Mutagenetix > Incidental Mutation

Incidental Mutation 'R6074:Wnk2'

482661

Institutional Source

Beutler Lab

Gene Symbol

Wnk2

Ensembl Gene

ENSMUSG00000037989

Gene Name

WNK lysine deficient protein kinase 2

Synonyms

X83337, ESTM15, 1810073P09Rik

MMRRC Submission

044235-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.287) question?

Stock #

R6074 (G1)

Quality Score

163.009

Status

Validated

Chromosome

Chromosomal Location

49189779-49301490 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 49204875 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 458 (C458S)

Ref Sequence

ENSEMBL: ENSMUSP00000124598 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035538] [ENSMUST00000049265] [ENSMUST00000091623] [ENSMUST00000110096] [ENSMUST00000110097] [ENSMUST00000159196] [ENSMUST00000159559] [ENSMUST00000160087] [ENSMUST00000162403] [ENSMUST00000162581]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000035538

SMART Domains

Protein: ENSMUSP00000047231
Gene: ENSMUSG00000037989

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000049265
AA Change: C1963S

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000049327
Gene: ENSMUSG00000037989
AA Change: C1963S

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
S_TKc	195	453	3.3e-19	SMART
Pfam:OSR1_C	474	511	5.6e-19	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	9.51e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1703	2.12e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2023	2.12e-5	PROSPERO
low complexity region	2069	2089	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000091623
AA Change: C1963S

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000089212
Gene: ENSMUSG00000037989
AA Change: C1963S

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	1.6e-41	PFAM
Pfam:Pkinase	195	453	1e-54	PFAM
Pfam:OSR1_C	474	511	4e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.52e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	7.41e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	7.41e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC
low complexity region	2191	2202	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110096
AA Change: C1864S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000105723
Gene: ENSMUSG00000037989
AA Change: C1864S

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.6e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	1015	1038	N/A	INTRINSIC
internal_repeat_1	1064	1087	2.89e-5	PROSPERO
low complexity region	1177	1194	N/A	INTRINSIC
low complexity region	1219	1255	N/A	INTRINSIC
low complexity region	1331	1343	N/A	INTRINSIC
low complexity region	1351	1359	N/A	INTRINSIC
low complexity region	1366	1391	N/A	INTRINSIC
low complexity region	1489	1498	N/A	INTRINSIC
low complexity region	1721	1732	N/A	INTRINSIC
coiled coil region	1737	1768	N/A	INTRINSIC
low complexity region	2018	2038	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110097
AA Change: C1963S

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000105724
Gene: ENSMUSG00000037989
AA Change: C1963S

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.16e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	5.74e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	5.74e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000159196
AA Change: C458S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000124598
Gene: ENSMUSG00000037989
AA Change: C458S

Domain	Start	End	E-Value	Type
low complexity region	82	92	N/A	INTRINSIC
low complexity region	315	326	N/A	INTRINSIC
coiled coil region	331	362	N/A	INTRINSIC
low complexity region	612	632	N/A	INTRINSIC
low complexity region	686	697	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000159559
AA Change: C1951S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000123915
Gene: ENSMUSG00000037989
AA Change: C1951S

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	971	1002	N/A	INTRINSIC
low complexity region	1009	1036	N/A	INTRINSIC
low complexity region	1101	1124	N/A	INTRINSIC
internal_repeat_1	1150	1173	7.19e-6	PROSPERO
low complexity region	1263	1280	N/A	INTRINSIC
low complexity region	1305	1341	N/A	INTRINSIC
low complexity region	1417	1429	N/A	INTRINSIC
low complexity region	1437	1445	N/A	INTRINSIC
low complexity region	1452	1477	N/A	INTRINSIC
low complexity region	1575	1585	N/A	INTRINSIC
internal_repeat_2	1599	1701	3.66e-5	PROSPERO
low complexity region	1808	1819	N/A	INTRINSIC
coiled coil region	1824	1855	N/A	INTRINSIC
internal_repeat_2	1911	2015	3.66e-5	PROSPERO
low complexity region	2105	2125	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000160087
AA Change: C1104S

PolyPhen 2 Score 0.969 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000124614
Gene: ENSMUSG00000037989
AA Change: C1104S

