Mutagenetix > Incidental Mutation

Incidental Mutation 'R6019:Vmn2r7'

478768

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r7

Ensembl Gene

ENSMUSG00000116028

Gene Name

vomeronasal 2, receptor 7

Synonyms

4933425M15Rik

MMRRC Submission

044193-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.115) question?

Stock #

R6019 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64598081-64627023 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 64623643 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 317 (T317A)

Ref Sequence

ENSEMBL: ENSMUSP00000124192 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000161972] [ENSMUST00000168072]

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000050123

Predicted Effect

probably damaging
Transcript: ENSMUST00000161972
AA Change: T317A

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000124192
Gene: ENSMUSG00000062200
AA Change: T317A

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	90	507	3.8e-77	PFAM
Pfam:NCD3G	549	602	3.4e-17	PFAM
Pfam:7tm_3	635	869	1.1e-47	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000168072
AA Change: T226A

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000131220
Gene: ENSMUSG00000116028
AA Change: T226A

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	5.1e-72	PFAM
Pfam:Peripla_BP_6	63	245	6.1e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	4.3e-76	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000177146

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.2%
20x: 91.1%

Validation Efficiency

96% (81/84)

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf3	C	T	16: 20,371,201 (GRCm39)	H436Y	possibly damaging	Het
Acad10	A	T	5: 121,772,864 (GRCm39)	H472Q	possibly damaging	Het
Ano8	G	A	8: 71,935,024 (GRCm39)	R393C	probably damaging	Het
Arhgap18	T	A	10: 26,736,646 (GRCm39)	V163E	probably damaging	Het
AW209491	C	T	13: 14,812,365 (GRCm39)	A406V	probably benign	Het
Brix1	C	T	15: 10,476,675 (GRCm39)	R267H	probably benign	Het
Cacng5	T	C	11: 107,775,214 (GRCm39)	M52V	probably benign	Het
Casz1	T	C	4: 149,031,495 (GRCm39)	C1249R	probably damaging	Het
Cenpj	C	T	14: 56,772,272 (GRCm39)	S1086N	probably benign	Het
Chi3l1	T	C	1: 134,117,310 (GRCm39)	F367S	probably benign	Het
Copg2	A	T	6: 30,787,868 (GRCm39)	I610N	possibly damaging	Het
Cpa6	T	C	1: 10,665,868 (GRCm39)	K57E	possibly damaging	Het
D5Ertd579e	C	T	5: 36,787,036 (GRCm39)	A111T	possibly damaging	Het
Dgat2	A	G	7: 98,803,838 (GRCm39)	M361T	probably benign	Het
Dnm3	G	T	1: 161,962,070 (GRCm39)	F46L	probably damaging	Het
Dph7	T	A	2: 24,853,552 (GRCm39)	C122*	probably null	Het
Dspp	A	T	5: 104,325,905 (GRCm39)	D756V	unknown	Het
Efcab3	T	C	11: 104,933,728 (GRCm39)		probably null	Het
Ep300	T	A	15: 81,525,583 (GRCm39)	M1469K	unknown	Het
Fsip2	T	C	2: 82,818,283 (GRCm39)	I4672T	possibly damaging	Het
Gapdh	A	T	6: 125,139,996 (GRCm39)	L67*	probably null	Het
Gpr75	A	G	11: 30,841,640 (GRCm39)	R182G	probably benign	Het
Gsr	G	T	8: 34,183,835 (GRCm39)	A366S	probably damaging	Het
Gypc	A	G	18: 32,663,248 (GRCm39)	I33T	probably damaging	Het
Hapln1	A	G	13: 89,756,219 (GRCm39)	D341G	probably benign	Het
Hnrnpd	A	G	5: 100,115,095 (GRCm39)	S148P	probably benign	Het
Hydin	A	T	8: 111,293,252 (GRCm39)	T3450S	probably benign	Het
Kif20b	T	C	19: 34,927,864 (GRCm39)	V1002A	probably benign	Het
