Incidental Mutation 'R6081:Speer4a'
ID482915
Institutional Source Beutler Lab
Gene Symbol Speer4a
Ensembl Gene ENSMUSG00000073119
Gene Namespermatogenesis associated glutamate (E)-rich protein 4A
Synonyms1700027N01Rik, SPEER-4A
MMRRC Submission 044240-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.053) question?
Stock #R6081 (G1)
Quality Score121.008
Status Not validated
Chromosome5
Chromosomal Location26032846-26039558 bp(-) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) A to T at 26034962 bp
ZygosityHeterozygous
Amino Acid Change Cysteine to Stop codon at position 263 (C263*)
Ref Sequence ENSEMBL: ENSMUSP00000078415 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000079447]
Predicted Effect probably null
Transcript: ENSMUST00000079447
AA Change: C263*
SMART Domains Protein: ENSMUSP00000078415
Gene: ENSMUSG00000073119
AA Change: C263*

DomainStartEndE-ValueType
Pfam:Takusan 50 134 1.7e-26 PFAM
low complexity region 135 146 N/A INTRINSIC
low complexity region 154 168 N/A INTRINSIC
coiled coil region 183 223 N/A INTRINSIC
low complexity region 248 261 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 97.9%
  • 20x: 93.8%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 29 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930522L14Rik A T 5: 109,739,231 M33K probably damaging Het
4930556J24Rik T C 11: 3,938,140 Q82R unknown Het
Adcy9 T C 16: 4,294,681 D714G probably benign Het
Afg3l2 G T 18: 67,421,259 L458M probably damaging Het
Ahctf1 G T 1: 179,781,672 A639E probably benign Het
Ank3 G A 10: 70,002,565 R1566K possibly damaging Het
Anxa5 C T 3: 36,465,287 D18N probably damaging Het
Apc C T 18: 34,290,111 P299L possibly damaging Het
Btnl4 C A 17: 34,474,236 W68C probably damaging Het
Cmya5 T A 13: 93,144,513 probably benign Het
Cstf2t G T 19: 31,083,123 V20L probably benign Het
D630045J12Rik A T 6: 38,142,698 V1703E probably damaging Het
Dhx32 A G 7: 133,722,212 F535S probably damaging Het
Diras2 T C 13: 52,508,145 D42G probably damaging Het
Dppa3 T A 6: 122,629,972 D140E probably damaging Het
Gpr180 C T 14: 118,153,674 T205I probably benign Het
Gzme T G 14: 56,118,307 T183P possibly damaging Het
H2-T23 T A 17: 36,031,815 I144F possibly damaging Het
Hlx T C 1: 184,727,697 S415G probably benign Het
Krtap5-3 G A 7: 142,201,486 C20Y unknown Het
Mcm8 G T 2: 132,828,083 R359L probably benign Het
Mroh2b G C 15: 4,944,377 E1126Q probably damaging Het
Nav1 C T 1: 135,470,822 R674H probably damaging Het
Otx1 A T 11: 21,999,406 L24H probably damaging Het
Rnaset2b T A 17: 6,988,794 probably null Het
Samd12 T C 15: 53,719,678 K87E probably benign Het
Sec16b T C 1: 157,560,754 S564P probably benign Het
Susd1 C A 4: 59,411,359 C158F possibly damaging Het
Vmn2r6 C T 3: 64,556,532 V294M probably benign Het
Other mutations in Speer4a
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01783:Speer4a APN 5 26035047 missense possibly damaging 0.95
IGL01959:Speer4a APN 5 26035904 nonsense probably null
IGL02294:Speer4a APN 5 26038242 missense probably benign 0.21
FR4340:Speer4a UTSW 5 26036748 nonsense probably null
FR4342:Speer4a UTSW 5 26036748 nonsense probably null
FR4589:Speer4a UTSW 5 26036748 nonsense probably null
R0137:Speer4a UTSW 5 26035984 missense possibly damaging 0.95
R1068:Speer4a UTSW 5 26036026 missense probably null 0.84
R1209:Speer4a UTSW 5 26035125 critical splice acceptor site probably null
R3805:Speer4a UTSW 5 26035084 missense possibly damaging 0.76
R4525:Speer4a UTSW 5 26039343 critical splice donor site probably null
R4851:Speer4a UTSW 5 26038212 missense probably damaging 0.99
R5326:Speer4a UTSW 5 26036738 missense probably damaging 0.99
R5542:Speer4a UTSW 5 26036738 missense probably damaging 0.99
R6742:Speer4a UTSW 5 26036056 splice site probably null
R7521:Speer4a UTSW 5 26036765 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TTGTCTCAAGAGTCACTACTTGC -3'
(R):5'- TTCCTCTCCCAGGTCTCAGAAG -3'

Sequencing Primer
(F):5'- GTCTCAAGAGTCACTACTTGCAAATC -3'
(R):5'- CCAGGTCTCAGAAGCTGCAAG -3'
Posted On2017-07-14