Mutagenetix > Incidental Mutation

Incidental Mutation 'R4510:Dclk3'

470175

Institutional Source

Beutler Lab

Gene Symbol

Dclk3

Ensembl Gene

ENSMUSG00000032500

Gene Name

doublecortin-like kinase 3

Synonyms

Dcamkl3, Click-I, -II related

MMRRC Submission

041585-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.188) question?

Stock #

R4510 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

111268149-111318186 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 111297060 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 201 (H201Q)

Ref Sequence

ENSEMBL: ENSMUSP00000107510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111879]

AlphaFold

Q8BWQ5

Predicted Effect

probably benign
Transcript: ENSMUST00000111879
AA Change: H201Q

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500
AA Change: H201Q

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
Pfam:DCX	115	177	2.9e-17	PFAM
low complexity region	200	218	N/A	INTRINSIC
low complexity region	267	277	N/A	INTRINSIC
low complexity region	479	496	N/A	INTRINSIC
S_TKc	514	771	1.63e-110	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.9%

Validation Efficiency

96% (50/52)

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9330159F19Rik	T	A	10: 29,100,819 (GRCm39)	D397E	probably benign	Het
Abcc10	A	G	17: 46,618,136 (GRCm39)	F1005L	probably damaging	Het
Ackr4	T	C	9: 103,975,930 (GRCm39)	E339G	probably benign	Het
Adam39	G	T	8: 41,279,328 (GRCm39)	C573F	probably damaging	Het
Anks1b	A	G	10: 90,346,652 (GRCm39)	T651A	probably benign	Het
Aoc1	C	A	6: 48,884,740 (GRCm39)	H594Q	probably damaging	Het
Arid1a	A	C	4: 133,423,010 (GRCm39)		probably benign	Het
Atp4a	G	T	7: 30,423,678 (GRCm39)	E928*	probably null	Het
Atp5pf	T	C	16: 84,624,862 (GRCm39)	D104G	probably benign	Het
Atp8b5	A	G	4: 43,320,629 (GRCm39)	T206A	probably damaging	Het
Atrn	G	A	2: 130,777,497 (GRCm39)	W182*	probably null	Het
Bltp2	T	C	11: 78,168,154 (GRCm39)	S1530P	possibly damaging	Het
Cacna1e	G	A	1: 154,437,579 (GRCm39)	T257I	probably damaging	Het
Caskin1	G	A	17: 24,725,602 (GRCm39)	S1296N	probably benign	Het
Crebrf	A	G	17: 26,961,938 (GRCm39)	Y345C	probably benign	Het
Cyp26b1	G	A	6: 84,551,473 (GRCm39)	R248W	probably damaging	Het
Ddx17	A	T	15: 79,422,793 (GRCm39)	V315E	probably damaging	Het
Dhrs11	T	C	11: 84,716,342 (GRCm39)	*51W	probably null	Het
Fgfr4	C	A	13: 55,309,328 (GRCm39)	P455Q	possibly damaging	Het
Gabbr1	A	G	17: 37,380,103 (GRCm39)	K697E	probably damaging	Het
Gcnt1	G	A	19: 17,307,641 (GRCm39)	T28I	probably benign	Het
Gga2	G	A	7: 121,620,301 (GRCm39)	T4M	unknown	Het
Gm12185	T	A	11: 48,799,305 (GRCm39)	H396L	possibly damaging	Het
Gm21976	A	G	13: 98,441,839 (GRCm39)	R123G	probably benign	Het
Hecw1	A	C	13: 14,531,776 (GRCm39)	V166G	probably damaging	Het
Hpx	A	G	7: 105,241,295 (GRCm39)	V372A	possibly damaging	Het
Igkv2-109	A	G	6: 68,279,962 (GRCm39)	Y61C	probably damaging	Het
Igsf1	A	G	X: 48,875,050 (GRCm39)	F789S	probably damaging	Het
Il4ra	A	G	7: 125,175,280 (GRCm39)	D496G	possibly damaging	Het
Ilf3	A	G	9: 21,310,511 (GRCm39)	T547A	possibly damaging	Het
