Mutagenetix > Incidental Mutation

Incidental Mutation 'R0504:Piezo2'

47414

Institutional Source

Beutler Lab

Gene Symbol

Piezo2

Ensembl Gene

ENSMUSG00000041482

Gene Name

piezo-type mechanosensitive ion channel component 2

Synonyms

Piezo2, 9030411M15Rik, Fam38b2, Fam38b, 9430028L06Rik

MMRRC Submission

038699-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0504 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

63143284-63520254 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 63157522 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 2396 (T2396I)

Ref Sequence

ENSEMBL: ENSMUSP00000040019 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047480]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000047480
AA Change: T2396I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000040019
Gene: ENSMUSG00000041482
AA Change: T2396I

Domain	Start	End	E-Value	Type
transmembrane domain	13	44	N/A	INTRINSIC
transmembrane domain	59	81	N/A	INTRINSIC
low complexity region	179	194	N/A	INTRINSIC
transmembrane domain	214	236	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	267	289	N/A	INTRINSIC
coiled coil region	455	482	N/A	INTRINSIC
transmembrane domain	504	526	N/A	INTRINSIC
transmembrane domain	539	561	N/A	INTRINSIC
SCOP:d1eq1a_	597	666	4e-3	SMART
transmembrane domain	682	704	N/A	INTRINSIC
transmembrane domain	708	730	N/A	INTRINSIC
internal_repeat_1	740	764	6.01e-5	PROSPERO
low complexity region	772	784	N/A	INTRINSIC
transmembrane domain	791	813	N/A	INTRINSIC
low complexity region	900	921	N/A	INTRINSIC
transmembrane domain	949	971	N/A	INTRINSIC
transmembrane domain	976	993	N/A	INTRINSIC
transmembrane domain	1000	1022	N/A	INTRINSIC
transmembrane domain	1069	1091	N/A	INTRINSIC
transmembrane domain	1130	1152	N/A	INTRINSIC
transmembrane domain	1156	1173	N/A	INTRINSIC
transmembrane domain	1186	1208	N/A	INTRINSIC
transmembrane domain	1234	1256	N/A	INTRINSIC
transmembrane domain	1308	1327	N/A	INTRINSIC
transmembrane domain	1331	1353	N/A	INTRINSIC
Pfam:PIEZO	1383	1617	1.1e-105	PFAM
low complexity region	1807	1823	N/A	INTRINSIC
low complexity region	1836	1860	N/A	INTRINSIC
low complexity region	1863	1878	N/A	INTRINSIC
transmembrane domain	1981	2003	N/A	INTRINSIC
transmembrane domain	2010	2027	N/A	INTRINSIC
internal_repeat_1	2036	2060	6.01e-5	PROSPERO
low complexity region	2167	2199	N/A	INTRINSIC
transmembrane domain	2261	2283	N/A	INTRINSIC
transmembrane domain	2303	2325	N/A	INTRINSIC
transmembrane domain	2332	2354	N/A	INTRINSIC
transmembrane domain	2364	2386	N/A	INTRINSIC
Pfam:Piezo_RRas_bdg	2412	2821	2.8e-161	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123322

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132576

Predicted Effect

unknown
Transcript: ENSMUST00000137141
AA Change: T218I

SMART Domains

Protein: ENSMUSP00000117107
Gene: ENSMUSG00000041482
AA Change: T218I

Domain	Start	End	E-Value	Type
low complexity region	3	22	N/A	INTRINSIC
transmembrane domain	84	106	N/A	INTRINSIC
transmembrane domain	126	148	N/A	INTRINSIC
transmembrane domain	155	177	N/A	INTRINSIC
transmembrane domain	187	209	N/A	INTRINSIC
Pfam:Piezo_RRas_bdg	235	409	4.6e-78	PFAM

Meta Mutation Damage Score

0.4000

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.6%

Validation Efficiency

99% (145/147)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene contains more than thirty transmembrane domains and likely functions as part of mechanically-activated (MA) cation channels. These channels serve to connect mechanical forces to biological signals. The encoded protein quickly adapts MA currents in somatosensory neurons. Defects in this gene are a cause of type 5 distal arthrogryposis. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]

