Mutagenetix > Incidental Mutation

Incidental Mutation 'R5917:Zfp423'

461400

Institutional Source

Beutler Lab

Gene Symbol

Zfp423

Ensembl Gene

ENSMUSG00000045333

Gene Name

zinc finger protein 423

Synonyms

Roaz, Zfp104, Ebfaz, ataxia1

MMRRC Submission

044114-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.832) question?

Stock #

R5917 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

88388438-88686223 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 88508860 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 370 (E370*)

Ref Sequence

ENSEMBL: ENSMUSP00000129724 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052250] [ENSMUST00000109655] [ENSMUST00000165770] [ENSMUST00000174249] [ENSMUST00000174764]

AlphaFold

Q80TS5

Predicted Effect

probably null
Transcript: ENSMUST00000052250
AA Change: E474*

SMART Domains

Protein: ENSMUSP00000052379
Gene: ENSMUSG00000045333
AA Change: E474*

Domain	Start	End	E-Value	Type
low complexity region	39	51	N/A	INTRINSIC
ZnF_C2H2	54	75	5.07e0	SMART
low complexity region	107	123	N/A	INTRINSIC
ZnF_C2H2	125	147	1.28e-3	SMART
ZnF_C2H2	153	175	1.64e-1	SMART
ZnF_C2H2	181	203	2.05e-2	SMART
ZnF_C2H2	209	231	3.21e-4	SMART
ZnF_C2H2	250	273	5.42e-2	SMART
ZnF_C2H2	282	305	1.76e-1	SMART
ZnF_C2H2	310	332	8.67e-1	SMART
low complexity region	350	364	N/A	INTRINSIC
ZnF_C2H2	396	420	1.16e-1	SMART
ZnF_C2H2	428	451	3.52e-1	SMART
ZnF_C2H2	467	490	7.9e-4	SMART
low complexity region	492	503	N/A	INTRINSIC
ZnF_C2H2	504	527	2.53e-2	SMART
ZnF_C2H2	550	575	3.99e0	SMART
low complexity region	591	602	N/A	INTRINSIC
ZnF_C2H2	619	641	3.16e-3	SMART
ZnF_C2H2	649	671	5.81e-2	SMART
ZnF_C2H2	679	702	4.87e-4	SMART
ZnF_C2H2	707	730	7.26e-3	SMART
ZnF_C2H2	737	760	4.79e-3	SMART
ZnF_C2H2	768	790	1.36e-2	SMART
ZnF_C2H2	794	817	4.72e-2	SMART
ZnF_C2H2	873	896	4.12e0	SMART
ZnF_C2H2	917	939	5.59e-4	SMART
ZnF_C2H2	946	968	6.42e-4	SMART
ZnF_C2H2	975	997	4.94e0	SMART
ZnF_C2H2	1007	1029	4.99e1	SMART
Pfam:zf-C2H2_6	1050	1068	1.6e-1	PFAM
ZnF_C2H2	1107	1130	1.12e-3	SMART
ZnF_C2H2	1155	1177	1.45e-2	SMART
ZnF_C2H2	1185	1207	5.72e-1	SMART
ZnF_C2H2	1216	1239	1.18e-2	SMART
ZnF_C2H2	1246	1269	4.05e-1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000109655
AA Change: E495*

