Mutagenetix > Incidental Mutation

Incidental Mutation 'R5963:Gpr87'

471863

Institutional Source

Beutler Lab

Gene Symbol

Gpr87

Ensembl Gene

ENSMUSG00000051431

Gene Name

G protein-coupled receptor 87

Synonyms

MMRRC Submission

044148-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5963 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

59086344-59102525 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 59086690 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 272 (R272*)

Ref Sequence

ENSEMBL: ENSMUSP00000143683 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040325] [ENSMUST00000056898] [ENSMUST00000164225] [ENSMUST00000199659] [ENSMUST00000200095]

AlphaFold

Q99MT7

Predicted Effect

probably benign
Transcript: ENSMUST00000040325

SMART Domains

Protein: ENSMUSP00000042269
Gene: ENSMUSG00000056476

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	730	2.6e-207	PFAM
low complexity region	744	758	N/A	INTRINSIC
low complexity region	853	872	N/A	INTRINSIC
low complexity region	1455	1466	N/A	INTRINSIC
low complexity region	1728	1742	N/A	INTRINSIC
low complexity region	1769	1783	N/A	INTRINSIC
Pfam:Med12-PQL	1803	2029	2.3e-14	PFAM
low complexity region	2055	2076	N/A	INTRINSIC
low complexity region	2083	2101	N/A	INTRINSIC
low complexity region	2116	2136	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000056898
AA Change: R273*

SMART Domains

Protein: ENSMUSP00000059272
Gene: ENSMUSG00000051431
AA Change: R273*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	60	315	1.4e-36	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000164225

SMART Domains

Protein: ENSMUSP00000127038
Gene: ENSMUSG00000056476

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	283	765	5e-187	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1763	1777	N/A	INTRINSIC
low complexity region	1804	1818	N/A	INTRINSIC
Pfam:Med12-PQL	1840	2063	9.7e-66	PFAM
low complexity region	2090	2111	N/A	INTRINSIC
low complexity region	2118	2136	N/A	INTRINSIC
low complexity region	2151	2171	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000199659

SMART Domains

Protein: ENSMUSP00000142903
Gene: ENSMUSG00000056476

Domain	Start	End	E-Value	Type
Med12	101	161	1.71e-24	SMART
low complexity region	216	224	N/A	INTRINSIC
low complexity region	269	278	N/A	INTRINSIC
Pfam:Med12-LCEWAV	282	765	5.5e-209	PFAM
low complexity region	779	793	N/A	INTRINSIC
low complexity region	888	907	N/A	INTRINSIC
low complexity region	1490	1501	N/A	INTRINSIC
low complexity region	1761	1775	N/A	INTRINSIC
low complexity region	1802	1816	N/A	INTRINSIC
Pfam:Med12-PQL	1836	2062	1.7e-15	PFAM
low complexity region	2088	2130	N/A	INTRINSIC
low complexity region	2144	2164	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199833

Predicted Effect

probably null
Transcript: ENSMUST00000200095
AA Change: R272*

SMART Domains

Protein: ENSMUSP00000143683
Gene: ENSMUSG00000051431
AA Change: R272*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	59	314	3.5e-37	PFAM