Allele List at MGI

Other mutations in this stock

Total: 145 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921539E11Rik	A	G	4: 103,128,057 (GRCm39)		probably benign	Het
Adamts6	T	C	13: 104,563,438 (GRCm39)		probably benign	Het
Adamts9	T	A	6: 92,889,626 (GRCm39)	Y316F	probably damaging	Het
Agl	A	T	3: 116,580,433 (GRCm39)	F374I	probably damaging	Het
Akr1c19	A	G	13: 4,286,250 (GRCm39)	T83A	possibly damaging	Het
Ankrd36	T	A	11: 5,579,274 (GRCm39)	S179R	probably damaging	Het
Appbp2	A	G	11: 85,082,513 (GRCm39)	S573P	probably benign	Het
Arid4a	T	A	12: 71,093,988 (GRCm39)	F254I	probably damaging	Het
Bin3	T	C	14: 70,361,336 (GRCm39)		probably null	Het
Bmi1	T	C	2: 18,688,883 (GRCm39)		probably null	Het
Bmper	G	T	9: 23,317,983 (GRCm39)	C534F	probably damaging	Het
Bora	T	A	14: 99,299,059 (GRCm39)	C205*	probably null	Het
Btnl2	A	G	17: 34,577,091 (GRCm39)	E82G	probably benign	Het
Ccdc8	A	T	7: 16,729,939 (GRCm39)	D476V	unknown	Het
Ccr3	C	A	9: 123,829,478 (GRCm39)	T271K	possibly damaging	Het
Cd276	A	G	9: 58,447,961 (GRCm39)	L23P	possibly damaging	Het
Cd3e	T	C	9: 44,913,552 (GRCm39)	Q61R	probably benign	Het
Cep97	A	G	16: 55,726,142 (GRCm39)	S582P	probably benign	Het
Chml	A	T	1: 175,514,748 (GRCm39)	M391K	probably damaging	Het
Chst1	A	G	2: 92,444,169 (GRCm39)	N214D	probably benign	Het
Chuk	T	C	19: 44,070,377 (GRCm39)		probably benign	Het
Col12a1	G	A	9: 79,588,750 (GRCm39)	H1122Y	possibly damaging	Het
Cpne6	A	G	14: 55,752,059 (GRCm39)	K272R	probably damaging	Het
Cpsf2	T	A	12: 101,956,262 (GRCm39)	L355Q	probably damaging	Het
Cyp2c29	T	A	19: 39,298,224 (GRCm39)	D256E	probably benign	Het
Daglb	G	A	5: 143,479,952 (GRCm39)	V420I	probably benign	Het
Ddx42	G	T	11: 106,138,675 (GRCm39)	G825C	probably benign	Het
Dis3	T	C	14: 99,318,826 (GRCm39)		probably benign	Het
Dkk4	C	T	8: 23,115,359 (GRCm39)	R70C	probably damaging	Het
Dock6	G	T	9: 21,713,732 (GRCm39)	Q1933K	probably damaging	Het
Dpep2	T	G	8: 106,716,620 (GRCm39)	Q186H	probably benign	Het
Dzip3	A	C	16: 48,780,006 (GRCm39)		probably benign	Het
Egflam	T	A	15: 7,252,239 (GRCm39)	I853F	probably damaging	Het
Fastkd5	A	G	2: 130,457,837 (GRCm39)	I251T	probably benign	Het
Fbn2	T	A	18: 58,172,532 (GRCm39)	D2091V	possibly damaging	Het
Fem1al	T	C	11: 29,774,990 (GRCm39)	I156V	probably damaging	Het
Fer1l4	C	A	2: 155,894,115 (GRCm39)	V63L	probably benign	Het
Frem1	T	A	4: 82,830,874 (GRCm39)	D2062V	probably benign	Het
Galnt6	A	C	15: 100,594,538 (GRCm39)		probably benign	Het
Get3	A	C	8: 85,745,236 (GRCm39)	V277G	probably damaging	Het
Gm10972	A	T	3: 94,550,440 (GRCm39)		probably benign	Het
Gm4846	G	A	1: 166,319,114 (GRCm39)	T208I	probably benign	Het
Gorab	A	G	1: 163,214,174 (GRCm39)	L252P	probably damaging	Het
Gtsf2	A	G	15: 103,352,988 (GRCm39)	C63R	probably damaging	Het
Hal	T	C	10: 93,325,036 (GRCm39)	V15A	probably damaging	Het
Hmcn1	A	T	1: 150,752,170 (GRCm39)		probably benign	Het
Hormad2	A	T	11: 4,358,833 (GRCm39)	H191Q	possibly damaging	Het