Kif5c	T	C	2: 49,625,521 (GRCm39)	V597A	probably benign	Het
Kntc1	A	G	5: 123,900,579 (GRCm39)	T226A	probably benign	Het
Krt75	C	A	15: 101,482,158 (GRCm39)	V37L	probably benign	Het
L3mbtl2	A	G	15: 81,571,143 (GRCm39)	I668V	probably benign	Het
Lrp1b	G	A	2: 41,192,982 (GRCm39)	A480V	probably damaging	Het
Lrp1b	T	A	2: 41,366,821 (GRCm39)	D485V	probably damaging	Het
Lrrc37a	T	C	11: 103,347,422 (GRCm39)	H3091R	unknown	Het
Msi1	A	G	5: 115,589,550 (GRCm39)	Y361C	probably damaging	Het
Mtus1	G	T	8: 41,536,077 (GRCm39)	N546K	probably benign	Het
Mup17	T	A	4: 61,511,936 (GRCm39)	T113S	probably benign	Het
Myh11	T	A	16: 14,023,938 (GRCm39)	D1479V	probably damaging	Het
Ncor1	C	T	11: 62,263,987 (GRCm39)	E198K	probably benign	Het
Nrde2	A	G	12: 100,098,501 (GRCm39)	V722A	probably benign	Het
Or13a28	T	C	7: 140,217,925 (GRCm39)	F104L	probably benign	Het
Or51f23	C	T	7: 102,453,491 (GRCm39)	R269*	probably null	Het
Or5ac24	C	T	16: 59,165,798 (GRCm39)	D89N	possibly damaging	Het
Otog	A	G	7: 45,938,374 (GRCm39)	M2028V	probably benign	Het
Paox	T	A	7: 139,711,655 (GRCm39)	V169E	probably damaging	Het
Pcsk9	T	G	4: 106,314,073 (GRCm39)	D174A	probably benign	Het
Pde4b	A	G	4: 102,427,966 (GRCm39)	E41G	possibly damaging	Het
Pip4k2c	A	G	10: 127,034,943 (GRCm39)	I419T	probably damaging	Het
Plekhg3	G	T	12: 76,624,715 (GRCm39)	E1186*	probably null	Het
Pole	C	A	5: 110,472,380 (GRCm39)	P1548T	probably benign	Het
Pole	C	T	5: 110,472,381 (GRCm39)	P1548L	probably benign	Het
Polq	A	G	16: 36,882,126 (GRCm39)	E1430G	probably damaging	Het
Potefam3c	A	T	8: 69,881,966 (GRCm39)	C337S	probably benign	Het
Pramel47	G	A	5: 95,488,072 (GRCm39)	S2N	probably damaging	Het
Ptgr2	T	C	12: 84,344,920 (GRCm39)	S98P	probably damaging	Het
Ralgapa1	A	G	12: 55,730,827 (GRCm39)	Y1903H	possibly damaging	Het
Rasgrp1	T	C	2: 117,122,376 (GRCm39)	D338G	probably damaging	Het
Rif1	C	G	2: 51,985,856 (GRCm39)	L614V	probably damaging	Het
Rnase13	C	T	14: 52,159,860 (GRCm39)	C93Y	probably damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
S100z	T	A	13: 95,613,934 (GRCm39)	M59L	probably benign	Het
Ska1	T	A	18: 74,332,992 (GRCm39)	D142V	probably benign	Het
Slc16a3	T	C	11: 120,847,931 (GRCm39)		probably null	Het
Snd1	T	C	6: 28,880,233 (GRCm39)	V669A	probably benign	Het
Snrpd3	A	T	10: 75,368,029 (GRCm39)	T49S	probably damaging	Het
Sort1	T	C	3: 108,264,549 (GRCm39)	L856P	possibly damaging	Het
Srek1ip1	T	A	13: 104,970,830 (GRCm39)		probably null	Het
Ssrp1	T	C	2: 84,875,796 (GRCm39)	S552P	probably damaging	Het
Stab2	A	G	10: 86,838,886 (GRCm39)	V60A	probably benign	Het
Stard9	GCCC	GCC	2: 120,524,196 (GRCm39)		probably null	Het
Tll1	A	C	8: 64,494,525 (GRCm39)	H743Q	possibly damaging	Het
Tpo	G	T	12: 30,144,980 (GRCm39)	R590S	possibly damaging	Het
Trbv12-1	A	T	6: 41,090,780 (GRCm39)	T51S	probably benign	Het
Trbv30	C	T	6: 41,258,708 (GRCm39)	A40V	probably benign	Het
Tulp4	T	A	17: 6,283,490 (GRCm39)	V1173E	possibly damaging	Het
Upk1a	A	T	7: 30,311,810 (GRCm39)		probably null	Het
Vinac1	T	C	2: 128,879,610 (GRCm39)	Q772R	probably benign	Het
Vmn1r199	A	G	13: 22,566,769 (GRCm39)	D21G	possibly damaging	Het
Wdfy3	A	G	5: 101,997,289 (GRCm39)	V3112A	probably damaging	Het
Zbtb8os	T	C	4: 129,234,542 (GRCm39)	V40A	possibly damaging	Het
Zfp1002	T	A	2: 150,097,132 (GRCm39)	H99L	probably damaging	Het
Zwint	A	G	10: 72,492,685 (GRCm39)	K108E	possibly damaging	Het

