRCm39) S74P possibly damaging Het
Maneal A T 4: 124,750,966 (GRCm39) Y263* probably null Het
Mcat C T 15: 83,432,116 (GRCm39) A251T probably benign Het
Mup3 T G 4: 62,004,244 (GRCm39) T90P probably benign Het
Mybpc2 G A 7: 44,156,515 (GRCm39) A812V probably damaging Het
Naa15 T C 3: 51,367,485 (GRCm39) F503L probably damaging Het
Nudt8 T C 19: 4,050,727 (GRCm39) L25S possibly damaging Het
Oas1e A T 5: 120,926,972 (GRCm39) V245D probably damaging Het
Ofcc1 A G 13: 40,417,054 (GRCm39) M109T possibly damaging Het
Or4c58 A G 2: 89,674,735 (GRCm39) V194A possibly damaging Het
Or4p18 A T 2: 88,232,535 (GRCm39) F248I probably damaging Het
Phkb G T 8: 86,748,076 (GRCm39) probably null Het
Pidd1 A C 7: 141,021,183 (GRCm39) L365R probably damaging Het
Pira12 G A 7: 3,900,621 (GRCm39) T43I probably damaging Het
Pkd1l2 G A 8: 117,750,795 (GRCm39) R1739C probably benign Het
Pkdrej A T 15: 85,702,497 (GRCm39) D1146E possibly damaging Het
Pkhd1l1 G T 15: 44,390,159 (GRCm39) W1425L probably damaging Het
Plxna4 A G 6: 32,494,181 (GRCm39) L145P probably damaging Het
Plxnd1 G T 6: 115,945,649 (GRCm39) D941E probably benign Het
Prdm13 G T 4: 21,683,894 (GRCm39) Q126K unknown Het
Rbm25 T A 12: 83,728,362 (GRCm39) V837E probably damaging Het
Rcc2 T A 4: 140,444,379 (GRCm39) Y357N probably damaging Het
Siglec1 A T 2: 130,919,884 (GRCm39) N882K probably damaging Het
Sox13 A G 1: 133,311,627 (GRCm39) I535T probably benign Het
Srrm2 T C 17: 24,040,291 (GRCm39) S2408P probably benign Het
Stat3 G A 11: 100,794,556 (GRCm39) T251I probably benign Het
Sulf1 A T 1: 12,929,039 (GRCm39) D102V possibly damaging Het
Tbc1d2 C T 4: 46,629,912 (GRCm39) G252R probably benign Het
Tmem145 G T 7: 25,007,618 (GRCm39) L208F possibly damaging Het
Trim17 A T 11: 58,859,506 (GRCm39) E240V probably damaging Het
Trim43b A T 9: 88,967,451 (GRCm39) I395K possibly damaging Het
Trmt61a C A 12: 111,647,292 (GRCm39) H130N probably damaging Het
Unc5b A C 10: 60,608,138 (GRCm39) L654R probably damaging Het
Vmn1r66 A T 7: 10,008,269 (GRCm39) S255T probably benign Het
Ylpm1 C T 12: 85,087,660 (GRCm39) P1148L probably damaging Het
Zbtb10 T C 3: 9,345,109 (GRCm39) F677S probably benign Het
Zfp493 T A 13: 67,934,717 (GRCm39) C223* probably null Het
Other mutations in Atp1a2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00484:Atp1a2 APN 1 172,103,569 (GRCm39) missense probably damaging 1.00
IGL00954:Atp1a2 APN 1 172,118,201 (GRCm39) missense probably damaging 1.00
IGL01083:Atp1a2 APN 1 172,112,186 (GRCm39) missense probably benign
IGL01372:Atp1a2 APN 1 172,106,510 (GRCm39) missense probably damaging 1.00
IGL01762:Atp1a2 APN 1 172,112,480 (GRCm39) missense possibly damaging 0.89
IGL01896:Atp1a2 APN 1 172,113,578 (GRCm39) missense probably damaging 1.00
IGL01942:Atp1a2 APN 1 172,113,876 (GRCm39) missense probably benign 0.35
IGL01944:Atp1a2 APN 1 172,103,754 (GRCm39) missense probably damaging 0.98
IGL02219:Atp1a2 APN 1 172,107,298 (GRCm39) nonsense probably null
IGL02219:Atp1a2 APN 1 172,107,285 (GRCm39) missense probably damaging 1.00
IGL02304:Atp1a2 APN 1 172,116,920 (GRCm39) missense probably benign
IGL02507:Atp1a2 APN 1 172,113,338 (GRCm39) missense probably damaging 1.00
IGL02557:Atp1a2 APN 1 172,106,218 (GRCm39) missense possibly damaging 0.83
IGL02632:Atp1a2 APN 1 172,108,181 (GRCm39) missense possibly damaging 0.89
IGL03053:Atp1a2 APN 1 172,105,923 (GRCm39) missense probably damaging 1.00
IGL03104:Atp1a2 APN 1 172,120,934 (GRCm39) missense probably damaging 0.97
IGL03161:Atp1a2 APN 1 172,106,429 (GRCm39) intron probably benign
IGL03218:Atp1a2 APN 1 172,116,870 (GRCm39) missense probably null 0.82
PIT4151001:Atp1a2 UTSW 1 172,118,288 (GRCm39) missense probably damaging 0.99
