Mutagenetix > Incidental Mutation

Incidental Mutation 'R5985:Ankrd10'

481606

Institutional Source

Beutler Lab

Gene Symbol

Ankrd10

Ensembl Gene

ENSMUSG00000031508

Gene Name

ankyrin repeat domain 10

Synonyms

4833425P12Rik

MMRRC Submission

044165-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R5985 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

11661583-11685757 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 11669077 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 225 (K225*)

Ref Sequence

ENSEMBL: ENSMUSP00000131657 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033905] [ENSMUST00000169782] [ENSMUST00000209915] [ENSMUST00000210530] [ENSMUST00000211174]

AlphaFold

Q99LW0

Predicted Effect

probably null
Transcript: ENSMUST00000033905
AA Change: K225*

SMART Domains

Protein: ENSMUSP00000033905
Gene: ENSMUSG00000031508
AA Change: K225*

Domain	Start	End	E-Value	Type
Blast:ANK	20	49	2e-10	BLAST
ANK	56	85	2.66e-5	SMART
ANK	90	119	6.46e-4	SMART
ANK	123	152	1.03e-2	SMART
Blast:ANK	156	185	6e-6	BLAST

Predicted Effect

probably null
Transcript: ENSMUST00000169782
AA Change: K225*

SMART Domains

Protein: ENSMUSP00000131657
Gene: ENSMUSG00000031508
AA Change: K225*

Domain	Start	End	E-Value	Type
Blast:ANK	20	49	1e-10	BLAST
ANK	56	85	2.66e-5	SMART
ANK	90	119	6.46e-4	SMART
ANK	123	152	1.03e-2	SMART
Blast:ANK	156	185	4e-6	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000209915

Predicted Effect

probably benign
Transcript: ENSMUST00000210530

Predicted Effect

probably benign
Transcript: ENSMUST00000211174

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211445

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211690

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.2%

Validation Efficiency

96% (47/49)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	C	11: 9,241,628 (GRCm39)	I1164L	probably benign	Het
Actn1	C	A	12: 80,215,169 (GRCm39)	G864V	probably damaging	Het
Adgrf1	T	C	17: 43,604,146 (GRCm39)	I34T	probably benign	Het
Ankrd29	G	A	18: 12,412,832 (GRCm39)	A115V	probably damaging	Het
Armh3	A	T	19: 45,808,763 (GRCm39)	Y643N	probably damaging	Het
Atg2a	A	G	19: 6,304,667 (GRCm39)	T1175A	probably damaging	Het
Atp12a	A	G	14: 56,621,798 (GRCm39)	D781G	probably damaging	Het
B4galnt3	G	A	6: 120,187,119 (GRCm39)	P759S	probably damaging	Het
Card10	T	C	15: 78,675,411 (GRCm39)	I448V	probably benign	Het
Col15a1	T	C	4: 47,284,507 (GRCm39)	F821L	probably damaging	Het
Cpeb3	T	C	19: 37,064,952 (GRCm39)	Y498C	probably damaging	Het
Defb37	A	T	8: 19,036,347 (GRCm39)	C58S	probably damaging	Het
Faap20	T	C	4: 155,334,797 (GRCm39)		probably benign	Het
Frem1	C	T	4: 82,884,287 (GRCm39)	V1204I	probably benign	Het
Gal3st2	T	C	1: 93,801,335 (GRCm39)	V46A	possibly damaging	Het
Gan	C	T	8: 117,922,557 (GRCm39)	S430L	possibly damaging	Het
Gas2	T	A	7: 51,593,424 (GRCm39)	I168K	probably damaging	Het
Gli3	A	G	13: 15,898,140 (GRCm39)	D740G	probably damaging	Het
Gm3898	G	A	9: 43,741,329 (GRCm39)		noncoding transcript	Het
Gria4	T	C	9: 4,503,593 (GRCm39)	Q341R	probably damaging	Het
Herpud1	G	A	8: 95,117,422 (GRCm39)	R2Q	probably damaging	Het
Klf15	G	A	6: 90,443,703 (GRCm39)	G93R	possibly damaging	Het
Mtmr7	A	G	8: 41,004,873 (GRCm39)	F568L	probably benign	Het
Myod1	A	G	7: 46,027,222 (GRCm39)	Y229C	probably damaging	Het
Or12e14	A	G	2: 87,187,949 (GRCm39)	I54V	probably benign	Het
Or5l13	A	G	2: 87,779,665 (GRCm39)	M304T	probably benign	Het
Phf11b	A	G	14: 59,559,027 (GRCm39)	L235P	possibly damaging	Het
Pira1	C	A	7: 3,740,316 (GRCm39)	G302C	probably damaging	Het
Plekha6	G	C	1: 133,200,045 (GRCm39)	R208P	possibly damaging	Het
Prox1	A	T	1: 189,879,152 (GRCm39)	F675L	possibly damaging	Het
Rnf144a	C	T	12: 26,367,779 (GRCm39)	E176K	probably benign	Het
Safb2	T	C	17: 56,870,181 (GRCm39)	E965G	possibly damaging	Het
Sik3	A	G	9: 46,122,973 (GRCm39)	N874S	probably damaging	Het
Slitrk3	T	C	3: 72,958,233 (GRCm39)	I180V	probably damaging	Het
Speg	T	C	1: 75,383,328 (GRCm39)	V1141A	possibly damaging	Het
Spns1	G	A	7: 125,975,902 (GRCm39)	T84I	probably benign	Het
Syne2	C	G	12: 76,012,933 (GRCm39)	P2709R	probably damaging	Het
Terb1	A	T	8: 105,178,439 (GRCm39)	S662T	probably benign	Het
Terb1	A	G	8: 105,208,948 (GRCm39)	S377P	probably damaging	Het
Tph1	A	G	7: 46,303,205 (GRCm39)	Y258H	probably damaging	Het
Trip12	T	A	1: 84,703,492 (GRCm39)	E1881D	probably damaging	Het
Trpc7	A	G	13: 56,958,358 (GRCm39)	L412P	probably damaging	Het
Ttll13	C	T	7: 79,904,386 (GRCm39)	A337V	probably damaging	Het
Ugt2b1	T	C	5: 87,067,527 (GRCm39)	K344E	possibly damaging	Het
Vmn1r36	T	A	6: 66,693,855 (GRCm39)	I7F	probably benign	Het
Vmn2r14	A	T	5: 109,368,082 (GRCm39)	N303K	possibly damaging	Het
Vmn2r25	A	G	6: 123,800,587 (GRCm39)	V585A	probably benign	Het
Zan	A	C	5: 137,444,299 (GRCm39)		probably null	Het
Zfp423	A	T	8: 88,508,774 (GRCm39)	N502K	possibly damaging	Het
Zfp473	C	T	7: 44,382,752 (GRCm39)	R526Q	probably damaging	Het