Insrr	C	A	3: 87,715,978 (GRCm39)	P558T	possibly damaging	Het
Ints9	A	G	14: 65,266,381 (GRCm39)	D411G	possibly damaging	Het
Irak3	T	A	10: 119,981,813 (GRCm39)	H393L	probably damaging	Het
Isx	G	A	8: 75,600,298 (GRCm39)	M10I	probably benign	Het
Itga11	A	G	9: 62,668,870 (GRCm39)	D709G	probably damaging	Het
Kcng2	G	T	18: 80,338,930 (GRCm39)	R453S	probably benign	Het
Kif5b	A	G	18: 6,214,011 (GRCm39)	V664A	probably benign	Het
Lalba	A	G	15: 98,380,422 (GRCm39)	L44P	probably benign	Het
Ldlrad1	T	G	4: 107,066,715 (GRCm39)	F17V	probably benign	Het
Lnx1	C	T	5: 74,780,853 (GRCm39)	D382N	probably damaging	Het
Lrp1	T	C	10: 127,429,717 (GRCm39)	Y451C	probably damaging	Het
Lrp2	A	T	2: 69,310,406 (GRCm39)	N2722K	possibly damaging	Het
Mctp1	G	A	13: 76,973,391 (GRCm39)	V431I	probably benign	Het
Mmrn2	T	C	14: 34,125,016 (GRCm39)	F866L	possibly damaging	Het
Mylk2	A	G	2: 152,759,330 (GRCm39)	E367G	probably damaging	Het
Nfatc1	A	G	18: 80,678,794 (GRCm39)	S865P	probably damaging	Het
Nol6	G	C	4: 41,123,526 (GRCm39)	T74R	probably damaging	Het
Notch2	T	C	3: 98,053,637 (GRCm39)	M2100T	probably benign	Het
Parp1	A	G	1: 180,418,841 (GRCm39)	K667R	possibly damaging	Het
Phlda3	A	G	1: 135,694,400 (GRCm39)	T72A	probably damaging	Het
Polg	T	C	7: 79,105,270 (GRCm39)	Q758R	probably benign	Het
Polq	G	A	16: 36,868,925 (GRCm39)	R765H	probably damaging	Het
Pramel19	A	T	4: 101,798,757 (GRCm39)	M243L	probably benign	Het
Prkd1	T	C	12: 50,439,762 (GRCm39)	D355G	possibly damaging	Het
Prpf8	T	C	11: 75,382,652 (GRCm39)	Y398H	probably damaging	Het
Ripk1	T	C	13: 34,210,731 (GRCm39)	Y309H	probably damaging	Het
Rngtt	A	T	4: 33,339,032 (GRCm39)	Q279L	possibly damaging	Het
Rusf1	A	T	7: 127,875,312 (GRCm39)	F319Y	probably damaging	Het
Samd4	T	A	14: 47,315,042 (GRCm39)	V114D	probably benign	Het
Sec23ip	G	A	7: 128,380,900 (GRCm39)	E956K	probably damaging	Het
Slc4a1ap	A	T	5: 31,684,747 (GRCm39)	T128S	probably benign	Het
Slc5a11	A	T	7: 122,834,858 (GRCm39)	I6F	probably benign	Het
Slc6a19	A	C	13: 73,832,094 (GRCm39)	L494R	probably damaging	Het
Slc6a21	A	G	7: 44,936,713 (GRCm39)	D189G	probably damaging	Het
Slc7a1	G	T	5: 148,277,372 (GRCm39)	A381D	probably damaging	Het
Smc4	T	A	3: 68,923,980 (GRCm39)		probably null	Het
Stk19	T	C	17: 35,051,504 (GRCm39)	E17G	probably damaging	Het
Tekt5	A	C	16: 10,175,877 (GRCm39)	V556G	probably benign	Het
Tenm4	T	C	7: 96,544,070 (GRCm39)	F2029L	probably benign	Het
Thnsl1	T	C	2: 21,217,236 (GRCm39)	V330A	probably damaging	Het
Tnfrsf21	A	G	17: 43,375,910 (GRCm39)	D432G	probably damaging	Het
Tnip1	T	A	11: 54,817,616 (GRCm39)	S244C	probably benign	Het
Tnrc6c	A	T	11: 117,633,784 (GRCm39)	N1294I	possibly damaging	Het
Trpm3	A	G	19: 22,965,381 (GRCm39)	I1625M	probably benign	Het
Ttn	A	T	2: 76,575,773 (GRCm39)	V25040E	probably damaging	Het
Vwa5a	A	G	9: 38,633,853 (GRCm39)	N19D	possibly damaging	Het
Washc2	A	T	6: 116,197,517 (GRCm39)	D250V	probably damaging	Het
Xpo1	A	G	11: 23,237,401 (GRCm39)	T755A	possibly damaging	Het
Zfp462	C	T	4: 55,008,934 (GRCm39)	T300I	possibly damaging	Het
Zfp937	A	C	2: 150,080,431 (GRCm39)	T154P	probably damaging	Het
Zzef1	A	G	11: 72,778,996 (GRCm39)	D1819G	probably benign	Het

