Incidental Mutation 'R5983:Rbm5'
ID |
481527 |
Institutional Source |
Beutler Lab
|
Gene Symbol |
Rbm5
|
Ensembl Gene |
ENSMUSG00000032580 |
Gene Name |
RNA binding motif protein 5 |
Synonyms |
D030069N10Rik |
MMRRC Submission |
044164-MU
|
Accession Numbers |
|
Essential gene? |
Probably essential
(E-score: 0.928)
|
Stock # |
R5983 (G1)
|
Quality Score |
225.009 |
Status
|
Validated
|
Chromosome |
9 |
Chromosomal Location |
107617694-107648228 bp(-) (GRCm39) |
Type of Mutation |
missense |
DNA Base Change (assembly) |
G to A
at 107622141 bp (GRCm39)
|
Zygosity |
Heterozygous |
Amino Acid Change |
Proline to Leucine
at position 611
(P611L)
|
Ref Sequence |
ENSEMBL: ENSMUSP00000138379
(fasta)
|
Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000035199]
[ENSMUST00000182371]
[ENSMUST00000182421]
[ENSMUST00000182659]
|
AlphaFold |
Q91YE7 |
Predicted Effect |
probably damaging
Transcript: ENSMUST00000035199
AA Change: P610L
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
SMART Domains |
Protein: ENSMUSP00000035199 Gene: ENSMUSG00000032580 AA Change: P610L
Domain | Start | End | E-Value | Type |
low complexity region
|
21 |
74 |
N/A |
INTRINSIC |
RRM
|
99 |
174 |
5.98e-13 |
SMART |
ZnF_RBZ
|
183 |
207 |
1.53e-6 |
SMART |
RRM
|
232 |
311 |
9.5e-7 |
SMART |
low complexity region
|
329 |
355 |
N/A |
INTRINSIC |
low complexity region
|
362 |
385 |
N/A |
INTRINSIC |
low complexity region
|
393 |
410 |
N/A |
INTRINSIC |
low complexity region
|
425 |
446 |
N/A |
INTRINSIC |
Blast:RRM_2
|
483 |
506 |
1e-5 |
BLAST |
low complexity region
|
520 |
536 |
N/A |
INTRINSIC |
low complexity region
|
569 |
581 |
N/A |
INTRINSIC |
low complexity region
|
627 |
641 |
N/A |
INTRINSIC |
ZnF_C2H2
|
647 |
672 |
5.2e0 |
SMART |
coiled coil region
|
673 |
707 |
N/A |
INTRINSIC |
G_patch
|
741 |
787 |
1.85e-21 |
SMART |
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000182026
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000182215
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000182332
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000182371
|
SMART Domains |
Protein: ENSMUSP00000138119 Gene: ENSMUSG00000032580
Domain | Start | End | E-Value | Type |
low complexity region
|
21 |
74 |
N/A |
INTRINSIC |
SCOP:d1h6kx_
|
96 |
138 |
6e-3 |
SMART |
Blast:RRM
|
99 |
136 |
3e-19 |
BLAST |
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000182421
|
SMART Domains |
Protein: ENSMUSP00000138778 Gene: ENSMUSG00000032580
Domain | Start | End | E-Value | Type |
low complexity region
|
21 |
59 |
N/A |
INTRINSIC |
|
Predicted Effect |
probably damaging
Transcript: ENSMUST00000182659
AA Change: P611L
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
SMART Domains |
Protein: ENSMUSP00000138379 Gene: ENSMUSG00000032580 AA Change: P611L
Domain | Start | End | E-Value | Type |
low complexity region
|
21 |
74 |
N/A |
INTRINSIC |
RRM
|
99 |
174 |
5.98e-13 |
SMART |
ZnF_RBZ
|
183 |
207 |
1.53e-6 |
SMART |
RRM
|
232 |
311 |
9.5e-7 |
SMART |
low complexity region
|
329 |
355 |
N/A |
INTRINSIC |
low complexity region
|
362 |
385 |
N/A |
INTRINSIC |
low complexity region
|
393 |
410 |
N/A |
INTRINSIC |
low complexity region
|
425 |
446 |
N/A |
