Mutagenetix > Incidental Mutation

Incidental Mutation 'R6082:Clec4b2'

482234

Institutional Source

Beutler Lab

Gene Symbol

Clec4b2

Ensembl Gene

ENSMUSG00000067767

Gene Name

C-type lectin domain family 4, member b2

Synonyms

mDCAR1, F830043G12Rik

MMRRC Submission

044241-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R6082 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

123149852-123181630 bp(+) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

A to T at 123181100 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000085802 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088455] [ENSMUST00000088455]

AlphaFold

Q67DU8

Predicted Effect

probably null
Transcript: ENSMUST00000088455

SMART Domains

Protein: ENSMUSP00000085802
Gene: ENSMUSG00000067767

Domain	Start	End	E-Value	Type
transmembrane domain	21	43	N/A	INTRINSIC
CLECT	79	202	1.87e-33	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000088455

SMART Domains

Protein: ENSMUSP00000085802
Gene: ENSMUSG00000067767

Domain	Start	End	E-Value	Type
transmembrane domain	21	43	N/A	INTRINSIC
CLECT	79	202	1.87e-33	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	T	C	1: 11,868,752 (GRCm39)	I344T	probably benign	Het
Adamts19	G	A	18: 59,101,846 (GRCm39)	A639T	probably benign	Het
Adamts9	T	C	6: 92,866,930 (GRCm39)	D122G	probably damaging	Het
Bin2	A	G	15: 100,543,029 (GRCm39)	S358P	possibly damaging	Het
Bora	A	T	14: 99,299,730 (GRCm39)	Q234L	possibly damaging	Het
Btaf1	C	T	19: 36,960,942 (GRCm39)	R772C	probably damaging	Het
Cenpf	T	C	1: 189,390,301 (GRCm39)	E1177G	probably benign	Het
Dedd2	G	A	7: 24,910,715 (GRCm39)	P154S	probably benign	Het
Dennd5b	A	G	6: 148,970,193 (GRCm39)	S87P	probably damaging	Het
Dhx8	C	T	11: 101,655,139 (GRCm39)	R1050W	probably damaging	Het
Eif3a	T	C	19: 60,760,568 (GRCm39)	K682R	possibly damaging	Het
Eif4e2	G	T	1: 87,153,956 (GRCm39)		probably null	Het
Ephb1	T	C	9: 101,848,303 (GRCm39)	D665G	probably damaging	Het
Gbp7	G	A	3: 142,251,697 (GRCm39)	V515M	probably benign	Het
Hnrnpdl	C	T	5: 100,184,340 (GRCm39)	G398S	probably null	Het
Hook3	C	A	8: 26,600,813 (GRCm39)	A32S	probably benign	Het
Hoxb4	T	C	11: 96,209,359 (GRCm39)		probably benign	Het
Idi1	T	A	13: 8,940,506 (GRCm39)	Y229*	probably null	Het
Lpl	A	T	8: 69,349,301 (GRCm39)	I276F	probably damaging	Het
Ltbp4	T	C	7: 27,035,105 (GRCm39)		probably benign	Het
Mphosph8	A	G	14: 56,905,998 (GRCm39)	I64V	probably damaging	Het
Mroh1	A	G	15: 76,314,423 (GRCm39)	D700G	probably benign	Het
Myt1l	G	A	12: 29,882,331 (GRCm39)	G509R	unknown	Het
Myt1l	T	C	12: 29,892,518 (GRCm39)	Y52H	probably damaging	Het
Nlrp9a	T	A	7: 26,267,402 (GRCm39)	D777E	probably benign	Het
Nudt4	A	G	10: 95,387,318 (GRCm39)	I82T	probably benign	Het
Nwd2	T	C	5: 63,962,374 (GRCm39)	C653R	possibly damaging	Het
Obscn	A	G	11: 58,890,393 (GRCm39)	Y7380H	unknown	Het
Or13a18	A	G	7: 140,190,594 (GRCm39)	T172A	probably benign	Het
Or8b3b	C	A	9: 38,583,866 (GRCm39)	R291S	probably damaging	Het
Or8j3	A	C	2: 86,028,661 (GRCm39)	V145G	probably damaging	Het
Or8s8	C	T	15: 98,354,647 (GRCm39)	A152V	probably damaging	Het
Ptk2	A	T	15: 73,148,714 (GRCm39)	M409K	probably damaging	Het
Ptpn11	T	C	5: 121,292,589 (GRCm39)	T253A	probably benign	Het
Rabl6	C	T	2: 25,473,837 (GRCm39)		probably benign	Het
Rnf14	T	C	18: 38,434,723 (GRCm39)	S57P	possibly damaging	Het
Scnm1	T	C	3: 95,037,596 (GRCm39)	I157V	probably benign	Het
Slco1b2	A	C	6: 141,609,396 (GRCm39)	I269L	probably benign	Het
Smchd1	A	G	17: 71,656,714 (GRCm39)	Y1918H	probably benign	Het
Spag17	A	T	3: 100,031,501 (GRCm39)	I2255F	possibly damaging	Het
Spata31g1	A	G	4: 42,972,511 (GRCm39)	M615V	probably benign	Het
Tnnt2	A	G	1: 135,777,172 (GRCm39)	M199V	probably benign	Het
Tymp	GC	GCC	15: 89,258,567 (GRCm39)		probably null	Het
Vmn2r73	T	A	7: 85,507,429 (GRCm39)	I628L	probably benign	Het
Zc2hc1b	C	A	10: 13,047,055 (GRCm39)	E19*	probably null	Het