Hspa2	A	T	12: 76,451,990 (GRCm39)	D228V	probably damaging	Het
Igfn1	A	T	1: 135,896,267 (GRCm39)	M1433K	probably benign	Het
Il18	A	G	9: 50,486,628 (GRCm39)	D19G	probably damaging	Het
Il1rl2	G	A	1: 40,368,216 (GRCm39)	V129I	probably benign	Het
Inpp5b	C	A	4: 124,676,201 (GRCm39)	Y352*	probably null	Het
Insrr	A	T	3: 87,720,463 (GRCm39)	M1034L	possibly damaging	Het
Jmjd1c	T	C	10: 67,061,534 (GRCm39)	S1296P	probably damaging	Het
Kdm5b	G	T	1: 134,548,761 (GRCm39)		probably null	Het
Krba1	C	T	6: 48,393,188 (GRCm39)	T998I	probably benign	Het
L3mbtl4	A	G	17: 69,084,907 (GRCm39)	N606S	probably benign	Het
Lonrf1	T	A	8: 36,698,313 (GRCm39)	N395I	possibly damaging	Het
Lpp	A	G	16: 24,790,720 (GRCm39)	D393G	probably damaging	Het
Lrrc17	A	G	5: 21,765,528 (GRCm39)	I3M	probably benign	Het
Lrrtm4	A	T	6: 79,999,029 (GRCm39)	Q147L	probably damaging	Het
Map1a	A	G	2: 121,133,422 (GRCm39)	M1413V	probably benign	Het
Mapk8ip2	A	G	15: 89,340,861 (GRCm39)	E102G	possibly damaging	Het
Marf1	C	T	16: 13,960,398 (GRCm39)	A549T	probably damaging	Het
Mdn1	T	C	4: 32,698,916 (GRCm39)		probably benign	Het
Mfng	A	C	15: 78,641,514 (GRCm39)	H294Q	probably benign	Het
Mical2	T	A	7: 111,870,524 (GRCm39)	N4K	probably benign	Het
Mov10l1	T	A	15: 88,883,042 (GRCm39)	V384E	probably damaging	Het
Myo18b	A	G	5: 113,021,442 (GRCm39)		probably benign	Het
Nlrp1b	T	G	11: 71,073,241 (GRCm39)	I201L	probably damaging	Het
Nos2	C	T	11: 78,830,903 (GRCm39)	P249L	probably damaging	Het
Notch4	A	G	17: 34,794,065 (GRCm39)	T681A	probably damaging	Het
Nr1i3	C	T	1: 171,044,805 (GRCm39)		probably benign	Het
Obscn	A	G	11: 58,899,333 (GRCm39)		probably null	Het
Onecut2	A	T	18: 64,473,820 (GRCm39)	I124F	possibly damaging	Het
Or10a49	C	T	7: 108,468,057 (GRCm39)	M101I	possibly damaging	Het
Or2a14	A	T	6: 43,130,395 (GRCm39)	H52L	probably benign	Het
Or2ag1	T	A	7: 106,313,908 (GRCm39)		probably benign	Het
Or2f2	C	T	6: 42,767,530 (GRCm39)	R186*	probably null	Het
Or2t47	C	A	11: 58,442,462 (GRCm39)	C201F	probably damaging	Het
Or4a75	A	T	2: 89,448,438 (GRCm39)	Y33N	probably damaging	Het
Or4c123	C	T	2: 89,127,083 (GRCm39)	C177Y	probably damaging	Het
Or52u1	C	T	7: 104,237,682 (GRCm39)	R224*	probably null	Het
Or5m12	T	A	2: 85,735,030 (GRCm39)	M123L	possibly damaging	Het
Or8b35	A	G	9: 37,904,438 (GRCm39)	T217A	probably benign	Het
Otol1	G	A	3: 69,934,937 (GRCm39)	G310R	probably damaging	Het
Oxct2b	T	A	4: 123,010,633 (GRCm39)	S184R	possibly damaging	Het
Oxct2b	ACTG	A	4: 123,010,705 (GRCm39)		probably benign	Het
P2rx6	A	G	16: 17,385,291 (GRCm39)		probably benign	Het
Pde4a	A	G	9: 21,115,699 (GRCm39)	N411S	probably damaging	Het
Phkb	A	T	8: 86,783,153 (GRCm39)	D983V	probably benign	Het
Pik3ap1	T	A	19: 41,275,929 (GRCm39)	D717V	probably damaging	Het
Plce1	T	A	19: 38,766,465 (GRCm39)		probably benign	Het
Plekhg1	A	G	10: 3,887,853 (GRCm39)	I261V	probably damaging	Het
Ppfia4	T	C	1: 134,251,851 (GRCm39)	H441R	probably damaging	Het
Prpf8	T	A	11: 75,392,768 (GRCm39)		probably benign	Het