SMART Domains

Protein: ENSMUSP00000105282
Gene: ENSMUSG00000045333
AA Change: E495*

Domain	Start	End	E-Value	Type
low complexity region	60	72	N/A	INTRINSIC
ZnF_C2H2	75	96	5.07e0	SMART
low complexity region	128	144	N/A	INTRINSIC
ZnF_C2H2	146	168	1.28e-3	SMART
ZnF_C2H2	174	196	1.64e-1	SMART
ZnF_C2H2	202	224	2.05e-2	SMART
ZnF_C2H2	230	252	3.21e-4	SMART
ZnF_C2H2	271	294	5.42e-2	SMART
ZnF_C2H2	303	326	1.76e-1	SMART
ZnF_C2H2	331	353	8.67e-1	SMART
low complexity region	371	385	N/A	INTRINSIC
ZnF_C2H2	417	441	1.16e-1	SMART
ZnF_C2H2	449	472	3.52e-1	SMART
ZnF_C2H2	488	511	7.9e-4	SMART
low complexity region	513	524	N/A	INTRINSIC
ZnF_C2H2	525	548	2.53e-2	SMART
ZnF_C2H2	571	596	3.99e0	SMART
low complexity region	612	623	N/A	INTRINSIC
ZnF_C2H2	640	662	3.16e-3	SMART
ZnF_C2H2	670	692	5.81e-2	SMART
ZnF_C2H2	700	723	4.87e-4	SMART
ZnF_C2H2	728	751	7.26e-3	SMART
ZnF_C2H2	758	781	4.79e-3	SMART
ZnF_C2H2	789	811	1.36e-2	SMART
ZnF_C2H2	815	838	4.72e-2	SMART
ZnF_C2H2	894	917	4.12e0	SMART
ZnF_C2H2	938	960	5.59e-4	SMART
ZnF_C2H2	967	989	6.42e-4	SMART
ZnF_C2H2	996	1018	4.94e0	SMART
ZnF_C2H2	1028	1050	4.99e1	SMART
ZnF_C2H2	1128	1151	1.12e-3	SMART
ZnF_C2H2	1176	1198	1.45e-2	SMART
ZnF_C2H2	1206	1228	5.72e-1	SMART
ZnF_C2H2	1237	1260	1.18e-2	SMART
ZnF_C2H2	1267	1290	4.05e-1	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000165770
AA Change: E370*

SMART Domains

Protein: ENSMUSP00000129724
Gene: ENSMUSG00000045333
AA Change: E370*

Domain	Start	End	E-Value	Type
low complexity region	3	19	N/A	INTRINSIC
ZnF_C2H2	21	43	1.28e-3	SMART
ZnF_C2H2	49	71	1.64e-1	SMART
ZnF_C2H2	77	99	2.05e-2	SMART
ZnF_C2H2	105	127	3.21e-4	SMART
ZnF_C2H2	146	169	5.42e-2	SMART
ZnF_C2H2	178	201	1.76e-1	SMART
ZnF_C2H2	206	228	8.67e-1	SMART
low complexity region	246	260	N/A	INTRINSIC
ZnF_C2H2	292	316	1.16e-1	SMART
ZnF_C2H2	324	347	3.52e-1	SMART
ZnF_C2H2	363	386	7.9e-4	SMART
low complexity region	388	399	N/A	INTRINSIC
ZnF_C2H2	400	423	2.53e-2	SMART
ZnF_C2H2	446	471	3.99e0	SMART
low complexity region	487	498	N/A	INTRINSIC
ZnF_C2H2	515	537	3.16e-3	SMART
ZnF_C2H2	545	567	5.81e-2	SMART
ZnF_C2H2	575	598	4.87e-4	SMART
ZnF_C2H2	603	626	7.26e-3	SMART
ZnF_C2H2	633	656	4.79e-3	SMART
ZnF_C2H2	664	686	1.36e-2	SMART
ZnF_C2H2	690	713	4.72e-2	SMART
ZnF_C2H2	769	792	4.12e0	SMART
ZnF_C2H2	813	835	5.59e-4	SMART
ZnF_C2H2	842	864	6.42e-4	SMART
ZnF_C2H2	871	893	4.94e0	SMART
ZnF_C2H2	903	925	4.99e1	SMART
Pfam:zf-C2H2_6	946	964	2.5e-1	PFAM
ZnF_C2H2	1003	1026	1.12e-3	SMART
ZnF_C2H2	1051	1073	1.45e-2	SMART
ZnF_C2H2	1081	1103	5.72e-1	SMART
ZnF_C2H2	1112	1135	1.18e-2	SMART
ZnF_C2H2	1142	1165	4.05e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173092