Other mutations in Clec4b2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00956:Clec4b2	APN	6	123,179,110 (GRCm39)	nonsense	probably null
IGL01753:Clec4b2	APN	6	123,179,169 (GRCm39)	missense	possibly damaging	0.90
IGL02168:Clec4b2	APN	6	123,181,156 (GRCm39)	missense	probably damaging	0.98
IGL02388:Clec4b2	APN	6	123,179,187 (GRCm39)	splice site	probably null
IGL03194:Clec4b2	APN	6	123,177,946 (GRCm39)	missense	probably benign	0.07
P0041:Clec4b2	UTSW	6	123,158,253 (GRCm39)	missense	possibly damaging	0.72
R0013:Clec4b2	UTSW	6	123,179,108 (GRCm39)	missense	probably damaging	1.00
R0121:Clec4b2	UTSW	6	123,181,131 (GRCm39)	missense	probably benign	0.02
R0401:Clec4b2	UTSW	6	123,158,259 (GRCm39)	nonsense	probably null
R1072:Clec4b2	UTSW	6	123,181,233 (GRCm39)	missense	probably damaging	0.99
R2520:Clec4b2	UTSW	6	123,177,942 (GRCm39)	missense	probably damaging	1.00
R4575:Clec4b2	UTSW	6	123,150,639 (GRCm39)	missense	probably damaging	0.99
R4897:Clec4b2	UTSW	6	123,177,999 (GRCm39)	nonsense	probably null
R4898:Clec4b2	UTSW	6	123,181,163 (GRCm39)	missense	probably benign	0.36
R5022:Clec4b2	UTSW	6	123,177,915 (GRCm39)	missense	probably null	1.00
R5023:Clec4b2	UTSW	6	123,177,915 (GRCm39)	missense	probably null	1.00
R5057:Clec4b2	UTSW	6	123,177,915 (GRCm39)	missense	probably null	1.00
R5404:Clec4b2	UTSW	6	123,158,308 (GRCm39)	missense	probably benign	0.06
R5508:Clec4b2	UTSW	6	123,150,001 (GRCm39)	start gained	probably benign
R6333:Clec4b2	UTSW	6	123,177,637 (GRCm39)	splice site	probably null
R6902:Clec4b2	UTSW	6	123,177,987 (GRCm39)	nonsense	probably null
R6946:Clec4b2	UTSW	6	123,177,987 (GRCm39)	nonsense	probably null
R7144:Clec4b2	UTSW	6	123,158,343 (GRCm39)	missense	probably benign	0.02
R7709:Clec4b2	UTSW	6	123,149,974 (GRCm39)	start gained	probably benign
R7973:Clec4b2	UTSW	6	123,181,148 (GRCm39)	missense	probably benign	0.05
R8810:Clec4b2	UTSW	6	123,158,269 (GRCm39)	missense	probably benign	0.23
R9278:Clec4b2	UTSW	6	123,181,224 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCAATGGGTTGAATGTCCCC -3'
(R):5'- TGCAATCCCAAGGCCATAAAGG -3'

Sequencing Primer
(F):5'- GTTGAATGTCCCCTGGTACCAAAC -3'
(R):5'- CCAAGGCCATAAAGGATATGTGATTC -3'

Posted On

2017-07-14