Incidental Mutation 'R6081:Gpr180'
Institutional Source Beutler Lab
Gene Symbol Gpr180
Ensembl Gene ENSMUSG00000022131
Gene NameG protein-coupled receptor 180
SynonymsITR, E130016I23Rik
MMRRC Submission 044240-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.198) question?
Stock #R6081 (G1)
Quality Score225.009
Status Not validated
Chromosomal Location118137158-118163261 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to T at 118153674 bp
Amino Acid Change Threonine to Isoleucine at position 205 (T205I)
Ref Sequence ENSEMBL: ENSMUSP00000022728 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000022728]
Predicted Effect probably benign
Transcript: ENSMUST00000022728
AA Change: T205I

PolyPhen 2 Score 0.022 (Sensitivity: 0.95; Specificity: 0.81)
SMART Domains Protein: ENSMUSP00000022728
Gene: ENSMUSG00000022131
AA Change: T205I

signal peptide 1 23 N/A INTRINSIC
Pfam:Lung_7-TM_R 132 418 2.1e-12 PFAM
Pfam:GpcrRhopsn4 142 406 6.1e-88 PFAM
low complexity region 419 433 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 97.9%
  • 20x: 93.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that is a member of the G protein-coupled receptor superfamily. This protein is produced predominantly in vascular smooth muscle cells and may play an important role in the regulation of vascular remodeling. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele are resistant to cuff-induced intimal thickening of the femoral artery. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 29 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930522L14Rik A T 5: 109,739,231 M33K probably damaging Het
4930556J24Rik T C 11: 3,938,140 Q82R unknown Het
Adcy9 T C 16: 4,294,681 D714G probably benign Het
Afg3l2 G T 18: 67,421,259 L458M probably damaging Het
Ahctf1 G T 1: 179,781,672 A639E probably benign Het
Ank3 G A 10: 70,002,565 R1566K possibly damaging Het
Anxa5 C T 3: 36,465,287 D18N probably damaging Het
Apc C T 18: 34,290,111 P299L possibly damaging Het
Btnl4 C A 17: 34,474,236 W68C probably damaging Het
Cmya5 T A 13: 93,144,513 probably benign Het
Cstf2t G T 19: 31,083,123 V20L probably benign Het
D630045J12Rik A T 6: 38,142,698 V1703E probably damaging Het
Dhx32 A G 7: 133,722,212 F535S probably damaging Het
Diras2 T C 13: 52,508,145 D42G probably damaging Het
Dppa3 T A 6: 122,629,972 D140E probably damaging Het
Gzme T G 14: 56,118,307 T183P possibly damaging Het
H2-T23 T A 17: 36,031,815 I144F possibly damaging Het
Hlx T C 1: 184,727,697 S415G probably benign Het
Krtap5-3 G A 7: 142,201,486 C20Y unknown Het
Mcm8 G T 2: 132,828,083 R359L probably benign Het
Mroh2b G C 15: 4,944,377 E1126Q probably damaging Het
Nav1 C T 1: 135,470,822 R674H probably damaging Het
Otx1 A T 11: 21,999,406 L24H probably damaging Het
Rnaset2b T A 17: 6,988,794 probably null Het
Samd12 T C 15: 53,719,678 K87E probably benign Het
Sec16b T C 1: 157,560,754 S564P probably benign Het
Speer4a A T 5: 26,034,962 C263* probably null Het
Susd1 C A 4: 59,411,359 C158F possibly damaging Het
Vmn2r6 C T 3: 64,556,532 V294M probably benign Het
Other mutations in Gpr180
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02212:Gpr180 APN 14 118160176 missense probably damaging 1.00
IGL02449:Gpr180 APN 14 118160120 missense probably damaging 1.00
IGL02740:Gpr180 APN 14 118139749 missense probably damaging 1.00
IGL03103:Gpr180 APN 14 118139763 missense possibly damaging 0.95
R0321:Gpr180 UTSW 14 118148287 critical splice donor site probably null
R0545:Gpr180 UTSW 14 118160046 missense possibly damaging 0.77
R0688:Gpr180 UTSW 14 118148184 missense probably benign 0.00
R0844:Gpr180 UTSW 14 118157947 missense probably damaging 1.00
R2566:Gpr180 UTSW 14 118139773 missense probably benign 0.06
R5334:Gpr180 UTSW 14 118160056 missense probably damaging 0.99
R5378:Gpr180 UTSW 14 118139839 missense probably benign
R5583:Gpr180 UTSW 14 118162698 missense probably damaging 1.00
R6851:Gpr180 UTSW 14 118153625 missense probably damaging 1.00
R7382:Gpr180 UTSW 14 118162623 missense possibly damaging 0.63
Predicted Primers PCR Primer

Sequencing Primer
Posted On2017-07-14