Mutagenetix > Incidental Mutation

Incidental Mutation 'R5956:Acsm2'

471190

Institutional Source

Beutler Lab

Gene Symbol

Acsm2

Ensembl Gene

ENSMUSG00000030945

Gene Name

acyl-CoA synthetase medium-chain family member 2

Synonyms

MMRRC Submission

044144-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5956 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

119153563-119199913 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 119153704 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 5 (S5P)

Ref Sequence

ENSEMBL: ENSMUSP00000095690 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098084]

AlphaFold

Q8K0L3

Predicted Effect

unknown
Transcript: ENSMUST00000098084
AA Change: S5P

SMART Domains

Protein: ENSMUSP00000095690
Gene: ENSMUSG00000030945
AA Change: S5P

Domain	Start	End	E-Value	Type
Pfam:AMP-binding	82	495	9.7e-71	PFAM
Pfam:AMP-binding_C	503	583	5.8e-22	PFAM

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.5%

Validation Efficiency

98% (63/64)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	T	A	12: 71,203,893 (GRCm39)	I398K	possibly damaging	Het
Acad9	A	G	3: 36,129,323 (GRCm39)		probably benign	Het
Akp3	T	C	1: 87,054,667 (GRCm39)	I334T	probably damaging	Het
Amy1	C	T	3: 113,357,311 (GRCm39)	R176H	probably benign	Het
Ank2	A	T	3: 126,736,337 (GRCm39)		probably benign	Het
Cav1	C	A	6: 17,307,918 (GRCm39)	N23K	probably damaging	Het
Cep126	A	G	9: 8,112,120 (GRCm39)	V151A	probably benign	Het
Cnot1	A	G	8: 96,481,606 (GRCm39)		probably null	Het
Crim1	T	A	17: 78,623,146 (GRCm39)	V448E	probably damaging	Het
Csmd3	T	C	15: 48,655,278 (GRCm39)	E8G	possibly damaging	Het
Ddx31	A	T	2: 28,764,185 (GRCm39)	I464F	probably damaging	Het
Ddx54	A	G	5: 120,764,432 (GRCm39)		probably benign	Het
Dhx16	A	G	17: 36,193,762 (GRCm39)	E319G	probably damaging	Het
Efl1	A	G	7: 82,301,107 (GRCm39)	D37G	probably damaging	Het
Epha5	T	C	5: 84,298,228 (GRCm39)	R444G	probably damaging	Het
Exoc2	C	A	13: 31,004,606 (GRCm39)	C859F	probably benign	Het
Gbx2	T	C	1: 89,860,908 (GRCm39)		probably benign	Het
Gm12258	G	A	11: 58,750,285 (GRCm39)	A9T	probably benign	Het
Gpr141b	T	G	13: 19,913,300 (GRCm39)		noncoding transcript	Het
Grm4	A	G	17: 27,654,129 (GRCm39)	V607A	probably benign	Het