Domain	Start	End	E-Value	Type
low complexity region	61	71	N/A	INTRINSIC
low complexity region	80	94	N/A	INTRINSIC
low complexity region	115	130	N/A	INTRINSIC
low complexity region	178	194	N/A	INTRINSIC
low complexity region	255	278	N/A	INTRINSIC
low complexity region	417	434	N/A	INTRINSIC
low complexity region	459	495	N/A	INTRINSIC
low complexity region	571	583	N/A	INTRINSIC
low complexity region	591	599	N/A	INTRINSIC
low complexity region	606	631	N/A	INTRINSIC
low complexity region	729	738	N/A	INTRINSIC
low complexity region	961	972	N/A	INTRINSIC
coiled coil region	977	1008	N/A	INTRINSIC
low complexity region	1258	1278	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000162403
AA Change: C1853S

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000125539
Gene: ENSMUSG00000037989
AA Change: C1853S

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.3e-42	PFAM
Pfam:Pkinase	195	453	1.6e-56	PFAM
Pfam:OSR1_C	474	511	5.3e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	1003	1026	N/A	INTRINSIC
internal_repeat_1	1052	1075	1.05e-5	PROSPERO
low complexity region	1165	1182	N/A	INTRINSIC
low complexity region	1207	1243	N/A	INTRINSIC
low complexity region	1319	1331	N/A	INTRINSIC
low complexity region	1339	1347	N/A	INTRINSIC
low complexity region	1354	1379	N/A	INTRINSIC
low complexity region	1477	1487	N/A	INTRINSIC
internal_repeat_2	1501	1593	2.32e-5	PROSPERO
low complexity region	1710	1721	N/A	INTRINSIC
coiled coil region	1726	1757	N/A	INTRINSIC
internal_repeat_2	1813	1913	2.32e-5	PROSPERO
low complexity region	1959	1979	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162581