Ptn	T	A	6: 36,718,388 (GRCm39)		probably benign	Het
Ptpn13	A	G	5: 103,649,362 (GRCm39)	Y255C	possibly damaging	Het
Ptpn4	A	G	1: 119,693,645 (GRCm39)	Y126H	probably damaging	Het
Ptprc	T	C	1: 138,016,435 (GRCm39)	N505D	probably damaging	Het
Ptprs	T	A	17: 56,761,220 (GRCm39)	I116F	possibly damaging	Het
Rab1a	T	G	11: 20,173,169 (GRCm39)	V90G	probably damaging	Het
Rcor1	T	C	12: 111,068,102 (GRCm39)	V267A	probably benign	Het
Reep4	A	G	14: 70,784,678 (GRCm39)		probably null	Het
Rere	T	A	4: 150,699,779 (GRCm39)		probably benign	Het
Rin3	T	A	12: 102,353,823 (GRCm39)	Y743*	probably null	Het
Rprm	A	G	2: 53,975,067 (GRCm39)	S84P	probably damaging	Het
Sdhaf2	C	T	19: 10,494,383 (GRCm39)	E109K	probably damaging	Het
Sec31b	G	T	19: 44,523,225 (GRCm39)	Q24K	probably damaging	Het
Sema5a	T	C	15: 32,574,949 (GRCm39)		probably benign	Het
Sh3pxd2a	T	C	19: 47,256,186 (GRCm39)	Y844C	probably damaging	Het
Shmt2	A	C	10: 127,355,941 (GRCm39)	N134K	probably damaging	Het
Slc9a8	C	T	2: 167,266,125 (GRCm39)	A34V	probably benign	Het
Spidr	A	C	16: 15,957,936 (GRCm39)	S64A	possibly damaging	Het
Stk10	A	G	11: 32,567,882 (GRCm39)	T895A	probably benign	Het
Syne2	A	T	12: 76,080,365 (GRCm39)		probably benign	Het
Szt2	T	C	4: 118,230,149 (GRCm39)		probably null	Het
Tecpr1	A	T	5: 144,150,899 (GRCm39)	V303D	probably damaging	Het
Tet3	A	G	6: 83,350,776 (GRCm39)	Y1048H	probably damaging	Het
Tfb2m	A	G	1: 179,373,396 (GRCm39)	C101R	probably damaging	Het
Tg	T	C	15: 66,554,253 (GRCm39)	V556A	probably damaging	Het
Thbs4	A	C	13: 92,903,692 (GRCm39)	I441M	probably benign	Het
Thsd7a	A	T	6: 12,379,593 (GRCm39)	Y944N	probably damaging	Het
Tm9sf3	C	A	19: 41,236,331 (GRCm39)		probably benign	Het
Tmem145	T	C	7: 25,010,787 (GRCm39)	F359S	probably damaging	Het
Ttc21b	C	T	2: 66,053,142 (GRCm39)		probably benign	Het
Ttn	A	G	2: 76,579,880 (GRCm39)	V23671A	probably damaging	Het
Txnl4b	T	C	8: 110,298,103 (GRCm39)	I78T	probably benign	Het
Ubr4	C	A	4: 139,133,889 (GRCm39)	L762I	probably damaging	Het
Ubr4	T	C	4: 139,208,149 (GRCm39)		probably null	Het
Ugt1a8	C	T	1: 88,016,079 (GRCm39)	P164L	probably damaging	Het
Unc13b	T	C	4: 43,263,559 (GRCm39)	S1594P	probably damaging	Het
Utrn	A	T	10: 12,278,639 (GRCm39)	F912I	probably benign	Het
Vat1l	T	C	8: 114,963,319 (GRCm39)		probably benign	Het
Vmn1r50	T	A	6: 90,084,863 (GRCm39)	S203T	probably damaging	Het
Vmn2r4	A	T	3: 64,296,784 (GRCm39)	L667Q	probably damaging	Het
Vmn2r66	T	G	7: 84,656,023 (GRCm39)	Q331P	probably damaging	Het
Wdsub1	A	T	2: 59,708,669 (GRCm39)	V68D	possibly damaging	Het
Wnk2	C	G	13: 49,238,870 (GRCm39)	A564P	possibly damaging	Het
Wnk2	T	A	13: 49,238,872 (GRCm39)	K563M	probably damaging	Het
Zan	T	A	5: 137,468,580 (GRCm39)	H297L	probably damaging	Het
Zfp426	A	T	9: 20,381,327 (GRCm39)	H539Q	probably damaging	Het
Zfp488	T	A	14: 33,692,497 (GRCm39)	N222I	probably damaging	Het
Zfp536	T	A	7: 37,268,243 (GRCm39)	H391L	probably damaging	Het
Zp1	T	A	19: 10,893,571 (GRCm39)	N31I	probably damaging	Het