Other mutations in Dclk3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Dclk3	APN	9	111,296,911 (GRCm39)	nonsense	probably null
IGL02125:Dclk3	APN	9	111,298,175 (GRCm39)	missense	probably damaging	1.00
IGL02547:Dclk3	APN	9	111,298,091 (GRCm39)	missense	probably damaging	1.00
IGL03393:Dclk3	APN	9	111,317,741 (GRCm39)	utr 3 prime	probably benign
G1citation:Dclk3	UTSW	9	111,268,405 (GRCm39)	missense	probably benign	0.03
IGL02984:Dclk3	UTSW	9	111,317,643 (GRCm39)	missense	probably damaging	1.00
R0109:Dclk3	UTSW	9	111,296,738 (GRCm39)	missense	possibly damaging	0.93
R0109:Dclk3	UTSW	9	111,296,738 (GRCm39)	missense	possibly damaging	0.93
R0238:Dclk3	UTSW	9	111,311,696 (GRCm39)	missense	probably damaging	0.99
R0238:Dclk3	UTSW	9	111,311,696 (GRCm39)	missense	probably damaging	0.99
R0432:Dclk3	UTSW	9	111,314,003 (GRCm39)	missense	probably damaging	1.00
R0440:Dclk3	UTSW	9	111,298,231 (GRCm39)	missense	probably damaging	1.00
R0530:Dclk3	UTSW	9	111,311,789 (GRCm39)	missense	probably damaging	1.00
R1024:Dclk3	UTSW	9	111,298,138 (GRCm39)	missense	possibly damaging	0.95
R1443:Dclk3	UTSW	9	111,298,088 (GRCm39)	missense	probably benign	0.01
R1474:Dclk3	UTSW	9	111,298,304 (GRCm39)	missense	probably benign	0.43
R1479:Dclk3	UTSW	9	111,297,614 (GRCm39)	missense	probably benign
R1482:Dclk3	UTSW	9	111,296,888 (GRCm39)	missense	possibly damaging	0.90
R1543:Dclk3	UTSW	9	111,297,122 (GRCm39)	missense	probably benign	0.04
R1552:Dclk3	UTSW	9	111,317,647 (GRCm39)	missense	probably damaging	1.00
R1559:Dclk3	UTSW	9	111,298,276 (GRCm39)	missense	probably damaging	1.00
R2011:Dclk3	UTSW	9	111,297,422 (GRCm39)	missense	probably benign	0.00
R2369:Dclk3	UTSW	9	111,317,610 (GRCm39)	missense	probably benign	0.16
R4111:Dclk3	UTSW	9	111,298,148 (GRCm39)	missense	probably damaging	0.99
R4511:Dclk3	UTSW	9	111,297,060 (GRCm39)	missense	probably benign	0.01
R4592:Dclk3	UTSW	9	111,296,963 (GRCm39)	missense	probably damaging	1.00
R4604:Dclk3	UTSW	9	111,298,253 (GRCm39)	missense	probably damaging	1.00
R4857:Dclk3	UTSW	9	111,297,716 (GRCm39)	missense	probably benign
R4932:Dclk3	UTSW	9	111,297,110 (GRCm39)	missense	possibly damaging	0.56
R5045:Dclk3	UTSW	9	111,296,856 (GRCm39)	missense	probably damaging	0.99
R5233:Dclk3	UTSW	9	111,297,749 (GRCm39)	missense	probably benign
R5338:Dclk3	UTSW	9	111,298,127 (GRCm39)	missense	possibly damaging	0.95
R5463:Dclk3	UTSW	9	111,298,328 (GRCm39)	missense	probably benign	0.26
R6822:Dclk3	UTSW	9	111,268,405 (GRCm39)	missense	probably benign	0.03
R6995:Dclk3	UTSW	9	111,296,768 (GRCm39)	missense	possibly damaging	0.88
R7187:Dclk3	UTSW	9	111,314,064 (GRCm39)	missense	probably damaging	1.00
R7532:Dclk3	UTSW	9	111,296,596 (GRCm39)	missense	probably benign	0.03
R7534:Dclk3	UTSW	9	111,297,286 (GRCm39)	missense	probably benign
R7734:Dclk3	UTSW	9	111,298,163 (GRCm39)	missense	probably damaging	1.00
R8326:Dclk3	UTSW	9	111,296,602 (GRCm39)	missense	probably damaging	0.96
R8372:Dclk3	UTSW	9	111,314,081 (GRCm39)	missense	probably damaging	0.98
R8388:Dclk3	UTSW	9	111,311,813 (GRCm39)	missense	probably damaging	1.00
R8423:Dclk3	UTSW	9	111,297,787 (GRCm39)	missense	possibly damaging	0.93
R8493:Dclk3	UTSW	9	111,297,215 (GRCm39)	missense	probably benign	0.06
R9013:Dclk3	UTSW	9	111,297,566 (GRCm39)	missense	probably benign	0.31
R9114:Dclk3	UTSW	9	111,317,683 (GRCm39)	missense	probably benign	0.06
R9338:Dclk3	UTSW	9	111,268,373 (GRCm39)	missense	unknown
R9412:Dclk3	UTSW	9	111,311,819 (GRCm39)	critical splice donor site	probably null
R9701:Dclk3	UTSW	9	111,298,244 (GRCm39)	missense	probably damaging	1.00
R9776:Dclk3	UTSW	9	111,298,226 (GRCm39)	missense	probably damaging	1.00
R9802:Dclk3	UTSW	9	111,298,244 (GRCm39)	missense	probably damaging	1.00
X0020:Dclk3	UTSW	9	111,314,143 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

Posted On

2017-03-07