SMART Domains

Protein: ENSMUSP00000125448
Gene: ENSMUSG00000037989

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Meta Mutation Damage Score

0.1713

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.1%

Validation Efficiency

97% (61/63)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Amigo1	T	A	3: 108,099,009 (GRCm39)	C189*	probably null	Het
Auts2	G	T	5: 131,505,828 (GRCm39)		probably benign	Het
Cadps2	C	A	6: 23,626,670 (GRCm39)	W177L	probably damaging	Het
Camk2b	G	T	11: 5,939,635 (GRCm39)	P223Q	probably damaging	Het
Ccdc74a	C	A	16: 17,464,591 (GRCm39)	S9*	probably null	Het
Ccnt1	A	C	15: 98,441,205 (GRCm39)	Y688D	probably damaging	Het
Cdhr1	T	C	14: 36,801,600 (GRCm39)	N781S	probably benign	Het
Ceacam3	C	T	7: 16,885,484 (GRCm39)	T29I	probably benign	Het
Cep170b	T	C	12: 112,710,589 (GRCm39)	S1473P	probably damaging	Het
Col19a1	T	A	1: 24,565,564 (GRCm39)	T256S	unknown	Het
Eif2d	T	A	1: 131,094,079 (GRCm39)	D423E	probably damaging	Het
Fam13a	A	G	6: 58,966,723 (GRCm39)		probably null	Het
Gaa	G	A	11: 119,175,012 (GRCm39)	R871H	probably benign	Het
Galnt12	T	A	4: 47,112,405 (GRCm39)	V37D	probably damaging	Het
Glp2r	A	G	11: 67,637,640 (GRCm39)	S13P	unknown	Het
Gnptab	C	T	10: 88,268,940 (GRCm39)	L548F	probably damaging	Het
Heg1	C	T	16: 33,547,573 (GRCm39)	P787S	possibly damaging	Het
Hivep2	T	A	10: 14,007,485 (GRCm39)	M1361K	probably benign	Het
Hivep3	T	C	4: 119,954,891 (GRCm39)	I1069T	possibly damaging	Het
Hspg2	A	G	4: 137,268,046 (GRCm39)	D2121G	probably benign	Het
Htatip2	A	G	7: 49,422,322 (GRCm39)		probably null	Het
Ighv1-75	T	C	12: 115,798,007 (GRCm39)	S4G	probably benign	Het
Ip6k1	A	G	9: 107,901,308 (GRCm39)		probably benign	Het
Kcna6	A	G	6: 126,716,242 (GRCm39)	S216P	probably benign	Het
Kif21a	T	A	15: 90,865,095 (GRCm39)	T526S	probably benign	Het
Kng2	C	T	16: 22,819,346 (GRCm39)	G230D	probably benign	Het
Lgi2	A	T	5: 52,703,984 (GRCm39)	F216I	probably benign	Het
Lratd1	G	A	12: 14,200,512 (GRCm39)	H72Y	probably benign	Het
Lss	T	A	10: 76,379,693 (GRCm39)	N383K	probably damaging	Het
Mical1	T	C	10: 41,362,061 (GRCm39)	M929T	probably benign	Het
Mroh2a	A	G	1: 88,186,386 (GRCm39)	S64G	probably benign	Het
Nat8f4	A	G	6: 85,878,169 (GRCm39)	V118A	probably damaging	Het
Or10j5	T	A	1: 172,784,945 (GRCm39)	N194K	probably benign	Het
Or4p23	A	C	2: 88,576,566 (GRCm39)	L222R	probably damaging	Het
Or6b2	G	A	1: 92,407,497 (GRCm39)	T282M	probably benign	Het
Osbpl11	T	A	16: 33,030,335 (GRCm39)	S188T	probably benign	Het
Pea15a	C	T	1: 172,026,752 (GRCm39)	R85H	possibly damaging	Het
Ppp1r13b	A	G	12: 111,798,836 (GRCm39)	I919T	probably damaging	Het
Rasgrf1	A	G	9: 89,835,968 (GRCm39)	D232G	probably benign	Het
Rp1	A	G	1: 4,415,602 (GRCm39)	S1837P	probably benign	Het
Samd5	T	A	10: 9,550,334 (GRCm39)	Y125F	possibly damaging	Het
Skint5	T	C	4: 113,662,397 (GRCm39)	T597A	unknown	Het
Slc25a12	A	G	2: 71,106,798 (GRCm39)	F605L	probably benign	Het
Slc39a12	C	T	2: 14,412,290 (GRCm39)	Q321*	probably null	Het
Slc6a19	T	C	13: 73,837,882 (GRCm39)	S206G	probably benign	Het
Tacc2	T	A	7: 130,227,165 (GRCm39)	H1283Q	possibly damaging	Het
Thumpd3	A	G	6: 113,036,971 (GRCm39)	E290G	possibly damaging	Het
Timd2	C	T	11: 46,577,999 (GRCm39)	G44D	possibly damaging	Het
Tmem74	A	G	15: 43,730,134 (GRCm39)	V303A	possibly damaging	Het
Tpo	A	G	12: 30,128,186 (GRCm39)	V785A	probably benign	Het
Tpra1	T	A	6: 88,888,919 (GRCm39)	I367N	possibly damaging	Het
Txnrd2	T	C	16: 18,256,297 (GRCm39)	I95T	probably damaging	Het
Usp32	A	G	11: 84,885,399 (GRCm39)	V1216A	probably benign	Het
Usp54	C	A	14: 20,602,167 (GRCm39)	M1394I	probably benign	Het
Vmn2r13	A	G	5: 109,322,167 (GRCm39)	F177L	probably benign	Het
Vmn2r82	T	A	10: 79,232,377 (GRCm39)	V792E	probably damaging	Het
Wdfy4	C	T	14: 32,805,596 (GRCm39)		probably null	Het
Xkr6	T	G	14: 63,844,519 (GRCm39)	F181V	possibly damaging	Het
Zfp212	T	A	6: 47,903,986 (GRCm39)	C149*	probably null	Het
Zfp647	G	A	15: 76,796,285 (GRCm39)	P125L	probably damaging	Het
Zfp661	A	G	2: 127,419,793 (GRCm39)	S116P	probably benign	Het
Zfp770	A	G	2: 114,026,870 (GRCm39)	Y400H	possibly damaging	Het