Other mutations in Piezo2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Piezo2	APN	18	63,250,770 (GRCm39)	missense	probably damaging	1.00
IGL01370:Piezo2	APN	18	63,155,531 (GRCm39)	missense	probably damaging	1.00
IGL01543:Piezo2	APN	18	63,203,101 (GRCm39)	missense	probably damaging	1.00
IGL01561:Piezo2	APN	18	63,257,685 (GRCm39)	missense	probably benign	0.03
IGL01568:Piezo2	APN	18	63,163,463 (GRCm39)	missense	probably benign	0.28
IGL01653:Piezo2	APN	18	63,315,904 (GRCm39)	splice site	probably benign
IGL01674:Piezo2	APN	18	63,160,630 (GRCm39)	missense	probably damaging	1.00
IGL01684:Piezo2	APN	18	63,216,241 (GRCm39)	missense	probably damaging	1.00
IGL01744:Piezo2	APN	18	63,175,859 (GRCm39)	missense	probably damaging	1.00
IGL01859:Piezo2	APN	18	63,225,915 (GRCm39)	missense	probably benign	0.10
IGL02183:Piezo2	APN	18	63,153,705 (GRCm39)	missense	probably benign	0.00
IGL02407:Piezo2	APN	18	63,279,915 (GRCm39)	missense	probably damaging	1.00
IGL02441:Piezo2	APN	18	63,205,933 (GRCm39)	missense	probably damaging	1.00
IGL02542:Piezo2	APN	18	63,165,995 (GRCm39)	missense	probably damaging	0.96
IGL02652:Piezo2	APN	18	63,157,546 (GRCm39)	missense	probably damaging	1.00
IGL02710:Piezo2	APN	18	63,207,730 (GRCm39)	missense	probably damaging	1.00
IGL02850:Piezo2	APN	18	63,153,704 (GRCm39)	missense	probably benign	0.18
IGL02851:Piezo2	APN	18	63,153,704 (GRCm39)	missense	probably benign	0.18
IGL02972:Piezo2	APN	18	63,197,856 (GRCm39)	splice site	probably benign
IGL03011:Piezo2	APN	18	63,257,731 (GRCm39)	missense	probably benign	0.03
IGL03078:Piezo2	APN	18	63,203,146 (GRCm39)	missense	probably damaging	1.00
IGL03114:Piezo2	APN	18	63,163,343 (GRCm39)	splice site	probably null
IGL03129:Piezo2	APN	18	63,248,043 (GRCm39)	missense	probably benign
IGL03143:Piezo2	APN	18	63,241,147 (GRCm39)	missense	probably damaging	0.99
IGL03202:Piezo2	APN	18	63,144,669 (GRCm39)	missense	probably damaging	1.00
IGL03227:Piezo2	APN	18	63,257,677 (GRCm39)	missense	probably damaging	1.00
IGL03228:Piezo2	APN	18	63,186,133 (GRCm39)	missense	probably damaging	1.00
IGL03230:Piezo2	APN	18	63,174,791 (GRCm39)	missense	probably damaging	1.00
IGL03242:Piezo2	APN	18	63,144,609 (GRCm39)	utr 3 prime	probably benign
IGL03291:Piezo2	APN	18	63,154,379 (GRCm39)	missense	probably damaging	1.00
IGL03301:Piezo2	APN	18	63,160,775 (GRCm39)	missense	probably damaging	1.00
Piccolo	UTSW	18	63,144,767 (GRCm39)	missense	probably damaging	1.00
sopranino	UTSW	18	63,157,537 (GRCm39)	missense	probably damaging	1.00
woodwind	UTSW	18	63,257,713 (GRCm39)	missense	possibly damaging	0.50
P0023:Piezo2	UTSW	18	63,519,271 (GRCm39)	splice site	probably benign
PIT4802001:Piezo2	UTSW	18	63,157,540 (GRCm39)	missense	probably damaging	1.00
R0070:Piezo2	UTSW	18	63,235,155 (GRCm39)	missense	probably damaging	1.00
R0416:Piezo2	UTSW	18	63,157,562 (GRCm39)	missense	probably damaging	1.00
R0486:Piezo2	UTSW	18	63,162,132 (GRCm39)	missense	probably damaging	1.00
R0498:Piezo2	UTSW	18	63,235,245 (GRCm39)	missense	possibly damaging	0.87
R0506:Piezo2	UTSW	18	63,160,615 (GRCm39)	missense	probably damaging	1.00
R0523:Piezo2	UTSW	18	63,155,552 (GRCm39)	missense	probably damaging	1.00
R0587:Piezo2	UTSW	18	63,155,497 (GRCm39)	missense	possibly damaging	0.82
R0626:Piezo2	UTSW	18	63,152,329 (GRCm39)	missense	probably damaging	0.97
R0734:Piezo2	UTSW	18	63,174,794 (GRCm39)	missense	probably damaging	1.00
R0784:Piezo2	UTSW	18	63,216,306 (GRCm39)	missense	probably damaging	1.00
R0973:Piezo2	UTSW	18	63,148,873 (GRCm39)	missense	probably damaging	1.00
