Mutagenetix > Incidental Mutation

Incidental Mutation 'R5917:Zfp963'

461398

Institutional Source

Beutler Lab

Gene Symbol

Zfp963

Ensembl Gene

ENSMUSG00000092260

Gene Name

zinc finger protein 963

Synonyms

MMRRC Submission

044114-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R5917 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

70194289-70202612 bp(-) (GRCm39)

Type of Mutation

splice site (2 bp from exon)

DNA Base Change (assembly)

A to T at 70195510 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000123252 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000130458] [ENSMUST00000149782] [ENSMUST00000154063]

AlphaFold

D3Z2T8

Predicted Effect

probably benign
Transcript: ENSMUST00000126915

SMART Domains

Protein: ENSMUSP00000122850
Gene: ENSMUSG00000092544

Domain	Start	End	E-Value	Type
KRAB	16	73	1.96e-17	SMART
PDB:2I13\|B	104	210	3e-6	PDB
SCOP:d1fgja_	114	216	2e-6	SMART
Blast:KRAB	189	209	3e-6	BLAST

Predicted Effect

probably damaging
Transcript: ENSMUST00000130458
AA Change: H314Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000119087
Gene: ENSMUSG00000092260
AA Change: H314Q

Domain	Start	End	E-Value	Type
KRAB	4	61	1.96e-17	SMART
ZnF_C2H2	162	184	7.37e1	SMART
ZnF_C2H2	213	235	1.69e-3	SMART
ZnF_C2H2	240	262	8.47e-4	SMART
ZnF_C2H2	268	290	7.15e-2	SMART
ZnF_C2H2	296	318	3.83e-2	SMART
ZnF_C2H2	324	346	4.17e-3	SMART
ZnF_C2H2	352	374	5.99e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000149782

SMART Domains

Protein: ENSMUSP00000123252
Gene: ENSMUSG00000092544

Domain	Start	End	E-Value	Type
KRAB	80	139	1.2e-17	SMART
ZnF_C2H2	155	177	1.69e-3	SMART
ZnF_C2H2	182	204	8.47e-4	SMART
ZnF_C2H2	210	232	7.15e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000154063