Other mutations in Wnk2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Wnk2	APN	13	49,221,668 (GRCm39)	missense	possibly damaging	0.89
IGL01575:Wnk2	APN	13	49,300,152 (GRCm39)	missense	probably damaging	1.00
IGL01601:Wnk2	APN	13	49,230,038 (GRCm39)	missense	probably damaging	1.00
IGL01775:Wnk2	APN	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
IGL02013:Wnk2	APN	13	49,235,510 (GRCm39)	missense	possibly damaging	0.46
IGL02016:Wnk2	APN	13	49,210,381 (GRCm39)	missense	probably damaging	1.00
IGL02167:Wnk2	APN	13	49,224,601 (GRCm39)	critical splice acceptor site	probably null
IGL02174:Wnk2	APN	13	49,210,643 (GRCm39)	missense	probably damaging	1.00
IGL02210:Wnk2	APN	13	49,244,345 (GRCm39)	missense	probably damaging	0.98
IGL02228:Wnk2	APN	13	49,210,416 (GRCm39)	missense	probably damaging	1.00
IGL02282:Wnk2	APN	13	49,221,601 (GRCm39)	missense	probably damaging	1.00
IGL02319:Wnk2	APN	13	49,214,914 (GRCm39)	missense	possibly damaging	0.73
IGL02394:Wnk2	APN	13	49,235,375 (GRCm39)	splice site	probably null
IGL02624:Wnk2	APN	13	49,256,278 (GRCm39)	missense	probably damaging	1.00
IGL02743:Wnk2	APN	13	49,248,920 (GRCm39)	missense	probably damaging	1.00
IGL03012:Wnk2	APN	13	49,197,865 (GRCm39)	missense	probably damaging	0.99
IGL03166:Wnk2	APN	13	49,224,520 (GRCm39)	nonsense	probably null
R0034:Wnk2	UTSW	13	49,221,556 (GRCm39)	missense	possibly damaging	0.64
R0385:Wnk2	UTSW	13	49,221,604 (GRCm39)	missense	probably damaging	1.00
R0423:Wnk2	UTSW	13	49,248,894 (GRCm39)	missense	possibly damaging	0.91
R0504:Wnk2	UTSW	13	49,238,872 (GRCm39)	missense	probably damaging	1.00
R0504:Wnk2	UTSW	13	49,238,870 (GRCm39)	missense	possibly damaging	0.92
R0653:Wnk2	UTSW	13	49,210,492 (GRCm39)	missense	possibly damaging	0.85
R1135:Wnk2	UTSW	13	49,230,034 (GRCm39)	missense	probably damaging	1.00
R1445:Wnk2	UTSW	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1480:Wnk2	UTSW	13	49,210,708 (GRCm39)	missense	probably damaging	1.00
R1605:Wnk2	UTSW	13	49,214,370 (GRCm39)	missense	probably damaging	1.00
R1719:Wnk2	UTSW	13	49,214,202 (GRCm39)	missense	possibly damaging	0.76
R1891:Wnk2	UTSW	13	49,206,200 (GRCm39)	nonsense	probably null
R1966:Wnk2	UTSW	13	49,192,487 (GRCm39)	missense	probably damaging	0.96
R2001:Wnk2	UTSW	13	49,232,158 (GRCm39)	missense	possibly damaging	0.61
R2310:Wnk2	UTSW	13	49,204,053 (GRCm39)	missense	probably damaging	0.97
R2356:Wnk2	UTSW	13	49,192,644 (GRCm39)	nonsense	probably null
R2406:Wnk2	UTSW	13	49,214,964 (GRCm39)	missense	possibly damaging	0.86
R2519:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably damaging	0.99
R3962:Wnk2	UTSW	13	49,224,453 (GRCm39)	missense	probably damaging	1.00
R4160:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4161:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4226:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4227:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4234:Wnk2	UTSW	13	49,214,604 (GRCm39)	missense	probably benign	0.33
R4304:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4308:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4584:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4655:Wnk2	UTSW	13	49,210,359 (GRCm39)	missense	probably damaging	1.00
R4715:Wnk2	UTSW	13	49,300,708 (GRCm39)	start codon destroyed	unknown
R4887:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4888:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4945:Wnk2	UTSW	13	49,210,722 (GRCm39)	missense	probably damaging	1.00