INTRINSIC |
Blast:RRM_2
|
483 |
506 |
1e-5 |
BLAST |
low complexity region
|
520 |
536 |
N/A |
INTRINSIC |
low complexity region
|
569 |
581 |
N/A |
INTRINSIC |
low complexity region
|
627 |
641 |
N/A |
INTRINSIC |
ZnF_C2H2
|
647 |
672 |
5.2e0 |
SMART |
coiled coil region
|
673 |
707 |
N/A |
INTRINSIC |
G_patch
|
741 |
787 |
1.85e-21 |
SMART |
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000183307
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000182792
|
Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000194801
|
Predicted Effect |
probably benign
Transcript: ENSMUST00000194400
|
Meta Mutation Damage Score |
0.1907 |
Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.5%
- 10x: 97.6%
- 20x: 92.6%
|
Validation Efficiency |
100% (29/29) |
MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011] PHENOTYPE: Mice homozygous for an ENU-induced allele exhibit male infertility with azoospermia, male germ cell apoptosis, round spermatid arrest and spermatid differentiation arrest. [provided by MGI curators]
|
Allele List at MGI |
|
Other mutations in this stock |
Total: 28 list
Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
Abcc2 |
T |
A |
19: 43,807,942 (GRCm39) |
M861K |
probably benign |
Het |
Abhd18 |
T |
A |
3: 40,864,979 (GRCm39) |
F73L |
probably damaging |
Het |
Abraxas1 |
A |
G |
5: 100,955,777 (GRCm39) |
V237A |
probably benign |
Het |
Adam26b |
T |
C |
8: 43,974,378 (GRCm39) |
Y208C |
probably damaging |
Het |
Aire |
A |
G |
10: 77,878,903 (GRCm39) |
L48P |
probably damaging |
Het |
Bach2 |
A |
T |
4: 32,563,324 (GRCm39) |
E597V |
probably damaging |
Het |
Ccdc170 |
A |
T |
10: 4,470,851 (GRCm39) |
R232* |
probably null |
Het |
Cfap100 |
A |
C |
6: 90,396,373 (GRCm39) |
|
probably benign |
Het |
Dppa2 |
G |
A |
16: 48,136,204 (GRCm39) |
M185I |
probably benign |
Het |
Ecsit |
C |
T |
9: 21,989,443 (GRCm39) |
|
probably null |
Het |
Fbxw26 |
A |
G |
9: 109,547,033 (GRCm39) |
I464T |
possibly damaging |
Het |
Grm8 |
T |
C |
6: 27,760,220 (GRCm39) |
K370R |
probably benign |
Het |
Ide |
C |
T |
19: 37,249,549 (GRCm39) |
|
probably null |
Het |
Katnip |
T |
A |
7: 125,449,545 (GRCm39) |
W870R |
probably damaging |
Het |
Morn3 |
T |
C |
5: 123,175,851 (GRCm39) |
D179G |
possibly damaging |
Het |
Nkapd1 |
A |
G |
9: 50,519,142 (GRCm39) |
S157P |
probably damaging |
Het |
Nvl |
A |
T |
1: 180,964,471 (GRCm39) |
D102E |
probably benign |
Het |
Or5an10 |
T |
A |
19: 12,276,467 (GRCm39) |
I10F |
probably benign |
Het |
Rsph9 |
A |
G |
17: 46,440,406 (GRCm39) |
M230T |
probably benign |
Het |
Sacs |
G |
A |
14: 61,442,648 (GRCm39) |
V1565M |
probably damaging |
Het |
Serpina3n |
T |
C |
12: 104,375,288 (GRCm39) |
L120P |
probably damaging |
Het |
Spsb2 |
A |
C |
6: 124,786,711 (GRCm39) |
E148A |
probably benign |
Het |
Tmc2 |
T |
A |
2: 130,089,896 (GRCm39) |
M627K |
probably damaging |
Het |
Tmem147 |
T |
C |
7: 30,427,484 (GRCm39) |
D111G |
probably damaging |
Het |
Ttn |
A |
G |
2: 76,572,725 (GRCm39) |
V17729A |
possibly damaging |
Het |
Vmn2r104 |
G |
A |
17: 20,261,970 (GRCm39) |
P387S |
probably damaging |
Het |
Zfp940 |
C |
T |
7: 29,544,477 (GRCm39) |
V477M |
possibly damaging |
Het |
Zw10 |
A |
G |