Other mutations in Vmn2r7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00961:Vmn2r7	APN	3	64,623,234 (GRCm39)	missense	possibly damaging	0.60
IGL01762:Vmn2r7	APN	3	64,598,856 (GRCm39)	missense	probably benign	0.39
IGL01903:Vmn2r7	APN	3	64,626,864 (GRCm39)	missense	probably benign	0.00
IGL02263:Vmn2r7	APN	3	64,598,868 (GRCm39)	missense	probably damaging	1.00
IGL02458:Vmn2r7	APN	3	64,600,446 (GRCm39)	missense	probably damaging	0.97
IGL02593:Vmn2r7	APN	3	64,600,443 (GRCm39)	missense	probably damaging	1.00
IGL02797:Vmn2r7	APN	3	64,598,666 (GRCm39)	missense	possibly damaging	0.80
IGL03047:Vmn2r7	UTSW	3	64,614,639 (GRCm39)	missense	possibly damaging	0.81
PIT4504001:Vmn2r7	UTSW	3	64,623,397 (GRCm39)	missense	probably benign	0.01
R0193:Vmn2r7	UTSW	3	64,598,460 (GRCm39)	missense	probably damaging	1.00
R0329:Vmn2r7	UTSW	3	64,598,439 (GRCm39)	missense	probably damaging	1.00
R0609:Vmn2r7	UTSW	3	64,623,900 (GRCm39)	missense	probably benign	0.44
R0735:Vmn2r7	UTSW	3	64,623,788 (GRCm39)	missense	probably benign	0.02
R0941:Vmn2r7	UTSW	3	64,624,000 (GRCm39)	missense	probably benign
R1065:Vmn2r7	UTSW	3	64,614,559 (GRCm39)	missense	possibly damaging	0.82
R1378:Vmn2r7	UTSW	3	64,599,025 (GRCm39)	missense	possibly damaging	0.59
R1445:Vmn2r7	UTSW	3	64,632,223 (GRCm39)	missense	probably benign	0.01
R1506:Vmn2r7	UTSW	3	64,614,500 (GRCm39)	missense	probably benign
R1509:Vmn2r7	UTSW	3	64,623,881 (GRCm39)	nonsense	probably null
R1519:Vmn2r7	UTSW	3	64,623,876 (GRCm39)	missense	possibly damaging	0.95
R1706:Vmn2r7	UTSW	3	64,598,880 (GRCm39)	missense	possibly damaging	0.79
R2113:Vmn2r7	UTSW	3	64,599,025 (GRCm39)	missense	possibly damaging	0.59
R3725:Vmn2r7	UTSW	3	64,632,412 (GRCm39)	missense	possibly damaging	0.65
R3874:Vmn2r7	UTSW	3	64,627,032 (GRCm39)	missense	possibly damaging	0.69
R3902:Vmn2r7	UTSW	3	64,626,937 (GRCm39)	missense	possibly damaging	0.46
R4084:Vmn2r7	UTSW	3	64,600,414 (GRCm39)	missense	probably benign	0.08
R4117:Vmn2r7	UTSW	3	64,623,138 (GRCm39)	intron	probably benign
R4333:Vmn2r7	UTSW	3	64,598,199 (GRCm39)	missense	probably damaging	1.00
