Mutagenetix > Incidental Mutation

Incidental Mutation 'R5991:Gpr158'

481990

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

MMRRC Submission

044171-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5991 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21372378-21835355 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 21373319 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 85 (Y85H)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

probably damaging
Transcript: ENSMUST00000055946
AA Change: Y85H

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: Y85H

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133391

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155760

Meta Mutation Damage Score

0.3338

Coding Region Coverage

1x: 99.8%
3x: 99.4%
10x: 97.0%
20x: 90.5%

Validation Efficiency

96% (53/55)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars1	G	A	8: 111,777,032 (GRCm39)	R683Q	probably damaging	Het
Adam8	T	A	7: 139,570,200 (GRCm39)	H69L	probably damaging	Het
Bbs2	G	T	8: 94,824,914 (GRCm39)	R23S	probably benign	Het
Bod1l	A	T	5: 41,974,206 (GRCm39)	C2369*	probably null	Het
Capn11	G	T	17: 45,970,278 (GRCm39)		probably null	Het
Carmil2	A	G	8: 106,418,023 (GRCm39)	Q679R	probably null	Het
Cep120	T	C	18: 53,854,870 (GRCm39)	I422V	probably benign	Het
Cnot7	A	C	8: 40,948,696 (GRCm39)		probably null	Het
Col1a1	T	C	11: 94,828,745 (GRCm39)	V29A	unknown	Het
Dnttip1	A	T	2: 164,596,100 (GRCm39)	Q115L	possibly damaging	Het
Dyrk4	A	G	6: 126,857,188 (GRCm39)	V497A	probably benign	Het
Ep300	A	G	15: 81,532,667 (GRCm39)	D1635G	unknown	Het
Ercc5	T	A	1: 44,219,990 (GRCm39)	C1087*	probably null	Het
Fap	A	T	2: 62,348,865 (GRCm39)	M517K	probably damaging	Het
Focad	C	A	4: 88,319,256 (GRCm39)	D1574E	possibly damaging	Het
Fsip2	C	T	2: 82,820,812 (GRCm39)	P5515L	probably benign	Het
Hc	A	G	2: 34,896,117 (GRCm39)	V1222A	possibly damaging	Het
Hipk3	C	T	2: 104,268,328 (GRCm39)	M546I	probably damaging	Het
Jup	T	C	11: 100,270,395 (GRCm39)	N371D	possibly damaging	Het
Ltbp4	A	G	7: 27,008,741 (GRCm39)	Y1262H	probably damaging	Het
Lyar	A	G	5: 38,385,209 (GRCm39)	E83G	probably damaging	Het
Lyg2	C	T	1: 37,954,800 (GRCm39)		probably null	Het
Mtmr11	T	A	3: 96,075,905 (GRCm39)		probably null	Het
Myct1	A	T	10: 5,554,426 (GRCm39)	R98*	probably null	Het
Nol11	T	C	11: 107,061,971 (GRCm39)	T612A	probably benign	Het
Or2l5	T	C	16: 19,333,507 (GRCm39)	N293S	probably damaging	Het
Or4b1d	T	A	2: 89,968,578 (GRCm39)	K302*	probably null	Het
Or4c113	C	T	2: 88,885,126 (GRCm39)	V215I	probably benign	Het
Or4f61	C	T	2: 111,922,960 (GRCm39)	V29M	probably benign	Het
Or5m9	A	G	2: 85,877,254 (GRCm39)	I143V	probably benign	Het
Parp14	G	A	16: 35,661,827 (GRCm39)	P1403S	probably benign	Het
Pcdhb3	T	C	18: 37,434,561 (GRCm39)	S176P	probably benign	Het
Pdcd1lg2	C	T	19: 29,431,867 (GRCm39)	R212W	probably benign	Het
Plin3	T	C	17: 56,593,576 (GRCm39)	Y46C	probably damaging	Het
Rfx7	G	A	9: 72,526,820 (GRCm39)	E1337K	possibly damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Ryr1	G	T	7: 28,804,035 (GRCm39)	N584K	probably damaging	Het
Scmh1	T	C	4: 120,379,817 (GRCm39)	V544A	probably benign	Het
Sdk2	G	A	11: 113,834,080 (GRCm39)	T34M	probably damaging	Het
Serpinb9e	T	A	13: 33,443,790 (GRCm39)	L270H	probably damaging	Het
Shoc2	T	A	19: 53,991,480 (GRCm39)	N248K	probably damaging	Het
Sirpd	G	T	3: 15,385,818 (GRCm39)	A28E	probably benign	Het
Slc25a4	T	C	8: 46,662,373 (GRCm39)	Y95C	probably damaging	Het
Slc4a1ap	A	G	5: 31,691,413 (GRCm39)	N403S	possibly damaging	Het
Spink10	T	A	18: 62,790,956 (GRCm39)	W7R	probably null	Het
Ssrp1	A	G	2: 84,872,640 (GRCm39)	K426R	possibly damaging	Het
Tbc1d24	G	A	17: 24,428,043 (GRCm39)		probably benign	Het
Tead3	A	T	17: 28,553,352 (GRCm39)		probably null	Het
Timd4	A	G	11: 46,734,030 (GRCm39)	*344W	probably null	Het
Tmem181a	T	A	17: 6,339,312 (GRCm39)	W115R	probably damaging	Het
Tmem245	G	A	4: 56,916,733 (GRCm39)	T65I	probably damaging	Het
Vmn1r12	G	C	6: 57,136,640 (GRCm39)	V202L	probably damaging	Het
Vmn1r80	A	G	7: 11,927,423 (GRCm39)	T178A	probably benign	Het
Vps33b	T	A	7: 79,933,162 (GRCm39)		probably null	Het
Zmym6	T	A	4: 127,002,266 (GRCm39)		probably null	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21,373,494 (GRCm39)	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21,751,606 (GRCm39)	splice site	probably benign
IGL00706:Gpr158	APN	2	21,751,584 (GRCm39)	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21,831,629 (GRCm39)	nonsense	probably null
IGL00885:Gpr158	APN	2	21,653,832 (GRCm39)	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21,373,842 (GRCm39)	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21,831,909 (GRCm39)	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21,788,101 (GRCm39)	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21,373,511 (GRCm39)	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21,831,407 (GRCm39)	missense	probably benign