SMART Domains

Protein: ENSMUSP00000117867
Gene: ENSMUSG00000092260

Domain	Start	End	E-Value	Type
KRAB	4	61	1.96e-17	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	T	A	12: 118,832,516 (GRCm39)	R1153*	probably null	Het
Amdhd1	T	C	10: 93,360,332 (GRCm39)	H409R	possibly damaging	Het
Anks1b	C	T	10: 90,412,803 (GRCm39)		probably benign	Het
Ascc2	T	C	11: 4,631,506 (GRCm39)	L649P	probably benign	Het
Chst15	A	G	7: 131,872,246 (GRCm39)	F12L	probably benign	Het
Clec4a4	A	T	6: 122,981,017 (GRCm39)	K83N	probably benign	Het
Comp	T	A	8: 70,829,011 (GRCm39)		probably null	Het
Cryz	T	A	3: 154,327,403 (GRCm39)	S144T	probably benign	Het
Ctss	A	G	3: 95,450,424 (GRCm39)	D125G	probably benign	Het
Dact1	G	T	12: 71,365,456 (GRCm39)	V746L	possibly damaging	Het
Dhx29	A	G	13: 113,099,377 (GRCm39)	H1134R	probably damaging	Het
Dlgap4	A	G	2: 156,546,460 (GRCm39)	D376G	probably damaging	Het
Dnah3	T	A	7: 119,615,749 (GRCm39)	H1660L	probably damaging	Het
Ep300	T	A	15: 81,512,808 (GRCm39)		probably benign	Het
Fbxo30	A	T	10: 11,165,262 (GRCm39)		probably null	Het
Fcrl2	T	A	3: 87,164,094 (GRCm39)	H345L	probably damaging	Het
Galnt11	C	A	5: 25,452,670 (GRCm39)		probably null	Het
Il31ra	A	G	13: 112,682,846 (GRCm39)	C87R	probably benign	Het
Itga4	A	T	2: 79,117,442 (GRCm39)	Q416L	probably damaging	Het
Kcnt2	A	T	1: 140,461,666 (GRCm39)	T806S	probably damaging	Het
Lama2	A	G	10: 27,066,693 (GRCm39)	S1063P	probably damaging	Het
Lama4	T	A	10: 38,924,028 (GRCm39)	S479T	probably benign	Het
Lgi4	C	T	7: 30,759,603 (GRCm39)	T53M	possibly damaging	Het
Limk1	A	G	5: 134,686,789 (GRCm39)	F533L	probably damaging	Het
Loxl1	A	G	9: 58,220,006 (GRCm39)	L55P	probably damaging	Het
Map1a	A	G	2: 121,135,697 (GRCm39)	E1933G	probably damaging	Het
Matn2	T	A	15: 34,409,912 (GRCm39)	C447*	probably null	Het
Mmrn1	T	C	6: 60,950,134 (GRCm39)		probably null	Het
Or2a7	C	T	6: 43,151,646 (GRCm39)	S242F	probably damaging	Het
Or4a66	A	T	2: 88,531,049 (GRCm39)	I208N	possibly damaging	Het
Or4d1	A	T	11: 87,805,215 (GRCm39)	N172K	probably damaging	Het
Otor	A	G	2: 142,920,431 (GRCm39)	I4M	probably benign	Het
P2rx3	A	T	2: 84,865,591 (GRCm39)	V18E	probably damaging	Het
Pcdh7	T	C	5: 57,879,097 (GRCm39)	V884A	probably damaging	Het
Pcdhb4	T	A	18: 37,442,619 (GRCm39)	V643D	probably damaging	Het
Pelo	A	G	13: 115,225,930 (GRCm39)	S176P	possibly damaging	Het
Ppp2r3d	A	T	9: 101,089,183 (GRCm39)	V380E	probably benign	Het
Proc	T	A	18: 32,260,513 (GRCm39)	D204V	probably benign	Het
Prpsap2	C	T	11: 61,627,870 (GRCm39)	R202H	probably damaging	Het
Resf1	T	C	6: 149,236,179 (GRCm39)	F1500L	probably damaging	Het
Rtl1	T	G	12: 109,558,087 (GRCm39)	T1251P	possibly damaging	Het
Sema6a	T	G	18: 47,414,405 (GRCm39)	I482L	probably benign	Het
Smpdl3a	T	A	10: 57,681,654 (GRCm39)		probably null	Het
Strc	T	A	2: 121,209,790 (GRCm39)	M178L	probably benign	Het
Taok1	A	T	11: 77,451,144 (GRCm39)	M312K	probably damaging	Het
Tle2	T	C	10: 81,416,750 (GRCm39)		probably null	Het
Tle3	A	G	9: 61,316,190 (GRCm39)	D296G	probably benign	Het
Trank1	A	G	9: 111,191,485 (GRCm39)	D498G	probably benign	Het
Vars1	C	A	17: 35,231,491 (GRCm39)	L672M	probably damaging	Het
Vps13d	G	A	4: 144,826,580 (GRCm39)	T2866I	probably damaging	Het
Zfp423	C	A	8: 88,508,860 (GRCm39)	E370*	probably null	Het
Zfp521	T	A	18: 13,978,612 (GRCm39)	K600N	probably damaging	Het
Zfp788	A	G	7: 41,298,572 (GRCm39)	K351E	probably benign	Het
Zscan29	G	T	2: 120,994,518 (GRCm39)	T489N	probably damaging	Het

Other mutations in Zfp963

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0220:Zfp963	UTSW	8	70,196,145 (GRCm39)	missense	probably benign	0.09
R0220:Zfp963	UTSW	8	70,196,143 (GRCm39)	nonsense	probably null
R0423:Zfp963	UTSW	8	70,197,156 (GRCm39)	missense	probably damaging	1.00
R1171:Zfp963	UTSW	8	70,195,669 (GRCm39)	missense	possibly damaging	0.86
R1630:Zfp963	UTSW	8	70,196,837 (GRCm39)	splice site	probably benign
R1750:Zfp963	UTSW	8	70,196,100 (GRCm39)	missense	possibly damaging	0.81
R2090:Zfp963	UTSW	8	70,195,996 (GRCm39)	nonsense	probably null
R2185:Zfp963	UTSW	8	70,195,561 (GRCm39)	missense	probably benign	0.43
R4484:Zfp963	UTSW	8	70,197,135 (GRCm39)	missense	probably benign	0.29
R4600:Zfp963	UTSW	8	70,195,510 (GRCm39)	splice site	probably null
R4617:Zfp963	UTSW	8	70,195,944 (GRCm39)	missense	probably benign	0.00
R5427:Zfp963	UTSW	8	70,196,106 (GRCm39)	missense	probably benign	0.01
R5892:Zfp963	UTSW	8	70,196,027 (GRCm39)	missense	probably benign
R6073:Zfp963	UTSW	8	70,195,853 (GRCm39)	nonsense	probably null
R7312:Zfp963	UTSW	8	70,195,759 (GRCm39)	missense	probably damaging	1.00
R7739:Zfp963	UTSW	8	70,195,533 (GRCm39)	missense	possibly damaging	0.59
R9587:Zfp963	UTSW	8	70,195,692 (GRCm39)	missense	probably benign	0.24

Predicted Primers

PCR Primer
(F):5'- TGGAAGTGCAAGGTCTCCC -3'
(R):5'- GGACTAGCACCATATGAATCCCATA -3'

Sequencing Primer
(F):5'- AAAGGGTTTCTCTCCACCATGGG -3'
(R):5'- CTTTCAAAGGTGTACGGAAGCCC -3'

Posted On

2017-02-28