PIT4520001:Atp1a2 UTSW 1 172,106,941 (GRCm39) missense probably benign 0.00
R0121:Atp1a2 UTSW 1 172,116,909 (GRCm39) missense probably damaging 0.99
R0630:Atp1a2 UTSW 1 172,118,842 (GRCm39) missense possibly damaging 0.78
R0682:Atp1a2 UTSW 1 172,112,164 (GRCm39) missense probably benign 0.00
R0755:Atp1a2 UTSW 1 172,116,948 (GRCm39) missense probably benign 0.37
R1413:Atp1a2 UTSW 1 172,106,911 (GRCm39) missense probably damaging 1.00
R1680:Atp1a2 UTSW 1 172,106,521 (GRCm39) missense probably damaging 0.99
R2094:Atp1a2 UTSW 1 172,115,000 (GRCm39) missense probably damaging 1.00
R3714:Atp1a2 UTSW 1 172,106,551 (GRCm39) missense probably damaging 0.96
R4573:Atp1a2 UTSW 1 172,106,204 (GRCm39) missense possibly damaging 0.75
R4928:Atp1a2 UTSW 1 172,105,954 (GRCm39) missense possibly damaging 0.93
R4953:Atp1a2 UTSW 1 172,119,009 (GRCm39) intron probably benign
R5014:Atp1a2 UTSW 1 172,112,438 (GRCm39) missense probably benign 0.05
R5080:Atp1a2 UTSW 1 172,112,012 (GRCm39) intron probably benign
R5129:Atp1a2 UTSW 1 172,103,522 (GRCm39) missense probably benign 0.02
R5360:Atp1a2 UTSW 1 172,106,436 (GRCm39) critical splice donor site probably null
R5619:Atp1a2 UTSW 1 172,106,948 (GRCm39) missense probably damaging 0.99
R5622:Atp1a2 UTSW 1 172,118,994 (GRCm39) intron probably benign
R5718:Atp1a2 UTSW 1 172,107,009 (GRCm39) missense probably damaging 1.00
R5729:Atp1a2 UTSW 1 172,120,938 (GRCm39) missense probably damaging 0.99
R6018:Atp1a2 UTSW 1 172,125,579 (GRCm39) intron probably benign
R6145:Atp1a2 UTSW 1 172,114,805 (GRCm39) missense probably damaging 1.00
R6164:Atp1a2 UTSW 1 172,106,459 (GRCm39) missense probably damaging 0.97
R6315:Atp1a2 UTSW 1 172,116,903 (GRCm39) missense probably damaging 0.99
R6317:Atp1a2 UTSW 1 172,116,903 (GRCm39) missense probably damaging 0.99
R6319:Atp1a2 UTSW 1 172,116,903 (GRCm39) missense probably damaging 0.99
R6323:Atp1a2 UTSW 1 172,116,903 (GRCm39) missense probably damaging 0.99
R6324:Atp1a2 UTSW 1 172,116,903 (GRCm39) missense probably damaging 0.99
R6374:Atp1a2 UTSW 1 172,116,942 (GRCm39) missense probably damaging 1.00
R6764:Atp1a2 UTSW 1 172,112,181 (GRCm39) missense probably benign
R6812:Atp1a2 UTSW 1 172,112,444 (GRCm39) missense probably benign 0.20
R7025:Atp1a2 UTSW 1 172,112,117 (GRCm39) nonsense probably null
R7194:Atp1a2 UTSW 1 172,108,194 (GRCm39) nonsense probably null
R7459:Atp1a2 UTSW 1 172,114,862 (GRCm39) missense probably benign 0.00
R7791:Atp1a2 UTSW 1 172,103,782 (GRCm39) missense probably benign 0.28
R7889:Atp1a2 UTSW 1 172,105,631 (GRCm39) splice site probably null
R7993:Atp1a2 UTSW 1 172,118,878 (GRCm39) missense possibly damaging 0.86
R8183:Atp1a2 UTSW 1 172,116,918 (GRCm39) missense probably damaging 0.96
R8434:Atp1a2 UTSW 1 172,112,179 (GRCm39) missense probably benign 0.01
R8712:Atp1a2 UTSW 1 172,103,547 (GRCm39) missense probably benign 0.05
R8724:Atp1a2 UTSW 1 172,106,945 (GRCm39) missense probably benign 0.13
R8887:Atp1a2 UTSW 1 172,113,222 (GRCm39) missense probably null 0.02
R8965:Atp1a2 UTSW 1 172,107,612 (GRCm39) missense probably benign 0.25
R9322:Atp1a2 UTSW 1 172,107,625 (GRCm39) missense possibly damaging 0.94
R9383:Atp1a2 UTSW 1 172,107,334 (GRCm39) missense probably benign
R9451:Atp1a2 UTSW 1 172,103,494 (GRCm39) missense probably benign
R9485:Atp1a2 UTSW 1 172,105,822 (GRCm39) makesense probably null
R9727:Atp1a2 UTSW 1 172,118,936 (GRCm39) missense probably benign 0.01
Z1176:Atp1a2 UTSW 1 172,107,321 (GRCm39) missense possibly damaging 0.90
Z1177:Atp1a2 UTSW 1 172,114,903 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCCCTGAACAATTTCTGCATAC -3'
(R):5'- AGGGGCATTGTGATTGCCAC -3'

Sequencing Primer
(F):5'- GCATACGGCATCTGCTATGTGAC -3'
(R):5'- TGATTGCCACAGGTGACC -3'
Posted On 2017-02-28