Meta Mutation Damage Score

0.9718

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 92.5%

Validation Efficiency

99% (68/69)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a G protein-coupled receptor and is located in a cluster of G protein-couple receptor genes on chromosome 3. The encoded protein has been shown to be overexpressed in lung squamous cell carcinoma (PMID:18057535) and regulated by p53 (PMID:19602589). [provided by RefSeq, Nov 2011]
PHENOTYPE: Mice homozygous for a null allele exhibit decreased mean vertebral and femoral bone measurements. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alg8	A	G	7: 97,029,037 (GRCm39)	K131E	probably benign	Het
Ank3	T	A	10: 69,823,056 (GRCm39)	L575*	probably null	Het
Aqr	T	C	2: 113,957,442 (GRCm39)	T819A	probably damaging	Het
Bbs2	A	T	8: 94,807,659 (GRCm39)	S407T	probably benign	Het
Bmper	T	A	9: 23,286,889 (GRCm39)	C272S	probably benign	Het
Btbd9	C	T	17: 30,553,192 (GRCm39)		probably null	Het
C5ar1	A	G	7: 15,982,747 (GRCm39)	V91A	possibly damaging	Het
Ccdc138	A	G	10: 58,411,579 (GRCm39)	H649R	possibly damaging	Het
Cfap43	A	T	19: 47,734,013 (GRCm39)	V1451E	probably benign	Het
Cfap46	T	A	7: 139,231,511 (GRCm39)	M901L	probably damaging	Het
Cherp	G	T	8: 73,215,379 (GRCm39)		probably benign	Het
D430041D05Rik	C	T	2: 104,078,630 (GRCm39)	V1229I	possibly damaging	Het
Fat4	A	C	3: 39,064,696 (GRCm39)	D4884A	probably damaging	Het
Fcrl5	T	C	3: 87,351,480 (GRCm39)	F243L	probably damaging	Het
Garem1	T	A	18: 21,262,487 (GRCm39)	I776F	probably benign	Het
Gsdmc	A	T	15: 63,651,965 (GRCm39)		probably null	Het
Hydin	T	A	8: 111,220,926 (GRCm39)	F1441I	possibly damaging	Het
Ints11	T	A	4: 155,957,369 (GRCm39)	C63*	probably null	Het
Kdm4b	A	T	17: 56,706,732 (GRCm39)	T908S	probably damaging	Het
Lipn	A	G	19: 34,058,700 (GRCm39)	D304G	probably damaging	Het
Man2a1	T	G	17: 64,982,117 (GRCm39)	N544K	probably benign	Het
Mapkapk5	G	T	5: 121,676,544 (GRCm39)	H66N	probably damaging	Het
Mog	G	A	17: 37,323,240 (GRCm39)	R233*	probably null	Het
Mrps24	A	T	11: 5,657,481 (GRCm39)		probably benign	Het
Nom1	T	C	5: 29,642,768 (GRCm39)	L423P	probably damaging	Het
Or51b6	T	A	7: 103,556,168 (GRCm39)	V174E	probably benign	Het
Pcdha1	T	A	18: 37,064,224 (GRCm39)	V296E	probably damaging	Het
Pdcd2	T	C	17: 15,746,656 (GRCm39)	K168E	possibly damaging	Het
Pdcd2	G	T	17: 15,746,657 (GRCm39)	H167Q	probably damaging	Het
Pla2g12b	T	A	10: 59,239,780 (GRCm39)	V63D	probably damaging	Het
Pnn	C	T	12: 59,114,617 (GRCm39)	R56*	probably null	Het
Primpol	T	A	8: 47,046,615 (GRCm39)	E227V	possibly damaging	Het
Rad54l	A	T	4: 115,967,584 (GRCm39)	W233R	probably damaging	Het
Rims2	A	T	15: 39,300,578 (GRCm39)	D103V	probably damaging	Het
Rttn	T	C	18: 89,091,819 (GRCm39)	S1511P	probably benign	Het
Septin5	T	C	16: 18,442,962 (GRCm39)		probably null	Het
Simc1	T	A	13: 54,673,632 (GRCm39)	I660K	possibly damaging	Het
Slc15a5	A	G	6: 138,056,691 (GRCm39)	L75P	probably damaging	Het
Slc9a2	A	G	1: 40,721,196 (GRCm39)	S55G	possibly damaging	Het
Slit3	C	T	11: 35,591,063 (GRCm39)	R1292C	probably damaging	Het
Slitrk3	A	G	3: 72,958,046 (GRCm39)	V242A	probably benign	Het
St14	A	T	9: 31,017,853 (GRCm39)		probably benign	Het
Tnnt2	T	C	1: 135,771,600 (GRCm39)		probably benign	Het
Traf5	G	A	1: 191,731,977 (GRCm39)	T288I	probably benign	Het
Trerf1	C	T	17: 47,625,263 (GRCm39)		noncoding transcript	Het
Vegfc	A	G	8: 54,634,319 (GRCm39)	N333D	probably benign	Het
Vmn1r65	T	A	7: 6,011,608 (GRCm39)	I209F	probably damaging	Het
Vps51	A	G	19: 6,118,320 (GRCm39)	L725P	probably damaging	Het
Vps8	T	C	16: 21,288,871 (GRCm39)	I408T	possibly damaging	Het
Wdr72	T	C	9: 74,052,310 (GRCm39)	Y114H	probably damaging	Het
Ybx2	A	G	11: 69,831,918 (GRCm39)	E164G	probably damaging	Het
Zcchc14	A	T	8: 122,355,362 (GRCm39)		probably benign	Het
Zfp383	A	G	7: 29,615,103 (GRCm39)	T453A	possibly damaging	Het
Zfp84	G	T	7: 29,476,378 (GRCm39)	G357C	probably damaging	Het
Zmynd11	G	T	13: 9,745,931 (GRCm39)		probably benign	Het

