Incidental Mutation 'R6062:Daglb'
ID483837
Institutional Source Beutler Lab
Gene Symbol Daglb
Ensembl Gene ENSMUSG00000039206
Gene Namediacylglycerol lipase, beta
Synonyms
MMRRC Submission 044227-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.137) question?
Stock #R6062 (G1)
Quality Score186.009
Status Validated
Chromosome5
Chromosomal Location143464584-143505942 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 143494603 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Threonine at position 454 (I454T)
Ref Sequence ENSEMBL: ENSMUSP00000043088 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000045593]
Predicted Effect probably benign
Transcript: ENSMUST00000045593
AA Change: I454T

PolyPhen 2 Score 0.070 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000043088
Gene: ENSMUSG00000039206
AA Change: I454T

DomainStartEndE-ValueType
transmembrane domain 20 42 N/A INTRINSIC
transmembrane domain 55 77 N/A INTRINSIC
transmembrane domain 97 119 N/A INTRINSIC
transmembrane domain 131 153 N/A INTRINSIC
Pfam:Lipase_3 370 505 1.1e-18 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000155383
Meta Mutation Damage Score 0.064 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.7%
  • 20x: 92.9%
Validation Efficiency 98% (56/57)
MGI Phenotype PHENOTYPE: Mice homozygous for null mutations have a reduction in endocannabinoids in the brain and a decrease in adult neuronal proliferation in the hippocampus. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
AI314180 G T 4: 58,826,453 S1038Y possibly damaging Het
Atat1 T A 17: 35,908,564 Q136L probably damaging Het
Atm A G 9: 53,488,587 L1531P probably damaging Het
Brca2 G A 5: 150,556,889 R2708H probably damaging Het
Cand1 G A 10: 119,218,010 A141V possibly damaging Het
Cdcp2 T C 4: 107,102,492 S35P probably damaging Het
Ces4a G A 8: 105,138,174 probably null Het
Colec10 T A 15: 54,459,807 M142K possibly damaging Het
Crtc1 G T 8: 70,406,189 D90E probably damaging Het
Csmd1 T C 8: 16,092,305 E1528G possibly damaging Het
Csnk2a2 A G 8: 95,457,469 V154A possibly damaging Het
Dtna C A 18: 23,622,056 N478K possibly damaging Het
E330034G19Rik A T 14: 24,293,380 probably benign Het
Eefsec A T 6: 88,355,629 S200T probably benign Het
Enthd1 T A 15: 80,452,715 D506V probably damaging Het
Erbb2 A G 11: 98,433,249 Y736C probably damaging Het
Gabrg1 A G 5: 70,780,713 C183R probably damaging Het
Ginm1 T A 10: 7,775,333 H103L probably benign Het
Golgb1 C A 16: 36,914,671 Q1427K possibly damaging Het
Grb14 G T 2: 65,022,620 Q9K possibly damaging Het
Hbp1 A T 12: 31,937,247 M192K probably damaging Het
Herpud2 A T 9: 25,108,988 D357E probably damaging Het
Idi1 A G 13: 8,887,505 S111G probably damaging Het
Lrrc32 T C 7: 98,498,541 V176A probably benign Het
Matn1 T A 4: 130,951,966 D310E probably benign Het
Mbtps1 A T 8: 119,531,091 L469Q possibly damaging Het
Muc4 A G 16: 32,759,308 D2417G unknown Het
Myo7b T C 18: 31,967,990 T1551A possibly damaging Het
Neb A G 2: 52,185,281 M224T probably benign Het
Ola1 A T 2: 73,199,498 D92E probably damaging Het
Olfr1019 T G 2: 85,841,114 I226L probably benign Het
Olfr388-ps1 A C 11: 73,724,560 C155G unknown Het
Olfr723 A T 14: 49,928,662 M294K probably damaging Het
Olfr912 T A 9: 38,539,144 M83K probably damaging Het
Otud4 T C 8: 79,673,896 Y1080H probably damaging Het
Plce1 G A 19: 38,524,751 A165T probably benign Het
Prelid2 T C 18: 41,912,465 I127V probably benign Het
Rab35 A G 5: 115,640,088 I38V probably damaging Het
Rab3d T C 9: 21,910,519 T209A probably benign Het
Rapgef1 A G 2: 29,700,732 E321G probably damaging Het
Rem1 T C 2: 152,628,097 M1T probably null Het
Rgsl1 T C 1: 153,799,872 K181R possibly damaging Het
Scel C A 14: 103,585,136 N395K possibly damaging Het
Sept9 A G 11: 117,290,800 E142G possibly damaging Het
Shcbp1 A C 8: 4,764,905 M191R probably benign Het
Slc22a19 T C 19: 7,674,282 N520S probably damaging Het
Slc28a1 C T 7: 81,115,563 R9* probably null Het
Slc2a7 T C 4: 150,168,427 V508A probably benign Het
Slc8a3 G A 12: 81,314,350 P565L probably damaging Het
Svil T G 18: 5,106,724 V1855G probably damaging Het
Tenm3 T G 8: 48,343,406 I455L possibly damaging Het
Tmem267 T C 13: 119,609,231 S141P probably damaging Het
Tnn T A 1: 160,098,278 D1383V probably damaging Het
Usp9y G T Y: 1,454,199 Q23K probably benign Het
Vmn1r46 T C 6: 89,976,259 I30T possibly damaging Het
Zfp382 T C 7: 30,133,590 L222P probably damaging Het
Zfp950 T A 19: 61,120,425 K73N possibly damaging Het
Other mutations in Daglb
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03046:Daglb UTSW 5 143501193 missense probably damaging 1.00
PIT4802001:Daglb UTSW 5 143503048 missense probably benign 0.04
R0145:Daglb UTSW 5 143474608 splice site probably benign
R0348:Daglb UTSW 5 143487196 missense probably benign 0.01
R0504:Daglb UTSW 5 143494197 missense probably benign
R1695:Daglb UTSW 5 143494606 missense probably benign 0.02
R4043:Daglb UTSW 5 143487151 missense possibly damaging 0.92
R4358:Daglb UTSW 5 143473134 intron probably benign
R4666:Daglb UTSW 5 143503349 missense probably damaging 0.99
R5609:Daglb UTSW 5 143478519 missense probably benign 0.00
R6153:Daglb UTSW 5 143503341 missense probably benign 0.00
R6251:Daglb UTSW 5 143489934 missense probably damaging 1.00
R6475:Daglb UTSW 5 143481651 missense probably benign
Predicted Primers PCR Primer
(F):5'- TGAGCCAAGCCTTCAGTGTC -3'
(R):5'- TTTACACAGCGCTGTAAACAC -3'

Sequencing Primer
(F):5'- AATTGAACTCGAGACCTCTGG -3'
(R):5'- CGCTGTAAACACACGTCTGAGG -3'
Posted On2017-07-14