Gvin3	T	C	7: 106,200,677 (GRCm39)		noncoding transcript	Het
Ighv1-74	C	A	12: 115,766,455 (GRCm39)	W55L	probably damaging	Het
Irx4	C	A	13: 73,415,626 (GRCm39)	Y138*	probably null	Het
Itpr1	C	A	6: 108,482,988 (GRCm39)	T2352K	probably benign	Het
Kcnk3	T	G	5: 30,745,854 (GRCm39)	V65G	probably damaging	Het
Mctp2	T	C	7: 71,908,923 (GRCm39)	E130G	probably benign	Het
Mgat5	C	A	1: 127,310,676 (GRCm39)	R197S	probably benign	Het
Mpg	T	C	11: 32,177,951 (GRCm39)		probably null	Het
Muc5b	T	C	7: 141,417,910 (GRCm39)	C3619R	probably damaging	Het
Myo15b	G	A	11: 115,764,583 (GRCm39)	V1321I	probably benign	Het
Myo1b	T	C	1: 51,815,391 (GRCm39)	T658A	probably damaging	Het
Nomo1	T	C	7: 45,692,037 (GRCm39)	S154P	possibly damaging	Het
Nsd1	T	A	13: 55,411,217 (GRCm39)	F1423I	probably damaging	Het
Or1e16	AGCGGTCGTAGGC	AGC	11: 73,286,480 (GRCm39)		probably null	Het
Or9g3	A	G	2: 85,584,183 (GRCm39)		probably benign	Het
Osbpl6	A	G	2: 76,379,856 (GRCm39)	R149G	probably damaging	Het
Pacc1	A	G	1: 191,080,568 (GRCm39)	S263G	probably damaging	Het
Papola	T	A	12: 105,777,300 (GRCm39)	W281R	probably damaging	Het
Pole	C	T	5: 110,485,153 (GRCm39)		probably benign	Het
Rptn	A	G	3: 93,305,334 (GRCm39)	N889S	possibly damaging	Het
Scn10a	A	G	9: 119,460,626 (GRCm39)	S1084P	probably damaging	Het
Scnm1	T	C	3: 95,037,596 (GRCm39)	I157V	probably benign	Het
Sertad2	G	A	11: 20,597,884 (GRCm39)	G27S	probably benign	Het
Siglece	T	C	7: 43,308,760 (GRCm39)	T198A	probably damaging	Het
Sptb	T	A	12: 76,650,942 (GRCm39)	M1678L	probably benign	Het
Taf8	A	T	17: 47,809,467 (GRCm39)	M98K	probably damaging	Het
Tead2	T	A	7: 44,870,138 (GRCm39)		probably benign	Het
Trrap	C	T	5: 144,744,201 (GRCm39)		silent	Het
Ttn	G	T	2: 76,775,914 (GRCm39)	N1709K	probably damaging	Het
Ube3a	T	C	7: 58,926,768 (GRCm39)		probably benign	Het
Ube3c	T	C	5: 29,804,054 (GRCm39)		probably benign	Het
Unc80	T	C	1: 66,567,123 (GRCm39)	S910P	probably damaging	Het
Usp30	G	A	5: 114,257,682 (GRCm39)	R280Q	possibly damaging	Het
Vsx1	T	C	2: 150,530,457 (GRCm39)	S142G	possibly damaging	Het
Wdr95	T	G	5: 149,517,947 (GRCm39)	C360G	probably benign	Het
Zbtb4	A	G	11: 69,669,040 (GRCm39)	I588V	probably benign	Het
Zdhhc4	A	T	5: 143,310,604 (GRCm39)		probably benign	Het
Zfp568	C	A	7: 29,697,288 (GRCm39)	N69K	probably damaging	Het