R1183:Piezo2	UTSW	18	63,219,824 (GRCm39)	missense	probably damaging	1.00
R1344:Piezo2	UTSW	18	63,154,325 (GRCm39)	missense	probably damaging	1.00
R1474:Piezo2	UTSW	18	63,216,202 (GRCm39)	missense	probably damaging	1.00
R1571:Piezo2	UTSW	18	63,277,990 (GRCm39)	missense	possibly damaging	0.67
R1643:Piezo2	UTSW	18	63,215,986 (GRCm39)	missense	probably benign	0.03
R1649:Piezo2	UTSW	18	63,250,743 (GRCm39)	missense	probably benign	0.34
R1741:Piezo2	UTSW	18	63,154,244 (GRCm39)	missense	probably damaging	1.00
R1764:Piezo2	UTSW	18	63,257,713 (GRCm39)	missense	possibly damaging	0.50
R1793:Piezo2	UTSW	18	63,239,355 (GRCm39)	missense	possibly damaging	0.78
R1799:Piezo2	UTSW	18	63,241,158 (GRCm39)	missense	probably damaging	1.00
R1799:Piezo2	UTSW	18	63,165,911 (GRCm39)	critical splice donor site	probably null
R1868:Piezo2	UTSW	18	63,152,415 (GRCm39)	missense	probably damaging	1.00
R1879:Piezo2	UTSW	18	63,247,031 (GRCm39)	missense	probably damaging	1.00
R1962:Piezo2	UTSW	18	63,211,911 (GRCm39)	missense	probably damaging	0.98
R1990:Piezo2	UTSW	18	63,207,733 (GRCm39)	missense	probably null	1.00
R1991:Piezo2	UTSW	18	63,207,733 (GRCm39)	missense	probably null	1.00
R1992:Piezo2	UTSW	18	63,207,733 (GRCm39)	missense	probably null	1.00
R1995:Piezo2	UTSW	18	63,211,852 (GRCm39)	missense	probably damaging	1.00
R2004:Piezo2	UTSW	18	63,277,997 (GRCm39)	missense	probably damaging	1.00
R2011:Piezo2	UTSW	18	63,192,815 (GRCm39)	missense	probably damaging	1.00
R2029:Piezo2	UTSW	18	63,252,006 (GRCm39)	missense	possibly damaging	0.62
R2075:Piezo2	UTSW	18	63,214,805 (GRCm39)	missense	probably damaging	1.00
R2078:Piezo2	UTSW	18	63,250,791 (GRCm39)	missense	probably damaging	0.99
R2152:Piezo2	UTSW	18	63,247,112 (GRCm39)	missense	probably damaging	1.00
R2162:Piezo2	UTSW	18	63,214,733 (GRCm39)	critical splice donor site	probably null
R2183:Piezo2	UTSW	18	63,239,345 (GRCm39)	missense	probably damaging	1.00
R2230:Piezo2	UTSW	18	63,278,143 (GRCm39)	missense	probably damaging	1.00
R2231:Piezo2	UTSW	18	63,278,143 (GRCm39)	missense	probably damaging	1.00
R2406:Piezo2	UTSW	18	63,155,596 (GRCm39)	missense	probably damaging	1.00
R2431:Piezo2	UTSW	18	63,378,695 (GRCm39)	missense	possibly damaging	0.95
R2876:Piezo2	UTSW	18	63,186,106 (GRCm39)	missense	probably damaging	1.00
R2935:Piezo2	UTSW	18	63,279,914 (GRCm39)	missense	probably damaging	1.00
R3004:Piezo2	UTSW	18	63,157,506 (GRCm39)	nonsense	probably null
R3016:Piezo2	UTSW	18	63,175,903 (GRCm39)	missense	probably damaging	1.00
R3794:Piezo2	UTSW	18	63,214,864 (GRCm39)	missense	probably damaging	0.99
R3832:Piezo2	UTSW	18	63,214,733 (GRCm39)	critical splice donor site	probably null
R3833:Piezo2	UTSW	18	63,214,733 (GRCm39)	critical splice donor site	probably null
R3968:Piezo2	UTSW	18	63,144,767 (GRCm39)	missense	probably damaging	1.00
R3969:Piezo2	UTSW	18	63,144,767 (GRCm39)	missense	probably damaging	1.00
R3970:Piezo2	UTSW	18	63,144,767 (GRCm39)	missense	probably damaging	1.00
R4169:Piezo2	UTSW	18	63,183,675 (GRCm39)	missense	probably benign
R4181:Piezo2	UTSW	18	63,257,801 (GRCm39)	critical splice acceptor site	probably null
R4301:Piezo2	UTSW	18	63,217,911 (GRCm39)	missense	probably damaging	1.00
R4302:Piezo2	UTSW	18	63,257,801 (GRCm39)	critical splice acceptor site	probably null
R4475:Piezo2	UTSW	18	63,235,170 (GRCm39)	missense	probably damaging	1.00
R4493:Piezo2	UTSW	18	63,247,134 (GRCm39)	missense	probably damaging	0.98
R4519:Piezo2	UTSW	18	63,205,951 (GRCm39)	missense	probably damaging	1.00
R4539:Piezo2	UTSW	18	63,219,699 (GRCm39)	missense	probably damaging	1.00