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173725

Predicted Effect

probably benign
Transcript: ENSMUST00000174249

SMART Domains

Protein: ENSMUSP00000134103
Gene: ENSMUSG00000045333

Domain	Start	End	E-Value	Type
low complexity region	60	76	N/A	INTRINSIC
ZnF_C2H2	78	100	1.28e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000174764

SMART Domains

Protein: ENSMUSP00000134575
Gene: ENSMUSG00000045333

Domain	Start	End	E-Value	Type
low complexity region	63	75	N/A	INTRINSIC
ZnF_C2H2	78	99	5.07e0	SMART
low complexity region	131	147	N/A	INTRINSIC
ZnF_C2H2	149	171	1.28e-3	SMART
ZnF_C2H2	177	199	1.64e-1	SMART
ZnF_C2H2	205	227	2.05e-2	SMART
Pfam:zf-C2H2_6	232	244	2.5e-1	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear protein that belongs to the family of Kruppel-like C2H2 zinc finger proteins. It functions as a DNA-binding transcription factor by using distinct zinc fingers in different signaling pathways. Thus, it is thought that this gene may have multiple roles in signal transduction during development. Mutations in this gene are associated with nephronophthisis-14 and Joubert syndrome-19. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2012]
PHENOTYPE: Mutations in this gene lead to postnatal lethality, abnormal gait, ataxia, reduced body size, loss of the corpus callosum, reduction of the hippocampus, olfactory bulb defects, and variable malformation of the cerebellum, including vermis agenesis, due to reduced proliferation of neural precursors. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	T	A	12: 118,832,516 (GRCm39)	R1153*	probably null	Het
Amdhd1	T	C	10: 93,360,332 (GRCm39)	H409R	possibly damaging	Het
Anks1b	C	T	10: 90,412,803 (GRCm39)		probably benign	Het
Ascc2	T	C	11: 4,631,506 (GRCm39)	L649P	probably benign	Het
Chst15	A	G	7: 131,872,246 (GRCm39)	F12L	probably benign	Het
Clec4a4	A	T	6: 122,981,017 (GRCm39)	K83N	probably benign	Het
Comp	T	A	8: 70,829,011 (GRCm39)		probably null	Het
Cryz	T	A	3: 154,327,403 (GRCm39)	S144T	probably benign	Het
Ctss	A	G	3: 95,450,424 (GRCm39)	D125G	probably benign	Het
Dact1	G	T	12: 71,365,456 (GRCm39)	V746L	possibly damaging	Het
Dhx29	A	G	13: 113,099,377 (GRCm39)	H1134R	probably damaging	Het
Dlgap4	A	G	2: 156,546,460 (GRCm39)	D376G	probably damaging	Het
Dnah3	T	A	7: 119,615,749 (GRCm39)	H1660L	probably damaging	Het
Ep300	T	A	15: 81,512,808 (GRCm39)		probably benign	Het
