Mutagenetix > Incidental Mutation

Incidental Mutation 'R5960:Ifi209'

471388

Institutional Source

Beutler Lab

Gene Symbol

Ifi209

Ensembl Gene

ENSMUSG00000043263

Gene Name

interferon activated gene 209

Synonyms

Ifix, Pyhin-1, Pyhin1

MMRRC Submission

044147-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.126) question?

Stock #

R5960 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

173458483-173475494 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 173466382 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000061900 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056071] [ENSMUST00000193727]

AlphaFold

Q8BV49

Predicted Effect

probably null
Transcript: ENSMUST00000056071

SMART Domains

Protein: ENSMUSP00000061900
Gene: ENSMUSG00000043263

Domain	Start	End	E-Value	Type
PYRIN	6	83	3.54e-17	SMART
low complexity region	152	169	N/A	INTRINSIC
Pfam:HIN	231	396	4.2e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000193727

SMART Domains

Protein: ENSMUSP00000142161
Gene: ENSMUSG00000043263

Domain	Start	End	E-Value	Type
PYRIN	6	83	1.7e-21	SMART
low complexity region	152	169	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200598

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.8%
20x: 92.9%

Validation Efficiency

99% (71/72)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579F01Rik	T	C	3: 137,889,528 (GRCm39)	T30A	possibly damaging	Het
Adat1	T	C	8: 112,709,233 (GRCm39)	M197V	probably benign	Het
Alcam	T	C	16: 52,115,489 (GRCm39)	T210A	probably benign	Het
Ankfy1	T	A	11: 72,648,178 (GRCm39)	S886R	possibly damaging	Het
Aopep	G	A	13: 63,388,087 (GRCm39)	R22Q	probably damaging	Het
Atg2a	T	G	19: 6,304,390 (GRCm39)	F1136V	probably damaging	Het
Birc6	A	T	17: 74,835,760 (GRCm39)	T72S	probably damaging	Het
Caskin1	A	G	17: 24,717,869 (GRCm39)	T219A	probably benign	Het
Catsperg1	A	G	7: 28,884,208 (GRCm39)		probably benign	Het
Cdh12	A	G	15: 21,492,562 (GRCm39)		probably null	Het
Cfap126	A	G	1: 170,952,882 (GRCm39)	D45G	probably damaging	Het
Ciz1	C	A	2: 32,261,228 (GRCm39)	Q356K	possibly damaging	Het
Ckmt1	T	A	2: 121,194,058 (GRCm39)	I407N	probably damaging	Het
Csmd1	C	T	8: 16,121,430 (GRCm39)	E1756K	possibly damaging	Het
Cwf19l2	A	G	9: 3,411,404 (GRCm39)	K94E	probably benign	Het
Cyth1	A	G	11: 118,023,193 (GRCm39)		probably benign	Het
Ddx25	A	G	9: 35,465,807 (GRCm39)		probably null	Het
Dnah2	C	T	11: 69,349,746 (GRCm39)	R2399Q	probably benign	Het
Dock3	C	A	9: 106,788,554 (GRCm39)	G138*	probably null	Het
Fam20a	G	A	11: 109,566,795 (GRCm39)		probably benign	Het
Fanci	A	G	7: 79,093,510 (GRCm39)	T1006A	probably damaging	Het
Fat1	T	C	8: 45,486,405 (GRCm39)	Y3320H	probably damaging	Het
Fndc8	A	G	11: 82,788,398 (GRCm39)	D76G	probably benign	Het
Gm19965	T	C	1: 116,749,201 (GRCm39)	I294T	possibly damaging	Het
Gm9755	T	G	8: 67,967,840 (GRCm39)		noncoding transcript	Het
Iars1	T	C	13: 49,878,113 (GRCm39)	V879A	possibly damaging	Het
Itgb2l	T	C	16: 96,227,459 (GRCm39)	H528R	probably benign	Het
Marf1	T	C	16: 13,970,281 (GRCm39)	Q146R	probably damaging	Het
Megf11	A	G	9: 64,567,731 (GRCm39)	T407A	probably benign	Het
Mtg1	T	C	7: 139,726,906 (GRCm39)		probably benign	Het
Nhsl1	T	G	10: 18,402,724 (GRCm39)	S1317A	probably benign	Het
Nhsl3	A	G	4: 129,115,865 (GRCm39)	V933A	probably damaging	Het
Nudt16	A	T	9: 105,008,698 (GRCm39)	C63S	possibly damaging	Het
Nup107	T	C	10: 117,625,915 (GRCm39)	I49V	probably null	Het
Or51a24	G	T	7: 103,733,560 (GRCm39)	N242K	probably damaging	Het
Or5m13	T	A	2: 85,749,069 (GRCm39)	S267T	probably benign	Het
Orc1	T	C	4: 108,463,495 (GRCm39)	S671P	possibly damaging	Het
Paox	A	G	7: 139,712,402 (GRCm39)	D211G	probably benign	Het
Pck2	A	G	14: 55,786,004 (GRCm39)	T571A	possibly damaging	Het
Pigg	A	G	5: 108,484,160 (GRCm39)	E469G	probably benign	Het
Pikfyve	C	A	1: 65,292,597 (GRCm39)	Y1349*	probably null	Het
Prickle2	A	G	6: 92,353,286 (GRCm39)	F783L	probably benign	Het
Prpf40b	G	T	15: 99,212,785 (GRCm39)	R627L	probably damaging	Het
Rasgrf1	A	G	9: 89,903,437 (GRCm39)	I1217V	possibly damaging	Het
Rbm26	A	T	14: 105,387,751 (GRCm39)	V457D	probably damaging	Het
Rex1bd	C	A	8: 70,959,156 (GRCm39)	R49L	probably null	Het
Robo2	A	T	16: 73,730,603 (GRCm39)	L1003Q	probably damaging	Het
Sacs	A	G	14: 61,446,144 (GRCm39)	D2730G	probably benign	Het
Setd6	T	A	8: 96,442,827 (GRCm39)	L88H	probably damaging	Het
Smim35	T	C	9: 45,154,288 (GRCm39)	Y50H	probably damaging	Het
Sncb	A	G	13: 54,910,795 (GRCm39)		probably benign	Het
Spata31e2	T	A	1: 26,722,225 (GRCm39)	H985L	probably benign	Het
Spmap2	T	G	10: 79,421,765 (GRCm39)	K151T	possibly damaging	Het
Stard9	A	G	2: 120,530,442 (GRCm39)	E2233G	probably benign	Het
Susd2	C	T	10: 75,475,770 (GRCm39)	V410I	probably damaging	Het
Synm	A	G	7: 67,385,494 (GRCm39)	S281P	probably damaging	Het
Syvn1	C	T	19: 6,100,598 (GRCm39)	R330C	probably damaging	Het
Tiam2	A	T	17: 3,488,915 (GRCm39)	D741V	probably benign	Het
Tph1	A	G	7: 46,311,429 (GRCm39)		probably null	Het
Trav7-5	T	A	14: 53,768,706 (GRCm39)	H91Q	probably benign	Het
Tymp	A	T	15: 89,260,778 (GRCm39)		probably null	Het
Usp45	A	C	4: 21,810,797 (GRCm39)	D331A	probably damaging	Het

