Incidental Mutation 'R5976:Zfp541'
ID 471763
Institutional Source Beutler Lab
Gene Symbol Zfp541
Ensembl Gene ENSMUSG00000078796
Gene Name zinc finger protein 541
Synonyms EG666528
MMRRC Submission 044158-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.750) question?
Stock # R5976 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 15795739-15830259 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 15810344 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Lysine to Arginine at position 127 (K127R)
Ref Sequence ENSEMBL: ENSMUSP00000148143 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000108509] [ENSMUST00000209369] [ENSMUST00000210805]
AlphaFold Q0GGX2
Predicted Effect probably benign
Transcript: ENSMUST00000108509
AA Change: K127R

PolyPhen 2 Score 0.344 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000104149
Gene: ENSMUSG00000078796
AA Change: K127R

DomainStartEndE-ValueType
low complexity region 48 65 N/A INTRINSIC
ZnF_C2H2 140 162 2.24e-3 SMART
ZnF_C2H2 168 190 4.47e-3 SMART
ZnF_C2H2 196 221 2.36e-2 SMART
low complexity region 249 260 N/A INTRINSIC
low complexity region 362 374 N/A INTRINSIC
low complexity region 444 465 N/A INTRINSIC
low complexity region 473 484 N/A INTRINSIC
low complexity region 594 609 N/A INTRINSIC
low complexity region 659 670 N/A INTRINSIC
ZnF_C2H2 838 860 3.72e0 SMART
low complexity region 953 958 N/A INTRINSIC
ELM2 1065 1122 4.46e-14 SMART
SANT 1171 1219 8.38e-7 SMART
low complexity region 1252 1263 N/A INTRINSIC
ZnF_C2H2 1301 1323 1.01e-1 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000209369
AA Change: K127R

PolyPhen 2 Score 0.344 (Sensitivity: 0.90; Specificity: 0.89)
Predicted Effect probably benign
Transcript: ENSMUST00000210805
AA Change: K127R