Fbxo30	A	T	10: 11,165,262 (GRCm39)		probably null	Het
Fcrl2	T	A	3: 87,164,094 (GRCm39)	H345L	probably damaging	Het
Galnt11	C	A	5: 25,452,670 (GRCm39)		probably null	Het
Il31ra	A	G	13: 112,682,846 (GRCm39)	C87R	probably benign	Het
Itga4	A	T	2: 79,117,442 (GRCm39)	Q416L	probably damaging	Het
Kcnt2	A	T	1: 140,461,666 (GRCm39)	T806S	probably damaging	Het
Lama2	A	G	10: 27,066,693 (GRCm39)	S1063P	probably damaging	Het
Lama4	T	A	10: 38,924,028 (GRCm39)	S479T	probably benign	Het
Lgi4	C	T	7: 30,759,603 (GRCm39)	T53M	possibly damaging	Het
Limk1	A	G	5: 134,686,789 (GRCm39)	F533L	probably damaging	Het
Loxl1	A	G	9: 58,220,006 (GRCm39)	L55P	probably damaging	Het
Map1a	A	G	2: 121,135,697 (GRCm39)	E1933G	probably damaging	Het
Matn2	T	A	15: 34,409,912 (GRCm39)	C447*	probably null	Het
Mmrn1	T	C	6: 60,950,134 (GRCm39)		probably null	Het
Or2a7	C	T	6: 43,151,646 (GRCm39)	S242F	probably damaging	Het
Or4a66	A	T	2: 88,531,049 (GRCm39)	I208N	possibly damaging	Het
Or4d1	A	T	11: 87,805,215 (GRCm39)	N172K	probably damaging	Het
Otor	A	G	2: 142,920,431 (GRCm39)	I4M	probably benign	Het
P2rx3	A	T	2: 84,865,591 (GRCm39)	V18E	probably damaging	Het
Pcdh7	T	C	5: 57,879,097 (GRCm39)	V884A	probably damaging	Het
Pcdhb4	T	A	18: 37,442,619 (GRCm39)	V643D	probably damaging	Het
Pelo	A	G	13: 115,225,930 (GRCm39)	S176P	possibly damaging	Het
Ppp2r3d	A	T	9: 101,089,183 (GRCm39)	V380E	probably benign	Het
Proc	T	A	18: 32,260,513 (GRCm39)	D204V	probably benign	Het
Prpsap2	C	T	11: 61,627,870 (GRCm39)	R202H	probably damaging	Het
Resf1	T	C	6: 149,236,179 (GRCm39)	F1500L	probably damaging	Het
Rtl1	T	G	12: 109,558,087 (GRCm39)	T1251P	possibly damaging	Het
Sema6a	T	G	18: 47,414,405 (GRCm39)	I482L	probably benign	Het
Smpdl3a	T	A	10: 57,681,654 (GRCm39)		probably null	Het
Strc	T	A	2: 121,209,790 (GRCm39)	M178L	probably benign	Het
Taok1	A	T	11: 77,451,144 (GRCm39)	M312K	probably damaging	Het
Tle2	T	C	10: 81,416,750 (GRCm39)		probably null	Het
Tle3	A	G	9: 61,316,190 (GRCm39)	D296G	probably benign	Het
Trank1	A	G	9: 111,191,485 (GRCm39)	D498G	probably benign	Het
Vars1	C	A	17: 35,231,491 (GRCm39)	L672M	probably damaging	Het
Vps13d	G	A	4: 144,826,580 (GRCm39)	T2866I	probably damaging	Het
Zfp521	T	A	18: 13,978,612 (GRCm39)	K600N	probably damaging	Het
Zfp788	A	G	7: 41,298,572 (GRCm39)	K351E	probably benign	Het
Zfp963	A	T	8: 70,195,510 (GRCm39)		probably null	Het
Zscan29	G	T	2: 120,994,518 (GRCm39)	T489N	probably damaging	Het