R4687:Piezo2	UTSW	18	63,203,034 (GRCm39)	missense	probably damaging	1.00
R4732:Piezo2	UTSW	18	63,163,472 (GRCm39)	missense	probably damaging	1.00
R4733:Piezo2	UTSW	18	63,163,472 (GRCm39)	missense	probably damaging	1.00
R4825:Piezo2	UTSW	18	63,278,025 (GRCm39)	missense	probably damaging	0.98
R4899:Piezo2	UTSW	18	63,211,862 (GRCm39)	missense	possibly damaging	0.84
R4946:Piezo2	UTSW	18	63,290,333 (GRCm39)	missense	probably benign
R4961:Piezo2	UTSW	18	63,186,032 (GRCm39)	splice site	probably null
R4968:Piezo2	UTSW	18	63,278,042 (GRCm39)	nonsense	probably null
R4973:Piezo2	UTSW	18	63,207,751 (GRCm39)	missense	probably damaging	1.00
R4997:Piezo2	UTSW	18	63,216,184 (GRCm39)	missense	probably damaging	1.00
R5078:Piezo2	UTSW	18	63,157,607 (GRCm39)	missense	probably damaging	1.00
R5134:Piezo2	UTSW	18	63,207,691 (GRCm39)	missense	probably damaging	1.00
R5151:Piezo2	UTSW	18	63,163,480 (GRCm39)	missense	possibly damaging	0.72
R5209:Piezo2	UTSW	18	63,166,000 (GRCm39)	missense	probably damaging	1.00
R5367:Piezo2	UTSW	18	63,197,802 (GRCm39)	missense	probably damaging	1.00
R5401:Piezo2	UTSW	18	63,217,811 (GRCm39)	missense	possibly damaging	0.81
R5464:Piezo2	UTSW	18	63,278,176 (GRCm39)	missense	probably damaging	1.00
R5469:Piezo2	UTSW	18	63,160,935 (GRCm39)	missense	probably damaging	1.00
R5650:Piezo2	UTSW	18	63,144,792 (GRCm39)	missense	probably damaging	1.00
R5654:Piezo2	UTSW	18	63,278,162 (GRCm39)	missense	possibly damaging	0.94
R5677:Piezo2	UTSW	18	63,250,768 (GRCm39)	missense	probably benign	0.25
R5677:Piezo2	UTSW	18	63,250,767 (GRCm39)	missense	possibly damaging	0.94
R5792:Piezo2	UTSW	18	63,279,927 (GRCm39)	missense	probably damaging	1.00
R5874:Piezo2	UTSW	18	63,160,972 (GRCm39)	missense	probably damaging	1.00
R5877:Piezo2	UTSW	18	63,247,005 (GRCm39)	missense	probably benign	0.22
R6036:Piezo2	UTSW	18	63,248,019 (GRCm39)	nonsense	probably null
R6036:Piezo2	UTSW	18	63,248,019 (GRCm39)	nonsense	probably null
R6073:Piezo2	UTSW	18	63,145,716 (GRCm39)	missense	probably damaging	1.00
R6198:Piezo2	UTSW	18	63,290,281 (GRCm39)	nonsense	probably null
R6255:Piezo2	UTSW	18	63,254,341 (GRCm39)	missense	possibly damaging	0.75
R6259:Piezo2	UTSW	18	63,250,749 (GRCm39)	missense	possibly damaging	0.69
R6391:Piezo2	UTSW	18	63,239,364 (GRCm39)	missense	possibly damaging	0.79
R6446:Piezo2	UTSW	18	63,219,678 (GRCm39)	missense	probably damaging	1.00
R6465:Piezo2	UTSW	18	63,174,734 (GRCm39)	missense	possibly damaging	0.82
R6518:Piezo2	UTSW	18	63,239,342 (GRCm39)	missense	probably damaging	0.99
R6521:Piezo2	UTSW	18	63,154,399 (GRCm39)	missense	probably damaging	1.00
R6625:Piezo2	UTSW	18	63,154,333 (GRCm39)	missense	probably damaging	1.00
R6744:Piezo2	UTSW	18	63,165,960 (GRCm39)	nonsense	probably null
R6855:Piezo2	UTSW	18	63,223,950 (GRCm39)	critical splice donor site	probably null
R6927:Piezo2	UTSW	18	63,166,057 (GRCm39)	missense	probably damaging	1.00
R6980:Piezo2	UTSW	18	63,216,032 (GRCm39)	critical splice acceptor site	probably null
R7141:Piezo2	UTSW	18	63,278,181 (GRCm39)	nonsense	probably null
R7162:Piezo2	UTSW	18	63,257,780 (GRCm39)	missense	possibly damaging	0.50
R7331:Piezo2	UTSW	18	63,241,101 (GRCm39)	missense	probably damaging	0.99
R7382:Piezo2	UTSW	18	63,150,590 (GRCm39)	splice site	probably null
R7395:Piezo2	UTSW	18	63,160,634 (GRCm39)	missense	probably damaging	1.00
R7448:Piezo2	UTSW	18	63,157,543 (GRCm39)	missense	probably damaging	1.00
R7465:Piezo2	UTSW	18	63,145,794 (GRCm39)	missense	probably benign
R7517:Piezo2	UTSW	18	63,215,996 (GRCm39)	missense	possibly damaging	0.52