Other mutations in Ifi209

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00422:Ifi209	APN	1	173,466,529 (GRCm39)	missense	possibly damaging	0.88
IGL02598:Ifi209	APN	1	173,472,281 (GRCm39)	missense	probably damaging	0.99
IGL02712:Ifi209	APN	1	173,470,267 (GRCm39)	missense	possibly damaging	0.68
IGL03131:Ifi209	APN	1	173,468,800 (GRCm39)	missense	possibly damaging	0.86
IGL03368:Ifi209	APN	1	173,470,057 (GRCm39)	missense	possibly damaging	0.91
R0880:Ifi209	UTSW	1	173,472,379 (GRCm39)	missense	probably damaging	1.00
R1317:Ifi209	UTSW	1	173,465,029 (GRCm39)	missense	possibly damaging	0.96
R1640:Ifi209	UTSW	1	173,464,931 (GRCm39)	missense	probably damaging	0.98
R1769:Ifi209	UTSW	1	173,468,728 (GRCm39)	missense	probably benign	0.11
R2349:Ifi209	UTSW	1	173,470,122 (GRCm39)	missense	probably damaging	1.00
R5096:Ifi209	UTSW	1	173,472,300 (GRCm39)	missense	probably benign	0.17
R5369:Ifi209	UTSW	1	173,464,873 (GRCm39)	start codon destroyed	probably null	1.00
R5484:Ifi209	UTSW	1	173,468,640 (GRCm39)	missense	probably benign	0.18
R5532:Ifi209	UTSW	1	173,466,542 (GRCm39)	missense	probably damaging	0.99
R5551:Ifi209	UTSW	1	173,468,763 (GRCm39)	missense	probably benign	0.01
R5554:Ifi209	UTSW	1	173,468,763 (GRCm39)	missense	probably benign	0.01
R5749:Ifi209	UTSW	1	173,464,893 (GRCm39)	missense	probably damaging	1.00
R6401:Ifi209	UTSW	1	173,472,269 (GRCm39)	missense	probably damaging	0.99
R7042:Ifi209	UTSW	1	173,470,236 (GRCm39)	missense	probably benign	0.34
R7304:Ifi209	UTSW	1	173,470,156 (GRCm39)	missense	possibly damaging	0.88
R7521:Ifi209	UTSW	1	173,470,261 (GRCm39)	missense	probably damaging	0.97
R7742:Ifi209	UTSW	1	173,470,198 (GRCm39)	missense	probably damaging	1.00
R7763:Ifi209	UTSW	1	173,470,445 (GRCm39)	missense	probably damaging	1.00
R7975:Ifi209	UTSW	1	173,468,722 (GRCm39)	missense	probably benign	0.24
R8498:Ifi209	UTSW	1	173,470,069 (GRCm39)	missense	probably benign	0.05
R8873:Ifi209	UTSW	1	173,470,156 (GRCm39)	missense	probably damaging	1.00
R9178:Ifi209	UTSW	1	173,464,969 (GRCm39)	missense	probably damaging	1.00
R9673:Ifi209	UTSW	1	173,470,332 (GRCm39)	missense	probably damaging	1.00
R9752:Ifi209	UTSW	1	173,472,235 (GRCm39)	missense	probably damaging	0.99
Z1088:Ifi209	UTSW	1	173,468,712 (GRCm39)	missense	probably benign	0.00
Z1088:Ifi209	UTSW	1	173,464,973 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CTGATAGACATAGAAGTCCCCAG -3'
(R):5'- TTCCACTGGGATCTTAGCCAC -3'

Sequencing Primer
(F):5'- TAGACATAGAAGTCCCCAGAATGG -3'
(R):5'- GGGATCTTAGCCACCATACCTGTG -3'

Posted On

2017-03-31