Other mutations in Zfp423

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01325:Zfp423	APN	8	88,508,239 (GRCm39)	splice site	probably null
IGL01359:Zfp423	APN	8	88,507,290 (GRCm39)	missense	probably damaging	1.00
IGL01780:Zfp423	APN	8	88,508,136 (GRCm39)	missense	probably damaging	1.00
IGL02250:Zfp423	APN	8	88,509,883 (GRCm39)	missense	probably damaging	1.00
IGL02301:Zfp423	APN	8	88,508,202 (GRCm39)	missense	probably damaging	1.00
IGL02320:Zfp423	APN	8	88,508,230 (GRCm39)	missense	probably damaging	1.00
IGL02804:Zfp423	APN	8	88,509,285 (GRCm39)	missense	probably benign	0.02
IGL03090:Zfp423	APN	8	88,508,071 (GRCm39)	missense	probably damaging	1.00
IGL03198:Zfp423	APN	8	88,508,304 (GRCm39)	missense	possibly damaging	0.73
IGL03383:Zfp423	APN	8	88,586,080 (GRCm39)	nonsense	probably null
swell	UTSW	8	88,413,187 (GRCm39)	splice site	probably null
Temptation	UTSW	8	88,508,381 (GRCm39)	missense	probably benign	0.25
trials	UTSW	8	88,507,341 (GRCm39)	missense	probably damaging	1.00
R0110:Zfp423	UTSW	8	88,508,887 (GRCm39)	missense	possibly damaging	0.60
R0142:Zfp423	UTSW	8	88,506,968 (GRCm39)	nonsense	probably null
R0256:Zfp423	UTSW	8	88,500,262 (GRCm39)	nonsense	probably null
R0538:Zfp423	UTSW	8	88,508,713 (GRCm39)	missense	probably damaging	0.99
R0542:Zfp423	UTSW	8	88,507,237 (GRCm39)	missense	probably damaging	1.00
R0614:Zfp423	UTSW	8	88,508,742 (GRCm39)	missense	probably damaging	1.00
R1179:Zfp423	UTSW	8	88,414,700 (GRCm39)	missense	probably damaging	0.97
R1417:Zfp423	UTSW	8	88,500,284 (GRCm39)	splice site	probably null
R1429:Zfp423	UTSW	8	88,413,070 (GRCm39)	missense	probably damaging	0.99
R1570:Zfp423	UTSW	8	88,509,186 (GRCm39)	missense	probably benign	0.37
R2013:Zfp423	UTSW	8	88,509,025 (GRCm39)	missense	probably benign	0.43
R2043:Zfp423	UTSW	8	88,509,246 (GRCm39)	missense	probably damaging	1.00
R2064:Zfp423	UTSW	8	88,507,986 (GRCm39)	missense	probably benign	0.04
R2108:Zfp423	UTSW	8	88,507,806 (GRCm39)	missense	possibly damaging	0.73
R2358:Zfp423	UTSW	8	88,507,179 (GRCm39)	missense	possibly damaging	0.56
R3177:Zfp423	UTSW	8	88,508,959 (GRCm39)	missense	probably damaging	1.00
R3277:Zfp423	UTSW	8	88,508,959 (GRCm39)	missense	probably damaging	1.00
R3738:Zfp423	UTSW	8	88,507,972 (GRCm39)	missense	probably damaging	1.00
R3739:Zfp423	UTSW	8	88,507,972 (GRCm39)	missense	probably damaging	1.00
R3773:Zfp423	UTSW	8	88,507,140 (GRCm39)	missense	probably benign	0.03
R4034:Zfp423	UTSW	8	88,507,972 (GRCm39)	missense	probably damaging	1.00
R4425:Zfp423	UTSW	8	88,509,601 (GRCm39)	missense	probably damaging	1.00
R4611:Zfp423	UTSW	8	88,414,709 (GRCm39)	missense	possibly damaging	0.90
R4700:Zfp423	UTSW	8	88,508,338 (GRCm39)	splice site	probably null
R4753:Zfp423	UTSW	8	88,508,074 (GRCm39)	missense	probably benign	0.00
R4818:Zfp423	UTSW	8	88,631,128 (GRCm39)	missense	probably benign	0.00
R5026:Zfp423	UTSW	8	88,507,302 (GRCm39)	missense	probably damaging	1.00
R5190:Zfp423	UTSW	8	88,509,091 (GRCm39)	missense	probably damaging	1.00
R5243:Zfp423	UTSW	8	88,500,275 (GRCm39)	missense	probably benign	0.03
R5284:Zfp423	UTSW	8	88,508,305 (GRCm39)	missense	possibly damaging	0.73
R5586:Zfp423	UTSW	8	88,585,968 (GRCm39)	missense	possibly damaging	0.93