Other mutations in Gpr87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00496:Gpr87	APN	3	59,087,211 (GRCm39)	missense	probably damaging	1.00
IGL02309:Gpr87	APN	3	59,086,975 (GRCm39)	missense	possibly damaging	0.74
IGL02614:Gpr87	APN	3	59,086,738 (GRCm39)	missense	probably damaging	1.00
R0097:Gpr87	UTSW	3	59,086,506 (GRCm39)	missense	probably damaging	1.00
R0097:Gpr87	UTSW	3	59,086,506 (GRCm39)	missense	probably damaging	1.00
R0113:Gpr87	UTSW	3	59,086,932 (GRCm39)	missense	possibly damaging	0.86
R1801:Gpr87	UTSW	3	59,086,813 (GRCm39)	missense	possibly damaging	0.92
R2213:Gpr87	UTSW	3	59,086,465 (GRCm39)	missense	probably damaging	0.99
R2698:Gpr87	UTSW	3	59,086,587 (GRCm39)	missense	probably damaging	1.00
R5459:Gpr87	UTSW	3	59,087,148 (GRCm39)	missense	possibly damaging	0.89
R5490:Gpr87	UTSW	3	59,086,747 (GRCm39)	missense	probably damaging	0.99
R6189:Gpr87	UTSW	3	59,086,650 (GRCm39)	missense	probably damaging	1.00
R6251:Gpr87	UTSW	3	59,086,528 (GRCm39)	missense	probably damaging	0.99
R7863:Gpr87	UTSW	3	59,087,317 (GRCm39)	missense	probably damaging	1.00
R8008:Gpr87	UTSW	3	59,087,466 (GRCm39)	missense	probably benign
R8065:Gpr87	UTSW	3	59,087,308 (GRCm39)	missense	probably damaging	1.00
R8067:Gpr87	UTSW	3	59,087,308 (GRCm39)	missense	probably damaging	1.00
R8096:Gpr87	UTSW	3	59,087,229 (GRCm39)	missense	possibly damaging	0.70
R8141:Gpr87	UTSW	3	59,087,020 (GRCm39)	missense	probably damaging	1.00
R8326:Gpr87	UTSW	3	59,102,395 (GRCm39)	start gained	probably benign
R9326:Gpr87	UTSW	3	59,086,609 (GRCm39)	missense	probably damaging	1.00
R9485:Gpr87	UTSW	3	59,087,005 (GRCm39)	missense	possibly damaging	0.85
Z1177:Gpr87	UTSW	3	59,087,491 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GATCTTCGGACGCTTTGCAG -3'
(R):5'- TGTCACCTATGTGGACAGCTG -3'

Sequencing Primer
(F):5'- AGCGACCTGATGCTTTCG -3'
(R):5'- CACCTATGTGGACAGCTGTTTGTTTG -3'

Posted On

2017-03-31