R7577:Piezo2	UTSW	18	63,186,081 (GRCm39)	missense	probably benign	0.01
R7612:Piezo2	UTSW	18	63,175,610 (GRCm39)	missense	probably benign	0.12
R7829:Piezo2	UTSW	18	63,246,947 (GRCm39)	critical splice donor site	probably null
R7835:Piezo2	UTSW	18	63,216,016 (GRCm39)	missense	probably benign	0.12
R8014:Piezo2	UTSW	18	63,216,271 (GRCm39)	missense	probably benign	0.02
R8055:Piezo2	UTSW	18	63,175,882 (GRCm39)	missense	probably damaging	0.99
R8062:Piezo2	UTSW	18	63,163,537 (GRCm39)	missense	possibly damaging	0.87
R8306:Piezo2	UTSW	18	63,208,801 (GRCm39)	missense	probably damaging	1.00
R8332:Piezo2	UTSW	18	63,145,857 (GRCm39)	missense	possibly damaging	0.67
R8355:Piezo2	UTSW	18	63,224,069 (GRCm39)	missense	probably damaging	1.00
R8383:Piezo2	UTSW	18	63,217,759 (GRCm39)	missense	probably damaging	0.97
R8455:Piezo2	UTSW	18	63,224,069 (GRCm39)	missense	probably damaging	1.00
R8501:Piezo2	UTSW	18	63,178,611 (GRCm39)	missense	probably damaging	0.99
R8523:Piezo2	UTSW	18	63,279,873 (GRCm39)	missense	probably damaging	0.99
R8692:Piezo2	UTSW	18	63,225,971 (GRCm39)	nonsense	probably null
R8708:Piezo2	UTSW	18	63,226,086 (GRCm39)	missense	probably damaging	1.00
R8726:Piezo2	UTSW	18	63,242,956 (GRCm39)	missense	probably benign
R8727:Piezo2	UTSW	18	63,242,956 (GRCm39)	missense	probably benign
R8810:Piezo2	UTSW	18	63,248,034 (GRCm39)	missense	probably benign	0.41
R8900:Piezo2	UTSW	18	63,248,096 (GRCm39)	missense	probably benign	0.04
R9037:Piezo2	UTSW	18	63,225,902 (GRCm39)	missense	probably benign	0.31
R9079:Piezo2	UTSW	18	63,157,537 (GRCm39)	missense	probably damaging	1.00
R9090:Piezo2	UTSW	18	63,208,790 (GRCm39)	missense	probably damaging	0.99
R9090:Piezo2	UTSW	18	63,163,450 (GRCm39)	missense	probably damaging	0.99
R9123:Piezo2	UTSW	18	63,178,589 (GRCm39)	missense	probably benign	0.00
R9125:Piezo2	UTSW	18	63,178,589 (GRCm39)	missense	probably benign	0.00
R9171:Piezo2	UTSW	18	63,178,550 (GRCm39)	missense	probably benign	0.04
R9194:Piezo2	UTSW	18	63,250,815 (GRCm39)	missense	probably benign	0.03
R9203:Piezo2	UTSW	18	63,290,302 (GRCm39)	missense	probably benign	0.00
R9209:Piezo2	UTSW	18	63,154,372 (GRCm39)	missense	probably damaging	1.00
R9261:Piezo2	UTSW	18	63,208,868 (GRCm39)	missense	possibly damaging	0.84
R9271:Piezo2	UTSW	18	63,163,450 (GRCm39)	missense	probably damaging	0.99
R9271:Piezo2	UTSW	18	63,208,790 (GRCm39)	missense	probably damaging	0.99
R9283:Piezo2	UTSW	18	63,157,637 (GRCm39)	missense	probably damaging	1.00
R9377:Piezo2	UTSW	18	63,162,156 (GRCm39)	missense	possibly damaging	0.48
R9499:Piezo2	UTSW	18	63,166,033 (GRCm39)	missense	possibly damaging	0.67
R9531:Piezo2	UTSW	18	63,235,236 (GRCm39)	missense	possibly damaging	0.95
R9551:Piezo2	UTSW	18	63,166,033 (GRCm39)	missense	possibly damaging	0.67
R9607:Piezo2	UTSW	18	63,519,347 (GRCm39)	start gained	probably benign
R9608:Piezo2	UTSW	18	63,280,016 (GRCm39)	missense	probably benign	0.09
R9617:Piezo2	UTSW	18	63,248,108 (GRCm39)	missense	probably benign	0.43
R9624:Piezo2	UTSW	18	63,197,767 (GRCm39)	missense	possibly damaging	0.88
X0017:Piezo2	UTSW	18	63,160,657 (GRCm39)	missense	probably damaging	0.99
X0022:Piezo2	UTSW	18	63,183,681 (GRCm39)	missense	probably benign	0.43
X0060:Piezo2	UTSW	18	63,150,648 (GRCm39)	missense	probably benign	0.09
Z1088:Piezo2	UTSW	18	63,203,065 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TGCCAACATTGTAAGAGCTTCAGGG -3'
(R):5'- TCATGATGACCGAGGATGCTCCAG -3'

Sequencing Primer
(F):5'- GGTGCCTTACGCCCTTG -3'
(R):5'- GGGCTGTCTGCCTATCAAAT -3'

Posted On

2013-06-12