9: 48,988,745 (GRCm39) |
|
probably null |
Het |
|
Other mutations in Rbm5 |
Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
IGL02041:Rbm5
|
APN |
9 |
107,633,045 (GRCm39) |
unclassified |
probably benign |
|
IGL02319:Rbm5
|
APN |
9 |
107,621,064 (GRCm39) |
nonsense |
probably null |
|
IGL02567:Rbm5
|
APN |
9 |
107,621,473 (GRCm39) |
missense |
probably damaging |
1.00 |
IGL02868:Rbm5
|
APN |
9 |
107,626,899 (GRCm39) |
splice site |
probably benign |
|
IGL02943:Rbm5
|
APN |
9 |
107,621,542 (GRCm39) |
missense |
probably damaging |
1.00 |
IGL02959:Rbm5
|
APN |
9 |
107,631,384 (GRCm39) |
unclassified |
probably benign |
|
IGL03029:Rbm5
|
APN |
9 |
107,631,652 (GRCm39) |
missense |
possibly damaging |
0.46 |
PIT4403001:Rbm5
|
UTSW |
9 |
107,637,535 (GRCm39) |
missense |
probably damaging |
1.00 |
R0001:Rbm5
|
UTSW |
9 |
107,619,623 (GRCm39) |
missense |
probably damaging |
1.00 |
R0242:Rbm5
|
UTSW |
9 |
107,628,907 (GRCm39) |
splice site |
probably benign |
|
R1170:Rbm5
|
UTSW |
9 |
107,619,696 (GRCm39) |
missense |
probably damaging |
0.99 |
R1239:Rbm5
|
UTSW |
9 |
107,630,165 (GRCm39) |
unclassified |
probably benign |
|
R1297:Rbm5
|
UTSW |
9 |
107,621,441 (GRCm39) |
missense |
probably damaging |
1.00 |
R1719:Rbm5
|
UTSW |
9 |
107,621,112 (GRCm39) |
critical splice acceptor site |
probably null |
|
R1764:Rbm5
|
UTSW |
9 |
107,644,763 (GRCm39) |
nonsense |
probably null |
|
R1826:Rbm5
|
UTSW |
9 |
107,619,613 (GRCm39) |
missense |
probably damaging |
0.98 |
R1863:Rbm5
|
UTSW |
9 |
107,627,718 (GRCm39) |
missense |
possibly damaging |
0.70 |
R4112:Rbm5
|
UTSW |
9 |
107,642,796 (GRCm39) |
missense |
probably damaging |
1.00 |
R4207:Rbm5
|
UTSW |
9 |
107,627,682 (GRCm39) |
missense |
probably benign |
|
R4343:Rbm5
|
UTSW |
9 |
107,629,395 (GRCm39) |
missense |
probably damaging |
1.00 |
R4441:Rbm5
|
UTSW |
9 |
107,626,887 (GRCm39) |
intron |
probably benign |
|
R4621:Rbm5
|
UTSW |
9 |
107,631,345 (GRCm39) |
missense |
probably damaging |
1.00 |
R4767:Rbm5
|
UTSW |
9 |
107,622,412 (GRCm39) |
missense |
probably damaging |
1.00 |
R5090:Rbm5
|
UTSW |
9 |
107,637,511 (GRCm39) |
splice site |
probably benign |
|
R5404:Rbm5
|
UTSW |
9 |
107,642,752 (GRCm39) |
missense |
probably damaging |
1.00 |
R5876:Rbm5
|
UTSW |
9 |
107,637,525 (GRCm39) |
missense |
probably damaging |
1.00 |
R5959:Rbm5
|
UTSW |
9 |
107,629,339 (GRCm39) |
missense |
probably benign |
0.02 |
R5984:Rbm5
|
UTSW |
9 |
107,622,141 (GRCm39) |
missense |
probably damaging |
1.00 |
R7436:Rbm5
|
UTSW |
9 |
107,627,593 (GRCm39) |
splice site |
probably null |
|
R7447:Rbm5
|
UTSW |
9 |
107,623,378 (GRCm39) |
missense |
probably damaging |
1.00 |
R7867:Rbm5
|
UTSW |
9 |
107,628,930 (GRCm39) |
missense |
probably benign |
0.02 |
R9377:Rbm5
|
UTSW |
9 |
107,627,252 (GRCm39) |
missense |
probably benign |
0.07 |
R9602:Rbm5
|
UTSW |
9 |
107,629,152 (GRCm39) |
missense |
probably benign |
|
R9694:Rbm5
|
UTSW |
9 |
107,622,152 (GRCm39) |
missense |
probably benign |
|
|
Predicted Primers |
PCR Primer
(F):5'- CAGGCTTCATTCATCGGTCC -3'
(R):5'- TTGCCAAAGACATGGAACGC -3'
Sequencing Primer
(F):5'- GGTCCATCTCACCTGATCTCAG -3'
(R):5'- GCTTTCAACCTGTCAATTCATTGAG -3'
|
Posted On |
2017-06-26 |