R4551:Vmn2r7	UTSW	3	64,598,110 (GRCm39)	missense	possibly damaging	0.86
R4643:Vmn2r7	UTSW	3	64,623,825 (GRCm39)	missense	probably damaging	1.00
R4654:Vmn2r7	UTSW	3	64,626,864 (GRCm39)	missense	probably benign	0.00
R5091:Vmn2r7	UTSW	3	64,598,205 (GRCm39)	missense	possibly damaging	0.63
R5109:Vmn2r7	UTSW	3	64,598,088 (GRCm39)	missense	probably null	0.84
R5372:Vmn2r7	UTSW	3	64,623,745 (GRCm39)	missense	probably damaging	1.00
R5415:Vmn2r7	UTSW	3	64,623,658 (GRCm39)	missense	probably benign	0.07
R5740:Vmn2r7	UTSW	3	64,614,654 (GRCm39)	missense	probably benign
R5977:Vmn2r7	UTSW	3	64,623,464 (GRCm39)	nonsense	probably null
R6058:Vmn2r7	UTSW	3	64,632,436 (GRCm39)	missense	probably benign	0.00
R6139:Vmn2r7	UTSW	3	64,623,339 (GRCm39)	missense	probably damaging	1.00
R6696:Vmn2r7	UTSW	3	64,614,495 (GRCm39)	missense	probably benign	0.01
R6887:Vmn2r7	UTSW	3	64,598,248 (GRCm39)	missense	probably damaging	1.00
R6918:Vmn2r7	UTSW	3	64,598,760 (GRCm39)	missense	probably benign	0.03
R6949:Vmn2r7	UTSW	3	64,598,542 (GRCm39)	missense	probably damaging	0.99
R6980:Vmn2r7	UTSW	3	64,623,987 (GRCm39)	missense	possibly damaging	0.67
R7196:Vmn2r7	UTSW	3	64,623,198 (GRCm39)	missense	probably benign	0.00
R7286:Vmn2r7	UTSW	3	64,598,301 (GRCm39)	missense	probably benign	0.00
R7455:Vmn2r7	UTSW	3	64,624,014 (GRCm39)	missense	probably benign
R7557:Vmn2r7	UTSW	3	64,632,394 (GRCm39)	missense	probably benign
R7864:Vmn2r7	UTSW	3	64,598,947 (GRCm39)	missense	probably benign	0.10
R8046:Vmn2r7	UTSW	3	64,614,479 (GRCm39)	missense	probably damaging	1.00
R8068:Vmn2r7	UTSW	3	64,623,507 (GRCm39)	missense	probably benign	0.01
R8252:Vmn2r7	UTSW	3	64,600,527 (GRCm39)	missense	probably benign	0.00
R8814:Vmn2r7	UTSW	3	64,623,984 (GRCm39)	missense	probably benign	0.14
R9497:Vmn2r7	UTSW	3	64,614,474 (GRCm39)	missense	probably benign	0.04
R9722:Vmn2r7	UTSW	3	64,598,407 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAAGGCACAGTGCTGTTGGG -3'
(R):5'- GTGCCAACCTTTGTGTTGC -3'

Sequencing Primer
(F):5'- GCCAGGTACAGTTAAAAGCAGTTTG -3'
(R):5'- GCTTTCTCTGAAACCATTCCAAAAG -3'

Posted On

2017-06-26