PolyPhen 2 Score 0.344 (Sensitivity: 0.90; Specificity: 0.89)
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.0%
  • 20x: 94.2%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ankar T C 1: 72,682,450 (GRCm39) T1154A probably benign Het
Ankrd26 A T 6: 118,494,855 (GRCm39) probably null Het
Arih2 A T 9: 108,485,172 (GRCm39) *54R probably null Het
AW146154 T G 7: 41,129,721 (GRCm39) K465T probably damaging Het
Bend3 A G 10: 43,386,540 (GRCm39) Y311C probably benign Het
Bltp2 G A 11: 78,174,955 (GRCm39) A1697T probably benign Het
Ccdc110 A T 8: 46,396,536 (GRCm39) Y809F possibly damaging Het
Ccnh C T 13: 85,338,982 (GRCm39) P76L probably damaging Het
Chaf1a T A 17: 56,371,115 (GRCm39) C667S probably damaging Het
Clca3a1 A T 3: 144,452,636 (GRCm39) Y616N probably damaging Het
Cldn4 A G 5: 134,975,410 (GRCm39) C64R probably damaging Het
Crybg2 TGGAGGAGGAGGAGGAGGAG TGGAGGAGGAGGAGGAG 4: 133,801,837 (GRCm39) probably benign Het
Dclk2 C A 3: 86,694,532 (GRCm39) R752L possibly damaging Het
Dzank1 C A 2: 144,343,409 (GRCm39) G318W probably damaging Het
Edem1 T A 6: 108,819,923 (GRCm39) I236K probably damaging Het
Eif4e1b T C 13: 54,932,635 (GRCm39) F75L probably damaging Het
Elmo3 A G 8: 106,034,279 (GRCm39) Y266C probably damaging Het
Enpep A G 3: 129,092,773 (GRCm39) S509P probably damaging Het
Exoc8 A G 8: 125,623,392 (GRCm39) M325T probably benign Het
Fah A G 7: 84,243,949 (GRCm39) M270T probably benign Het
Gabbr1 T C 17: 37,378,754 (GRCm39) L532P probably damaging Het
Gm10770 T A 2: 150,021,320 (GRCm39) K66* probably null Het
Gprc5c A T 11: 114,755,313 (GRCm39) Q330L possibly damaging Het
Grin3a T A 4: 49,792,602 (GRCm39) H377L probably damaging Het
Hipk3 T C 2: 104,301,529 (GRCm39) E221G probably damaging Het
Hsd17b6 T A 10: 127,827,308 (GRCm39) M255L probably benign Het
Ighv1-7 T A 12: 114,502,379 (GRCm39) E29D probably benign Het
Ing3 T A 6: 21,971,173 (GRCm39) S326T probably benign Het
Ipo7 T C 7: 109,648,014 (GRCm39) L632P probably damaging Het
Kdm6b A G 11: 69,294,614 (GRCm39) probably null Het
Kif21a T G 15: 90,820,015 (GRCm39) D1583A probably damaging Het
Lama2 C T 10: 27,066,672 (GRCm39) V1070I probably benign Het
Lrp1 A T 10: 127,419,770 (GRCm39) S946R probably damaging Het
Lrrc37a A G 11: 103,389,897 (GRCm39) S1843P possibly damaging Het
Ltbp1 T C 17: 75,597,078 (GRCm39) Y517H probably damaging Het
Map2k4 T A 11: 65,600,778 (GRCm39) N51I probably benign Het
Mfsd2b G T 12: 4,916,522 (GRCm39) A216D probably damaging Het
Nbea T C 3: 55,761,268 (GRCm39) T2025A probably benign Het
Neb A G 2: 52,106,928 (GRCm39) V4162A possibly damaging Het
Nr3c1 A T 18: 39,554,602 (GRCm39) F599I probably damaging Het
Nsun2 T G 13: 69,771,271 (GRCm39) probably null Het
Or10ab4 T A 7: 107,655,005 (GRCm39) M272K possibly damaging Het
Or8b37 G T 9: 37,958,997 (GRCm39) V160F possibly damaging Het
Otoa T A 7: 120,726,936 (GRCm39) W524R probably benign Het
Paip1 T A 13: 119,593,533 (GRCm39) D182E probably damaging Het
Pde1a G A 2: 79,698,586 (GRCm39) Q415* probably null Het
Pfkfb2 T A 1: 130,635,816 (GRCm39) K72* probably null Het
Pigg A G 5: 108,480,057 (GRCm39) E444G probably null Het
Plec T C 15: 76,073,237 (GRCm39) Y669C probably damaging Het
Ptp4a3 T C 15: 73,627,885 (GRCm39) V94A possibly damaging Het
Ptprg A G 14: 12,211,625 (GRCm38) E969G probably damaging Het
R3hcc1l T A 19: 42,551,789 (GRCm39) V262E probably benign Het
Ranbp3l T G 15: 9,030,916 (GRCm39) F65C possibly damaging Het
Rbm19 T A 5: 120,278,372 (GRCm39) S718R probably benign Het
Recql4 C A 15: 76,593,624 (GRCm39) R162L probably benign Het
Rest T C 5: 77,416,119 (GRCm39) L111P probably benign Het
Rgma T C 7: 73,059,216 (GRCm39) S13P probably damaging Het
Rogdi T A 16: 4,831,175 (GRCm39) I31F probably benign Het
Serpinb9e T A 13: 33,439,112 (GRCm39) D179E probably benign Het
Slc1a5 T C 7: 16,529,807 (GRCm39) C409R probably damaging Het
Slc25a38 A G 9: 119,945,613 (GRCm39) T38A probably damaging Het