IGL02681:Gpr158	APN	2	21,820,441 (GRCm39)	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21,831,638 (GRCm39)	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21,831,890 (GRCm39)	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21,787,972 (GRCm39)	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21,831,057 (GRCm39)	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21,831,682 (GRCm39)	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21,815,479 (GRCm39)	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21,831,528 (GRCm39)	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21,830,019 (GRCm39)	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21,820,480 (GRCm39)	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21,831,125 (GRCm39)	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21,820,505 (GRCm39)	splice site	probably null
R1609:Gpr158	UTSW	2	21,788,104 (GRCm39)	missense	possibly damaging	0.61
R1741:Gpr158	UTSW	2	21,832,359 (GRCm39)	missense	probably benign	0.00
R1827:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R1854:Gpr158	UTSW	2	21,373,935 (GRCm39)	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21,820,426 (GRCm39)	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21,832,325 (GRCm39)	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R2275:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R3004:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21,581,771 (GRCm39)	missense	possibly damaging	0.68
R3943:Gpr158	UTSW	2	21,373,370 (GRCm39)	missense	possibly damaging	0.65
R4238:Gpr158	UTSW	2	21,373,362 (GRCm39)	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21,830,025 (GRCm39)	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21,832,403 (GRCm39)	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4506:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21,373,811 (GRCm39)	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21,831,864 (GRCm39)	missense	probably benign
R4798:Gpr158	UTSW	2	21,787,993 (GRCm39)	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21,830,059 (GRCm39)	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21,831,968 (GRCm39)	missense	probably damaging	1.00
R5334:Gpr158	UTSW	2	21,832,316 (GRCm39)	missense	probably benign	0.01
R5560:Gpr158	UTSW	2	21,831,101 (GRCm39)	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21,832,046 (GRCm39)	missense	probably benign
R5637:Gpr158	UTSW	2	21,788,083 (GRCm39)	missense	probably benign	0.00
R5701:Gpr158	UTSW	2	21,751,520 (GRCm39)	missense	probably damaging	1.00
R5744:Gpr158	UTSW	2	21,373,331 (GRCm39)	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21,373,932 (GRCm39)	missense	possibly damaging	0.95
R6200:Gpr158	UTSW	2	21,404,227 (GRCm39)	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21,820,422 (GRCm39)	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21,815,365 (GRCm39)	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21,831,099 (GRCm39)	missense	probably damaging	1.00
R6698:Gpr158	UTSW	2	21,831,921 (GRCm39)	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21,653,802 (GRCm39)	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21,831,386 (GRCm39)	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21,373,113 (GRCm39)	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21,815,412 (GRCm39)	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21,581,750 (GRCm39)	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R7515:Gpr158	UTSW	2	21,373,092 (GRCm39)	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21,831,158 (GRCm39)	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R8311:Gpr158	UTSW	2	21,373,701 (GRCm39)	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21,581,693 (GRCm39)	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21,404,149 (GRCm39)	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21,558,137 (GRCm39)	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21,581,751 (GRCm39)	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21,581,760 (GRCm39)	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21,830,078 (GRCm39)	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21,831,251 (GRCm39)	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9317:Gpr158	UTSW	2	21,832,037 (GRCm39)	missense	probably benign
R9379:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9428:Gpr158	UTSW	2	21,787,972 (GRCm39)	missense	probably benign
R9497:Gpr158	UTSW	2	21,831,825 (GRCm39)	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21,830,054 (GRCm39)	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21,831,315 (GRCm39)	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21,831,180 (GRCm39)	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21,815,501 (GRCm39)	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21,832,083 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- TGCTCGCTCATCTGGGATTG -3'
(R):5'- GTCTGTTCCCGAGACTTATTGC -3'

Sequencing Primer
(F):5'- CGCTCATCTGGGATTGGGAGAG -3'
(R):5'- CCCGAGACTTATTGCTCTGC -3'

Posted On

2017-06-26