R5182:Wnk2	UTSW	13	49,214,637 (GRCm39)	missense	possibly damaging	0.92
R5243:Wnk2	UTSW	13	49,226,054 (GRCm39)	missense	possibly damaging	0.51
R5370:Wnk2	UTSW	13	49,256,437 (GRCm39)	missense	probably damaging	1.00
R5771:Wnk2	UTSW	13	49,256,276 (GRCm39)	missense	probably damaging	1.00
R5877:Wnk2	UTSW	13	49,220,782 (GRCm39)	missense	probably damaging	0.98
R5900:Wnk2	UTSW	13	49,256,308 (GRCm39)	missense	probably damaging	1.00
R5905:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R5912:Wnk2	UTSW	13	49,214,250 (GRCm39)	missense	probably damaging	1.00
R5915:Wnk2	UTSW	13	49,231,561 (GRCm39)	missense	probably damaging	0.99
R6028:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R6171:Wnk2	UTSW	13	49,214,308 (GRCm39)	missense	probably damaging	1.00
R6368:Wnk2	UTSW	13	49,214,814 (GRCm39)	missense	probably damaging	0.99
R6467:Wnk2	UTSW	13	49,300,081 (GRCm39)	missense	probably damaging	1.00
R6501:Wnk2	UTSW	13	49,300,159 (GRCm39)	missense	probably damaging	1.00
R6849:Wnk2	UTSW	13	49,220,834 (GRCm39)	missense	probably damaging	1.00
R6898:Wnk2	UTSW	13	49,224,557 (GRCm39)	missense	probably damaging	1.00
R6949:Wnk2	UTSW	13	49,254,616 (GRCm39)	missense	probably damaging	1.00
R7011:Wnk2	UTSW	13	49,224,567 (GRCm39)	missense	probably damaging	0.99
R7097:Wnk2	UTSW	13	49,256,314 (GRCm39)	missense	possibly damaging	0.86
R7121:Wnk2	UTSW	13	49,300,653 (GRCm39)	missense	probably benign	0.26
R7123:Wnk2	UTSW	13	49,235,462 (GRCm39)	missense	possibly damaging	0.90
R7423:Wnk2	UTSW	13	49,191,608 (GRCm39)	missense	probably benign	0.07
R7502:Wnk2	UTSW	13	49,300,720 (GRCm39)	splice site	probably null
R7529:Wnk2	UTSW	13	49,254,457 (GRCm39)	missense	possibly damaging	0.50
R7751:Wnk2	UTSW	13	49,231,493 (GRCm39)	missense	unknown
R7979:Wnk2	UTSW	13	49,248,884 (GRCm39)	missense	probably damaging	1.00
R8118:Wnk2	UTSW	13	49,244,459 (GRCm39)	missense	probably damaging	0.99
R8121:Wnk2	UTSW	13	49,214,415 (GRCm39)	nonsense	probably null
R8155:Wnk2	UTSW	13	49,192,577 (GRCm39)	missense	unknown
R8329:Wnk2	UTSW	13	49,248,914 (GRCm39)	missense	probably damaging	1.00
R8334:Wnk2	UTSW	13	49,203,958 (GRCm39)	critical splice donor site	probably null
R8872:Wnk2	UTSW	13	49,210,960 (GRCm39)	missense	probably benign	0.00
R8919:Wnk2	UTSW	13	49,221,711 (GRCm39)	missense	possibly damaging	0.86
R9091:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9234:Wnk2	UTSW	13	49,224,274 (GRCm39)	missense	probably damaging	0.99
R9262:Wnk2	UTSW	13	49,221,430 (GRCm39)	missense	probably benign	0.12
R9268:Wnk2	UTSW	13	49,235,507 (GRCm39)	missense	possibly damaging	0.82
R9270:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9386:Wnk2	UTSW	13	49,220,822 (GRCm39)	missense	probably damaging	0.98
R9582:Wnk2	UTSW	13	49,210,975 (GRCm39)	missense	probably benign	0.01
R9617:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
R9625:Wnk2	UTSW	13	49,254,445 (GRCm39)	missense	probably benign	0.20
R9794:Wnk2	UTSW	13	49,229,674 (GRCm39)	missense	probably benign	0.02
RF023:Wnk2	UTSW	13	49,300,255 (GRCm39)	missense	probably benign	0.00
X0025:Wnk2	UTSW	13	49,214,418 (GRCm39)	missense	probably damaging	0.99
X0063:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
Z1176:Wnk2	UTSW	13	49,191,537 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GCGGGACAATTCCATTCTTTG -3'
(R):5'- AGATTCAACTTCCCCAAGGTC -3'

Posted On

2017-07-14