Other mutations in Ankrd10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01480:Ankrd10	APN	8	11,685,592 (GRCm39)	missense	probably benign
IGL03029:Ankrd10	APN	8	11,669,304 (GRCm39)	splice site	probably null
R0098:Ankrd10	UTSW	8	11,662,560 (GRCm39)	missense	probably benign	0.01
R0393:Ankrd10	UTSW	8	11,685,482 (GRCm39)	missense	possibly damaging	0.58
R2870:Ankrd10	UTSW	8	11,665,682 (GRCm39)	missense	probably damaging	0.98
R2870:Ankrd10	UTSW	8	11,665,682 (GRCm39)	missense	probably damaging	0.98
R4176:Ankrd10	UTSW	8	11,662,644 (GRCm39)	missense	probably benign	0.00
R6999:Ankrd10	UTSW	8	11,669,106 (GRCm39)	missense	probably damaging	1.00
R7108:Ankrd10	UTSW	8	11,662,624 (GRCm39)	missense	probably damaging	1.00
R7322:Ankrd10	UTSW	8	11,665,841 (GRCm39)	missense	probably damaging	0.97
R7522:Ankrd10	UTSW	8	11,682,910 (GRCm39)	missense	probably damaging	1.00
R7559:Ankrd10	UTSW	8	11,662,548 (GRCm39)	missense	probably damaging	0.97
R7629:Ankrd10	UTSW	8	11,665,769 (GRCm39)	missense	probably benign	0.13
R8263:Ankrd10	UTSW	8	11,665,707 (GRCm39)	missense	probably benign	0.22
R8493:Ankrd10	UTSW	8	11,678,518 (GRCm39)	nonsense	probably null
R8713:Ankrd10	UTSW	8	11,678,491 (GRCm39)	missense	probably damaging	1.00
R8897:Ankrd10	UTSW	8	11,665,788 (GRCm39)	missense	possibly damaging	0.61

Predicted Primers

PCR Primer
(F):5'- TCTGACAAGCGGGACATATTACAG -3'
(R):5'- CAGCCTGAGAAATGCCAGTG -3'

Sequencing Primer
(F):5'- GTGTGACACAGGGCAGTACTTC -3'
(R):5'- GCCTGACAGCAGCAGACATTG -3'

Posted On

2017-06-26