R5601:Zfp423	UTSW	8	88,508,637 (GRCm39)	missense	probably damaging	1.00
R5671:Zfp423	UTSW	8	88,508,955 (GRCm39)	missense	probably damaging	0.99
R5717:Zfp423	UTSW	8	88,413,187 (GRCm39)	splice site	probably null
R5801:Zfp423	UTSW	8	88,585,990 (GRCm39)	missense	probably damaging	0.99
R5985:Zfp423	UTSW	8	88,508,774 (GRCm39)	missense	possibly damaging	0.83
R6111:Zfp423	UTSW	8	88,509,315 (GRCm39)	missense	probably damaging	0.99
R6306:Zfp423	UTSW	8	88,508,662 (GRCm39)	missense	possibly damaging	0.64
R6770:Zfp423	UTSW	8	88,508,445 (GRCm39)	missense	probably damaging	0.99
R6970:Zfp423	UTSW	8	88,530,407 (GRCm39)	missense	probably benign	0.00
R7029:Zfp423	UTSW	8	88,414,694 (GRCm39)	missense	probably damaging	0.99
R7060:Zfp423	UTSW	8	88,509,507 (GRCm39)	missense	probably damaging	1.00
R7074:Zfp423	UTSW	8	88,509,060 (GRCm39)	missense	probably benign	0.00
R7121:Zfp423	UTSW	8	88,507,489 (GRCm39)	missense	probably damaging	1.00
R7242:Zfp423	UTSW	8	88,631,155 (GRCm39)	missense	probably benign	0.07
R7359:Zfp423	UTSW	8	88,508,871 (GRCm39)	missense	possibly damaging	0.52
R7426:Zfp423	UTSW	8	88,507,341 (GRCm39)	missense	probably damaging	1.00
R7540:Zfp423	UTSW	8	88,414,695 (GRCm39)	missense	possibly damaging	0.95
R7640:Zfp423	UTSW	8	88,507,905 (GRCm39)	missense	probably damaging	1.00
R7767:Zfp423	UTSW	8	88,507,512 (GRCm39)	missense	probably damaging	1.00
R7938:Zfp423	UTSW	8	88,622,304 (GRCm39)	missense	unknown
R7986:Zfp423	UTSW	8	88,506,978 (GRCm39)	missense	probably benign	0.04
R8347:Zfp423	UTSW	8	88,509,784 (GRCm39)	missense	probably damaging	0.99
R8356:Zfp423	UTSW	8	88,509,910 (GRCm39)	missense	probably damaging	1.00
R8676:Zfp423	UTSW	8	88,509,338 (GRCm39)	missense	probably benign	0.04
R8710:Zfp423	UTSW	8	88,507,549 (GRCm39)	missense	possibly damaging	0.74
R8794:Zfp423	UTSW	8	88,507,857 (GRCm39)	missense	probably damaging	1.00
R8832:Zfp423	UTSW	8	88,507,827 (GRCm39)	missense	probably damaging	0.98
R9018:Zfp423	UTSW	8	88,508,381 (GRCm39)	missense	probably benign	0.25
R9182:Zfp423	UTSW	8	88,508,742 (GRCm39)	missense	probably damaging	0.99
R9309:Zfp423	UTSW	8	88,509,688 (GRCm39)	missense	probably damaging	0.99
R9312:Zfp423	UTSW	8	88,508,569 (GRCm39)	missense	probably damaging	1.00
R9453:Zfp423	UTSW	8	88,508,251 (GRCm39)	missense	probably damaging	1.00
R9469:Zfp423	UTSW	8	88,509,519 (GRCm39)	missense	probably damaging	0.99
R9480:Zfp423	UTSW	8	88,631,115 (GRCm39)	critical splice donor site	probably null
R9483:Zfp423	UTSW	8	88,507,725 (GRCm39)	missense	possibly damaging	0.90
R9510:Zfp423	UTSW	8	88,510,041 (GRCm39)	missense	possibly damaging	0.94
R9521:Zfp423	UTSW	8	88,509,033 (GRCm39)	missense	probably damaging	1.00
R9606:Zfp423	UTSW	8	88,414,595 (GRCm39)	missense	probably damaging	0.99
R9789:Zfp423	UTSW	8	88,506,877 (GRCm39)	missense	probably benign	0.03
Z1176:Zfp423	UTSW	8	88,586,048 (GRCm39)	missense	possibly damaging	0.49
Z1177:Zfp423	UTSW	8	88,507,553 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCTGAACAACAGGAGACTCCAG -3'
(R):5'- CCTGGCTGTGCTAGAGATTC -3'

Sequencing Primer
(F):5'- CAACAGGAGACTCCAGCTTGG -3'
(R):5'- CTGTGCTAGAGATTCATCTGAAGACC -3'

Posted On

2017-02-28