Mutagenetix > Incidental Mutation

Incidental Mutation 'R5968:Zdhhc5'

470687

Institutional Source

Beutler Lab

Gene Symbol

Zdhhc5

Ensembl Gene

ENSMUSG00000034075

Gene Name

zinc finger, DHHC domain containing 5

Synonyms

1110032A17Rik, Zisp

MMRRC Submission

043249-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.329) question?

Stock #

R5968 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

84518314-84545524 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 84524719 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000048198 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035840]

AlphaFold

Q8VDZ4

Predicted Effect

probably null
Transcript: ENSMUST00000035840

SMART Domains

Protein: ENSMUSP00000048198
Gene: ENSMUSG00000034075

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
transmembrane domain	45	67	N/A	INTRINSIC
Pfam:zf-DHHC	99	224	1.6e-37	PFAM
low complexity region	312	318	N/A	INTRINSIC
low complexity region	359	373	N/A	INTRINSIC
low complexity region	422	432	N/A	INTRINSIC
low complexity region	581	597	N/A	INTRINSIC
low complexity region	679	695	N/A	INTRINSIC
low complexity region	698	708	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 93.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	T	C	17: 46,621,077 (GRCm39)	T978A	probably benign	Het
Adcy9	A	G	16: 4,116,606 (GRCm39)	L638P	probably damaging	Het
Adgrf2	A	G	17: 43,026,063 (GRCm39)		probably null	Het
Anxa6	T	C	11: 54,885,167 (GRCm39)	I461V	probably damaging	Het
Arap1	T	C	7: 101,043,945 (GRCm39)	L668P	probably damaging	Het
Ces2e	G	T	8: 105,659,627 (GRCm39)	G498W	probably damaging	Het
Crb1	A	G	1: 139,170,739 (GRCm39)	C823R	probably damaging	Het
Ehmt1	C	T	2: 24,726,469 (GRCm39)	R772H	probably damaging	Het
Enpep	A	G	3: 129,074,587 (GRCm39)	L721S	probably benign	Het
Flii	T	G	11: 60,611,038 (GRCm39)	I464L	probably benign	Het
Gm57858	T	C	3: 36,064,840 (GRCm39)	Q511R	probably benign	Het
Gtf2h3	C	T	5: 124,722,360 (GRCm39)	T121I	probably benign	Het
Ift172	T	C	5: 31,418,828 (GRCm39)	E1162G	probably damaging	Het
Meioc	A	G	11: 102,566,657 (GRCm39)	S758G	probably damaging	Het
Ndst1	A	C	18: 60,846,148 (GRCm39)	S54A	probably benign	Het
Ndufaf8	G	T	11: 119,990,055 (GRCm39)	E56*	probably null	Het
Ndufb7	A	G	8: 84,293,530 (GRCm39)	D28G	probably benign	Het
Or1e16	T	C	11: 73,286,018 (GRCm39)	M277V	possibly damaging	Het
Or2a25	A	G	6: 42,888,480 (GRCm39)	I8V	probably benign	Het
Prkg1	A	T	19: 30,570,324 (GRCm39)	F443I	probably damaging	Het
Pspc1	C	T	14: 57,001,693 (GRCm39)	R227H	probably benign	Het
Ptpn21	A	G	12: 98,677,149 (GRCm39)	Y120H	probably damaging	Het
Runx1t1	A	T	4: 13,841,890 (GRCm39)		probably null	Het
Ryr3	T	C	2: 112,477,394 (GRCm39)	D4449G	probably benign	Het
Sacs	C	A	14: 61,427,078 (GRCm39)	A159E	probably damaging	Het
Slc16a14	T	C	1: 84,890,226 (GRCm39)	I360V	possibly damaging	Het
Tcstv5	T	C	13: 120,411,618 (GRCm39)		probably benign	Het
Thop1	A	G	10: 80,911,393 (GRCm39)	D93G	probably benign	Het
Tmem92	A	C	11: 94,669,564 (GRCm39)	M85R	probably benign	Het
Ttn	T	C	2: 76,688,017 (GRCm39)		probably benign	Het
Zfp335	T	C	2: 164,734,314 (GRCm39)	H1291R	probably damaging	Het
Zfp957	C	T	14: 79,451,496 (GRCm39)	C101Y	probably damaging	Het

Other mutations in Zdhhc5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01669:Zdhhc5	APN	2	84,521,538 (GRCm39)	missense	probably damaging	0.99
IGL01795:Zdhhc5	APN	2	84,520,390 (GRCm39)	missense	probably benign
IGL01862:Zdhhc5	APN	2	84,520,836 (GRCm39)	missense	probably benign	0.01
PIT4449001:Zdhhc5	UTSW	2	84,520,571 (GRCm39)	missense	probably damaging	1.00
R0270:Zdhhc5	UTSW	2	84,520,459 (GRCm39)	missense	probably benign	0.06
R0419:Zdhhc5	UTSW	2	84,521,587 (GRCm39)	splice site	probably null
R0543:Zdhhc5	UTSW	2	84,522,824 (GRCm39)	unclassified	probably benign
R1171:Zdhhc5	UTSW	2	84,522,685 (GRCm39)	missense	probably benign	0.00
R1450:Zdhhc5	UTSW	2	84,532,733 (GRCm39)	missense	probably damaging	0.99
R1922:Zdhhc5	UTSW	2	84,523,771 (GRCm39)	missense	probably damaging	0.99
R2229:Zdhhc5	UTSW	2	84,520,557 (GRCm39)	missense	probably damaging	1.00
R4799:Zdhhc5	UTSW	2	84,523,775 (GRCm39)	missense	probably damaging	0.97
R5473:Zdhhc5	UTSW	2	84,520,810 (GRCm39)	missense	probably damaging	0.99
R6299:Zdhhc5	UTSW	2	84,520,825 (GRCm39)	missense	probably benign	0.06
R6550:Zdhhc5	UTSW	2	84,526,685 (GRCm39)	missense	probably benign	0.03
R7069:Zdhhc5	UTSW	2	84,545,355 (GRCm39)	start gained	probably benign
R7169:Zdhhc5	UTSW	2	84,532,675 (GRCm39)	critical splice donor site	probably null
R7383:Zdhhc5	UTSW	2	84,524,748 (GRCm39)	missense	probably benign	0.44
R8703:Zdhhc5	UTSW	2	84,520,596 (GRCm39)	missense	probably benign	0.06
R9647:Zdhhc5	UTSW	2	84,524,750 (GRCm39)	missense	probably benign	0.01
R9789:Zdhhc5	UTSW	2	84,524,662 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACGTAGGCCTACTTCATTCAC -3'
(R):5'- CACGTATCCTTGAATGCCTTTG -3'

Sequencing Primer
(F):5'- ACTTTCCCCACTACAGGTAATATC -3'
(R):5'- GCCTTTGGCTTATAGGATGGG -3'

Posted On

2017-03-31