Other mutations in Acsm2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00782:Acsm2	APN	7	119,172,391 (GRCm39)	missense	probably damaging	1.00
IGL00930:Acsm2	APN	7	119,191,533 (GRCm39)	missense	possibly damaging	0.91
IGL01472:Acsm2	APN	7	119,153,759 (GRCm39)	critical splice donor site	probably null
IGL01927:Acsm2	APN	7	119,177,435 (GRCm39)	missense	possibly damaging	0.75
IGL02550:Acsm2	APN	7	119,172,507 (GRCm39)	missense	probably damaging	1.00
IGL02551:Acsm2	APN	7	119,172,507 (GRCm39)	missense	probably damaging	1.00
IGL02818:Acsm2	APN	7	119,172,804 (GRCm39)	splice site	probably null
IGL03064:Acsm2	APN	7	119,174,864 (GRCm39)	missense	probably damaging	0.98
PIT4469001:Acsm2	UTSW	7	119,177,408 (GRCm39)	missense	possibly damaging	0.51
R0395:Acsm2	UTSW	7	119,174,969 (GRCm39)	missense	probably damaging	1.00
R0416:Acsm2	UTSW	7	119,162,779 (GRCm39)	missense	probably benign	0.00
R0783:Acsm2	UTSW	7	119,172,340 (GRCm39)	missense	probably damaging	1.00
R1252:Acsm2	UTSW	7	119,172,468 (GRCm39)	missense	probably benign	0.15
R1432:Acsm2	UTSW	7	119,172,798 (GRCm39)	missense	possibly damaging	0.83
R1494:Acsm2	UTSW	7	119,174,855 (GRCm39)	missense	probably damaging	1.00
R1495:Acsm2	UTSW	7	119,177,349 (GRCm39)	missense	probably damaging	1.00
R1642:Acsm2	UTSW	7	119,162,860 (GRCm39)	missense	probably damaging	1.00
R1702:Acsm2	UTSW	7	119,172,787 (GRCm39)	missense	possibly damaging	0.88
R2082:Acsm2	UTSW	7	119,179,857 (GRCm39)	missense	probably benign	0.00
R2420:Acsm2	UTSW	7	119,162,857 (GRCm39)	missense	probably damaging	1.00
R3612:Acsm2	UTSW	7	119,190,553 (GRCm39)	missense	probably damaging	0.97
R4396:Acsm2	UTSW	7	119,195,143 (GRCm39)	missense	probably damaging	1.00
R4433:Acsm2	UTSW	7	119,153,732 (GRCm39)	missense	unknown
R4568:Acsm2	UTSW	7	119,162,740 (GRCm39)	missense	probably benign	0.00
R4718:Acsm2	UTSW	7	119,172,826 (GRCm39)	missense	probably damaging	0.96
R5025:Acsm2	UTSW	7	119,153,719 (GRCm39)	missense	unknown
R5497:Acsm2	UTSW	7	119,172,543 (GRCm39)	missense	possibly damaging	0.69
R5509:Acsm2	UTSW	7	119,172,840 (GRCm39)	missense	probably damaging	1.00
R5682:Acsm2	UTSW	7	119,162,774 (GRCm39)	missense	probably benign	0.12
R5941:Acsm2	UTSW	7	119,190,321 (GRCm39)	missense	probably damaging	1.00
R6129:Acsm2	UTSW	7	119,190,470 (GRCm39)	splice site	probably null
R6212:Acsm2	UTSW	7	119,172,505 (GRCm39)	missense	probably damaging	1.00
R7026:Acsm2	UTSW	7	119,191,450 (GRCm39)	missense	probably damaging	1.00
R7227:Acsm2	UTSW	7	119,190,556 (GRCm39)	missense	probably benign
R7903:Acsm2	UTSW	7	119,195,215 (GRCm39)	missense	probably benign	0.22
R7954:Acsm2	UTSW	7	119,179,952 (GRCm39)	missense	probably damaging	1.00
R8002:Acsm2	UTSW	7	119,172,480 (GRCm39)	missense	possibly damaging	0.81
R8066:Acsm2	UTSW	7	119,190,548 (GRCm39)	missense	probably damaging	0.99
R9185:Acsm2	UTSW	7	119,177,421 (GRCm39)	missense	possibly damaging	0.96
R9200:Acsm2	UTSW	7	119,179,839 (GRCm39)	nonsense	probably null
R9324:Acsm2	UTSW	7	119,179,856 (GRCm39)	missense	probably benign
R9507:Acsm2	UTSW	7	119,179,939 (GRCm39)	missense	probably benign
R9623:Acsm2	UTSW	7	119,181,975 (GRCm39)	missense	probably benign	0.00
Z1177:Acsm2	UTSW	7	119,177,316 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCTGGACTACATTGTGTGGTGT -3'
(R):5'- AGCGAGTTGATGAATTGCAC -3'

Sequencing Primer
(F):5'- ACATTGTGTGGTGTATATAAAGGAC -3'
(R):5'- TGACATCAGTGTGGGACATCCTC -3'

Posted On

2017-03-31