Spag17 C T 3: 100,003,107 (GRCm39) Q1897* probably null Het
St7 C A 6: 17,694,221 (GRCm39) A4E possibly damaging Het
Tbcel T A 9: 42,350,499 (GRCm39) I263F possibly damaging Het
Tmtc3 A G 10: 100,312,534 (GRCm39) V103A probably benign Het
Tnc G A 4: 63,936,403 (GRCm39) P178S probably benign Het
Vwf A G 6: 125,580,426 (GRCm39) D558G Het
Other mutations in Zfp541
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02162:Zfp541 APN 7 15,813,393 (GRCm39) missense possibly damaging 0.80
IGL02262:Zfp541 APN 7 15,813,620 (GRCm39) missense probably damaging 1.00
IGL02347:Zfp541 APN 7 15,817,390 (GRCm39) missense probably damaging 1.00
IGL02516:Zfp541 APN 7 15,816,918 (GRCm39) splice site probably null
R0101:Zfp541 UTSW 7 15,811,968 (GRCm39) missense probably damaging 1.00
R0412:Zfp541 UTSW 7 15,816,099 (GRCm39) missense possibly damaging 0.88
R0627:Zfp541 UTSW 7 15,829,607 (GRCm39) splice site probably benign
R0784:Zfp541 UTSW 7 15,816,917 (GRCm39) intron probably benign
R1083:Zfp541 UTSW 7 15,812,637 (GRCm39) missense probably benign 0.16
R1541:Zfp541 UTSW 7 15,812,437 (GRCm39) missense probably benign 0.04
R1575:Zfp541 UTSW 7 15,812,640 (GRCm39) missense possibly damaging 0.94
R1730:Zfp541 UTSW 7 15,811,898 (GRCm39) missense probably damaging 0.99
R1783:Zfp541 UTSW 7 15,811,898 (GRCm39) missense probably damaging 0.99
R1966:Zfp541 UTSW 7 15,812,996 (GRCm39) missense probably benign 0.02
R2022:Zfp541 UTSW 7 15,816,110 (GRCm39) missense probably damaging 1.00
R2048:Zfp541 UTSW 7 15,812,252 (GRCm39) missense possibly damaging 0.94
R2436:Zfp541 UTSW 7 15,810,373 (GRCm39) missense possibly damaging 0.94
R3973:Zfp541 UTSW 7 15,806,147 (GRCm39) missense probably damaging 1.00
R4081:Zfp541 UTSW 7 15,806,060 (GRCm39) missense probably benign 0.16
R4589:Zfp541 UTSW 7 15,817,261 (GRCm39) missense probably benign 0.35
R4724:Zfp541 UTSW 7 15,815,612 (GRCm39) missense probably damaging 0.99
R4812:Zfp541 UTSW 7 15,813,035 (GRCm39) missense probably benign 0.01
R4817:Zfp541 UTSW 7 15,824,307 (GRCm39) missense probably damaging 1.00
R5232:Zfp541 UTSW 7 15,829,104 (GRCm39) missense probably damaging 1.00
R5331:Zfp541 UTSW 7 15,829,683 (GRCm39) missense probably damaging 1.00
R5551:Zfp541 UTSW 7 15,824,786 (GRCm39) missense probably damaging 1.00
R6259:Zfp541 UTSW 7 15,829,451 (GRCm39) missense probably benign 0.02
R6523:Zfp541 UTSW 7 15,829,445 (GRCm39) missense probably damaging 1.00
R6826:Zfp541 UTSW 7 15,812,907 (GRCm39) missense probably damaging 0.96
R7319:Zfp541 UTSW 7 15,813,294 (GRCm39) missense probably benign 0.21
R7428:Zfp541 UTSW 7 15,826,793 (GRCm39) missense probably damaging 1.00
R7594:Zfp541 UTSW 7 15,810,311 (GRCm39) missense probably damaging 0.96
R7724:Zfp541 UTSW 7 15,805,919 (GRCm39) missense probably damaging 1.00
R8276:Zfp541 UTSW 7 15,813,009 (GRCm39) missense possibly damaging 0.87
R8433:Zfp541 UTSW 7 15,805,999 (GRCm39) missense probably benign 0.16
R9068:Zfp541 UTSW 7 15,812,376 (GRCm39) missense probably damaging 0.96
R9086:Zfp541 UTSW 7 15,824,329 (GRCm39) missense probably damaging 1.00
R9132:Zfp541 UTSW 7 15,816,966 (GRCm39) missense probably benign 0.45
R9261:Zfp541 UTSW 7 15,816,029 (GRCm39) missense possibly damaging 0.94
R9408:Zfp541 UTSW 7 15,806,139 (GRCm39) missense probably damaging 1.00
R9518:Zfp541 UTSW 7 15,813,036 (GRCm39) missense possibly damaging 0.80
R9621:Zfp541 UTSW 7 15,805,892 (GRCm39) missense possibly damaging 0.94
Z1088:Zfp541 UTSW 7 15,813,720 (GRCm39) missense probably benign 0.16
Z1176:Zfp541 UTSW 7 15,812,191 (GRCm39) missense probably damaging 1.00
Z1177:Zfp541 UTSW 7 15,816,092 (GRCm39) missense probably damaging 0.98
Z1177:Zfp541 UTSW 7 15,812,954 (GRCm39) missense probably benign 0.26
Z1177:Zfp541 UTSW 7 15,812,688 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CTCAGCTAAGCCTTGTCGTCTG -3'
(R):5'- AGATTCTTGACATACAGGTGGTCC -3'

Sequencing Primer
(F):5'- GTGGGGGAGTCTCAGACTAC -3'
(R):5'- TGGTCCTGCCGCTTGAAG -3'
Posted On 2017-03-31